LOCUS       NM_172436               2949 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Mus musculus solute carrier family 25 (mitochondrial carrier,
            Aralar), member 12 (Slc25a12), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_172436
VERSION     NM_172436.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Gonzalez-Moreno,L., Santamaria-Cano,A., Paradela,A.,
            Martinez-Chantar,M.L., Martin,M.A., Perez-Carreras,M.,
            Garcia-Picazo,A., Vazquez,J., Calvo,E., Gonzalez-Aseguinolaza,G.,
            Saheki,T., Del Arco,A., Satrustegui,J. and Contreras,L.
  TITLE     Exogenous aralar/slc25a12 can replace citrin/slc25a13 as malate
            aspartate shuttle component in liver
  JOURNAL   Mol Genet Metab Rep 35, 100967 (2023)
   PUBMED   36967723
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2949)
  AUTHORS   Perez-Liebana,I., Juaristi,I., Gonzalez-Sanchez,P.,
            Gonzalez-Moreno,L., Rial,E., Podunavac,M., Zakarian,A., Molgo,J.,
            Vallejo-Illarramendi,A., Mosqueira-Martin,L., Lopez de Munain,A.,
            Pardo,B., Satrustegui,J. and Del Arco,A.
  TITLE     A Ca2+-Dependent Mechanism Boosting Glycolysis and OXPHOS by
            Activating Aralar-Malate-Aspartate Shuttle, upon Neuronal
            Stimulation
  JOURNAL   J Neurosci 42 (19), 3879-3895 (2022)
   PUBMED   35387872
REFERENCE   4  (bases 1 to 2949)
  AUTHORS   Perez-Liebana,I., Casarejos,M.J., Alcaide,A., Herrada-Soler,E.,
            Llorente-Folch,I., Contreras,L., Satrustegui,J. and Pardo,B.
  TITLE     betaOHB Protective Pathways in Aralar-KO Neurons and Brain: An
            Alternative to Ketogenic Diet
  JOURNAL   J Neurosci 40 (48), 9293-9305 (2020)
   PUBMED   33087477
REFERENCE   5  (bases 1 to 2949)
  AUTHORS   Petralla,S., Pena-Altamira,L.E., Poeta,E., Massenzio,F.,
            Virgili,M., Barile,S.N., Sbano,L., Profilo,E., Corricelli,M.,
            Danese,A., Giorgi,C., Ostan,R., Capri,M., Pinton,P., Palmieri,F.,
            Lasorsa,F.M. and Monti,B.
  TITLE     Deficiency of Mitochondrial Aspartate-Glutamate Carrier 1 Leads to
            Oligodendrocyte Precursor Cell Proliferation Defects Both In Vitro
            and In Vivo
  JOURNAL   Int J Mol Sci 20 (18), 4486 (2019)
   PUBMED   31514314
  REMARK    GeneRIF: AGC1 impairment alters myelination not only by acting on
            N-acetyl-aspartate production in neurons but also on
            Oligodendrocyte Precursor Cell proliferation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2949)
  AUTHORS   Jalil,M.A., Begum,L., Contreras,L., Pardo,B., Iijima,M., Li,M.X.,
            Ramos,M., Marmol,P., Horiuchi,M., Shimotsu,K., Nakagawa,S.,
            Okubo,A., Sameshima,M., Isashiki,Y., Del Arco,A., Kobayashi,K.,
            Satrustegui,J. and Saheki,T.
  TITLE     Reduced N-acetylaspartate levels in mice lacking aralar, a brain-
            and muscle-type mitochondrial aspartate-glutamate carrier
  JOURNAL   J Biol Chem 280 (35), 31333-31339 (2005)
   PUBMED   15987682
  REMARK    GeneRIF: These results show that aralar plays an important role in
            myelin formation by providing aspartate for the synthesis of
            N-acetylaspartate in neuronal cells.
REFERENCE   7  (bases 1 to 2949)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   8  (bases 1 to 2949)
  AUTHORS   Sinasac,D.S., Moriyama,M., Jalil,M.A., Begum,L., Li,M.X.,
            Iijima,M., Horiuchi,M., Robinson,B.H., Kobayashi,K., Saheki,T. and
            Tsui,L.C.
  TITLE     Slc25a13-knockout mice harbor metabolic deficits but fail to
            display hallmarks of adult-onset type II citrullinemia
  JOURNAL   Mol Cell Biol 24 (2), 527-536 (2004)
   PUBMED   14701727
REFERENCE   9  (bases 1 to 2949)
  AUTHORS   Ramos,M., del Arco,A., Pardo,B., Martinez-Serrano,A.,
            Martinez-Morales,J.R., Kobayashi,K., Yasuda,T., Bogonez,E.,
            Bovolenta,P., Saheki,T. and Satrustegui,J.
  TITLE     Developmental changes in the Ca2+-regulated mitochondrial
            aspartate-glutamate carrier aralar1 in brain and prominent
            expression in the spinal cord
  JOURNAL   Brain Res Dev Brain Res 143 (1), 33-46 (2003)
   PUBMED   12763579
REFERENCE   10 (bases 1 to 2949)
  AUTHORS   del Arco,A., Morcillo,J., Martinez-Morales,J.R., Galian,C.,
            Martos,V., Bovolenta,P. and Satrustegui,J.
  TITLE     Expression of the aspartate/glutamate mitochondrial carriers
            aralar1 and citrin during development and in adult rat tissues
  JOURNAL   Eur J Biochem 269 (13), 3313-3320 (2002)
   PUBMED   12084073
  REMARK    GeneRIF: Expression of the aspartate/glutamate mitochondrial
            carriers aralar1 and citrin during development
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX284624.4.
            
            On Jan 5, 2022 this sequence version replaced NM_172436.4.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083156.1, AK144958.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                BX284624.4         143595-143636       c
            43-96               BX284624.4         142609-142662       c
            97-239              BX284624.4         119972-120114       c
            240-355             BX284624.4         109687-109802       c
            356-495             BX284624.4         100058-100197       c
            496-642             BX284624.4         91065-91211         c
            643-781             BX284624.4         88507-88645         c
            782-875             BX284624.4         87545-87638         c
            876-960             BX284624.4         84138-84222         c
            961-1042            BX284624.4         74652-74733         c
            1043-1201           BX284624.4         72747-72905         c
            1202-1254           BX284624.4         69754-69806         c
            1255-1335           BX284624.4         69442-69522         c
            1336-1476           BX284624.4         58552-58692         c
            1477-1615           BX284624.4         55598-55736         c
            1616-1774           BX284624.4         52723-52881         c
            1775-1865           BX284624.4         52514-52604         c
            1866-2949           BX284624.4         50352-51435         c
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 42.38 cM"
     gene            1..2949
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /note="solute carrier family 25 (mitochondrial carrier,
                     Aralar), member 12"
                     /db_xref="GeneID:78830"
                     /db_xref="MGI:MGI:1926080"
     exon            1..42
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..2064
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /note="calcium-binding mitochondrial carrier protein
                     Aralar1; mitochondrial aspartate glutamate carrier 1"
                     /codon_start=1
                     /product="electrogenic aspartate/glutamate antiporter
                     SLC25A12, mitochondrial"
                     /protein_id="NP_766024.1"
                     /db_xref="CCDS:CCDS16113.1"
                     /db_xref="GeneID:78830"
                     /db_xref="MGI:MGI:1926080"
                     /translation="MAVKVHTTKRGDPHELRNIFLQYASTEVDGEHYMTPEDFVQRYL
                     GLYNDPNSNPKIVQLLAGVADQTKDGLISYQEFLAFESVLCAPDSMFIVAFQLFDKSG
                     NGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHNRKKHLNYVEFTQFLQELQLE
                     HARQAFALKDKSKSGMISGLDFSDVMVTIRSHMLTPFVEENLVSAAGGGTSHQVSFSY
                     FNAFNSLLNNMELVRKIYSTLAGTRKDIEVTKEEFAQSAIRYGQVTPLEIDILYQLAD
                     LYNASGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGS
                     VAGAVGATAVYPIDLVKTRMQNQRGTGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGL
                     IPQLIGVAPEKAIKLTVNDFVRDKFTKRDGSIPLPAEILAGGCAGGSQVIFTNPLEIV
                     KIRLQVAGEITTGPRVSALNVLQDLGLFGLYKGAKACFLRDIPFSAIYFPVYAHCKLL
                     LADENGRVGGINLLTAGALAGVPAASLVTPADVIKTRLQVAARAGQTTYSGVVDCFRK
                     ILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPSGSEPTPKSR
                     IADLPPANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVAQPKAAAAAQ"
     exon            43..96
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            97..239
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            240..355
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            356..495
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            496..642
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            643..781
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            782..875
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            876..960
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1042
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1201
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1254
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1335
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1476
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1615
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1774
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1865
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..2949
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2456..2461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2476
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /note="major polyA site"
     regulatory      2928..2933
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2949
                     /gene="Slc25a12"
                     /gene_synonym="2610002D09Rik; B230107K20Rik"
ORIGIN      
        1 actcgctcgt tcccggcttc cgagcacagc atggcggtca aggtgcatac aaccaaacga
       61 ggtgatcctc atgagctgag aaacatattc ctccagtatg ccagcactga ggtggacggg
      121 gagcattaca tgactccaga agacttcgtt caacgctatc ttggcctgta caacgatcca
      181 aacagcaacc caaagattgt gcagctcttg gcaggagtag ctgatcaaac caaggatggg
      241 ttgatctcct atcaagagtt tttggcattt gaatctgttc tatgtgctcc agattccatg
      301 ttcatagtgg ctttccaact gtttgacaag agcggaaatg gagaggtgac atttgaaaat
      361 gtcaaggaga tttttgggca gactattatc catcaccaca tcccttttaa ctgggactgc
      421 gagttcatcc ggctgcactt tgggcacaac cgcaagaagc acctgaacta tgtggaattc
      481 actcagttcc tccaggaatt gcaattggaa cacgcaaggc aagcctttgc actgaaagac
      541 aagagcaaaa gcggcatgat ctcgggtctg gatttcagtg acgtcatggt caccatccgg
      601 tcccacatgc tcactccttt tgtggaggag aacctggtct cagctgccgg agggggtacc
      661 tcacaccaag ttagcttctc ctacttcaac gcatttaact ccttactcaa caacatggag
      721 cttgttcgaa agatctatag cactttagca ggcacgagga aagatattga agtcaccaag
      781 gaggaatttg cccagagcgc catacgctat ggacaggtca ctccactaga aattgatatc
      841 ttgtaccagc ttgcagacct atataatgcc tcagggcgct tgactctggc agacatcgag
      901 agaatagctc cgctggccga gggggcctta ccttacaacc tggcagagct tcagaggcag
      961 caatctcctg gcttgggcag acctatctgg ctccagattg ccgagtctgc atacaggttc
     1021 actctgggct cagttgctgg agctgtgggg gccactgcgg tgtaccccat agacctggtg
     1081 aagacccgga tgcaaaacca gcgtggtacg ggctctgtgg tcggggagct gatgtataaa
     1141 aacagcttcg actgttttaa gaaagtgttg cgctacgagg gtttctttgg actgtaccga
     1201 ggtctgatcc cacagctgat aggggttgca ccagaaaaag ccattaagct aacagttaat
     1261 gattttgtcc gggacaaatt taccaaaaga gatggctcta tcccactgcc ggcggaaatc
     1321 cttgctggag gttgcgctgg aggctcgcag gtcatcttta ccaaccctct ggagatagtg
     1381 aagatccgcc tgcaggtagc cggagagatc accacaggac cgagagtcag tgctctgaat
     1441 gtgcttcagg acctgggact tttcgggctg tacaagggtg ccaaagcctg tttcctccga
     1501 gacattccct tctccgcaat ctattttccc gtgtacgctc actgcaaact cctcctggct
     1561 gatgagaacg gacgcgtggg aggcatcaat ctcctgacag cgggagcctt ggcaggcgta
     1621 cctgctgctt ctctggtgac ccctgctgat gtcatcaaga caagactgca agtggccgcc
     1681 agggccggcc agacaacata cagcggggtc gtcgactgtt tccgaaagat tctccgggaa
     1741 gaagggccct cggcattttg gaaagggact gcagctcggg tgtttcgatc ctcacctcag
     1801 tttggtgtca ctctggttac ctacgagctt ctgcagcggt ggttttacat agattttgga
     1861 ggcctcaaac cttctggctc agaaccaacg ccaaagtccc gcatcgcaga cctccctcct
     1921 gccaaccccg atcacatcgg tggctacaga cttgccacgg ccactttcgc tggcattgaa
     1981 aacaagtttg gcctttacct ccccaagttt aagtctccta gcgtagctgt ggctcagccc
     2041 aaggcggcag cagcagccca atgacatagc aagtacagac cgaggcccag gagaacgggc
     2101 ggtaccgtgt ccagtcagct gcatggtact gtcagcgctg gggtcaacca ctttctatac
     2161 gacctataca tgtattgttt ataaagtgat catacgccca gggagagccg cagtgcagtt
     2221 tcaagcttta gtctttacgt gttgaaatgt tttctgagcc ttggcatgaa ttacgtgttc
     2281 tagactctgc ttcgcacagc ttgtacctac agtgactgta catattgtac atctttgtac
     2341 agagacacct cggcacctca tcccaacaaa tcacgtttat aaacatgaaa cgctgttctg
     2401 agtggcttga actgtacaga aggttttgaa agtgttttat tatgaatcat gtaagaataa
     2461 atgtactaaa gttaaattca ttctcctcct gatgacttat ggaaatcaag cagccatgtt
     2521 tgaatgtatc cagtttcagt ttgttttcca ttcgcgacta ggaaggtatt tgctgataca
     2581 aggcagagcg aaacttaatg ctcaccacat taaataagaa acgcgtggga agggtgcttg
     2641 cccgtggatg ccagaggatg agccggtgac tcacgggctg ctgtgtttcc agtcgtttgt
     2701 agctgagtgt aatcctcctg tcagctggaa gcagctcctc atgcgcgcgc gcacacacac
     2761 acacacacac acacacacac acacacacac acacacacac acgaacggga tgggctccag
     2821 caaggctcgc ttagttctgc ttttctccta tgcagcaagg tcaaggtcag gatgttgcaa
     2881 gagtaaagtt tggaagtttc ccttcgtaaa gtatgatacg aatcaaaaat aaaagcacac
     2941 caaaatgtc
//
