LOCUS       NM_001411470            3879 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 22 (organic anion transporter),
            member 8 (Slc22a8), transcript variant 4, mRNA.
ACCESSION   NM_001411470 XM_011247171
VERSION     NM_001411470.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Granados,J.C., Richelle,A., Gutierrez,J.M., Zhang,P., Zhang,X.,
            Bhatnagar,V., Lewis,N.E. and Nigam,S.K.
  TITLE     Coordinate regulation of systemic and kidney tryptophan metabolism
            by the drug transporters OAT1 and OAT3
  JOURNAL   J Biol Chem 296, 100575 (2021)
   PUBMED   33757768
  REMARK    GeneRIF: Coordinate regulation of systemic and kidney tryptophan
            metabolism by the drug transporters OAT1 and OAT3.
REFERENCE   3  (bases 1 to 3879)
  AUTHORS   Bush,K.T., Singh,P. and Nigam,S.K.
  TITLE     Gut-derived uremic toxin handling in vivo requires OAT-mediated
            tubular secretion in chronic kidney disease
  JOURNAL   JCI Insight 5 (7), 133817 (2020)
   PUBMED   32271169
  REMARK    GeneRIF: Gut-derived uremic toxin handling in vivo requires
            OAT-mediated tubular secretion in chronic kidney disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3879)
  AUTHORS   Nigam,A.K., Li,J.G., Lall,K., Shi,D., Bush,K.T., Bhatnagar,V.,
            Abagyan,R. and Nigam,S.K.
  TITLE     Unique metabolite preferences of the drug transporters OAT1 and
            OAT3 analyzed by machine learning
  JOURNAL   J Biol Chem 295 (7), 1829-1842 (2020)
   PUBMED   31896576
  REMARK    GeneRIF: Unique metabolite preferences of the drug transporters
            OAT1 and OAT3 analyzed by machine learning.
REFERENCE   5  (bases 1 to 3879)
  AUTHORS   Fu,Y., Breljak,D., Onishi,A., Batz,F., Patel,R., Huang,W., Song,P.,
            Freeman,B., Mayoux,E., Koepsell,H., Anzai,N., Nigam,S.K.,
            Sabolic,I. and Vallon,V.
  TITLE     Organic anion transporter OAT3 enhances the glucosuric effect of
            the SGLT2 inhibitor empagliflozin
  JOURNAL   Am J Physiol Renal Physiol 315 (2), F386-F394 (2018)
   PUBMED   29412698
  REMARK    GeneRIF: OAT3 increases the glucosuric effect of empagliflozin,
            which may relate to the partial overlap of its localization with
            SGLT2 and thus OAT3-mediated tubular secretion of empagliflozin in
            the early proximal tubule.
REFERENCE   6  (bases 1 to 3879)
  AUTHORS   Floyd,J.A., Gold,D.A., Concepcion,D., Poon,T.H., Wang,X.,
            Keithley,E., Chen,D., Ward,E.J., Chinn,S.B., Friedman,R.A.,
            Yu,H.T., Moriwaki,K., Shiroishi,T. and Hamilton,B.A.
  TITLE     A natural allele of Nxf1 suppresses retrovirus insertional
            mutations
  JOURNAL   Nat Genet 35 (3), 221-228 (2003)
   PUBMED   14517553
REFERENCE   7  (bases 1 to 3879)
  AUTHORS   Sweet,D.H., Miller,D.S., Pritchard,J.B., Fujiwara,Y., Beier,D.R.
            and Nigam,S.K.
  TITLE     Impaired organic anion transport in kidney and choroid plexus of
            organic anion transporter 3 (Oat3 (Slc22a8)) knockout mice
  JOURNAL   J Biol Chem 277 (30), 26934-26943 (2002)
   PUBMED   12011098
  REMARK    GeneRIF: Oat3 plays a key role in systemic detoxification and in
            control of the organic anion distribution in cerebrospinal fluid
            (Oat3 = SLC22A8 transporter)
REFERENCE   8  (bases 1 to 3879)
  AUTHORS   Pavlova,A., Sakurai,H., Leclercq,B., Beier,D.R., Yu,A.S. and
            Nigam,S.K.
  TITLE     Developmentally regulated expression of organic ion transporters
            NKT (OAT1), OCT1, NLT (OAT2), and Roct
  JOURNAL   Am J Physiol Renal Physiol 278 (4), F635-F643 (2000)
   PUBMED   10751225
REFERENCE   9  (bases 1 to 3879)
  AUTHORS   Brady,K.P., Dushkin,H., Fornzler,D., Koike,T., Magner,F., Her,H.,
            Gullans,S., Segre,G.V., Green,R.M. and Beier,D.R.
  TITLE     A novel putative transporter maps to the osteosclerosis (oc)
            mutation and is not expressed in the oc mutant mouse
  JOURNAL   Genomics 56 (3), 254-261 (1999)
   PUBMED   10087192
REFERENCE   10 (bases 1 to 3879)
  AUTHORS   Heaney,C., Shalev,H., Elbedour,K., Carmi,R., Staack,J.B.,
            Sheffield,V.C. and Beier,D.R.
  TITLE     Human autosomal recessive osteopetrosis maps to 11q13, a position
            predicted by comparative mapping of the murine osteosclerosis (oc)
            mutation
  JOURNAL   Hum Mol Genet 7 (9), 1407-1410 (1998)
   PUBMED   9700194
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125136.4 and AC025794.9.
            
            On Aug 25, 2022 this sequence version replaced XM_011247171.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1633286.1,
                                           SRR7652917.236793.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-172               AC125136.4         132703-132874
            173-683             AC125136.4         134200-134710
            684-1040            AC125136.4         135301-135657
            1041-1144           AC125136.4         146208-146311
            1145-1299           AC125136.4         146812-146966
            1300-1468           AC125136.4         147443-147611
            1469-1592           AC125136.4         149268-149391
            1593-1708           AC125136.4         149576-149691
            1709-1923           AC025794.9         71899-72113
            1924-2032           AC025794.9         72432-72540
            2033-2236           AC025794.9         72674-72877
            2237-3879           AC025794.9         72964-74606
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 5.44 cM"
     gene            1..3879
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /note="solute carrier family 22 (organic anion
                     transporter), member 8"
                     /db_xref="GeneID:19879"
                     /db_xref="MGI:MGI:1336187"
     exon            1..172
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            173..683
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            684..1040
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     CDS             708..2321
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /note="isoform 1 is encoded by transcript variant 4;
                     organic anion transporter 3; solute carrier family 22,
                     member 8; reduced in osteosclerosis transporter; organic
                     anion/dicarboxylate exchanger"
                     /codon_start=1
                     /product="organic anion transporter 3 isoform 1"
                     /protein_id="NP_001398399.1"
                     /db_xref="GeneID:19879"
                     /db_xref="MGI:MGI:1336187"
                     /translation="MTFSEILDRVGSMGPFQYLHVTLLALPILGIANHNLLQIFTATT
                     PDHHCRPPPNASLEPWVLPLGPNGKPEKCLRFVHLPNASLPNDTQGATEPCLDGWIYN
                     STRDTIVTEWDLVCGSNKLKEMAQSVFMAGILVGGPVFGELSDRFGRKPILTWSYLLL
                     AASGSSAAFSPSLTVYMIFRFLCGCSISGISLSTIILNVEWVPTSTRAISSTTIGYCY
                     TIGQFILPGLAYAVPQWRWLQLSVSAAFFIFSLLSWWVPESIRWLVLSGKFSKALKTL
                     QRVATFNGKKEEGEKLTVEELKFNLQKDITSAKVKYGLSDLFRVSILRRVTFCLSLAW
                     FATGFAYYSLAMGVEEFGVNIYILQIIFGGVDIPAKFITILSISYLGRRITQGFLLIL
                     AGVAILALIFVSSEMQLLRTALAVFGKGCLSGSFSCLFLYTSELYPTVLRQTGMGISN
                     IWARVGSMIAPLVKITGELQPFIPNVIFGTMTLLGGSAAFFLLETLNRPLPETIEDIQ
                     DWYQQTKKTKQEPEAEKASQTIPLKTGGP"
     exon            1041..1144
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1299
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1468
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1592
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1708
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1923
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2032
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2236
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..3879
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2708..2713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /note="hexamer: AATAAA"
     polyA_site      2730
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /note="major polyA site"
     regulatory      3860..3865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
                     /note="hexamer: CATAAA"
     polyA_site      3879
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; Roct"
ORIGIN      
        1 agaacagggg gttagctgca gcagcccagc ttgctgcaag cctcccagtc aaaaccttcc
       61 taaaacaacc ctttggcggc cttggagtat aatttaatct tcctttgatg actccagatt
      121 tcaccctgcc tcaggtcact cggagaacat caataattgc tgagtccagg tggagccggg
      181 tatgaaagat gatccattgc aggaatgctg cctcccatgt gcaggagcac ctctcttgct
      241 ggttgttctc cactccaggt cctctgaaca tcagctcaga ggcactctgg acacgcagac
      301 ctgagtcctt ttccagattc tcgcttagga aacatgccct gagccactaa gccctttgtc
      361 cagcaagcag cctctaggct cagccacact ccactcccca gccccctctc caccctatca
      421 acagccttgg ctgaggcagc cctttcagga gagctgggct tggtgggtgc acagcattcc
      481 ccctgccgga tgttaatctt ccaaagaaag tcaaacatta gcccaggaaa cagcttatgc
      541 cttatataag gcaccccagg ggagacacaa acagcttgtt agagctgagc tgtcctacta
      601 cagcagctgc tggaccctag gacagagcac gggcaactgc tgcatccagc tccagcccaa
      661 ctgaatccag ctccaaccac cagttttggt tcatcttgcc tggtgccatg accttctccg
      721 agattctgga ccgtgttgga agcatgggcc ccttccagta cctgcatgtg accttgctgg
      781 ccctcccaat cctcggaata gccaaccaca acttgctaca gatcttcaca gccaccaccc
      841 ctgaccacca ctgtcgcccg ccccccaacg cctctctaga gccctgggta ctccccttgg
      901 gcccaaacgg gaagcctgag aagtgtctcc gcttcgtgca tctgccaaac gccagtcttc
      961 ccaatgacac ccagggggcc accgagccat gcttggatgg ctggatctac aacagcacca
     1021 gagacaccat tgtgacagag tgggacttgg tatgcggctc caacaaactg aaggagatgg
     1081 cacagtcagt cttcatggca ggtatactgg ttggaggacc tgtgtttgga gaactgtcag
     1141 acaggtttgg ccgcaagccc atcctgacct ggagctatct cttgctggca gccagtggct
     1201 ccagtgctgc cttcagcccc agcctcactg tctatatgat cttccgattc ctgtgtggct
     1261 gcagcatctc gggcatttct ctgagcacca ttatcttgaa tgtggaatgg gtacccacct
     1321 ccacgcgggc catctcatca acaactattg ggtactgcta caccattggt caattcattc
     1381 tgcctggcct ggcctatgcc gttcctcagt ggcgctggct acagttgtcc gtgtctgctg
     1441 ccttcttcat cttctccttg ttgtcctggt gggtaccaga gtccatacgc tggctggttc
     1501 tgtctggaaa attctcaaag gctctgaaga cactccaacg tgtggctacc ttcaacggca
     1561 agaaggagga aggggaaaag ctcactgtgg aggagctgaa gttcaacttg cagaaggaca
     1621 tcacctcagc caaggtcaaa tatggcttat ctgacttgtt ccgagtgtcc atcctgcgcc
     1681 gtgtgacctt ctgtctctct ctggcctggt ttgctactgg ctttgcctac tacagtttgg
     1741 ctatgggagt agaagaattt ggagtcaaca tctacatact ccagatcatc ttcggtgggg
     1801 ttgacattcc cgccaagttc atcacaatcc tctccataag ttatctgggc cggcgcatca
     1861 ctcagggctt cctcctgatc ctggcaggag tggccatcct ggccctcatc tttgtgtctt
     1921 cagaaatgca gctcttgaga acagcactgg ctgtatttgg gaagggatgc ctgtctggct
     1981 ccttcagctg cctcttcctc tacacaagtg agctctaccc tacagtcctc aggcaaacag
     2041 gtatgggtat cagtaacata tgggctcgag tgggaagtat gatagcccca ctggtgaaaa
     2101 tcacgggaga actgcagccc ttcatcccta atgtcatctt tgggaccatg actctactgg
     2161 gaggcagtgc tgccttcttt ctgcttgaga ccctcaatcg gcccttacca gaaactatcg
     2221 aggacataca agactggtac cagcaaacca agaaaacaaa gcaggagcca gaagcagaaa
     2281 aggcatccca gacaatcccg ctgaagactg gtggacccta gctaagaaca acagaatcct
     2341 ctttcctgcc ttccagagac tgatcccaag cagggccctt ccaaggctat tcgagcacct
     2401 taggggttgg gtggagcccc agctggctcc atgctctcag aacaaagact tctgagagtt
     2461 cagcaaaagt gttttacctt caccacctcc accgtagccc acaacccaga cctggcctgt
     2521 tcacagccct agccatactc actcctgcac tcatcctccc tgcaacccag gccctgccat
     2581 ttttctctac cctctttgta ttggccattt cctccattgt cccacctcca tttccctttg
     2641 agattccctg gcagttctaa tggtttcctc ttaccttccc caaactctct ccttggtggg
     2701 aaatttcaat aaaccacaat gaagaactca ggccatgctt cgtgggaaag agggctggcc
     2761 atgatgtgga catgtgagca cacacacata ccacacacat acacatacac acacacacac
     2821 acacacacac acacacacac acacacacac acacttgtcc atctgtacca tatgaacata
     2881 aagatatggg aactaagctg catgcaagct agcctgctat gaatgcatac atgtgtccta
     2941 gtctgtttgt gcaggtataa caaactacca caagctggat tgtctgaata acagaactgt
     3001 gttgctcata gttgtagagg ctcggaagtc caacctgggt caagggatcg ggcattcagt
     3061 gactggtgag aaccttcata ctgtgtctct acaaagctgg ttctgtccag ctatttcatc
     3121 aggcactaat ccatttcctg agagcaaagc tgtgttagct ttctatcacc atgtcaaagt
     3181 acctgaggta aacaacttaa aacaaaagtt ttatctgggc tctcagttgg aggttttgtt
     3241 ccatgctcac taggcattgt tgcctcagag ccactgtaga acatcatcac gggtagcact
     3301 tcaaccctct ccaagggttt atattcccga ggcctgctaa tcaaatactt ccagaaccct
     3361 acatattagg gtttctattg ctatggtaaa acaccatgat taaaagcctc tccgggagaa
     3421 aagggtttat ttcatcttac aatgctcagg ttccacctcc tcaaatcagg gcaggaactg
     3481 aagtagaggt catgaaggaa cacggcttac tgactgctcc tcttggcacc accagcccag
     3541 gggtggcatc agccatagtg ggccaggtcc tgccacgtca atcattaatc atgccaatac
     3601 cccacaggtt tgtctacatg ccagtcttat ggagggattt ctcaattagg attccctcct
     3661 cctaacttgt gttacgtgac aaaaaaaaaa aaaaagagcc aggacactca gcttggatca
     3721 gttccatcca agcactcaac agaggctatt gccactatat aggacatgcc aacctactct
     3781 attgcttccc attatcaacc acagagctaa tgactgctct caagtatgta tttagagatt
     3841 tgagagggtg cttaataaac ataaaaggat tttactgta
//
