LOCUS       NM_001372402            3465 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 13 (sodium-dependent citrate
            transporter), member 5 (Slc13a5), transcript variant 2, mRNA.
ACCESSION   NM_001372402 XM_006533221
VERSION     NM_001372402.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Milosavljevic,S., Glinton,K.E., Li,X., Medeiros,C., Gillespie,P.,
            Seavitt,J.R., Graham,B.H. and Elsea,S.H.
  TITLE     Untargeted Metabolomics of Slc13a5 Deficiency Reveal Critical
            Liver-Brain Axis for Lipid Homeostasis
  JOURNAL   Metabolites 12 (4), 351 (2022)
   PUBMED   35448538
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3465)
  AUTHORS   Kopel,J.J., Bhutia,Y.D., Sivaprakasam,S. and Ganapathy,V.
  TITLE     Consequences of NaCT/SLC13A5/mINDY deficiency: good versus evil,
            separated only by the blood-brain barrier
  JOURNAL   Biochem J 478 (3), 463-486 (2021)
   PUBMED   33544126
  REMARK    GeneRIF: Consequences of NaCT/SLC13A5/mINDY deficiency: good versus
            evil, separated only by the blood-brain barrier.
            Review article
REFERENCE   4  (bases 1 to 3465)
  AUTHORS   Willmes,D.M., Daniels,M., Kurzbach,A., Lieske,S., Bechmann,N.,
            Schumann,T., Henke,C., El-Agroudy,N.N., Da Costa Goncalves,A.C.,
            Peitzsch,M., Hofmann,A., Kanczkowski,W., Kraker,K., Muller,D.N.,
            Morawietz,H., Deussen,A., Wagner,M., El-Armouche,A., Helfand,S.L.,
            Bornstein,S.R., de Cabo,R., Bernier,M., Eisenhofer,G., Tank,J.,
            Jordan,J. and Birkenfeld,A.L.
  TITLE     The longevity gene mIndy (I'm Not Dead, Yet) affects blood pressure
            through sympathoadrenal mechanisms
  JOURNAL   JCI Insight 6 (2), 136083 (2021)
   PUBMED   33491666
  REMARK    GeneRIF: The longevity gene mIndy (I'm Not Dead, Yet) affects blood
            pressure through sympathoadrenal mechanisms.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3465)
  AUTHORS   Fan,S.Z., Sung,C.W., Tsai,Y.H., Yeh,S.R., Lin,W.S. and Wang,P.Y.
  TITLE     Nervous System Deletion of Mammalian INDY in Mice Mimics Dietary
            Restriction-Induced Memory Enhancement
  JOURNAL   J Gerontol A Biol Sci Med Sci 76 (1), 50-56 (2021)
   PUBMED   32808644
  REMARK    GeneRIF: Nervous System Deletion of Mammalian INDY in Mice Mimics
            Dietary Restriction-Induced Memory Enhancement.
REFERENCE   6  (bases 1 to 3465)
  AUTHORS   Zwart,R., Peeva,P.M., Rong,J.X. and Sher,E.
  TITLE     Electrophysiological characterization of human and mouse
            sodium-dependent citrate transporters (NaCT/SLC13A5) reveal species
            differences with respect to substrate sensitivity and cation
            dependence
  JOURNAL   J Pharmacol Exp Ther 355 (2), 247-254 (2015)
   PUBMED   26324167
REFERENCE   7  (bases 1 to 3465)
  AUTHORS   Birkenfeld,A.L., Lee,H.Y., Guebre-Egziabher,F., Alves,T.C.,
            Jurczak,M.J., Jornayvaz,F.R., Zhang,D., Hsiao,J.J.,
            Martin-Montalvo,A., Fischer-Rosinsky,A., Spranger,J.,
            Pfeiffer,A.F., Jordan,J., Fromm,M.F., Konig,J., Lieske,S.,
            Carmean,C.M., Frederick,D.W., Weismann,D., Knauf,F., Irusta,P.M.,
            De Cabo,R., Helfand,S.L., Samuel,V.T. and Shulman,G.I.
  TITLE     Deletion of the mammalian INDY homolog mimics aspects of dietary
            restriction and protects against adiposity and insulin resistance
            in mice
  JOURNAL   Cell Metab 14 (2), 184-195 (2011)
   PUBMED   21803289
  REMARK    GeneRIF: Our studies demonstrate a profound effect of mIndy on
            mammalian energy metabolism.
            Erratum:[Cell Metab. 2011 Oct 5;14(4):567]
REFERENCE   8  (bases 1 to 3465)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
REFERENCE   9  (bases 1 to 3465)
  AUTHORS   Wada,M., Shimada,A. and Fujita,T.
  TITLE     Functional characterization of Na+ -coupled citrate transporter
            NaC2/NaCT expressed in primary cultures of neurons from mouse
            cerebral cortex
  JOURNAL   Brain Res 1081 (1), 92-100 (2006)
   PUBMED   16516867
  REMARK    GeneRIF: Na+ -coupled di/tricarboxylate transport system expressed
            in neurons as NaC2/NaCT, which can transport the tricarboxylate
            citrate as well as dicarboxylates such as succinate,
            alpha-ketoglutarate, and malate.
REFERENCE   10 (bases 1 to 3465)
  AUTHORS   Inoue,K., Fei,Y.J., Zhuang,L., Gopal,E., Miyauchi,S. and
            Ganapathy,V.
  TITLE     Functional features and genomic organization of mouse NaCT, a
            sodium-coupled transporter for tricarboxylic acid cycle
            intermediates
  JOURNAL   Biochem J 378 (Pt 3), 949-957 (2004)
   PUBMED   14656221
  REMARK    GeneRIF: Molecular cloning and transport properties of NaCT.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929071.6.
            
            On Aug 30, 2019 this sequence version replaced XM_006533221.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.4319825.1,
                                           SRR7652917.115988.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849376 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               AL929071.6         37198-37413         c
            217-337             AL929071.6         36716-36836         c
            338-466             AL929071.6         32275-32403         c
            467-603             AL929071.6         31865-32001         c
            604-791             AL929071.6         30447-30634         c
            792-960             AL929071.6         29210-29378         c
            961-1083            AL929071.6         27612-27734         c
            1084-1302           AL929071.6         23436-23654         c
            1303-1403           AL929071.6         22510-22610         c
            1404-1522           AL929071.6         20978-21096         c
            1523-1684           AL929071.6         17919-18080         c
            1685-1822           AL929071.6         15378-15515         c
            1823-3465           AL929071.6         12246-13888         c
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 43.95 cM"
     gene            1..3465
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /note="solute carrier family 13 (sodium-dependent citrate
                     transporter), member 5"
                     /db_xref="GeneID:237831"
                     /db_xref="MGI:MGI:3037150"
     exon            1..216
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..1954
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sodium-coupled citrate transporter; solute carrier family
                     13 member 5; novel solute carrier family 13
                     (sodium-dependent dicarboxylate transporter) (Slc13a2 or
                     3) member; sodium-dependent citrate transporter;
                     Na(+)/citrate cotransporter"
                     /codon_start=1
                     /product="Na(+)/citrate cotransporter isoform 2"
                     /protein_id="NP_001359331.1"
                     /db_xref="GeneID:237831"
                     /db_xref="MGI:MGI:3037150"
                     /translation="MRRRTLWREKPENPTGWCFSSGTFRSPGESSQSPFHAMDSAKTC
                     VTKFKSFAILLFTPILMLPLVILIPDKFARCAYVIVIMAVYWCTDVIPVAVTSLLPVL
                     LFPLLKVLDSKQVCIQYMKDTNMLFLGSLIVAVAVERWKLHKRVALRMLLFVGTKPSR
                     LMLGFMFVTAFLSMWISNTAATAMMIPIVEAMLQQMIAANTAVEASLGTLELLDKNKT
                     SELPGSQVVFEDPNVQEQEDEETKNMYKAMHLCVCYSASIGGTATLTGTGPNVVLLGQ
                     MQELFPDSKDVLNYASWFGFAFPNMVMMLVLAWLWLQCLYMRHNLKKTCICCGEKKRD
                     TEKIAYKVLNEEYQKLGSLSYPECNVLFCFTLLVILWFSRDPGFMPGWLSFAWVEGNT
                     VHITDATVAIFVAILLFIIPSQKPKFNFSSQTEEERKTPFYPPALLDWKVAQEKVPWD
                     IVLLLGGGFAMAKGCETSGLSKWMAAQMEPLRLVKPAVITLILSCLVAMTTECTSNVA
                     TTTLFLPIFASMARSIGIHPLYVMIPCTMSASLAFMLPVATPPNAIVFAYGHLRVVDM
                     MKTGLIMNFVGILSVFLSVNTWGRAMFNLDNFPDWANSTSVNT"
     exon            217..337
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            338..466
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            467..603
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            604..791
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            792..960
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1083
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1302
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1403
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1522
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1684
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1822
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..3465
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3443..3448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /note="hexamer: AATAAA"
     polyA_site      3465
                     /gene="Slc13a5"
                     /gene_synonym="Indy; mINDY; NaC2/NaCT; Nact"
                     /note="major polyA site"
ORIGIN      
        1 aaatgggttc tatgtctaga gtcgggctaa cgctaggagg aagccagcgg atcttggagg
       61 tgagaagacc gcggtggcgc agggctcggc catcgcctct aggagacgcc cctccgggat
      121 agggatgaga aggagaacgc tttggagaga gaagcctgag aatcctacag gctggtgttt
      181 ttcatctggg acattccgga gtccaggaga gagcagtcag tctccctttc acgcgatgga
      241 ttcggcgaag acttgtgtga ccaagttcaa gtcctttgcg attttgctct tcaccccgat
      301 cctgatgctt ccactcgtca ttctgatacc tgacaagttt gccaggtgtg cctatgttat
      361 agtcattatg gctgtctact ggtgcacaga tgtcatccca gtggctgtta cctccctcct
      421 gcctgtctta ctcttcccac ttttgaaggt tctggactcc aagcaggtat gtatccaata
      481 catgaaggac accaacatgc tgttcctggg cagtctcatt gtggctgtgg ctgtggaacg
      541 ctggaaactt cataagaggg ttgccctgag aatgctgctc tttgtgggga ccaagccctc
      601 acggctgatg ctgggcttta tgtttgtcac ggccttcctg tccatgtgga tcagcaatac
      661 tgccgccaca gccatgatga tacccattgt ggaggccatg ctgcagcaaa tgatagccgc
      721 caatacagct gtggaggcca gcctggggac actggagctg ctggacaaga acaagaccag
      781 cgagttgcca ggaagccagg tggtatttga agaccccaat gtgcaggagc aggaagacga
      841 agaaacaaag aatatgtaca aggctatgca cctatgtgtt tgctattcag ccagcattgg
      901 gggtacagcc accttgaccg ggacgggacc caacgtggtg ctcctgggcc agatgcagga
      961 attgtttcct gacagtaaag atgtcctgaa ctatgcatct tggtttggat ttgccttccc
     1021 caacatggtg atgatgctgg tgctggcctg gctgtggctc cagtgcttgt acatgagaca
     1081 caatttaaaa aaaacttgca tctgctgtgg ggagaagaag agggacaccg agaagattgc
     1141 ctacaaagtg ctgaacgagg agtaccagaa gctggggtcc ttgagctacc ctgaatgcaa
     1201 cgtgctcttt tgcttcaccc tacttgtcat cctgtggttc tcccgagacc ccggcttcat
     1261 gcctggctgg ctgtcattcg cctgggtcga gggaaacacc gttcatatca cagatgccac
     1321 agtggccatc tttgtggcca ttttgctttt catcatacct tcacaaaagc ccaagttcaa
     1381 cttcagcagc cagactgagg aagaaaggaa aactccgttc taccccccag cactgctgga
     1441 ttggaaagtc gcccaagaga aagtgccctg ggacatcgtg ctgctcctgg ggggagggtt
     1501 tgctatggca aaaggatgtg agacgtcagg gctctcgaag tggatggcag cacagatgga
     1561 acccttgaga ttagtgaaac ctgctgtcat taccttgatc ttgtcctgtc ttgttgcaat
     1621 gaccacagag tgcacaagta acgtggccac taccaccctg ttcctgccta tctttgcctc
     1681 catggctcgt tccattggta tccatcctct gtatgtcatg attccctgta ccatgagtgc
     1741 ttcacttgcc ttcatgttgc ctgtggccac cccaccgaat gccatcgtgt ttgcctacgg
     1801 acacctcaga gttgttgaca tgatgaaaac aggattgata atgaacttcg ttggaatcct
     1861 atctgtgttt ctgtcagtca acacctgggg tcgggctatg tttaacttgg ataacttccc
     1921 cgactgggca aattcaacaa gtgttaacac ttaggaagag ccgcaagagc acaagcatgc
     1981 cccccccacc ccaacccttt tgaggactat gaaccttctg gcacaccttg cacagagcac
     2041 tggtgctcac actctagtgt gatccaatga tgtcaacacc ccaagaagat ctacccaact
     2101 atatcaagtt cagagatggc aatggatgat gggaagataa gctcagaagg gaaaggaacc
     2161 cctttgagag gtcgcgaggc ccatctttac taggaccctt ccatctctcc tgggacaggc
     2221 aggaaccaga gggaccaggg ctcaaatctt gtactatgtg gctttgacag actactggct
     2281 ccatgctggg ctcccggttc tcacatgcct gctcccacgg tctccacacg ggggggtggg
     2341 gggggagcgg attgccaggc tgggagcccc gtcctggtgc ctcttctgga tgtccccaga
     2401 tcatcatatc tatcaccacg agagaacttc ccatccaggg cagaactctg atcttaaaca
     2461 cttcccactg ccagagtgag agtgggagtc atggcctcct gaaaacttgg actctgcatg
     2521 gtgccaccac caaccattga gtagtctgag gtgctaacat caggccctcc tgctcactcc
     2581 ttggctgacc tgccccccta cttgcctcat ttcatatttc atattcattt catatttcat
     2641 attcattaac agtttgagct cccttcccag ctccccagta agccctcctt cagcctccag
     2701 atccccaccc ccctagctga ggctccccca gaaaacccct ggtttttttt ttttccaggg
     2761 agctcagttt gatcattctg ggaattcttg tcctgccagc ctggtgcggc tgggatcaca
     2821 cttcaaggat ggacagtggt gtctctttgg gagatggtgt tcaagtggga tacctaagtc
     2881 ttcccgtggc tagatccaga gcagattcag ccggctcatt tccaagggct gggccactgc
     2941 acagtttgtc tgtccccgtt ggactcttga gctcctctgg gaaggccttt cccttgccgg
     3001 ctgtgcattg tggccaggac tggtagcaac agagcatggc tgagaaggag cagcgtgctt
     3061 cttggtgtct gagcagacag accctgccag agtaaactgg ctgaggggag tgtccatcat
     3121 atgaaaggga gacaggagag gaagctctcc gttgtctttc agtgataccc tcttccttgc
     3181 ttaatttgct ctgaaaagaa gcacagttgg gtagacgagg cccgtggatg tcattttttt
     3241 ttttctgggc agaagacagt atggggaccc aagaagttcc catcaaagac caagtatgtg
     3301 cctggctcct taggagggat gactcctgac cacactgttg aaggcttgct cttgtcaaat
     3361 actctgtctg ctatggaaag ttccagtgtg ctgactggtc tgtgattttg taccttgtga
     3421 aggagacaga gggaggcaga tgaataaaca gttgctctct gtcca
//
