LOCUS       NM_009200               2089 bp    mRNA    linear   ROD 06-FEB-2024
DEFINITION  Mus musculus solute carrier family 1 (high affinity
            aspartate/glutamate transporter), member 6 (Slc1a6), mRNA.
ACCESSION   NM_009200
VERSION     NM_009200.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Malhotra,S., Banumurthy,G., Pennock,R.L., Vaden,J.H., Sugihara,I.,
            Overstreet-Wadiche,L. and Wadiche,J.I.
  TITLE     Climbing Fiber-Mediated Spillover Transmission to Interneurons Is
            Regulated by EAAT4
  JOURNAL   J Neurosci 41 (39), 8126-8133 (2021)
   PUBMED   34400517
  REMARK    GeneRIF: Climbing Fiber-Mediated Spillover Transmission to
            Interneurons Is Regulated by EAAT4.
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Miazek,A., Zalas,M., Skrzymowska,J., Bogin,B.A., Grzymajlo,K.,
            Goszczynski,T.M., Levine,Z.A., Morrow,J.S. and Stankewich,M.C.
  TITLE     Age-dependent ataxia and neurodegeneration caused by an alphaII
            spectrin mutation with impaired regulation of its calpain
            sensitivity
  JOURNAL   Sci Rep 11 (1), 7312 (2021)
   PUBMED   33790315
  REMARK    Erratum:[Sci Rep. 2021 Sep 8;11(1):18218. PMID: 34497286]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2089)
  AUTHORS   Perkins,E.M., Clarkson,Y.L., Suminaite,D., Lyndon,A.R., Tanaka,K.,
            Rothstein,J.D., Skehel,P.A., Wyllie,D.J.A. and Jackson,M.
  TITLE     Loss of cerebellar glutamate transporters EAAT4 and GLAST
            differentially affects the spontaneous firing pattern and survival
            of Purkinje cells
  JOURNAL   Hum Mol Genet 27 (15), 2614-2627 (2018)
   PUBMED   29741614
  REMARK    GeneRIF: EAAT4 role in the spontaneous firing pattern and survival
            of Purkinje cells.
REFERENCE   4  (bases 1 to 2089)
  AUTHORS   Rabenstein,M., Peter,F., Rolfs,A. and Frech,M.J.
  TITLE     Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell
            Firing Pattern of NPC1-deficient Mice
  JOURNAL   Sci Rep 8 (1), 3318 (2018)
   PUBMED   29463856
  REMARK    GeneRIF: Reduced Cerebellar EAAT4 Expression associated with loss
            of cerebellar Purkinje cells resluts in neurodegeneration and
            Niemann-Pick disease Type C1.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2089)
  AUTHORS   Zhou,L., Yang,L., Li,Y.J., Mei,R., Yu,H.L., Gong,Y., Du,M.Y. and
            Wang,F.
  TITLE     MicroRNA-128 Protects Dopamine Neurons from Apoptosis and
            Upregulates the Expression of Excitatory Amino Acid Transporter 4
            in Parkinson's Disease by Binding to AXIN1
  JOURNAL   Cell Physiol Biochem 51 (5), 2275-2289 (2018)
   PUBMED   30537735
  REMARK    GeneRIF: Overexpressed miR-128 downregulates the expression of
            AXIN1 but upregulates that of EAAT4 in dopamine neurons of
            Parkinson's disease mice.
REFERENCE   6  (bases 1 to 2089)
  AUTHORS   Hu,W.H., Walters,W.M., Xia,X.M., Karmally,S.A. and Bethea,J.R.
  TITLE     Neuronal glutamate transporter EAAT4 is expressed in astrocytes
  JOURNAL   Glia 44 (1), 13-25 (2003)
   PUBMED   12951653
  REMARK    GeneRIF: These results demonstrate that EAAT4 is expressed in
            astrocytes. This astrocytic localization of neuronal EAAT4 may
            reveal a new function of EAAT4 in the central nervous system.
REFERENCE   7  (bases 1 to 2089)
  AUTHORS   Surendran,S., Rady,P.L., Michals-Matalon,K., Quast,M.J.,
            Rassin,D.K., Campbell,G.A., Ezell,E.L., Wei,J., Tyring,S.K.,
            Szucs,S. and Matalon,R.
  TITLE     Expression of glutamate transporter, GABRA6, serine proteinase
            inhibitor 2 and low levels of glutamate and GABA in the brain of
            knock-out mouse for Canavan disease
  JOURNAL   Brain Res Bull 61 (4), 427-435 (2003)
   PUBMED   12909286
  REMARK    GeneRIF: abnormal expression of EAAT4, GABRA6, Spi2 combined with
            lower levels of glutamate and GABA are likely to be associated with
            the pathophysiology of Canavan disease.
REFERENCE   8  (bases 1 to 2089)
  AUTHORS   Wiltshire,T., Pletcher,M., Cole,S.E., Villanueva,M., Birren,B.,
            Lehoczky,J., Dewar,K. and Reeves,R.H.
  TITLE     Perfect conserved linkage across the entire mouse chromosome 10
            region homologous to human chromosome 21
  JOURNAL   Genome Res 9 (12), 1214-1222 (1999)
   PUBMED   10613844
REFERENCE   9  (bases 1 to 2089)
  AUTHORS   Maeno-Hikichi,Y., Tanaka,K., Shibata,T., Watanabe,M., Inoue,Y.,
            Mukainaka,Y. and Wada,K.
  TITLE     Structure and functional expression of the cloned mouse neuronal
            high-affinity glutamate transporter
  JOURNAL   Brain Res Mol Brain Res 48 (1), 176-180 (1997)
   PUBMED   9379843
REFERENCE   10 (bases 1 to 2089)
  AUTHORS   Yamada,K., Wada,S., Watanabe,M., Tanaka,K., Wada,K. and Inoue,Y.
  TITLE     Changes in expression and distribution of the glutamate transporter
            EAAT4 in developing mouse Purkinje cells
  JOURNAL   Neurosci Res 27 (3), 191-198 (1997)
   PUBMED   9129177
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK159197.1, BC138120.1, BB668161.1 and BB807884.1.
            
            [WARNING] On Mar 30, 2024 this sequence was replaced by
            NM_009200.4.
            
            On Mar 27, 2010 this sequence version replaced NM_009200.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159197.1, D83262.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1010              AK159197.1         2-1011
            1011-1946           BC138120.1         913-1848
            1947-2029           BB668161.1         330-412
            2030-2089           BB807884.1         338-397
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2089
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /note="solute carrier family 1 (high affinity
                     aspartate/glutamate transporter), member 6"
                     /db_xref="GeneID:20513"
                     /db_xref="MGI:MGI:1096331"
     exon            1..186
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            187..389
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..1879
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /note="solute carrier family 1, member 6; high-affinity
                     neuronal glutamate transporter; sodium-dependent
                     glutamate/aspartate transporter"
                     /codon_start=1
                     /product="excitatory amino acid transporter 4"
                     /protein_id="NP_033226.1"
                     /db_xref="CCDS:CCDS23970.1"
                     /db_xref="GeneID:20513"
                     /db_xref="MGI:MGI:1096331"
                     /translation="MSSHGNSLFLRESGAGGGCLQGLQDSLQQRALRTRLRLQTMTRE
                     HVRRFLRRNAFILLTVSAVIIGVSLAFALRPYQLSYRQIKYFSFPGELLMRMLQMLVL
                     PLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTVIAVFIGILMVTIIHPGKGSKEGL
                     HREGRIETVPTADAFMDLVRNMFPPNLVEACFKQFKTQYSTRVVTRTIVRTDNGSELG
                     ASMSPTSSVENETSILENVTRALGTLQEVISFEETVPVPGSANGINALGLVVFSVAFG
                     LVIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGG
                     QLGMYTLTVIVGLFLHAGGVLPLIYFLVTHRNPFPFIGGMLQALITAMGTSSSSATLP
                     ITFRCLEEGLGVDRRITRFVLPVGATVNMDGTALYEALAAIFIAQVNNYELNLGQITT
                     ISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDITLIIAVDWFLDRLRTMTNVLG
                     DSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGNESVM"
     exon            390..527
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            528..732
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            733..775
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            776..1119
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1353
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1548
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1683
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..2089
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gattgcatag cgcatccctg ttccgcgtcg ctctctctgt cttcgggtcc ccaacgcaga
       61 cacagcgcgg gtccctggag ggtccctgcg gacccggcca ggtccttcag cgggttctgg
      121 cttctgcatc ccggcgcagc gcgcaggcca aggcgcgggc aaggcggccc cgctgacccg
      181 aggctgagac gcgatgagca gccacggcaa tagtctgttc ctgagggaga gcggtgccgg
      241 tgggggctgt ctgcagggcc tgcaggacag cctgcagcag agagcactgc gcacgcgctt
      301 gcgcctgcag accatgaccc gagagcacgt gaggcgcttc ctgcgccgga atgccttcat
      361 cttgctcaca gtcagcgctg tgatcattgg tgtcagcttg gcatttgcct tgcgcccgta
      421 ccagctctcc taccgccaga tcaagtactt ctcctttcct ggtgagctgc tcatgaggat
      481 gctgcagatg ctggtgttac ccctcattgt ctccagcctt gtcacaggta tggcatccct
      541 ggacaacaag gcgacagggc ggatgggaat gcgggcagct gtgtactaca tggtgactac
      601 agtcattgca gttttcatcg gcatcctaat ggttaccatc atacatcctg ggaagggctc
      661 caaggagggg ctgcaccgtg agggccgaat tgagactgtc ccaacagctg atgctttcat
      721 ggacctggtc agaaatatgt ttccacctaa ccttgtggag gcctgcttca aacagtttaa
      781 gacacagtac agcacacgag tggtgacaag gacaattgta aggacagaca atggttcaga
      841 gctgggggcc tccatgtctc ccacatcctc agtagaaaat gaaaccagca tcctggaaaa
      901 tgtcacccgg gccttgggca ccctgcagga ggtgataagc tttgaggaga ctgtgcctgt
      961 acctggctca gccaatggca tcaatgcctt gggccttgtg gtcttctctg tggcctttgg
     1021 gctggtcatt ggtggcatga agcacaaagg ccgtgtcctg agggacttct ttgacagcct
     1081 caatgaggct attatgaggc tggtgggcat catcatctgg tatgcaccag tgggcatcct
     1141 gttcctgatt gctggaaaga ttctggaaat ggaagacatg gctgtccttg gaggtcagct
     1201 gggcatgtac acactgactg tcatcgttgg cttattcctt catgctggtg gtgtgttgcc
     1261 ccttatctac ttcctcgtca cccatcggaa cccctttccc ttcattggtg gtatgctaca
     1321 ggcactcatc acagctatgg gtacctcttc cagctctgca actctgccta tcactttccg
     1381 ctgcctggag gagggcctgg gtgtggatcg tcgcatcacc aggttcgtgc tgcctgtggg
     1441 ggccactgtc aacatggatg gcactgcact ctacgaggcc ttggcagcca tctttattgc
     1501 tcaagtcaac aactatgagc tcaaccttgg tcagatcacc acaatcagca tcacagccac
     1561 agctgccagc gtaggagcag ctggcatccc ccaggcagga ctggttacca tggtgattgt
     1621 gctcacatcc gtcggcctgc ccacagagga catcacactg atcatagctg tggattggtt
     1681 ccttgaccga cttcgtacaa tgaccaatgt actgggggat tcgattggag cggctgtcat
     1741 tgagcatttg tcccaacggg agctggagct gcaggaggct gagctgactc tacccagcct
     1801 ggggaaaccc tataagtcac tcatggcaca agaaaagggg gcatcaaggg gtcggggagg
     1861 taatgagagc gtcatgtgag aagcttctag ctctttgtcc agggcggagg gaaggggctg
     1921 ggctgggagt cctggggaca atcggttgcc caactgactg tgggctgaac atacatgttc
     1981 tgtgtgggtt ctctggggac tgccgagata aacaagagga atgaaaggta tccaacgtgt
     2041 gttcatcact gttgcatttg tgtgctcgaa atataagaac aacctaagg
//
