LOCUS       NM_021712               2414 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus solute carrier family 18 (vesicular monoamine), member
            3 (Slc18a3), mRNA.
ACCESSION   NM_021712
VERSION     NM_021712.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Chen,X., Tober,J., Dominguez,M., Tang,A.T., Bockman,J., Yang,J.,
            Mani,S., Lee,C.N., Chen,M., Thillaikumaran,T., Mericko-Ishizuka,P.,
            Mainigi,M., Speck,N.A. and Kahn,M.L.
  TITLE     Lineage tracing studies suggest that the placenta is not a de novo
            source of hematopoietic stem cells
  JOURNAL   PLoS Biol 23 (1), e3003003 (2025)
   PUBMED   39874373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   N'Guetta,P.Y., McLarnon,S.R., Tassou,A., Geron,M., Shirvan,S.,
            Hill,R.Z., Scherrer,G. and O'Brien,L.L.
  TITLE     Comprehensive mapping of sensory and sympathetic innervation of the
            developing kidney
  JOURNAL   Cell Rep 43 (10), 114860 (2024)
   PUBMED   39412983
REFERENCE   3  (bases 1 to 2414)
  AUTHORS   Kc,R., Lopez de Boer,R., Lin,M., Vagnozzi,A.N., Jeannotte,L. and
            Philippidou,P.
  TITLE     Multimodal Hox5 activity generates motor neuron diversity
  JOURNAL   Commun Biol 7 (1), 1166 (2024)
   PUBMED   39289460
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2414)
  AUTHORS   Hsu,H.C., Hsu,S.P., Hsu,F.Y., Chang,M. and Chen,J.A.
  TITLE     LncRNA Litchi is a regulator for harmonizing maturity and resilient
            functionality in spinal motor neurons
  JOURNAL   iScience 27 (3), 109207 (2024)
   PUBMED   38433925
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2414)
  AUTHORS   Lowenstein,E.D., Ruffault,P.L., Misios,A., Osman,K.L., Li,H.,
            Greenberg,R.S., Thompson,R., Song,K., Dietrich,S., Li,X.,
            Vladimirov,N., Woehler,A., Brunet,J.F., Zampieri,N., Kuhn,R.,
            Liberles,S.D., Jia,S., Lewin,G.R., Rajewsky,N., Lever,T.E. and
            Birchmeier,C.
  TITLE     Prox2 and Runx3 vagal sensory neurons regulate esophageal motility
  JOURNAL   Neuron 111 (14), 2184-2200 (2023)
   PUBMED   37192624
REFERENCE   6  (bases 1 to 2414)
  AUTHORS   Naciff,J.M., Behbehani,M.M., Misawa,H. and Dedman,J.R.
  TITLE     Identification and transgenic analysis of a murine promoter that
            targets cholinergic neuron expression
  JOURNAL   J Neurochem 72 (1), 17-28 (1999)
   PUBMED   9886050
REFERENCE   7  (bases 1 to 2414)
  AUTHORS   Sang,Q. and Young,H.M.
  TITLE     The origin and development of the vagal and spinal innervation of
            the external muscle of the mouse esophagus
  JOURNAL   Brain Res 809 (2), 253-268 (1998)
   PUBMED   9853118
REFERENCE   8  (bases 1 to 2414)
  AUTHORS   Naciff,J.M., Misawa,H. and Dedman,J.R.
  TITLE     Molecular characterization of the mouse vesicular acetylcholine
            transporter gene
  JOURNAL   Neuroreport 8 (16), 3467-3473 (1997)
   PUBMED   9427309
REFERENCE   9  (bases 1 to 2414)
  AUTHORS   Sang,Q. and Young,H.M.
  TITLE     Development of nicotinic receptor clusters and innervation
            accompanying the change in muscle phenotype in the mouse esophagus
  JOURNAL   J Comp Neurol 386 (1), 119-136 (1997)
   PUBMED   9303529
REFERENCE   10 (bases 1 to 2414)
  AUTHORS   Carroll,P.T.
  TITLE     The effect of the acetylcholine transport blocker
            2-(4-phenylpiperidino) cyclohexanol (AH5183) on the subcellular
            storage and release of acetylcholine in mouse brain
  JOURNAL   Brain Res 358 (1-2), 200-209 (1985)
   PUBMED   4075114
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167565.1.
            
            On Jan 23, 2019 this sequence version replaced NM_021712.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK158592.1, BC137705.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2414              AC167565.1         193336-195749       c
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.4 cM"
     gene            1..2414
                     /gene="Slc18a3"
                     /gene_synonym="VAChT; VAT"
                     /note="solute carrier family 18 (vesicular monoamine),
                     member 3"
                     /db_xref="GeneID:20508"
                     /db_xref="MGI:MGI:1101061"
     exon            1..2414
                     /gene="Slc18a3"
                     /gene_synonym="VAChT; VAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..2019
                     /gene="Slc18a3"
                     /gene_synonym="VAChT; VAT"
                     /note="solute carrier family 18 member 3"
                     /codon_start=1
                     /product="vesicular acetylcholine transporter"
                     /protein_id="NP_068358.2"
                     /db_xref="CCDS:CCDS79291.1"
                     /db_xref="GeneID:20508"
                     /db_xref="MGI:MGI:1101061"
                     /translation="MEPTAPTGQARAAATKLSEAVGAALQEPQRQRRLVLVIVCVALL
                     LDNMLYMVIVPIVPDYIAHMRGGSESPTLISEVWEPTLPPPTLANASAYLANTSASPT
                     AAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLG
                     VMFASTVMFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVAL
                     AFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANL
                     PVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPA
                     FVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCF
                     GIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQ
                     LSLGMGLANLLYAPVLLLLRNVGLLTRSRSERDVLLDEPPQGLYDAMRLREVQGKDGG
                     EPCSPPGPFDGCEDDYNYYSRS"
     regulatory      2391..2396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc18a3"
                     /gene_synonym="VAChT; VAT"
                     /note="hexamer: AATAAA"
     polyA_site      2413
                     /gene="Slc18a3"
                     /gene_synonym="VAChT; VAT"
                     /note="major polyA site"
ORIGIN      
        1 ggtggaggaa gaggcaagag cggacgcgcg gggagggctg gagagacggc gggcggcggc
       61 agcatgcccc tgggcgggtg cacacggcct ctctgcgcgg caggggctgc tcctgctctc
      121 tctgggcacc gcgcgtccag tctcccgcct cagcccctcg gcttgccggc ctccgcggtt
      181 gcatccgaaa cagcggcaac tggtccctgg agcatctaag agcagcggcg ccgcgcggga
      241 caatccttga tttttttttt tttttctcct gagctcgggg atatgaaccc cacagccacc
      301 tgaagcacag ggggcgctac gcggctagga ccgcgccccc gaagtaccgt accctagcct
      361 ctgcactgcg ggacgccgac acccgactcc ggtggaggca tcttaggaag agcagcgggt
      421 aggggcatgg aacccaccgc gccaaccggt caggcccggg cggcggccac caagctgtcg
      481 gaagcagtgg gagccgcgct acaagagccc cagaggcagc ggcgcctggt gctggtcatc
      541 gtgtgcgttg cactgttact ggacaacatg ttgtacatgg tcatcgtgcc cattgttccc
      601 gactatatcg ctcacatgcg cgggggcagc gagagcccta ccctgatctc tgaggtgtgg
      661 gaacccaccc tgccgccgcc cactctggct aatgccagcg cctacttggc caacacctcg
      721 gcgtccccga cggctgccgg gtcggctcgg tcaatcctgc ggcctcgcta ccccacagaa
      781 agtgaagatg tgaagatagg cgtgctattt gcctccaagg ccatcctgca gcttctggtg
      841 aaccccttaa gcgggccttt cattgatcgc atgagctacg acgtgccgct gcttattggc
      901 ctgggcgtca tgttcgcctc cacagtcatg tttgcctttg cagaagacta tgccacgctc
      961 ttcgctgcgc gcagtctaca aggcctgggc tcagccttcg cggacacgtc tggcattgcc
     1021 atgatcgccg acaagtatcc cgaggagcct gagcgcagtc gtgctctggg cgtggcgcta
     1081 gccttcatca gctttggaag cctagtggcg ccaccgtttg ggggcatcct ctacgagttc
     1141 gcgggcaagc gtgtaccctt tctagtgctc gccgccgtgt cgctcttcga cgcactccta
     1201 ctcctggcgg tggctaagcc tttctcagct gcggctcggg cgcgggccaa cctgcccgtg
     1261 ggcacaccta tccatcgcct catgctagac ccttacatcg ctgttgtagc cggagcgctc
     1321 acaacttgta acattcccct tgcgtttctc gagcccacca tagccacgtg gatgaagcac
     1381 acaatggcgg catccgagtg ggagatgggc atggtttggc tgccggcttt cgtgccacac
     1441 gtgttaggcg tctatctcac cgtgcgtcta gcggcgcgct atccacacct gcagtggctg
     1501 tatggcgctc tcgggctagc ggtaatcgga gtgagctctt gcgtcgtacc tgcctgccga
     1561 tccttcgcgc ccttagtggt ctcgctctgc ggcctctgct tcggcatcgc gttggtggac
     1621 acggcgctcc tacccacgct cgcctttctg gtggacgtgc gccacgtatc agtctatggc
     1681 agtgtctatg ccatagctga catctcctat tctgtggcct acgctctcgg gcccatagta
     1741 gcaggccata tcgttcactc acttggcttt gagcagctca gccttggcat gggcctggcc
     1801 aacctgctct acgcaccggt cctattgctc ttgcgtaatg tgggcctcct tacacgctcg
     1861 cgttccgagc gcgatgtgct gcttgatgaa ccgccgcagg gtctgtacga cgcgatgcgc
     1921 ctgcgtgagg tgcagggcaa ggacggcggt gaaccttgta gcccgcctgg cccttttgat
     1981 ggctgtgagg acgactacaa ctactactcc cgcagctagc agacccgctt ctcctccagg
     2041 ccacctaccc tccccattta ggtcaagatg gtcattcttc aagagcgctg gccaactttg
     2101 gcttggggcc cacctcctct aatgagtacc ctagcccgtc ccccgccctg aattcctttg
     2161 ctggaatccc ttctccgtga cccctcccag tctaaccccc tctccaaaca cactcgtatt
     2221 cattgaggga atggatcagg gaggcattgg gctcttggca gaggtgtacc ggtgatcacc
     2281 aaccacctac tgagagcccc caagtacagt catgcatctg tttgtccttc ctgcggatct
     2341 ttccagtgcc aaacttggtc tactacaccc tggtgcctcc ggcctgaatt aataaaccat
     2401 atctgtctga ggag
//
