LOCUS       NM_030556               2974 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus solute carrier family 19, member 3 (Slc19a3), mRNA.
ACCESSION   NM_030556
VERSION     NM_030556.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Miyake,K., Yasujima,T., Takahashi,S., Yamashiro,T. and Yuasa,H.
  TITLE     Identification of the amino acid residues involved in the
            species-dependent differences in the pyridoxine transport function
            of SLC19A3
  JOURNAL   J Biol Chem 298 (8), 102161 (2022)
   PUBMED   35724964
  REMARK    GeneRIF: Identification of the amino acid residues involved in the
            species-dependent differences in the pyridoxine transport function
            of SLC19A3.
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Yamashiro,T., Yasujima,T. and Yuasa,H.
  TITLE     Animal species differences in the pyridoxine transport function of
            SLC19A3: Absence of Slc19a3-mediated pyridoxine uptake in the rat
            small intestine
  JOURNAL   Drug Metab Pharmacokinet 44, 100456 (2022)
   PUBMED   35512554
  REMARK    GeneRIF: Animal species differences in the pyridoxine transport
            function of SLC19A3: Absence of Slc19a3-mediated pyridoxine uptake
            in the rat small intestine.
REFERENCE   3  (bases 1 to 2974)
  AUTHORS   Suzuki,K., Yamada,K., Fukuhara,Y., Tsuji,A., Shibata,K. and
            Wakamatsu,N.
  TITLE     High-dose thiamine prevents brain lesions and prolongs survival of
            Slc19a3-deficient mice
  JOURNAL   PLoS One 12 (6), e0180279 (2017)
   PUBMED   28665968
  REMARK    GeneRIF: showed that acute neurodegeneration caused by thiamine
            deficiency is preventable in most parts, and prompt high-dose
            thiamine administration is critical for the treatment of THMD2.
            However, reduction of thiamine should be performed carefully to
            prevent recurrence after recovery of the disease
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2974)
  AUTHORS   Subramanian,V.S., Subramanya,S.B. and Said,H.M.
  TITLE     Relative contribution of THTR-1 and THTR-2 in thiamin uptake by
            pancreatic acinar cells: studies utilizing Slc19a2 and Slc19a3
            knockout mouse models
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 302 (5), G572-G578 (2012)
   PUBMED   22194418
  REMARK    GeneRIF: both mTHTR-1 and mTHTR-2 are involved in carrier-mediated
            thiamin uptake by pancreatic acinar cells.
REFERENCE   5  (bases 1 to 2974)
  AUTHORS   Reidling,J.C., Lambrecht,N., Kassir,M. and Said,H.M.
  TITLE     Impaired intestinal vitamin B1 (thiamin) uptake in thiamin
            transporter-2-deficient mice
  JOURNAL   Gastroenterology 138 (5), 1802-1809 (2010)
   PUBMED   19879271
  REMARK    GeneRIF: THTR-2 is required for normal uptake of thiamin in the
            intestine and can fulfill normal levels of uptake in conditions
            associated with THTR-1 dysfunction.
REFERENCE   6  (bases 1 to 2974)
  AUTHORS   Reidling,J.C., Nabokina,S.M., Balamurugan,K. and Said,H.M.
  TITLE     Developmental maturation of intestinal and renal thiamin uptake:
            studies in wild-type and transgenic mice carrying human THTR-1 and
            2 promoters
  JOURNAL   J Cell Physiol 206 (2), 371-377 (2006)
   PUBMED   16206251
  REMARK    GeneRIF: Intestinal and renal thiamin uptake are developmentally
            regulated during early stages of life, mediated through mTHTR-1 and
            mTHTR-2, and suggest the possible involvement of transcriptional
            regulatory mechanism(s) in this regulation.
REFERENCE   7  (bases 1 to 2974)
  AUTHORS   Ganapathy,V., Smith,S.B. and Prasad,P.D.
  TITLE     SLC19: the folate/thiamine transporter family
  JOURNAL   Pflugers Arch 447 (5), 641-646 (2004)
   PUBMED   14770311
  REMARK    Review article
REFERENCE   8  (bases 1 to 2974)
  AUTHORS   Rajgopal,A., Edmondnson,A., Goldman,I.D. and Zhao,R.
  TITLE     SLC19A3 encodes a second thiamine transporter ThTr2
  JOURNAL   Biochim Biophys Acta 1537 (3), 175-178 (2001)
   PUBMED   11731220
REFERENCE   9  (bases 1 to 2974)
  AUTHORS   Eudy,J.D., Spiegelstein,O., Barber,R.C., Wlodarczyk,B.J., Talbot,J.
            and Finnell,R.H.
  TITLE     Identification and characterization of the human and mouse SLC19A3
            gene: a novel member of the reduced folate family of micronutrient
            transporter genes
  JOURNAL   Mol Genet Metab 71 (4), 581-590 (2000)
   PUBMED   11136550
REFERENCE   10 (bases 1 to 2974)
  AUTHORS   Bettendorff,L.
  TITLE     The compartmentation of phosphorylated thiamine derivatives in
            cultured neuroblastoma cells
  JOURNAL   Biochim Biophys Acta 1222 (1), 7-14 (1994)
   PUBMED   8186267
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161180.3 and BC109154.1.
            
            [WARNING] On Jul 31, 2024 this sequence was replaced by
            NM_030556.3.
            
            On Sep 25, 2008 this sequence version replaced NM_030556.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF271634.1, AK098112.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                AC161180.3         36783-36804
            23-1697             BC109154.1         6-1680
            1698-2974           AC161180.3         61432-62708
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.65 cM"
     gene            1..2974
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /note="solute carrier family 19, member 3"
                     /db_xref="GeneID:80721"
                     /db_xref="MGI:MGI:1931307"
     exon            1..79
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            80..209
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            210..364
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1678
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /note="thiamine transporter 2; solute carrier family 19
                     (sodium/hydrogen exchanger), member 3; thTr-2"
                     /codon_start=1
                     /product="thiamine transporter 2 precursor"
                     /protein_id="NP_085033.2"
                     /db_xref="CCDS:CCDS15101.1"
                     /db_xref="GeneID:80721"
                     /db_xref="MGI:MGI:1931307"
                     /translation="MDSSCRTPPSNSWVYPTVILCLFGFFSMFRPSEAFLIPFLSEPS
                     KNLTSPEMTNEILPVWTYSYLATLPPVFVLTDYLRYKPVIMLHVVAFATSYLFLLFGQ
                     GVMLMQTAEFFFGVVSATEIAYFAYIYSMVSPEHYQKVSSYCRSITLVAYTAGSVLAQ
                     LLVSLTNLPYSSLFYISLACVSVAFFFSLFLPMPKKSMFFHAKSDRDDCPKPLEQCTV
                     PKEAQSNRTHSELFANSKNLEDREMSNPDPENSALRHFAHWFQDLKECYSSKHLVYWS
                     LWWAFATAGYNQILNYVQVLWEHKAPSQDSSIYNGAVEAIATFGGALASFSVGYLKVN
                     WDLLGELGLAVFSAVIAGSLFLMNYSRSIWVCYAGYLLVKSSYSFLITIAVFQIAVNL
                     SLERYALVFGIDTFIALVIQTIMTMIVVDQRGLQLPVTTQFLVYGSYFAVIAGVFLMR
                     SIYILCSAKCRKEVQNLATTRSPNEPHPQEPSNVSTKF"
     exon            365..1172
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1365
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1507
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..2974
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2957..2962
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /note="hexamer: AATAAA"
     polyA_site      2974
                     /gene="Slc19a3"
                     /gene_synonym="A230084E24Rik; ThTr2"
                     /note="major polyA site"
ORIGIN      
        1 tctttacttg agatcgccga cccacatagc tgaaagtgct acatatccgg gacaagtgag
       61 cagcactagc tttctatagc caacacatct gttgggacct acttctacag atcagcttaa
      121 tcaactagca tcgtgggact gaacaactac tagattctcc ggcttcccat ccacacctgc
      181 ccattgttgg gtgaattgga ctacagactc catggactcc agctgcagaa ctccaccgag
      241 caactcttgg gtttatccca ctgtgatact ctgcttgttt gggtttttct ccatgttcag
      301 gccttcagag gcattcttaa tcccattcct gtcagaaccc agcaagaatc tcaccagccc
      361 agagatgaca aatgagatcc ttcctgtttg gacatattct tacctggcaa cgctgccacc
      421 cgtgtttgtc ctcaccgact acctgcgcta caaaccagtc atcatgctac acgttgtggc
      481 cttcgccact agctacctgt ttctcttgtt tggccaaggt gtgatgctca tgcaaacagc
      541 tgagttcttt tttggggttg tctcggccac agagatagcc tactttgcct acatatacag
      601 catggtcagc ccagaacact atcagaaagt gagcagctac tgtcggagta tcacactggt
      661 ggcctacaca gcaggctcag tgttagccca gctcctggta tccctgacga acctgccata
      721 ctctagcctt ttttatatat ccttggcctg tgtctctgtg gctttctttt tctcactttt
      781 tctaccaatg cctaagaaga gcatgttttt ccatgcaaag tccgatagag acgattgtcc
      841 aaagccactg gaacaatgta ctgtgcccaa ggaagcccag agcaacagaa ctcactcaga
      901 attgtttgcc aattcaaaga atctagagga cagagagatg agcaacccag atccagaaaa
      961 ttcagctttg agacactttg cgcattggtt ccaggatctg aaggagtgct actcctcaaa
     1021 gcaccttgtt tactggtccc tctggtgggc ttttgccaca gcaggttata accaaatctt
     1081 gaactatgtt caagtcctat gggaacacaa ggcaccctcc caagattctt ccatctataa
     1141 tggagcagta gaggccatag caacatttgg aggagctttg gcttcattct cagtgggcta
     1201 tctgaaggtc aactgggatc ttctcggaga gctgggtctg gctgtcttct cagctgtcat
     1261 cgcaggatct ctgtttctca tgaattactc acgcagcatc tgggtgtgct atgctgggta
     1321 tctactggtc aagtcgagct attcgtttct tataaccatc gcagtctttc agattgcagt
     1381 taacctgagc ttagaacgtt atgccctggt gttcggcata gacaccttta tcgccttggt
     1441 gattcagacc atcatgacta tgattgtggt agatcaaaga gggctccaac tgccagtcac
     1501 tactcagttt ttagtttatg ggagttactt cgctgtcatt gcgggggtct tcctaatgag
     1561 aagcatatac attctctgct cagctaagtg ccgaaaggaa gtacagaacc ttgctaccac
     1621 ccggagccct aatgagccac acccacaaga gccaagcaat gtgtcaacaa agttctaacc
     1681 caattacatc aattcccgtc acaaatgggc aggattttaa gatgggcagc cagacatgct
     1741 ttgccacgca caggcacact ttatcagtac agattccaaa accacaacca aacttcagtc
     1801 tcaggctaac tgcccagcta actgcaagca ctccgtatat ggccatggca gctgtattag
     1861 ttactttctt gatgctatga caaaatatct aacaagaagc gactcaatgg aggaacttaa
     1921 tatcttagtt cacagtttga agggatacat tccattacaa gagggaagac atgactgcag
     1981 gataaaggag ctactggaca cattgcatct tcaatcaaga agtaaagaac aatgaaacct
     2041 ggtgttcaga tcactctctc ctttttattc aacgcactac cccagccaag gggatggtgc
     2101 tgcccacatt tccagtgaat cttcccacct cagtgaacct aatacagata taccctcaca
     2161 gatattcaaa gaggcgtatg tcatcagatt ccagatcttg tcaaattgac aatattaacc
     2221 accaatatta acagctgaca acccttgttt ggggtcccca tgaatatcaa gaacttggaa
     2281 attctagttc tgttaagttc taagaacaac caatgggcgt cgaaacaagc cataaagaaa
     2341 ctccctaagg atgacgatcc cccaagtgag cacagatacc aacagagctg acctacaatt
     2401 ggcttatgta ctttgtggat ctaaaaaggc agcgcctggg atgattttcc tcctgtttcg
     2461 ctaagtgtac ttctgttgca acctccagtg ctctgaagga atgactaggt ttggtcccat
     2521 tgctgagagt tcctcttcaa cactgaaaat accatcgttc tactcaaagg ttgttactgg
     2581 atagtctcac tgttctcatc tttttcatct tctgtgtcct cctggacttt gtgtgatcac
     2641 tcagacgctc ctgtatgccc atggaatgct ctcccatcta ttcccagtgg ccagggaaag
     2701 atgattcccc ctcttcccac catgacccta gataagtgtt tcattgatgt agaacacttc
     2761 tgaaggaact tccacagctt tccagcagct tttatacata agaaaacact tacagaaatt
     2821 aaatactaat tgtttaattt atttatatgt ttggtgctaa agatcaaatc cagggatcat
     2881 actagataag cattctgtca ctaagatata tccttggccc catggattta atttttaaat
     2941 gccagagatc atgttcaata aaatattaaa atta
//
