LOCUS       NM_020520               1746 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus solute carrier family 25 (mitochondrial
            carnitine/acylcarnitine translocase), member 20 (Slc25a20), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_020520 NM_175705 XM_910468
VERSION     NM_020520.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Paredes,A., Justo-Mendez,R., Jimenez-Blasco,D., Nunez,V.,
            Calero,I., Villalba-Orero,M., Alegre-Marti,A., Fischer,T.,
            Gradillas,A., Sant'Anna,V.A.R., Were,F., Huang,Z.,
            Hernansanz-Agustin,P., Contreras,C., Martinez,F., Camafeita,E.,
            Vazquez,J., Ruiz-Cabello,J., Area-Gomez,E., Sanchez-Cabo,F.,
            Treuter,E., Bolanos,J.P., Estebanez-Perpina,E., Ruperez,F.J.,
            Barbas,C., Enriquez,J.A. and Ricote,M.
  TITLE     gamma-Linolenic acid in maternal milk drives cardiac metabolic
            maturation
  JOURNAL   Nature 618 (7964), 365-373 (2023)
   PUBMED   37225978
  REMARK    Erratum:[Nature. 2023 Jul;619(7968):E24. doi:
            10.1038/s41586-023-06316-w.. PMID: 37322272]
REFERENCE   3  (bases 1 to 1746)
  AUTHORS   Ghnaimawi,S., Zhang,S., Baum,J.I. and Huang,Y.
  TITLE     The Effects of Maternal Intake of EPA and DHA Enriched Diet During
            Pregnancy and Lactation on Offspring's Muscle Development and
            Energy Homeostasis
  JOURNAL   Front Physiol 13, 881624 (2022)
   PUBMED   35733999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1746)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1746)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1746)
  AUTHORS   Gacias,M., Perez-Marti,A., Pujol-Vidal,M., Marrero,P.F., Haro,D.
            and Relat,J.
  TITLE     PGC-1beta regulates mouse carnitine-acylcarnitine translocase
            through estrogen-related receptor alpha
  JOURNAL   Biochem Biophys Res Commun 423 (4), 838-843 (2012)
   PUBMED   22713466
  REMARK    GeneRIF: the 50-flanking region of the Cact gene contains a
            consensus sequence for ERRalpha. This sequence binds ERRa both in
            vivo and in vitro and is required for the activation of Cact
            expression by the PGC-1/ERR axis
REFERENCE   7  (bases 1 to 1746)
  AUTHORS   Gutgesell,A., Wen,G., Konig,B., Koch,A., Spielmann,J., Stangl,G.I.,
            Eder,K. and Ringseis,R.
  TITLE     Mouse carnitine-acylcarnitine translocase (CACT) is
            transcriptionally regulated by PPARalpha and PPARdelta in liver
            cells
  JOURNAL   Biochim Biophys Acta 1790 (10), 1206-1216 (2009)
   PUBMED   19577614
  REMARK    GeneRIF: Data show that the upregulation of CACT by PPARalpha and
            PPARdelta may increase the import of acylcarnitine into the
            mitochondrial matrix during fasting.
REFERENCE   8  (bases 1 to 1746)
  AUTHORS   Ramsay,R.R., Gandour,R.D. and van der Leij,F.R.
  TITLE     Molecular enzymology of carnitine transfer and transport
  JOURNAL   Biochim Biophys Acta 1546 (1), 21-43 (2001)
   PUBMED   11257506
  REMARK    Review article
REFERENCE   9  (bases 1 to 1746)
  AUTHORS   Huizing,M., Iacobazzi,V., Ijlst,L., Savelkoul,P., Ruitenbeek,W.,
            van den Heuvel,L., Indiveri,C., Smeitink,J., Trijbels,F.,
            Wanders,R. and Palmieri,F.
  TITLE     Cloning of the human carnitine-acylcarnitine carrier cDNA and
            identification of the molecular defect in a patient
  JOURNAL   Am J Hum Genet 61 (6), 1239-1245 (1997)
   PUBMED   9399886
REFERENCE   10 (bases 1 to 1746)
  AUTHORS   Indiveri,C., Tonazzi,A., Dierks,T., Kramer,R. and Palmieri,F.
  TITLE     The mitochondrial carnitine carrier: characterization of SH-groups
            relevant for its transport function
  JOURNAL   Biochim Biophys Acta 1140 (1), 53-58 (1992)
   PUBMED   1420325
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT571271.12.
            
            On Jun 19, 2018 this sequence version replaced NM_020520.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152436.1, AK075624.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-197               CT571271.12        90352-90548
            198-290             CT571271.12        97982-98074
            291-418             CT571271.12        100635-100762
            419-509             CT571271.12        105800-105890
            510-627             CT571271.12        108303-108420
            628-700             CT571271.12        110188-110260
            701-810             CT571271.12        110563-110672
            811-935             CT571271.12        111265-111389
            936-1746            CT571271.12        112048-112858
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.61 cM"
     gene            1..1746
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /note="solute carrier family 25 (mitochondrial
                     carnitine/acylcarnitine translocase), member 20"
                     /db_xref="GeneID:57279"
                     /db_xref="MGI:MGI:1928738"
     exon            1..197
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..998
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="mitochondrial carnitine/acylcarnitine carrier
                     protein"
                     /protein_id="NP_065266.1"
                     /db_xref="CCDS:CCDS23535.1"
                     /db_xref="GeneID:57279"
                     /db_xref="MGI:MGI:1928738"
                     /translation="MADEPKPISPFKNLLAGGFGGMCLVFVGHPLDTVKVRLQTQPPS
                     LSGQPPMYSGTLDCFRKTLMREGITGLYRGMAAPIIGVTPMFAVCFFGFGLGKKLQQK
                     SPEDELSYPQLFTAGMLSGVFTTGIMTPGERIKCLLQIQASSGENKYSGTLDCAKKLY
                     QEFGIRGFYKGTVLTLMRDVPASGMYFMTYEWLKNLFTPEGKSVSDLSVPRILVAGGF
                     AGIFNWAVAIPPDVLKSRFQTAPPGKYPNGFRDVLRELIREEGVTSLYKGFNAVMIRA
                     FPANAACFLGFEIAMKFLNWIAPNL"
     exon            198..290
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            291..418
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            419..509
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            510..627
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            628..700
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            701..810
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            811..935
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1746
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1724..1729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /note="hexamer: AATAAA"
     polyA_site      1745
                     /gene="Slc25a20"
                     /gene_synonym="1110007P09Rik; CAC; Cact; mCAC"
                     /note="major polyA site"
ORIGIN      
        1 gaatgtaggt gaaaggtcgg cggagccgct cgggcagcgg agcaagacca agactgcccg
       61 gagctgacag acagacagac agaagactga gcatggcaga cgagccgaaa cccatcagtc
      121 cgtttaagaa cctcctggct ggcggcttcg gcggcatgtg cctggtgttt gtggggcacc
      181 ccctggacac ggtcaaggtc cgactgcaga cacaaccacc aagtttgtct ggacagccac
      241 ctatgtactc tgggaccttg gactgtttcc ggaagactct tatgagagag ggcatcacag
      301 ggctgtatcg gggcatggct gcgcccatca ttggagtcac ccctatgttc gccgtgtgct
      361 tctttgggtt tggtctgggg aagaaactac agcagaaatc tccagaggat gaacttagct
      421 acccacagct gtttacagct gggatgttat ctggtgtgtt caccacagga atcatgaccc
      481 ctggagaacg gatcaaatgc ttactgcaga ttcaggcttc ttcaggggag aacaagtaca
      541 gtggcacctt ggactgcgca aagaagctgt atcaggagtt cgggatccgc ggcttctaca
      601 aagggactgt gctcacactc atgcgagatg ttcctgccag tgggatgtat ttcatgacat
      661 atgaatggct taaaaatctc ttcactccag agggaaagag tgtcagtgac ctcagcgtgc
      721 ctcggatcct ggtggctggt ggctttgcag ggatcttcaa ctgggccgtg gcaatccccc
      781 cggacgtgct caagtctcga ttccagactg cacctcctgg aaaatatcct aatggtttca
      841 gagatgtgtt gagagagctg atccgagaag aaggagtcac ctccttgtac aaagggttca
      901 atgcagtcat gatccgcgcc ttcccagcca acgctgcctg tttccttggc tttgaaattg
      961 ccatgaagtt ccttaattgg attgccccca acttgtgaag ctgaagactc ttcaagacct
     1021 gtggatgctg gacactgttg ctgagaggca ggggcaggag gaggaagtcc tgagagggag
     1081 gggagggaag aggtgtgatc agagctccag gcttggctgt gatggtgaaa ctgttgcctt
     1141 aatgatatcc tctaccttgt ataacttggt gccattttga aacttgaatt tagaggattt
     1201 ccagagaatt ggagatggaa taaatagttt atagactcga tacctttggc caaattgcca
     1261 cctactggtt gactgaaggc cctgttacac tcaaaatact ccttcccgaa gaggtgatgg
     1321 ctgactctct ggaggcccct tgcctacttt cagccagcta aaccaggagc tgggatcttt
     1381 aattgctagc caggaaaact caagagatgt ctctggtgcc atccatatac tcctggctgt
     1441 gcatgggatg tgggtgtgaa gcacacatct gtctatccaa tggactgggt agataatacc
     1501 taagaacagc ccacctacta accatttttt tttgatattt ttacacaaga aataataaga
     1561 agaaagctac aattgtctaa atccccagga gaaagtagag aattttccct cctacacagt
     1621 gaagacagtc ccagcctcct gggataagtg tgagagccat aatgtatgaa ggctcctttt
     1681 gcacattctt tccccatgag agcactcagt tttaacagga aacaataaat attgtttcta
     1741 attttt
//
