LOCUS       NM_021301               4007 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus solute carrier family 15 (H+/peptide transporter),
            member 2 (Slc15a2), transcript variant 1, mRNA.
ACCESSION   NM_021301
VERSION     NM_021301.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Nies,A.T., Konig,J., Leuthold,P., Damme,K., Winter,S., Haag,M.,
            Masuda,S., Kruck,S., Daniel,H., Spanier,B., Fromm,M.F., Bedke,J.,
            Inui,K.I., Schwab,M. and Schaeffeler,E.
  TITLE     Novel drug transporter substrates identification: An innovative
            approach based on metabolomic profiling, in silico ligand screening
            and biological validation
  JOURNAL   Pharmacol Res 196, 106941 (2023)
   PUBMED   37775020
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Decourtye,L., McCallum-Loudeac,J.A., Zellhuber-McMillan,S.,
            Young,E., Sircombe,K.J. and Wilson,M.J.
  TITLE     Characterization of a novel Lbx1 mouse loss of function strain
  JOURNAL   Differentiation 123, 30-41 (2022)
   PUBMED   34906895
REFERENCE   3  (bases 1 to 4007)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4007)
  AUTHORS   Shah,K., King,G.D. and Jiang,H.
  TITLE     A chromatin modulator sustains self-renewal and enables
            differentiation of postnatal neural stem and progenitor cells
  JOURNAL   J Mol Cell Biol 12 (1), 4-16 (2020)
   PUBMED   31065682
REFERENCE   5  (bases 1 to 4007)
  AUTHORS   Hu,Y., Song,F., Jiang,H., Nunez,G. and Smith,D.E.
  TITLE     SLC15A2 and SLC15A4 Mediate the Transport of Bacterially Derived
            Di/Tripeptides To Enhance the Nucleotide-Binding Oligomerization
            Domain-Dependent Immune Response in Mouse Bone Marrow-Derived
            Macrophages
  JOURNAL   J Immunol 201 (2), 652-662 (2018)
   PUBMED   29784761
  REMARK    GeneRIF: this study shows that SLC15A2 mediates the transport of
            bacterially derived di/tripeptides to enhance the
            nucleotide-binding oligomerization domain-dependent immune response
            in mouse bone marrow-derived macrophages
REFERENCE   6  (bases 1 to 4007)
  AUTHORS   Teuscher,N.S., Shen,H., Shu,C., Xiang,J., Keep,R.F. and Smith,D.E.
  TITLE     Carnosine uptake in rat choroid plexus primary cell cultures and
            choroid plexus whole tissue from PEPT2 null mice
  JOURNAL   J Neurochem 89 (2), 375-382 (2004)
   PUBMED   15056281
  REMARK    GeneRIF: Studies in transgenic mice demonstrated that PEPT2 was
            responsible for over 90% of carnosine uptake in choroid plexus.
            These findings elucidate the unique role of PEPT2 in regulating
            neuropeptide homeostasis at the blood-cerebrospinal fluid interface
            Erratum:[J Neurochem. 2004 Nov;91(4):1024]
REFERENCE   7  (bases 1 to 4007)
  AUTHORS   Rubio-Aliaga,I., Frey,I., Boll,M., Groneberg,D.A., Eichinger,H.M.,
            Balling,R. and Daniel,H.
  TITLE     Targeted disruption of the peptide transporter Pept2 gene in mice
            defines its physiological role in the kidney
  JOURNAL   Mol Cell Biol 23 (9), 3247-3252 (2003)
   PUBMED   12697824
  REMARK    GeneRIF: PEPT2 is the main system responsible for tubular
            reabsorption of peptide-bound amino acids, although this does not
            lead to major changes in renal excretion of protein or free amino
            acids.
REFERENCE   8  (bases 1 to 4007)
  AUTHORS   Shen,H., Smith,D.E., Keep,R.F., Xiang,J. and Brosius,F.C. 3rd.
  TITLE     Targeted disruption of the PEPT2 gene markedly reduces dipeptide
            uptake in choroid plexus
  JOURNAL   J Biol Chem 278 (7), 4786-4791 (2003)
   PUBMED   12473671
  REMARK    GeneRIF: PEPT2 is responsible for dipeptide uptake in choroid
            plexus tissue
REFERENCE   9  (bases 1 to 4007)
  AUTHORS   Groneberg,D.A., Doring,F., Theis,S., Nickolaus,M., Fischer,A. and
            Daniel,H.
  TITLE     Peptide transport in the mammary gland: expression and distribution
            of PEPT2 mRNA and protein
  JOURNAL   Am J Physiol Endocrinol Metab 282 (5), E1172-E1179 (2002)
   PUBMED   11934684
  REMARK    GeneRIF: Peptide transport in the mammary gland
REFERENCE   10 (bases 1 to 4007)
  AUTHORS   Rubio-Aliaga,I., Boll,M. and Daniel,H.
  TITLE     Cloning and characterization of the gene encoding the mouse peptide
            transporter PEPT2
  JOURNAL   Biochem Biophys Res Commun 276 (2), 734-741 (2000)
   PUBMED   11027540
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117662.13 and AEKQ02018542.1.
            
            On Jul 30, 2020 this sequence version replaced NM_021301.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF111811.1, AK128939.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-308               AC117662.13        264464-264771       c
            309-396             AC117662.13        262081-262168       c
            397-538             AC117662.13        261375-261516       c
            539-631             AC117662.13        255407-255499       c
            632-731             AC117662.13        254351-254450       c
            732-822             AEKQ02018542.1     2116-2206           c
            823-900             AC117662.13        251873-251950       c
            901-983             AC117662.13        242114-242196       c
            984-1070            AC117662.13        241632-241718       c
            1071-1160           AC117662.13        241373-241462       c
            1161-1205           AC117662.13        241239-241283       c
            1206-1238           AC117662.13        239880-239912       c
            1239-1327           AC117662.13        239035-239123       c
            1328-1409           AC117662.13        237589-237670       c
            1410-1544           AC117662.13        236931-237065       c
            1545-1709           AC117662.13        236034-236198       c
            1710-1756           AC117662.13        235859-235905       c
            1757-1867           AC117662.13        234251-234361       c
            1868-1964           AC117662.13        233505-233601       c
            1965-2111           AC117662.13        232802-232948       c
            2112-2216           AC117662.13        232081-232185       c
            2217-4007           AC117662.13        229973-231763       c
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 25.97 cM"
     gene            1..4007
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /note="solute carrier family 15 (H+/peptide transporter),
                     member 2"
                     /db_xref="GeneID:57738"
                     /db_xref="MGI:MGI:1890457"
     exon            1..308
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..2393
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proton-dependent high affinity oligopeptide transporter
                     PepT2; solute carrier family 15 member 2; peptide
                     transporter 2; kidney H(+)/peptide cotransporter"
                     /codon_start=1
                     /product="solute carrier family 15 member 2 isoform 1"
                     /protein_id="NP_067276.3"
                     /db_xref="CCDS:CCDS28156.1"
                     /db_xref="GeneID:57738"
                     /db_xref="MGI:MGI:1890457"
                     /translation="MLEERESKEPAMNPFQKNESKETLFSPVSTEEMLPGPPSPPKKS
                     TPKLFGSSYPLSIAFIVVNEFCERFSYYGMKAVLTLYFLYFLHWNEDTSTSVYHAFSS
                     LCYFTPILGAAIADSWLGKFKTIIYLSLVYVLGHVFKSLGAIPILGGKMLHTILSLVG
                     LSLIALGTGGIKPCVAAFGGDQFEEEHAEARTRYFSVFYLSINAGSLISTFITPMLRG
                     DVKCFGEDCYALAFGIPGLLMVLALVVFAMGSKMYRKPPPEGNIVAQVTKCIWFAICN
                     RFRNRSEDIPKRQHWLDWAAEKYPKHLIMDVKALTRILFLYIPLPMFWALLDQQGSRW
                     TLQANKMDGDLGFFVLQPDQMQVLNPFLVLVFIPLFDLVIYRLISKCGVNFSSLRKMA
                     VGMILACLAFAVAALVEIKINGMIHPQPASQEIFLQVLNLADGEIEVTVQGNRNNPLL
                     VESISSFQNTTHYSKLRLETKSQDLHFHLKYNNLSVHNEYSVEEKNCYQLVVHENGES
                     LSSMLVKDTGIKPANGMTAIRFINTLHKDMNISLDANAPLSVGKDYGVSEYRTVQRGK
                     YPAVHCETEDNVFSLNLGQLDFGTTYLFVITNITNRGLQAWKAEDIPANKLSIAWQLP
                     QYVLVTAAEVMFSVTGLEFSYSQAPSSMKSVLQAAWLLTVAVGNIIVLIVAQFSGLVQ
                     WAEFVLFSCLLLVVCLIFSVMGYYYVPLKSEGIHEATEKQIPHIQGNMINLETKNTRL
                     "
     exon            309..396
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            397..538
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..631
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            632..731
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            732..822
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            823..900
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            901..983
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1070
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1160
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1205
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1238
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1327
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1409
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1544
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1709
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1756
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1757..1867
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1964
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2111
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2216
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..4007
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3971..3976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /note="hexamer: AATAAA"
     polyA_site      3996
                     /gene="Slc15a2"
                     /gene_synonym="8430408C16Rik; Pept2"
                     /note="major polyA site"
ORIGIN      
        1 cagcgtgcag cccctctctt ttcctatagc ttcaagttct gctctaaggc aggcagccac
       61 tgaaactgac acccacaggg ttaaacctat ttgctgtagc tatccctcct ccccctagtc
      121 ccccttttca ctgtaggctg gcagcagtcc tagctgccta ctgaagccaa atgcttgagg
      181 agagagagag taaggagcca gccatgaatc ctttccagaa aaatgagtcc aaggaaacgc
      241 tcttttcacc tgtctctact gaagagatgc tgcctggacc tccaagccct ccaaagaagt
      301 caactccgaa gctcttcggc tctagctacc cactcagcat cgccttcatt gtggtgaacg
      361 aattctgcga gcgattttcc tattatggca tgaaagctgt gttgaccttg tatttcctgt
      421 acttcctgca ctggaatgaa gacacgtcca catctgtcta ccatgccttc agcagcctct
      481 gctatttcac ccccatcttg ggagcagcga ttgctgactc ctggctggga aaattcaaga
      541 caatcatcta tctgtccctg gtgtatgttc ttggccatgt attcaagtct ttgggtgcca
      601 taccaatact gggaggaaaa atgctacata caatcttatc attggttggc ctgagtctca
      661 tagctctggg aacaggaggt atcaaaccct gtgtggcagc ttttggcgga gaccagtttg
      721 aagaggaaca tgcagaggca cggactagat acttctcggt cttctacctc tccatcaatg
      781 cagggagctt gatttctacg ttcatcacac ccatgctaag aggggatgtg aagtgttttg
      841 gcgaagactg ctatgcattg gcttttggga ttccaggatt gctcatggtg ctggcacttg
      901 ttgtgtttgc aatgggaagc aagatgtaca gaaaaccgcc tcctgaaggg aacatagtgg
      961 ctcaagttac caaatgtata tggtttgcta tctgcaaccg cttcaggaac cgttctgagg
     1021 acattccaaa gcgacaacat tggctagact gggcagcaga aaaataccca aagcacctca
     1081 ttatggacgt gaaggcactg accaggatac tgttccttta tatcccactg cccatgttct
     1141 gggctctttt ggaccaacag ggctcacggt ggactctgca agctaacaaa atggatggcg
     1201 atttgggatt ttttgtactt cagccggatc agatgcaggt gctaaatccc tttctggtcc
     1261 ttgtcttcat cccattgttt gaccttgtca tttatcggct gatctccaag tgcggagtta
     1321 acttctcgtc actcaggaaa atggctgttg gtatgatcct agcgtgcttg gcatttgcag
     1381 ttgcagcgct tgtagagata aaaataaatg gaatgatcca tcctcagcca gcttcccaag
     1441 agatttttct gcaagtcctg aacttggcag atggtgaaat agaggtgaca gtgcagggaa
     1501 acagaaataa tcctctcttg gtggagtcca tcagctcttt tcagaacaca acacactatt
     1561 cgaaactacg cctggagaca aaaagccaag acttacattt tcatctgaag tacaacaact
     1621 tgtctgtcca caatgagtac tctgtagagg agaagaactg ttaccagttg gtggttcatg
     1681 agaatggaga aagtctctcc agtatgctgg taaaggacac aggaatcaaa ccagccaatg
     1741 ggatgacagc catcaggttt attaacactt tgcataaaga catgaatatt tccttggatg
     1801 ccaatgctcc tctcagcgtg ggcaaagact atggagtgtc tgagtacaga actgtgcaaa
     1861 gaggaaagta tcctgcagtg cattgtgaaa cggaagacaa tgtcttttct ctcaatttag
     1921 gtcaactaga ctttggtaca acatatttgt ttgtgatcac taatatcacc aaccggggtc
     1981 ttcaggcctg gaaggcagaa gacattccag ccaataaact atccattgca tggcagctac
     2041 cacagtatgt cctggttaca gcagcagagg tcatgttttc tgtcacagga cttgaatttt
     2101 cttattctca ggcaccatct agcatgaaat ctgtgctcca ggcagcctgg ctgttgaccg
     2161 ttgcagttgg gaatattatt gtgctgatcg tggcacagtt cagtggcctg gtgcagtggg
     2221 ctgaattcgt tttgttttcc tgcctcctgc tggtggtctg cctgatcttc tccgtcatgg
     2281 gctactacta tgttcctcta aagtcagagg gtatccatga ggcaacagaa aagcagattc
     2341 ctcacatcca ggggaatatg atcaacctag aaaccaagaa tacaaggctc tgatgactca
     2401 ctggatggag actgacccct ggctctgata tcagccatcg cttatcttca agcaacaatt
     2461 ttttcccccc tctggccagg ttagagaagg agacagcatt tatctgagtg gattgcctcc
     2521 attttctacc acaatagagg caattttagg acaaggtttt caggtatatc tttaaacaag
     2581 gatccaagac tccgtgtcct acaggctatt gataaactca gtaaatatta ggcaatgtct
     2641 ctcaagctgg cccagtatct ccaaatgccc aggaaagtac aaatgtgtag tagagataag
     2701 tctcatggca tgctaagttt ggaggactcc tttatcaggt atttggaacc tatagctcta
     2761 cttgttgata tgttggttta gggccaaaat gacagaatac tggtatttca attttcttta
     2821 tcagtaatat aactttgcta ttcatgagga tgtcaaaaat gttacatacg gtatgaccta
     2881 atccagaggc tagctcgtca gctagaaagt ttaatatgat ttttagatgg ccgactatag
     2941 aatggaattt tatgtacaca tggaagatac ctctttctga ttaccacact ggtttatcta
     3001 cttatccaaa aaccctttct cggtactgac agatcctgga aaccataatt aaagacagta
     3061 aggaaagtca tggggacaca ggaatgcata taaactattc attctcttgt actgcacgcc
     3121 cctctaaaga actctcttgt tccgggcctg ggctgtgctc ctccgtgaac atctgtctat
     3181 gaaactaact atagctttgc aacccagcct tttcagtgtt gctgggacag cttggtatta
     3241 ttattatatc ttactttgct tattacagca aatcttcagt aacaaaatgt tttttctaac
     3301 ccaacttcct ttaatttttt tttctattta gcttctttgt tgtgtatttt tatttttccc
     3361 gtaatttttg tttacatttc tccctttggt cttagaaata cgccctgctt atggaaaagg
     3421 gataatacta acatcctggg tttaaagctc taaattgtaa tattcagcaa atggacaagg
     3481 acctgcatga gaaacttatt aaaatctgtg ggggtgggtg gagcaggtgg agtttttgtg
     3541 tgtgtgaaaa aaaaagaggg gagttgagaa acattatctc acatgtaata catgggtcag
     3601 ggaacgagct tgggaatatt aggactatgt catgtgttcc taggagaaaa cgttcacggc
     3661 ccctgtatga aagacctcat ttctgttcct tttactacac ggtttccgtc tatagccatg
     3721 ttgatcgcac attgttaata atgtaatatg ttcttaagat aatctgctgc agtccctcaa
     3781 gctttaaata cttaagtatt caactactaa gattttagaa agcataaacg aggtcgcaac
     3841 actatctcct ttccccagac aactgctaag aacctgtccc aactcctttt gaagatgttc
     3901 atttttaaga agctttagct caaagctatt tggtgaattt tgttttaaag ggcgttaaga
     3961 ctgatctgtg aataaaagtt tttatgtaca agtaaatttg ggaaatg
//
