LOCUS       NM_001077514           11565 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus solute carrier family 1 (glial high affinity glutamate
            transporter), member 2 (Slc1a2), transcript variant 1, mRNA.
ACCESSION   NM_001077514
VERSION     NM_001077514.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Li,S., Wang,J., Andersen,J.V., Aldana,B.I., Zhang,B.,
            Prochownik,E.V. and Rosenberg,P.A.
  TITLE     Misprogramming of glucose metabolism impairs recovery of
            hippocampal slices from neuronal GLT-1 knockout mice and
            contributes to excitotoxic injury through mitochondrial superoxide
            production
  JOURNAL   J Neurochem 169 (1), e16205 (2025)
   PUBMED   39193789
REFERENCE   2  (bases 1 to 11565)
  AUTHORS   Chongtham,A., Sharma,A., Nath,B., Murtha,K., Gorbachev,K.,
            Ramakrishnan,A., Schmidt,E.F., Shen,L. and Pereira,A.C.
  TITLE     Common and divergent pathways in early stages of glutamate and
            tau-mediated toxicities in neurodegeneration
  JOURNAL   Exp Neurol 382, 114967 (2024)
   PUBMED   39326823
REFERENCE   3  (bases 1 to 11565)
  AUTHORS   Ratsika,A., Codagnone,M.G., Bastiaanssen,T.F.S., Hoffmann
            Sarda,F.A., Lynch,C.M.K., Ventura-Silva,A.P., Rosell-Cardona,C.,
            Caputi,V., Stanton,C., Fulling,C., Clarke,G. and Cryan,J.F.
  TITLE     Maternal high-fat diet-induced microbiota changes are associated
            with alterations in embryonic brain metabolites and adolescent
            behaviour
  JOURNAL   Brain Behav Immun 121, 317-330 (2024)
   PUBMED   39032541
REFERENCE   4  (bases 1 to 11565)
  AUTHORS   Gan,Z., Guo,Y., Zhao,M., Ye,Y., Liao,Y., Liu,B., Yin,J., Zhou,X.,
            Yan,Y., Yin,Y. and Ren,W.
  TITLE     Excitatory amino acid transporter supports inflammatory macrophage
            responses
  JOURNAL   Sci Bull (Beijing) 69 (15), 2405-2419 (2024)
   PUBMED   38614854
REFERENCE   5  (bases 1 to 11565)
  AUTHORS   Tan,R.L., Sciandra,F., Hubner,W., Bozzi,M., Reimann,J., Schoch,S.,
            Brancaccio,A. and Blaess,S.
  TITLE     The missense mutation C667F in murine beta-dystroglycan causes
            embryonic lethality, myopathy and blood-brain barrier
            destabilization
  JOURNAL   Dis Model Mech 17 (6) (2024)
   PUBMED   38616731
REFERENCE   6  (bases 1 to 11565)
  AUTHORS   Sutherland,M.L., Delaney,T.A. and Noebels,J.L.
  TITLE     Glutamate transporter mRNA expression in proliferative zones of the
            developing and adult murine CNS
  JOURNAL   J Neurosci 16 (7), 2191-2207 (1996)
   PUBMED   8601800
REFERENCE   7  (bases 1 to 11565)
  AUTHORS   Sutherland,M.L., Delaney,T.A. and Noebels,J.L.
  TITLE     Molecular characterization of a high-affinity mouse glutamate
            transporter
  JOURNAL   Gene 162 (2), 271-274 (1995)
   PUBMED   7557442
REFERENCE   8  (bases 1 to 11565)
  AUTHORS   Mukainaka,Y., Tanaka,K., Hagiwara,T. and Wada,K.
  TITLE     Molecular cloning of two glutamate transporter subtypes from mouse
            brain
  JOURNAL   Biochim Biophys Acta 1244 (1), 233-237 (1995)
   PUBMED   7766664
REFERENCE   9  (bases 1 to 11565)
  AUTHORS   Li,X. and Francke,U.
  TITLE     Assignment of the gene SLC1A2 coding for the human glutamate
            transporter EAAT2 to human chromosome 11 bands p13-p12
  JOURNAL   Cytogenet Cell Genet 71 (3), 212-213 (1995)
   PUBMED   7587378
REFERENCE   10 (bases 1 to 11565)
  AUTHORS   Kirschner,M.A., Copeland,N.G., Gilbert,D.J., Jenkins,N.A. and
            Amara,S.G.
  TITLE     Mouse excitatory amino acid transporter EAAT2: isolation,
            characterization, and proximity to neuroexcitability loci on mouse
            chromosome 2
  JOURNAL   Genomics 24 (2), 218-224 (1994)
   PUBMED   7698742
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK134609.1 and AL844155.6.
            
            On Feb 25, 2018 this sequence version replaced NM_001077514.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140095.1, AK134609.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2367              AK134609.1         8-2374
            2368-11565          AL844155.6         19889-29086
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 54.13 cM"
     gene            1..11565
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /note="solute carrier family 1 (glial high affinity
                     glutamate transporter), member 2"
                     /db_xref="GeneID:20511"
                     /db_xref="MGI:MGI:101931"
     exon            1..613
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             597..2315
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     solute carrier family 1, member 2; excitatory amino acid
                     transporter 2; sodium-dependent glutamate/aspartate
                     transporter 2; glutamate transporter 1c; EAAT2 variant
                     mEAAT2/5UT5; EAAT2 variant mEAAT2/5UT2"
                     /codon_start=1
                     /product="excitatory amino acid transporter 2 isoform 1"
                     /protein_id="NP_001070982.1"
                     /db_xref="CCDS:CCDS38188.1"
                     /db_xref="GeneID:20511"
                     /db_xref="MGI:MGI:101931"
                     /translation="MASTEGANNMPKQVEVRMHDSHLSSDEPKHRNLGMRMCDKLGKN
                     LLLSLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLI
                     TGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKND
                     EVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPSEEANTTKAVISMLNETM
                     NEAPEETKIVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGEQAKLMVEFFNILNEIV
                     MKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLI
                     YFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVG
                     ATINMDGTALYEAVAAIFIAQMNGVILDGGQIVTVSLTATLASIGAASIPSAGLVTML
                     LILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHR
                     MQEDIEMTKTQSIYDDKNHRESNSNQCVYAAHNSVVIDECKVTLAANGKSADCSVEEE
                     PWKREK"
     exon            614..753
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            754..906
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1157
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1323
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1450
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1684
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1879
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2014
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2243
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..11565
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11539..11544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /note="hexamer: AATAAA"
     polyA_site      11560
                     /gene="Slc1a2"
                     /gene_synonym="1700091C19Rik; 2900019G14Rik; Eaat2; GLT-1;
                     GLT1; MGLT1"
                     /note="major polyA site"
ORIGIN      
        1 gatgtcagct ctggacaaaa atagagaggg atcgcctgca aatccccagc tccggctggg
       61 ctaaaccttg caatccctct tcggccggcg ccgagccagg gcgcagcggc ctctaccgcc
      121 tcccccggcg cgcacacacc agcacacgcg cacgcacgca cacactcccc cgcactcgca
      181 ctcccggtct tccccatccc gctcggtgcc ggccactcct ggtaccggtc tgccccgcgg
      241 cgaagcacac agacacgcac acacacaggt ggctgctggg aagcggggag tgtgttcctg
      301 cagccctgcg gccagcgcga cctcgcagca gccggtgcag gatccccgca gcgcccctcg
      361 gagcgctggc cgcgagccgc tcacgggacc gcgcgcggtg gaaagccggg acgtggatta
      421 gcgcccgcag cagcctcccg cgcgcgccga ggcgctaaag ggcttaccca ggaggggggt
      481 gcgagggcgg agagaggctg cccgttaaat accgctctcc gccgcactcc gggctcaccc
      541 agctcgccgc caccgtctcc agaccgtgcc cgggaggagg gggcgttccc cacgccatgg
      601 catcaacaga gggtgccaac aatatgccca agcaggtaga agtgcgcatg catgacagcc
      661 acctcagctc cgatgagcca aagcaccgaa acctgggcat gcgcatgtgc gacaagctgg
      721 ggaaaaatct cctgctctca ctgactgtgt ttggtgtcat cctgggagca gtgtgtggcg
      781 ggctgcttcg cttggcatcg cccatccacc ctgatgtggt catgttgata gccttcccgg
      841 gggacatact catgaggatg ctgaagatgc tcatcctccc tcttatcatc tccagtttaa
      901 tcacagggtt gtcaggcctg gatgctaaag ccagcggccg cctaggcacg agagctatgg
      961 tgtattacat gtccacgacc atcattgccg ccgtgctggg ggtcatcctg gtgttggcca
     1021 tccacccagg caatcccaaa ctcaagaagc agctagggcc cgggaagaag aacgacgagg
     1081 tgtctagcct ggatgccttc ctggatctca ttagaaatct cttcccggag aacctggtgc
     1141 aagcctgttt ccagcagatt cagacagtga caaagaaagt tctggtggca cctccatctg
     1201 aggaggccaa taccaccaag gcggtcatct ccatgttgaa tgaaaccatg aacgaggccc
     1261 ctgaagaaac taagatcgtt atcaagaagg gcctggagtt caaggacggg atgaacgtct
     1321 taggtctgat cggattcttt attgctttcg gcattgccat ggggaagatg ggtgaacagg
     1381 ccaagctgat ggtggagttc ttcaacattc tgaatgagat cgtgatgaag ttagtgatca
     1441 tgatcatgtg gtactcccct ctgggtatcg cctgcttgat ttgtgggaag atcatcgcca
     1501 tcaaggactt agaagtggtt gctaggcagc tggggatgta catgatcacc gtgatcgtgg
     1561 gcctcatcat tcacgggggc atctttctcc ccttgattta ctttgtagtg accagaaaaa
     1621 atccattctc cttttttgct ggcatattcc aagcctggat cactgctctg ggaactgctt
     1681 ccagtgctgg aactttgcct gttaccttcc gttgcttgga agataatcta gggattgaca
     1741 agcgtgtgac cagattcgtc ctcccagtcg gagcaaccat taacatggat ggcacagccc
     1801 tttacgaggc tgtggcagcc atcttcatag cccaaatgaa tggggtcatc ttggatggag
     1861 gtcagattgt gactgtaagc cttacagcca ccctggcgag cattggtgca gccagtattc
     1921 ccagcgccgg gctggtcacc atgctcctca ttctcacagc tgtgggcctg ccaacggagg
     1981 atatcagtct gctggtggcg gtggactggc tgctggatag aatgagaact tcagtcaatg
     2041 tggtgggcga ttcttttggg gctgggattg tctatcacct ttccaagtct gagctggaca
     2101 ccattgactc ccaacaccga atgcaggaag acatcgaaat gaccaagacg cagtccattt
     2161 acgacgacaa gaaccacagg gaaagcaact ctaatcagtg tgtctatgcc gcacacaact
     2221 ctgtcgtaat agatgagtgc aaggtaactc tggcggccaa tggaaagtca gctgactgca
     2281 gtgttgagga agaaccttgg aaacgtgaaa aataatgacc cgattctcag ccaattcttg
     2341 aataaactcc ccagcgtatc ttatggtaaa agatgctctc taaacaggct tcctttacaa
     2401 aaggaaaaga tgcatatgtt tctatgttta cttaatctgt tagctgaggc atagaggagc
     2461 tgtgtacacc cgtgatgata ggcaccgtgc tgtgtctttg ccaaataatg ctttataact
     2521 gtctaatttt cttacttgtg ttattgtttg aatggacgct gctggagaaa ctactttgaa
     2581 tggaagacgt gttcttgcca gcttcctttt gtctcagtgc aacactgtgg atgctatttt
     2641 ttttccaagg ggacaggatg aaagcagtgt ggtaccctcc agggacttaa gacaacgagc
     2701 acacatgtgc tgtcttcctt aaagtgttca tggctcacct tgttctacac aaaagattct
     2761 gcttgaagct tctagaatta cctcccctcc agcctgtaga gagttggcac ctacattgat
     2821 tgaaagcatc tgcctggggg aactctgaca acttaccttt ttgtgcactg tctaagtaga
     2881 atgtttcaga gtttcatggg tgcccatact ctgaaatgca ggtgaccata aaggccacag
     2941 ctctgccttt ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgagaga
     3001 gagagagaga gagagagaga gagagagaga gagagagatg tgtgtgtgtg aaaaatgtga
     3061 aatttcaaat ttgaaaatga tctaggaatc atttgcatat taccactgta taccttgagg
     3121 aaagtgaagg agcatagtca acttgaattt tagtcagaat taaatattcc aattctcttg
     3181 tttgacttgc attcgtagag catattgaac tcaagtgtga tgatctgatc tggttttttg
     3241 ttccacccat agattctcac ttgagttcct caccgaaaga tatttctaac acccaagtca
     3301 cttccatcac atccttgtct gcttatatgg ctgagcactt cagcttcctc acattaagag
     3361 acacacgttg gctatgactc ttttcctatc atcctggtat atatctaata tgtagaaagt
     3421 gtttgttata ttaaagcaca tgaaaaatat cacaaaacac aaaagttctt tggtgagaag
     3481 ggaacagaaa ccacacatgt atataaaatc ctataaaggt tcctatgaca gaggccaggc
     3541 agcctcaaac catcagatgg ggaccaagaa cggctctaca caactttcca gatagaatcc
     3601 tttccgggga ctgtgaaagt ctgaaggaat aaataatata attggcacag catgagggtt
     3661 tgcctgtggt aggcagccaa cgaatcctta atgatgggtt gtgcaagcta aaattgtgga
     3721 tgtctctatt attatcttct gttgaagaat gcacagtatt ttcctaagat cctgggttta
     3781 acggttagcg gggaactgga accactgaaa aacaaaacaa attcaacttg aaggagtgtc
     3841 ggaacccaag ccatcagtca gggcatgtgt gtcctctgaa gcaggcagag ctgaggagac
     3901 gttcataagc agtaatagga cccattgcct ctgaagaagg gcacggggcc atcgtttctc
     3961 ctcttagccc ctcttgtgac ttctctcctt caaatcagac agctcagaca catacaaaag
     4021 acaagagatt cctgttgctt tatctcatga accctaactg tttttaaaca aggatcacta
     4081 ttgagcagta ttaaatgaat tagtttgttc tgtttcaggt tttgactttt gaaacaaggt
     4141 ctttttatgt agcccagaat ggcttcaaac tctcacacct actgctcagc ctcggttcta
     4201 agatcacagg tgggcaccac tgcatcggct ggcagaactc ttgtttatac ttttctgaga
     4261 gacagtgaac agggtagcag tgaccagctg tgaaaccctg aaggaagaca agaagggcca
     4321 aagggattct gctcagctgt ggaactattc tagatagcct gtaagtggta ccccagggct
     4381 ctcctatgct gagtagatgt atctaggcaa ggcatcagga gcatgctggg ttttgctgtg
     4441 ttggggaggg aaaggccatt ccatacaatg agtctcccca tacaagaaga aacaaggcca
     4501 cgtgggtact tgaacattgc tcttttctta aaagcactca caggatgaca gtgtttgtca
     4561 tctcagattc tgcctgaaaa tctttctaat ttgttttatc ttcaccaccc cccccccagc
     4621 agtcctgtta gcttatgtaa agaagacaga gctttcagga gaacaaaggc acattatttc
     4681 tcaagtcagg ttttgctgac tacatatgct gtctcaagag aatatgctag ggaaatcaca
     4741 tttactgaaa atttaataat ttttatttaa aataaataat ttatcaaatt ggagacttcc
     4801 ctaagaaatc tagaatgtga tggaattgac atagtactgt gtcttgcttt tgtaagcctt
     4861 gttaaccctt tccatgccca ttggagattc ccttgcctag aaaaggtgca ggaagctgaa
     4921 gaaaaaaaaa acagatacag agtgtgttct agtagcctta gtctaaatag ggtacctgtc
     4981 accgtgtgtg tattaataga gccatcgtag atctgaatgt ccataatgat gattaatctt
     5041 ttatgtgtag ttctctctct gcctctgtct ctgtctctcc ctttcatatg cacacacaca
     5101 cacacacaca cacacacaca cacacacaca cacacacaca gaacatgtgt gtcacataca
     5161 ctaactccag gaaccaggga ggtttgtgta ttggggcttt ggcagtgcta ttcctgtaac
     5221 ggattcagtt aatacttaca aagaatcaaa atcatttcag aaaaaacaaa caagtgagca
     5281 gacgttcatc ttccctgtct cgtcctttcc ctataagaag taagacaaat catcatttta
     5341 ttaactgctg agtaaaactc aacaaagtca attcagccag tgaacagcat ggttgtttcg
     5401 aaggtaaaat ttcactgtgg gcttctgagg gtggcgggac accagagaat ccaggcagcc
     5461 atcaggacct aagcacacaa aacctcagta aaggcctgta ggtgatttta ggaccatgag
     5521 atcactactg atttggtttt tgagcttttt tttggggggg gggtggtaat aaaagcctgt
     5581 gtggcctcat tgcctgtgga aagttttatg ttctataaaa gacagctagc cttttctcct
     5641 cactagtcac tagttagttc ggacatttac agagcaaagg aaggactcca gaatgtctcc
     5701 agatgaaaac gaagaaagca cggaactgtg tatgaggaat tcagtttatg gatcagtccc
     5761 tgctggggga tgctgggatc tcagtggttc gctgattagg gtcaactatg gctagaaaat
     5821 catggcacat agaagtgcac gtagtcagac acccttcaga gagttgaaag gcctcagaag
     5881 aaaacccaac cctgccttgc tgctgtgagt aatcttccct ttgttctccg aagtaaaatc
     5941 ttattataat gttcactttt gtcagggtct aggctcttgc ctatgcatta ttttcctcat
     6001 gcaacaaagc aataaaccca agtaggtatt cgcatgctca ggctccgata aacaagccgg
     6061 agcagctcag gcctcctttg atgactaaaa tggcagtggt tacaaccccg gggcaacctg
     6121 cccggtttgc tgctctcttt aaggaccaat ctgtctgccc tgcttttttt tttttttctt
     6181 ttctgcttat tctgcagctg aactcatgat ctatcaggca ggacgaagga ccagatggga
     6241 ttttctagta ctgggatact ttcagggctt cagggtccaa gggtcttaag gagataaggt
     6301 tagcgtctga agcaagggtt tcctaaacca agttgtcact tgaagattca tggtcaccgc
     6361 atctacagta gtctctggtt aaacccaaaa gaaggattac tgagtgccaa cccagccagg
     6421 tggcaactgt gacagctaat tttccgtggg tggagtgaga taaagtctca tcaagaacac
     6481 caaccagctt aggattttcc ctaaaactct cttcccagtt cactgccctc gcccttccct
     6541 ttcctttcgt ttcattgctt aagagactgc ttagtaagtg gccaaatagt taagaggggg
     6601 ttggcttgga agcagataac aggagatctt aactatcagt aatagccagt ctcaccgcct
     6661 cacaccataa tcaggcaatg acacagagat gtttaaaaac aacgtggctt gggagaggaa
     6721 acatttgatt aagacactcc ccatctgtag ctgtaaggct cacacagcca ctgtgtgccc
     6781 aggtttcttg cacttccttc tgaaatgaga aggaactgga ccaactgcag ttttctaatc
     6841 tccttccatc ttactgttct caaacatgac ttatttatat tgtaagtttg aaagacacac
     6901 aaaatgaata ttcaatttag gtgtttcctt gtccatgatg agaaaactcc aactcaagct
     6961 tccattcatt ccatacttta caagcttgga gagtggccac agtaacccag tttccagcat
     7021 tcagacatca gtaaatatcc tttggttaaa atccctattc cccaaactca ctgttctcac
     7081 ggaagtggtg attttgctca gggaaatgtt ctgcaacatc accgtgaggg accagacacc
     7141 tggagtctga attttgagat cttgcatcat acctgctgta gcaacagagc ccactccaaa
     7201 ttctcagccc acatcatttt ccccaaacag tcctaattgc caaccctgtg tgggaatttg
     7261 ggagcctctg gcaaacaagc ttgaacactc ctgacatttg gtgcaagatt tacacaacag
     7321 caactgtctc cacacacctt tcccgccaag tacatgcaga cgtatacatc cattctgcgc
     7381 cttcccccac ccccgccccc acccccgccc cctggagaga ggatttgatt gatttcatta
     7441 gaaagttctc ctcttgattt acatgcataa cagacagcac atcattggga gagatgctgt
     7501 cacttataag aacggagttt tcccatttgc cccatgtccg tccatgttca cagagtgtct
     7561 aagtcattac agcagagagg gtggctgcac ctaaccttcc ctggttctgt ataaaaggtc
     7621 atagcttgca ttttttgctc tctgaccaaa tttcaaccag agttaaatgg atgtatttag
     7681 tgtggtgaac atcttcattg ccagaaaaga tttcagctct tcaaatttga gggagtatag
     7741 ccagttggga aagggctaat tattgttagt ggtgatggtg gttgggtttt tttctcctct
     7801 caaggtgaca gaagatctat ggtgagagca gaaagatgtt tcttaattga cctacttaag
     7861 gtcactgcag aaaaggggga gaagtccaga aggtcctggg agattgggtt cacactaaca
     7921 ctctgaatca tttctgatca gttttttttt ttttttctat gctgaatctt gtcttatcct
     7981 atgatccagt taccagtttt ggtattttta agtctttctc attgtatttt ggggaatttt
     8041 taggctgaga ataaaatcta ctattggaac agacctgtgc tctgcagagg catcacgtga
     8101 cccagacaac gggtggcaga atatctgggt tcccctggag acacccccaa cccctgggtg
     8161 agctttagaa cctaacaaca gactgttatc cttatcacca tcctctggga catgggggat
     8221 gatgcagtca gagccttgct acttctgggc tttgtttctc tctatggcaa ccattttatc
     8281 ctgcctggaa atggcttcct gccctacgtg gagagcaggc acacttacag ccaaggaagt
     8341 ggtcaaacag atccctaagt tctctccatg ctacaacaaa ttccctaata cacacaacca
     8401 gaaactcagc tcctgggtgg cttagtaccc acaaggtcac tttcctgttc taagagcctt
     8461 cggtatccag attcttctac aaaccacctc aactctttcc tctttcttcc ctcctccata
     8521 aaatctcagc atcccacccc cacattgcac aacagtgatc agatcagctg gtcactatag
     8581 agatttgtat atattaaaac aaacctactt tttggagaat tttttgtata ttcttttcta
     8641 tttgttttct acaaattgag gacagagctg gcaaactgtg aatctaattt tatcttgatg
     8701 ccagcagata tattttagct tcctggtatg aatctatgtt actagggaca ccatatcacc
     8761 ctctgatcag tattgcacct ccaatctaca aagttagttt ggcccaatct tacattctct
     8821 cttccacaaa tccccaaatt tctttgctat tatttataca gagaatattt atagtcctgg
     8881 atattacaag ttttaatgtt aatttaactt ttaataacat tacacatttg tatctactct
     8941 tcaaaaatca taaagactca gagtaggctg atatagtctc tttggtatct gtaatttttt
     9001 cagacagata ttttcttatg atattttcta taaaaccaca tagatagaag tatagctaat
     9061 tagagcacac atttctatag caatttgggg tatcaactgt ttcctgagca tacagacaca
     9121 tttgagaaat tttggaactc agtccttaat gatgcaatga ataacttgac caaactcaga
     9181 acgaagaatt gcttaagccc cttaggtagt tagcatatac aactgaacag ttaatccttc
     9241 agcatcaagg aatggtgctg actgctgttt cccgacacca gttcttgttt gcccaatgga
     9301 cctttctaat tttatttttc tataagaaga ataaaggccg gtgctaatag cgggaccgct
     9361 tctctatcag aatctcccat gcatttctag atgtttatgt gaaaattggc cctaatgaaa
     9421 cagagtagca aagactgagg atggttagga agaaaagaat tcaggacagg tttggtgcag
     9481 ttttaagtct tgccaagcaa aatgatagca aaacctcaca ttgttatatg aattgaacat
     9541 atatttttaa gaagaaatga aagacaacat atttttagta cccattcaga atccatgctg
     9601 tatccctggg ggcggggcag agctgggagt tagaggcata ctgagattat ttagcataaa
     9661 ctcaaatact ttatcctcag gtaggcaaca aacttatgta accttcccac aaggtaaacc
     9721 agtagccacg gaaaacacaa ggtgtctttt ctccaccgaa cacacactct gcaatggtgg
     9781 gaggactggt attttactgt gtttgaaaga atagcctgtt acatagatga catagtagga
     9841 tgtattcaaa cccgctcctc attaagcgaa cagattagct gtgccctcaa ctggctacga
     9901 ttgtatctgt tttcaccgct agctatcaga gcatgagttt cttgtgtgta cacgctgata
     9961 cttggttcac gcatgaagac agtgcctatg cactttgtgt agctatcatg taggtatttg
    10021 tgtgtaattt cagtgaaaca atgtatagac gtaatttaat gtaactgttt ttcttggctg
    10081 tttatctaaa tgcagtagat atgtcggttg ccgtttggaa accttccttt gcagtcttag
    10141 gtgtcgtcta agagttattt ctcgttctca atgtgacctg agtgaagaac ttaacctatg
    10201 aaacccctgt actcatgatt gtttgcaaat ctctgagtcc agcacctttg taaatacagc
    10261 cccgaggagg aatgggggtg aatgtgtttc ctgttactca gtgaaatatc agcctacgcc
    10321 tgcatcattc ccactcagtg caccagtgga acttgtggtt accaagtctg agggaggttt
    10381 agaatgaaga cactcgtctc tcctccctca ctggtggact gggcttaaag agacagaaaa
    10441 aaaaatcaaa aagtccatct cctcttcata ctcaaatcat gtcttctggg ggaagggtca
    10501 acctctctgg ttgtcagatc ttcacttgat ggtgaccttc atcatggcct aaacatcatg
    10561 gcctgaagaa ccttgttacc tttgacttcc cgagttggag tacaagaatg tttttattga
    10621 ttagtagtca tgcaggtgag cctgagcctc ccccgaaagc acagttctgg cagcaaattg
    10681 cacacctgtg agagaacaac agagtactag aagcacacga aggctcaggg ccagcaacag
    10741 ccaggggcca gcaatggttg aggaccagca aaggccagtg gtcaaccttg gcattggcag
    10801 aggaccaacc tttgatggac tgatcttgag tatgtgacag gaggctgatc tcctctactc
    10861 agcctgctct gcttgcctgt gtggagtagg tggagatgag attaacaatg gcaattagat
    10921 gtgattttgc gaactgcatt ctcactaaat accatacttt gtagttcctg taacccctct
    10981 cttccccaca aagacagaag aaacagcaat gtgctcgaga gtacccctcc ctttcctcac
    11041 agatcattga gaggtgaagc aagcacactc attttcaggg gcttccccag atgtgtggag
    11101 tgaggggata tggtgactgt gcccttcaaa ttatggatga aaaaaaaaaa agtacagaag
    11161 ggaaggagac tttaggcagg attatttttg aatgaaagat cagattaatg ttttcccttc
    11221 cgctcacagt ccccgttggt ctggtgtcat gtagcatagt gcgtctatag cacatggtat
    11281 ttaaagacta atgaatgcaa agagactaat ttgggggctg tttcttatat atggtactag
    11341 gaaatgcctt gttggcaaag cacaccttgg gtagttgaga tctcctgtgt tcgtctttag
    11401 ctttccttgt gatgtagact catgactgta acatttcatg tgtaaaataa ctggatattc
    11461 ttcatatgat ggaaataacc tgtgaattca atctttcact aagtgtttta actactgtgt
    11521 atatatatct ctctcatcaa taaatgtaga ttgcaatttc tcttt
//
