LOCUS       NM_028122               3675 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus solute carrier family 14 (urea transporter), member 1
            (Slc14a1), transcript variant 2, mRNA.
ACCESSION   NM_028122
VERSION     NM_028122.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Jones,A.C., Pinki,F., Stewart,G.S. and Costello,D.A.
  TITLE     Inhibition of Urea Transporter (UT)-B Modulates LPS-Induced
            Inflammatory Responses in BV2 Microglia and N2a Neuroblastoma Cells
  JOURNAL   Neurochem Res 46 (6), 1322-1329 (2021)
   PUBMED   33675462
  REMARK    GeneRIF: Inhibition of Urea Transporter (UT)-B Modulates
            LPS-Induced Inflammatory Responses in BV2 Microglia and N2a
            Neuroblastoma Cells.
REFERENCE   3  (bases 1 to 3675)
  AUTHORS   Kabutomori,J., Pina-Lopes,N. and Musa-Aziz,R.
  TITLE     Water transport mediated by murine urea transporters: implications
            for urine concentration mechanisms
  JOURNAL   Biol Open 9 (8) (2020)
   PUBMED   32661130
  REMARK    GeneRIF: Water transport mediated by murine urea transporters:
            implications for urine concentration mechanisms.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3675)
  AUTHORS   Wang,S., Liu,J., Cai,H., Liu,K., He,Y., Liu,S., Guo,Y. and Guo,L.
  TITLE     High salt diet elevates the mean arterial pressure of SLC14alpha1
            gene depletion mice
  JOURNAL   Life Sci 254, 117751 (2020)
   PUBMED   32387413
  REMARK    GeneRIF: results suggest that the depletion of SLC14alpha1 gene in
            mice may contribute to the HS-induced abnormality of sodium
            transportation in the CSF
REFERENCE   5  (bases 1 to 3675)
  AUTHORS   Kenig-Kozlovsky,Y., Scott,R.P., Onay,T., Carota,I.A., Thomson,B.R.,
            Gil,H.J., Ramirez,V., Yamaguchi,S., Tanna,C.E., Heinen,S., Wu,C.,
            Stan,R.V., Klein,J.D., Sands,J.M., Oliver,G. and Quaggin,S.E.
  TITLE     Ascending Vasa Recta Are Angiopoietin/Tie2-Dependent Lymphatic-Like
            Vessels
  JOURNAL   J Am Soc Nephrol 29 (4), 1097-1107 (2018)
   PUBMED   29237738
REFERENCE   6  (bases 1 to 3675)
  AUTHORS   Bankir,L., Chen,K. and Yang,B.
  TITLE     Lack of UT-B in vasa recta and red blood cells prevents
            urea-induced improvement of urinary concentrating ability
  JOURNAL   Am J Physiol Renal Physiol 286 (1), F144-F151 (2004)
   PUBMED   12965892
  REMARK    GeneRIF: UT-B-deficient mice reveal that countercurrent exchange of
            urea in renal medullary vessels and red blood cells accounts for a
            major part of the kidney's concentrating ability and for the
            adaptation of renal urea handling during a high-protein intake.
REFERENCE   7  (bases 1 to 3675)
  AUTHORS   Jung,J.Y., Madsen,K.M., Han,K.H., Yang,C.W., Knepper,M.A.,
            Sands,J.M. and Kim,J.
  TITLE     Expression of urea transporters in potassium-depleted mouse kidney
  JOURNAL   Am J Physiol Renal Physiol 285 (6), F1210-F1224 (2003)
   PUBMED   12952854
  REMARK    GeneRIF: potassium depletion is associated with reduced expression
            of UT-A1, UT-A3, and UT-B but increased expression of UT-A2
REFERENCE   8  (bases 1 to 3675)
  AUTHORS   Yang,B. and Verkman,A.S.
  TITLE     Analysis of double knockout mice lacking aquaporin-1 and urea
            transporter UT-B. Evidence for UT-B-facilitated water transport in
            erythrocytes
  JOURNAL   J Biol Chem 277 (39), 36782-36786 (2002)
   PUBMED   12133842
  REMARK    GeneRIF: UT-B has a role in urinary concentrating ability in mice
REFERENCE   9  (bases 1 to 3675)
  AUTHORS   Yang,B., Bankir,L., Gillespie,A., Epstein,C.J. and Verkman,A.S.
  TITLE     Urea-selective concentrating defect in transgenic mice lacking urea
            transporter UT-B
  JOURNAL   J Biol Chem 277 (12), 10633-10637 (2002)
   PUBMED   11792714
REFERENCE   10 (bases 1 to 3675)
  AUTHORS   Fenton,R.A., Hewitt,J.E., Howorth,A., Cottingham,C.A. and
            Smith,C.P.
  TITLE     The murine urea transporter genes Slc14a1 and Slc14a2 occur in
            tandem on chromosome 18
  JOURNAL   Cytogenet Cell Genet 87 (1-2), 95-96 (1999)
   PUBMED   10640821
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY231473.1, BC086673.1 and BC062816.1.
            
            On Jan 15, 2010 this sequence version replaced NM_028122.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (b) is shorter than isoform 1. Variants 2 and 3 encode the same
            isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC086673.1, SRR1660813.201774.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               BY231473.1         1-104
            105-3524            BC086673.1         1-3420
            3525-3525           BC062816.1         606-606
            3526-3675           BC086673.1         3421-3570
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 52.49 cM"
     gene            1..3675
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /note="solute carrier family 14 (urea transporter), member
                     1"
                     /db_xref="GeneID:108052"
                     /db_xref="MGI:MGI:1351654"
     exon            1..185
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            186..342
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1361
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /note="isoform b is encoded by transcript variant 2; urea
                     transporter 1; urea transporter B; urea transporter,
                     erythrocyte; solute carrier family 14 member 1"
                     /codon_start=1
                     /product="urea transporter 1 isoform b"
                     /protein_id="NP_082398.1"
                     /db_xref="CCDS:CCDS29360.1"
                     /db_xref="GeneID:108052"
                     /db_xref="MGI:MGI:1351654"
                     /translation="MEDSPTMVKVDRGENQILSCRGRRCGFKVLGYVTGDMKEFANWL
                     KDKPVVLQFMDWILRGISQVVFVSNPISGILILVGLLVQNPWWALCGCVGTVVSTLTA
                     LLLSQDRSAIAAGLQGYNATLVGILMAVFSNKGDYFWWLIFPVSAMSMTCPVFSSALS
                     SVLSKWDLPVFTLPFNMALSMYLSATGHYNTFFPSKLFTPVSSVPNITWSELSALELL
                     KSLPVGVGQIYGCDNPWTGGIFLCAILLSSPLMCLHAAIGSLLGVIAGLSLAAPFEDI
                     YFGLWGFNSSLACIAIGGMFMALTWQTHLLALACALFTAYFGACMAHLMAVVHLPACT
                     WSFCLATLLFLLLTTKNPNIYRMPLSKVTYSEENRIFYLQNKKRMVESPL"
     exon            343..532
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            533..661
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..854
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1002
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1137
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1187
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..3665
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3642..3647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /note="hexamer: AATAAA"
     polyA_site      3663
                     /gene="Slc14a1"
                     /gene_synonym="2610507K20Rik; 3021401A05Rik; UT-B; Utb1"
                     /note="major polyA site"
ORIGIN      
        1 gagtccgagg ctgaagtggg aggagaaaag caactggttc gcagacctga agagcagact
       61 gtgggtataa aagggtgagg cgcctcttct gcaccacctc tccacagcac taagcctgcc
      121 gtttaaaaga gcctggctgc tctttatctg gagccgcctt ctgccaccag gaagccagcc
      181 tgagcgagcc agaggaagag atagccatgg aagatagtcc caccatggtt aaagtagacc
      241 ggggtgaaaa ccagatttta tcatgccgcg ggagaaggtg tggcttcaaa gtacttggct
      301 acgtcacggg tgacatgaag gaattcgcca actggctgaa agacaaaccc gtggtgctcc
      361 agttcatgga ctggatactt cgtggcatat cccaggtggt gtttgtcagc aaccccatca
      421 gtggaatcct gattctggtg ggacttctgg tccagaaccc ctggtgggct ctctgtggct
      481 gtgtaggaac tgtggtctcc actctgacag ccctcttgct tagccaagac agatcggcga
      541 tagcagcggg gctccaaggt tacaatgcca ccctggtagg catcctcatg gctgtcttct
      601 caaacaaggg cgactatttc tggtggctga tattccctgt atctgctatg tctatgactt
      661 gcccggtttt ctcgagcgcg ttgagctccg tgctcagcaa gtgggacctg cccgtcttca
      721 ctctcccttt caacatggcg ttgtcgatgt acctgtcagc cacaggacac tacaatacgt
      781 ttttcccaag taaactcttc acacctgtca gctccgtgcc caacatcacg tggtctgagc
      841 tcagcgccct ggagctattg aagtctcttc cggtgggagt cggtcagata tatggctgtg
      901 acaacccgtg gacaggcggc attttcctat gtgctatcct gctctcctcc ccactcatgt
      961 gcctgcacgc tgctattgga tcgttgctgg gtgtcatcgc gggactcagt cttgcagctc
     1021 catttgaaga catctacttt gggctctggg gtttcaacag ctctctggcc tgcattgcaa
     1081 ttggagggat gttcatggca ctcacctggc agacccacct cctggctctt gcctgtgccc
     1141 tgttcactgc ctacttcgga gcctgtatgg cacacctgat ggctgtggtt cacctgccag
     1201 cttgtacctg gtccttctgt ttggccacac tactctttct cttgctgacc acgaaaaatc
     1261 ccaacatcta caggatgccc ctcagcaaag ttacctactc tgaggagaac cgcatcttct
     1321 acctccaaaa caagaaaagg atggttgaaa gccccctgta agagcagcgc cgcccaacag
     1381 ccatggtcat gagtcagttc taagtgcagc tccagttgac ttccaggctc tccacaattg
     1441 cttttcttct ctgggagcca cttccatact ctcggcgatc cattcttttg cttgcttctt
     1501 gtttttctct ttgactttat attatcaatc acatgagagc cgtgtccagt tgatgttctc
     1561 tggaatggaa tttaaacccc actgtgcact gggggtcggc aggagtatca ctaagactgc
     1621 atttagagag tcaaaatgtt ccgaccgaca gaaggaatgg aagcagcaac aattattgtt
     1681 atttgaagct atctttgatg tcagtattaa aagtcaagct ctgtaaacca tgtaagcctt
     1741 gcagaatcca cgggcacaga aacaaagcag cccagggttc tcggagaggc cttttgcttt
     1801 cagaatcagt gcaagttact cagagcaacc gcataacaac attgcagtgg ggcaggttga
     1861 ccagagcgct agtccccagc tctccagctg ggtggtgatg ttctcttaat aagaaacgtt
     1921 agttagcttt ttgttccaag ctgaccgagc tgtgggaaga actttgttgt ccaaaatagt
     1981 tttccactgc cacactcaga atttgcaaaa tctagtagag tttatttatt ctttatgaaa
     2041 atatttgccc actgatatat gtttgcagct agccagttct aagatattaa ataaaatgta
     2101 taaatgcata aaatactaag catacttgct tttgctttat atacttgctt tactatatat
     2161 tagtttggag ggattgtttg acatagggct gtctatgtag cccaggctag cctagaactc
     2221 agcatctccc tgtttccatt ccctaagcac cagagctgtg tgttgggttt ttaagtgctt
     2281 ataaggcaac ttcatcaacc tcgagagcct tttgaagcca cagttgaatg aatctgaggg
     2341 tatcatctaa acaccagcaa ctaagctgca ctcacagagc ctttcgagag ttctttagtt
     2401 tatttctttt ttcttttttc ttttcttttc ttttctttct ttcttttatt tgtttattta
     2461 atttcttttt tttaaggcat tatttcctgc acactaatgt tctggctagc aaactaatag
     2521 tatcagaacc accttacaga aatacagata gagaattctc tgggtttctt ttgtaaattt
     2581 ttaatttaaa agttgtggtt ttctaaaaaa caaaaacaaa agcaaacaaa caacaacaac
     2641 aacaaaaaac ccaaaccttc tacatccagt cttgctgaag cttcactgac cgcctgctgc
     2701 ctgtccagtg aaatttattt ctgggaggtt ggaaaagtga aaatttcaaa tcctaaatat
     2761 tttctttttc ctgacaagta tgttgcttgt gcccaaaatc ccagcactag ggagactgag
     2821 gtaggaggag cacaaactta ggaacatcca gcgtgggcta cacgaatggc tctatctcca
     2881 aaacccaacc gaacaaacac ataaacaagt cccccccccc cctctttcaa tagtcagtgt
     2941 taaccaggaa gtggcagcgg gggcaagctg atctctgagt tcaaggccag cctcgtctac
     3001 agagagagtt cctggacagc caagactaca ctgaaaaacc ctgcctcaaa caccaaaagc
     3061 agaaaatcgt gctgagaagg caatccgcag cttgctgaat ggctgaagtc agactgtagt
     3121 gcagatgcct tcagggcaca gccacattcc ctcttctcag gaaacaaatc gaaatctgtg
     3181 ataatttaaa atctcgtgac caaaaccctt agaagccatg gatttgcaac tgtcatggat
     3241 tcctgagtcg tcctatggaa agtctggatt tccagtgttt gagctgaaag tttcctgttt
     3301 ggagtttgtg gaataaacgt tgggtcagca agagtaatgt gcatgcgaag accccctcgg
     3361 aagtgaagat gctggatcgg agaagagtgg accaaagcat ctacaaaaca actgagcccc
     3421 agccagcagc aaagctctaa ctcacctaag gcaacattgt ctcagacctg aaaacaggga
     3481 caagtcgaaa ccttctacac tgttttattt gtgtaataaa tttttgtctt caaggtattt
     3541 ttgttactaa gtattatcat aaagttaaga gaaaactctt aggggacatt tgtactttcc
     3601 ccccctattg tctgtgtaac agactcctgt gtaaacatat gaataaatta tattaacagc
     3661 tcagaaaaaa aaaaa
//
