LOCUS       NM_172402               2945 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Mus musculus solute carrier family 25, member 32 (Slc25a32), mRNA.
ACCESSION   NM_172402
VERSION     NM_172402.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Peng,M.Z., Shao,Y.X., Li,X.Z., Zhang,K.D., Cai,Y.N., Lin,Y.T.,
            Jiang,M.Y., Liu,Z.C., Su,X.Y., Zhang,W., Jiang,X.L. and Liu,L.
  TITLE     Mitochondrial FAD shortage in SLC25A32 deficiency affects
            folate-mediated one-carbon metabolism
  JOURNAL   Cell Mol Life Sci 79 (7), 375 (2022)
   PUBMED   35727412
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Kim,J., Lei,Y., Guo,J., Kim,S.E., Wlodarczyk,B.J., Cabrera,R.M.,
            Lin,Y.L., Nilsson,T.K., Zhang,T., Ren,A., Wang,L., Yuan,Z.,
            Zheng,Y.F., Wang,H.Y. and Finnell,R.H.
  TITLE     Formate rescues neural tube defects caused by mutations in Slc25a32
  JOURNAL   Proc Natl Acad Sci U S A 115 (18), 4690-4695 (2018)
   PUBMED   29666258
  REMARK    GeneRIF: These data demonstrate that the loss of functional
            Slc25a32 results in cranial neural tube defects (NTDs) in mice and
            has also been observed in a human NTD patient.
REFERENCE   3  (bases 1 to 2945)
  AUTHORS   Lawrence,S.A., Hackett,J.C. and Moran,R.G.
  TITLE     Tetrahydrofolate recognition by the mitochondrial folate
            transporter
  JOURNAL   J Biol Chem 286 (36), 31480-31489 (2011)
   PUBMED   21768094
REFERENCE   4  (bases 1 to 2945)
  AUTHORS   Titus,S.A. and Moran,R.G.
  TITLE     Retrovirally mediated complementation of the glyB phenotype.
            Cloning of a human gene encoding the carrier for entry of folates
            into mitochondria
  JOURNAL   J Biol Chem 275 (47), 36811-36817 (2000)
   PUBMED   10978331
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB641480.1, AK167501.1, BB308144.2 and AK145178.1.
            
            On Feb 22, 2008 this sequence version replaced NM_172402.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK145178.1, BC031874.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-191               BB641480.1         2-192
            192-2347            AK167501.1         2-2157
            2348-2854           BB308144.2         136-642
            2855-2945           AK145178.1         2815-2905
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 15.39 cM"
     gene            1..2945
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="solute carrier family 25, member 32"
                     /db_xref="GeneID:69906"
                     /db_xref="MGI:MGI:1917156"
     exon            1..369
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1166
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="mitochondrial folate transporter/carrier;
                     mitochondrial FAD transporter"
                     /codon_start=1
                     /product="solute carrier family 25 member 32"
                     /protein_id="NP_765990.2"
                     /db_xref="CCDS:CCDS27443.1"
                     /db_xref="GeneID:69906"
                     /db_xref="MGI:MGI:1917156"
                     /translation="MTGQGQSAAGSAAWSAVFRHVRYENLVAGVSGGVLSNLALHPLD
                     LVKIRFAVSDGLEVRPKYKGILHCLATIWKVDGLRGLYQGVTPNVWGAGLSWGLYFFF
                     YNAIKSYKTEGRAEQLEPLEYLVSAAEAGAMTLCITNPLWVTKTRLMLQYGGVASPSQ
                     RQYKGMFDALVKIYKYEGVRGLYKGFVPGLFGTSHGALQFMAYELLKLKYNKHINRLP
                     EAQLSTAEYISVAALSKIFAVAATYPYQVVRARLQDQHVSYGGVTDVITKTWRKEGIG
                     GFYKGIAPNLIRVTPACCITFVVYENVSHFLYDLREKKVS"
     exon            370..520
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     exon            521..606
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     exon            607..767
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     exon            768..881
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1027
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..2945
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2319..2324
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="hexamer: AATAAA"
     regulatory      2326..2331
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="hexamer: AATGAA"
     polyA_site      2348
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
     polyA_site      2354
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
     regulatory      2836..2841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="hexamer: ATTAAA"
     polyA_site      2859
                     /gene="Slc25a32"
                     /gene_synonym="2610043O12Rik; Mftc"
                     /note="major polyA site"
ORIGIN      
        1 ggggctaagc aagctccgct ctgaggtttc tcctactccc cgctttgccg cgggtgtgac
       61 ttgcctgcag cagcgtcccg ggctccggtc ccggaagtgg gctcgcgtcc tgcggttcca
      121 ggccccgggc cgcgacagtc gccacggtgg cggccactac atcacgcccc acgtcggcgg
      181 cgctggacgc cgtagtgtcg acgaacccgg agcccatgac aggccagggc cagtcggctg
      241 ccgggtcggc ggcgtggagc gcggtgttcc gccacgtccg gtacgagaac ctggtggctg
      301 gcgtgagtgg cggggtcttg tccaacctgg cgctgcaccc gctcgacctc gtgaagatcc
      361 gcttcgctgt gagtgatgga ctggaagtaa gaccaaaata taaaggaatt ttgcattgct
      421 tggctaccat ttggaaagtt gatggactac gaggacttta tcaaggagta accccgaatg
      481 tgtggggtgc cggtttatcc tggggactct actttttctt ttacaatgcc atcaaatcat
      541 ataagacaga gggaagagct gaacagttag agccattaga gtacctcgtc tcagctgctg
      601 aagctggagc catgactctg tgcattacaa acccattatg ggtgacgaaa actcgcctta
      661 tgttacaata tggtggtgtt gctagccctt cacagagaca gtataaagga atgtttgatg
      721 cacttgtgaa aatatataaa tatgaaggtg tgcgtggatt atacaaggga tttgtccctg
      781 ggctgtttgg aacatcacat ggtgcccttc agtttatggc atatgagttg ctaaagttga
      841 agtacaacaa acacatcaat agattaccgg aagcccagct gagtacagca gaatacatct
      901 ctgtcgcagc gctatccaaa atatttgccg tagcagcaac atacccgtat caggttgtga
      961 gagcccgcct tcaggatcag catgtgtctt atggtggtgt aacagatgtg atcacaaaga
     1021 cgtggaggaa agaaggcatc ggtggatttt acaaaggaat tgcccccaat ctcattagag
     1081 tgactccagc ctgctgcatc acctttgtgg tttatgaaaa tgtctctcac tttttatatg
     1141 accttagaga aaagaaagtg agctaaaaga agatcccagt ctatgttctt gaggcaacag
     1201 caaagtcctt tgtgtttgaa gcgcaaagct cagaagagtt ctgcagagct acatagctgg
     1261 taaccccatg tctgtgttac aagtgaaaga actgctaagt catcttgatg tcttctgctc
     1321 tttgcatctc cgtgtgtgtg gagcttggca gcctctacct ggagtgggta ccatctgcct
     1381 gacgttgcaa ctgatagcca cggcgaaaat gtacaggatc ttccattttg ttattcaggg
     1441 agaagctcat ttgcaatatt tgtaaataat ttgatgaatg ttcttttttt ctgaactctc
     1501 ttgcctggat tggctttaaa actgaccatg tgcaatagca aggaactggc ttcttacaag
     1561 aatgactata tttcaagggt aaattaaact tggggaactt ataataaggt attatctaac
     1621 tctagcccat tttgaatggg gatatttttg ttagaaagaa gtctgttata gacgtgcttt
     1681 agttttaatc caactggaac atactaagta cagaaattgg agaagtcatg gatttctctg
     1741 agaaatatct agcacctttt gccattccat tgcttaatta tttacagtga gattctggac
     1801 gcagaccttg tattttggcc ataaacactt agtgtgtggt tagggtgtat aaaacctgaa
     1861 cttgcaggat ccggtgaagc ctttatttag catgtgtggc tacaaatcac tcatcagaaa
     1921 tgctggctca tgtttcacag agatggaatg tttgatgtaa gcttgttgaa aaactgaata
     1981 gaaacaagca ttctagtttc ccttcatttt tagatttatg gttccagatt ccaaataatt
     2041 atttgtctgc attggttgct caaattcagg ttgaaataca aattacattt gtctgtggcc
     2101 attactggtt tgatttctaa gtcattgtag atctccaggt aaaaggcctg ctcccgaagg
     2161 atgctagact aatactaact cttctgatga gcaactgtca gtactgttgc actagcctct
     2221 attttttgta aataaatcat tataatcaaa gctgtattta aagggttctt tgaaaataag
     2281 cctatttttt tatattttgc aaatggaatt gtttttaaaa taaaaaatga aaagataatt
     2341 tatatttaca aaaatggtgt ttgctataat atcctttacc tttatttttt aagtatgttt
     2401 gtttattcag caaaattgat acattttaaa atcacgatac actgtaacaa aaagcaagtt
     2461 tttcagcatt tacaaattcc tggcatataa ctctttgaaa tttggggaca atgaatcaat
     2521 atttccagga attcttagaa atttcaaaat tactatttta aacacagtca tgactatatc
     2581 tacactttat ttgggtatct caaatttttg ttacagaata aaatacactg aatttagtgt
     2641 aagttagtta cttatatagt aagtaaatac aaagaactac agcctacagg aatatttaaa
     2701 caatgagaaa tataatttgc aattcagatc tcccaggtaa ataacttcag aatgttttat
     2761 aaattatgca tttcgtagtt gcatggggtt caccttgata cttatgtctg tatgggaagg
     2821 caagctatgg ttgatattaa agttttagaa gtagaacaat cacttgagtt ctatctgcct
     2881 caaattaata agacaattct tatcctctga aagattataa acgtgcataa taaatgtgtt
     2941 actgc
//
