LOCUS       NM_001408212            2966 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 18, subfamily B, member 1
            (Slc18b1), transcript variant 4, mRNA.
ACCESSION   NM_001408212
VERSION     NM_001408212.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Fredriksson,R., Sreedharan,S., Nordenankar,K., Alsio,J.,
            Lindberg,F.A., Hutchinson,A., Eriksson,A., Roshanbin,S.,
            Ciuculete,D.M., Klockars,A., Todkar,A., Hagglund,M.G.,
            Hellsten,S.V., Hindlycke,V., Vastermark,A., Shevchenko,G.,
            Olivo,G., K,C., Kullander,K., Moazzami,A., Bergquist,J.,
            Olszewski,P.K. and Schioth,H.B.
  TITLE     The polyamine transporter Slc18b1(VPAT) is important for both short
            and long time memory and for regulation of polyamine content in the
            brain
  JOURNAL   PLoS Genet 15 (12), e1008455 (2019)
   PUBMED   31800589
  REMARK    GeneRIF: Data suggest that removal of Slc18b1 leads to reduction of
            polyamine contents in neurons, resulting in reduced GABA signaling
            due to long-term reduction in glutamatergic signaling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2966)
  AUTHORS   Park,S.J., Kim,B., Choi,S., Balasubramaniam,S., Lee,S.C., Lee,J.Y.,
            Kim,H.S., Kim,J.Y., Kim,J.J., Lee,Y.A., Kang,N.Y., Kim,J.S. and
            Chang,Y.T.
  TITLE     Imaging inflammation using an activated macrophage probe with
            Slc18b1 as the activation-selective gating target
  JOURNAL   Nat Commun 10 (1), 1111 (2019)
   PUBMED   30846702
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2966)
  AUTHORS   Takeuchi,T., Harada,Y., Moriyama,S., Furuta,K., Tanaka,S.,
            Miyaji,T., Omote,H., Moriyama,Y. and Hiasa,M.
  TITLE     Vesicular Polyamine Transporter Mediates Vesicular Storage and
            Release of Polyamine from Mast Cells
  JOURNAL   J Biol Chem 292 (9), 3909-3918 (2017)
   PUBMED   28082679
  REMARK    GeneRIF: VPAT is expressed and is responsible for vesicular storage
            of spermine and spermidine in novel secretory granules that differ
            from histamine- and serotonin-containing granules and is involved
            in vesicular release of these polyamines from mast cells
REFERENCE   5  (bases 1 to 2966)
  AUTHORS   Coulpier,F., Le Crom,S., Maro,G.S., Manent,J., Giovannini,M.,
            Maciorowski,Z., Fischer,A., Gessler,M., Charnay,P. and Topilko,P.
  TITLE     Novel features of boundary cap cells revealed by the analysis of
            newly identified molecular markers
  JOURNAL   Glia 57 (13), 1450-1457 (2009)
   PUBMED   19243017
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160147.6 and AC153973.33.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.272938.1,
                                           SRR7345562.4490564.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AC160147.6         135770-136099
            331-470             AC160147.6         137538-137677
            471-566             AC160147.6         142550-142645
            567-640             AC160147.6         142980-143053
            641-788             AC160147.6         144685-144832
            789-945             AC153973.33        112670-112826       c
            946-1082            AC153973.33        107186-107322       c
            1083-1184           AC153973.33        104454-104555       c
            1185-1276           AC153973.33        102710-102801       c
            1277-1372           AC153973.33        100597-100692       c
            1373-1447           AC153973.33        98855-98929         c
            1448-1541           AC153973.33        97575-97668         c
            1542-1594           AC153973.33        97420-97472         c
            1595-2966           AC153973.33        95650-97021         c
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 11.12 cM"
     gene            1..2966
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /note="solute carrier family 18, subfamily B, member 1"
                     /db_xref="GeneID:76306"
                     /db_xref="MGI:MGI:1923556"
     exon            1..330
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..1661
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /note="isoform 4 is encoded by transcript variant 4;
                     MFS-type transporter C6orf192 homolog; solute carrier
                     family 18 member B1; vesicular polyamine transporter;
                     MFS-type transporter SLC18B1"
                     /codon_start=1
                     /product="MFS-type transporter SLC18B1 isoform 4"
                     /protein_id="NP_001395141.1"
                     /db_xref="GeneID:76306"
                     /db_xref="MGI:MGI:1923556"
                     /translation="MGLGPVSLGYNGLACFVVVAAAAVVVSETFKQGDDPGGSTRETS
                     RRLSREQIFVLVSAASMNLGCMMTYSILGPFFPKEAEKKGASNTMIGMIFGCYALFEL
                     LASLVFGKYLVHIGAKFMFIAGMFVSGGVTILFGVLDQLPEGPIFIAMCFLVRIVDAI
                     GFGAAITASSSILAKAFPNNVATVMGSLEVFSGLGLVAGPPLGGLLYQSFGYEVPFIF
                     LGCIVLLMIPLNLYILPSYESDPGKQSFWKLVTLPKMGLLAFVIISLSSCFGFLDPTL
                     SLFVMEKFSLSTGYVGLVFLGLSLSYAISSPLFGLLSDKMPTLRKWLLVFGNLITAGC
                     YMLLGPVPLLHIKSQLWLLVLVLVVNGISAGMSIIPTFPEMLSCAYANGFEDSISTLG
                     LVSGLFGAMWSVGAFMGPILGGFLCEKIGFEWAAAMQGLWTLLSGVSMALFYLWEDST
                     ARRRSKAQNSLGTEEERAALLPNDT"
     exon            331..470
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            471..566
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            567..640
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            641..788
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            789..945
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1082
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1184
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1276
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1372
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1447
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1541
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1594
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..2966
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2948..2953
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /note="hexamer: AATAAA"
     polyA_site      2966
                     /gene="Slc18b1"
                     /gene_synonym="1110021L09Rik; VPAT"
                     /note="major polyA site"
ORIGIN      
        1 aagctcccgt gggtccccgg ttccaaggat cagcgaggtt tcttgtcccg gctcaagtct
       61 tcacctctgg accggaatgc ggagtccgat ccccgcagct cgcccgctgc caccgcacct
      121 cgcccgctga gcctggagcg ccggaagacc ccgagagcgg ctcatggacg aggcgggctc
      181 cccggctcct gcagggacag gaggaggtga ctgttttcag cgcgtagctc gccatgggct
      241 tagggcctgt gtctttagga tataatggct tagcttgttt tgttgttgtt gctgccgctg
      301 ctgttgttgt ttctgagacc ttcaagcagg gtgatgaccc tggaggaagt acaagagaga
      361 catcccggcg actgtcaaga gaacagattt ttgtgctggt atcagcagca agcatgaact
      421 tgggttgcat gatgacctat tccatccttg gaccattttt ccctaaggag gcagaaaaga
      481 agggagccag caacaccatg attggaatga tcttcggatg ctatgcgtta tttgaattgc
      541 tggcatcttt ggtgtttgga aaataccttg tacacattgg agccaaattt atgtttatag
      601 caggaatgtt tgtctcagga ggagttacaa ttctctttgg tgtgttggat caacttccgg
      661 aaggaccgat attcattgcc atgtgttttc tggtgagaat agtagacgcc ataggctttg
      721 gtgcggccat aacagcatcg tcatccatcc ttgctaaggc tttcccaaac aatgtggcta
      781 cagtcatggg aagtcttgag gttttttctg gactggggct ggtagcaggc ccccctttag
      841 gtggcctttt gtatcaatcc tttggctacg aggtaccctt tatttttctg gggtgcatag
      901 tcctgctgat gataccactc aacctataca ttttgcctag ttatgagtcc gatccaggga
      961 agcagtcctt ctggaaactt gtcaccttac ccaagatggg cctcctagcc ttcgtgatca
     1021 tctctctcag ctcctgcttc ggcttcctgg acccaaccct gtctctcttt gtcatggaga
     1081 agttcagtct gtcaactgga tacgtgggcc tggtcttcct gggtctgtct ctatcctacg
     1141 ccatttcttc gccgctgttt ggtctactca gcgataagat gcctactcta aggaaatggc
     1201 ttctggtttt tggaaactta attacagctg ggtgctacat gctcttagga cctgttcctc
     1261 ttctgcacat caaaagtcag ctctggcttc ttgtgttggt gttggttgta aatggcatct
     1321 ccgctggaat gagcatcatc ccgaccttcc cagagatgct cagctgtgca tatgcaaatg
     1381 gatttgaaga cagcataagt acgctgggac ttgtatctgg tctttttggt gccatgtggt
     1441 cagttggagc tttcatggga cctatcctgg gcggatttct gtgtgagaaa attggtttcg
     1501 agtgggcagc agctatgcaa ggcttatgga ctctgctaag tggtgtttcg atggccttat
     1561 tttatctctg ggaggactca acggcaagaa gaagatctaa agctcagaac agccttggca
     1621 cagaggagga acgagctgct ctcttgccca atgacaccta gccttgagag ctctggattg
     1681 atccagatcc agagacaggt gccttgactg tgactgcagt ggctggagga gcttcctgtg
     1741 atccgtgctt cctgcaccca caccctggaa gtgtcaagct gcatttacat gaccgggagc
     1801 cattctctgc ttcctgtagt tccagaaagc tggctcacca aggcaacatc tatgagaaag
     1861 ggcaaaccag ccaaccggag cagtgtttct aaaagaccga ccttgcccgt caggttttta
     1921 ttgaccgtct ttgctttctt attcttgtgt tcacttttca ccccaagttc actccaaagt
     1981 gttgtggatg tacgtcttaa aatgaagcta tttaattcac attgaacata taaaatagaa
     2041 cgaataattt catgaaaccg gttttgaaag atggccttcc tctacactga agtttgtact
     2101 cgtcaaaaag gcaaaattcc tgcctaccat ataaaaactc cagagcattt cttttttttt
     2161 tcttcaacat ttttttttaa ttaggtattt tcctcattta cattcccaat gctatcccaa
     2221 aagtccccca tacctttccc cccacgcccc tacccaccca ctcccacttt tggccctggc
     2281 gttcccctgt actggggcat ataaagtttg tatgtccaat gggcctctct ttccagtgat
     2341 ggccgactag gccatcttct gctacatatg cagctagagt caagagctcc agggtactgg
     2401 ttagttcata attttgttcc acctataggg ttgcagatcc cttcagctcc ttgggtattt
     2461 tctctagctc ctccattggg ggccctgtga tccatccaat agttgactgt gagcatccac
     2521 ttatatgttt gctaggcccc agtccagagc atttcttggc tttcagtttt tctaaaaatg
     2581 ttcgcatggc aggtccaaga aaaaacaatg catgcacttg agtcctgtga catttccttt
     2641 catgatcttg gcttctacac attatttgca taacattgtt tgtttctatt cttagcatgt
     2701 ctttgtttta gtctcattat ttgtttgctt attgtgttta ctacttttcg tactcttggg
     2761 aaatgtgttc catcacgagg cagggccaca cctgcctccg cccaggtaag cgagtcgtgt
     2821 gctccacgtt ggagaaacac tccaacttca cagaaaatcc ttgctgctac ttgaagttag
     2881 aatgtcaatt ttgtacctcc tgattcattt ccatattgta gtagctacat ttccttttga
     2941 agagaataat aaatgatttt tgtcta
//
