LOCUS       NM_134251               3303 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus solute carrier family 12 (potassium/chloride
            transporters), member 8 (Slc12a8), transcript variant 1, mRNA.
ACCESSION   NM_134251
VERSION     NM_134251.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ito,N., Takatsu,A., Ito,H., Koike,Y., Yoshioka,K., Kamei,Y. and
            Imai,S.I.
  TITLE     Slc12a8 in the lateral hypothalamus maintains energy metabolism and
            skeletal muscle functions during aging
  JOURNAL   Cell Rep 40 (4), 111131 (2022)
   PUBMED   35905718
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Grozio,A., Mills,K.F., Yoshino,J., Bruzzone,S., Sociali,G.,
            Tokizane,K., Lei,H.C., Cunningham,R., Sasaki,Y., Migaud,M.E. and
            Imai,S.I.
  TITLE     Slc12a8 is a nicotinamide mononucleotide transporter
  JOURNAL   Nat Metab 1 (1), 47-57 (2019)
   PUBMED   31131364
  REMARK    Erratum:[Nat Metab. 2019 Jul;1(7):743. doi:
            10.1038/s42255-019-0088-x.. PMID: 32694647]
REFERENCE   3  (bases 1 to 3303)
  AUTHORS   Blaesse,P., Airaksinen,M.S., Rivera,C. and Kaila,K.
  TITLE     Cation-chloride cotransporters and neuronal function
  JOURNAL   Neuron 61 (6), 820-838 (2009)
   PUBMED   19323993
  REMARK    Review article
REFERENCE   4  (bases 1 to 3303)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127412.4, BU921470.1, AF319951.1, AK165080.1 and AC121570.4.
            
            On Jun 20, 2019 this sequence version replaced NM_134251.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF319951.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AC127412.4         98258-98342
            86-517              BU921470.1         169-600
            518-681             AF319951.1         378-541
            682-682             AC127412.4         131106-131106
            683-841             AF319951.1         543-701
            842-842             AC127412.4         170403-170403
            843-2568            AF319951.1         703-2428
            2569-3300           AK165080.1         1942-2673
            3301-3303           AC121570.4         152428-152430       c
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 24.12 cM"
     gene            1..3303
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /note="solute carrier family 12 (potassium/chloride
                     transporters), member 8"
                     /db_xref="GeneID:171286"
                     /db_xref="MGI:MGI:2443672"
     exon            1..182
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2252
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     solute carrier family 12 member 8; cation-chloride
                     cotransporter 9"
                     /codon_start=1
                     /product="solute carrier family 12 member 8 isoform 1"
                     /protein_id="NP_599012.2"
                     /db_xref="CCDS:CCDS49834.1"
                     /db_xref="GeneID:171286"
                     /db_xref="MGI:MGI:2443672"
                     /translation="MAQRSPQELFHEAAQQGILAQPQPWWKIQLFMWEPVLFGTWDGV
                     FTSCMINIFGVVLFLRTGWLVGNTGVLLGLLLVSFVVLVALITVLSGIGVAEHGGISS
                     GGVYSMISSVLGGQMGGTVGLLYVFGQCVAGAMYITGFAESISDLLGLGDIWAVRGIS
                     VAVLLALLGINLAGVKWIIRLQLLLLLLLAVSTLDFVVGSFTHLDPEHGFIGYSPELL
                     QSNILPEYSPGESFFTVFGVFFPAATGVMAGFNMGGDLRDPADSVPLGSLAAVGVSWF
                     LYIIFAFLLGAVCTREALRSDFLIAEKVSLVGFLFLLGLYISSLASCMGGLYGAPRIL
                     QCIAQDKVIPALAFLANGKGPNKTPVAAICLTSLVTMAFVLVGQVNVLAPVVTINFML
                     TYIMVDYSYFALSMAHCGLAPSPEPVPRQGPDTLHCSEHLLQDRAPSYGSDVPARSLS
                     EGTLLEFTKDMDQFLQPIEELESRQLGSREGNNPKNQKRKGKKGAKQTLQDSFLLDPG
                     SPLSFPTRTSERLSVAFCGEQESYQKQQTSRSESHDHLVPDLRNQPRVNREDFFLKCR
                     LQEQEIQRRPSVFYACMCNPWVSLLGALASLLIMFVIQWLYTLASMGVAALVYFYIGQ
                     ASPGLYLGSASNFSFFQWMKSFLVPSCRSLRSAQEQIILAPSPAKVDMAMTQLTQDNA
                     DFATRDRYHHSSFLSREQLMPPY"
     exon            183..329
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            330..521
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            522..753
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            754..867
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            868..955
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1043
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1190
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1812
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1910
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2028
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2089
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..3303
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2550..2555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2573
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /note="major polyA site"
     regulatory      3278..3283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3303
                     /gene="Slc12a8"
                     /gene_synonym="CCC9; E330020C02Rik"
ORIGIN      
        1 ctcagtgcag aggaagctgg acaggcaaga agcagcaagg gagggaggga gggcctggcc
       61 tgacagagtg atgtttgctt tccagagtgg tctccctatc tagataaaag tatcagaacc
      121 tcacttcctg gagaatggcc cagaggtctc cgcaagaact cttccacgag gcagcccagc
      181 agggcatcct ggcccagccc cagccctggt ggaagatcca gctgttcatg tgggagccgg
      241 tgctgtttgg gacctgggat ggtgtgttca catcctgcat gatcaacatt tttggcgttg
      301 tccttttctt gaggaccggc tggctggtgg gaaacacagg tgtgctcctg ggcttgctcc
      361 tggtgtcctt cgtcgtcctc gtggccctca tcaccgtgct gtcgggcatt ggtgtcgcag
      421 agcatggcgg gatcagcagt ggcggtgtct actccatgat ctcctcggtg cttggtgggc
      481 agatgggagg cactgtgggg ctgctctatg tatttggaca gtgtgttgca ggtgctatgt
      541 acatcaccgg ctttgcggag tccatctcag atctgctggg acttggggac atctgggcag
      601 tgcgtggaat ttcagttgct gtgcttctgg ctttgctggg catcaacctg gcaggtgtca
      661 agtggattat ccgcctccag ctgctgctgc tgctcctgct ggctgtctcg accctggact
      721 ttgtggtggg ctctttcacc cacctggacc cagaacatgg ctttattggc tactccccag
      781 aactgctaca gagcaacatt ctgccagagt acagccccgg ggagtcattc ttcactgtgt
      841 ttggggtgtt cttccctgca gctacaggag tcatggctgg cttcaacatg ggaggagacc
      901 tgagagaccc tgctgacagt gtccccttag gctccctagc agctgttggc gtctcgtggt
      961 ttctctacat catctttgcc ttcctgcttg gtgccgtctg tacccgagag gccctccgct
     1021 ctgacttcct gatagctgaa aaggtgtctc tggttggttt cctcttccta ttgggcctgt
     1081 acatctcatc cctggcttcc tgtatggggg gactctatgg cgcaccccgg atcctgcagt
     1141 gcatcgccca ggacaaagtc atccctgcac tcgcctttct ggcgaatggg aaagggccaa
     1201 ataaaacacc ggtagcagcc atctgcctga ccagcttggt gaccatggcc tttgtcctgg
     1261 tgggtcaggt gaatgttctg gcgcccgttg tcaccatcaa tttcatgctg acctacatca
     1321 tggtggacta ctcttacttc gccctctcca tggctcactg tggcctcgcc ccatctcctg
     1381 agcccgtccc cagacaaggc ccagatactc tgcactgctc tgagcacctg ctccaggaca
     1441 gggctcccag ctacggctct gatgtccctg ccagaagcct ctctgagggc accctgctgg
     1501 agttcaccaa ggacatggat cagttcctcc agccaataga ggaactggag agtcgtcagc
     1561 ttgggtcaag agaaggaaac aacccaaaga atcagaagcg caagggtaag aaaggcgcca
     1621 agcaaaccct acaagatagc ttcctcttgg accctgggtc tcctttgtcc tttcctacga
     1681 ggacttctga gaggttgtct gttgccttct gtggggagca agagtcctat cagaagcagc
     1741 agacttctag gagtgaatca catgaccatc ttgttcctga tctacgcaac cagcctagag
     1801 tgaacagaga agatttcttt ctgaaatgca gacttcagga acaagagatc cagagaagac
     1861 caagtgtttt ctatgcttgc atgtgtaacc cctgggtctc cctgttaggg gctcttgcat
     1921 ccctgctcat catgtttgtg atccagtggc tctataccct agctagtatg ggtgttgctg
     1981 cccttgtgta tttctacatt ggccaggcaa gtccaggcct ttacctcgga tcagcatcaa
     2041 acttcagctt tttccaatgg atgaagtcct tcttggtccc ctcctgcagg agcctgaggt
     2101 ccgcccagga gcaaatcatc ttggcgccat caccagccaa ggttgacatg gcaatgactc
     2161 agcttaccca ggacaatgca gacttcgcca cccgagatcg ttaccaccac tcctccttcc
     2221 tgagccggga gcagttgatg cctccctact aggacctcgc tggaccccac ctttctagaa
     2281 gccgctgtgg agatggggga cccaagcctc agaacctttg gaagttgctt ctaccaataa
     2341 gaaaacccaa gactggtctt cccaccacag ccttggacgc tggaaatcac attccggtct
     2401 gaaatggcat gtctagccag atactaggta tgactatggc ttccacgtgt gcccaacctg
     2461 aagagtccac aggcaccagg atgatcaggt gacaccaaga caaccaaatg tggagaatca
     2521 aaacgagatt tcttattcta aggggccaaa ataaacactt ctttttaatt ttattgtttt
     2581 attttttatt atttttaaag aattatatat atatacatat actgtagcta ttttcagaca
     2641 gaaaatagca tcaccagaag agggcatcgg atcccattac agatggttgt gagccaccat
     2701 gtggttgctg ggaattgaac tcaggatctc tggaagaaca gtcagtgctc ctaactgctg
     2761 agccatctct ccagcaccct cccctgtttt atttattttg taatgtatac taactacact
     2821 cacatggttc ccaagccaca gagtatagaa acccccttct ccctgtttct catctgtttg
     2881 gtgtccgtcc cccatcacag gggcaaccac ttctcatgca aacatcagcc acctctttac
     2941 agacccctcc catctgcaaa tgcagctctg tttacaatgc actacatccc gctcttctcc
     3001 cttaatctgc catagaagca tttccatgaa gatgcttaca cagtttcctc cttatgtctc
     3061 tatgtctgaa tgtgccttaa tttatatact cagcctcacg aggacggcat ttagcttcat
     3121 tctgatattt tgctattaca gccgcttctg caaagagtca ctgtgtttgt gtgcacagtc
     3181 gtgaaggata cctgaagata aattctcaga aggagaatta cagggtgact ggggatgtac
     3241 atttgtaagc tgttactgtt tggagaaatg ctttttaatt aaaaagtcaa tcaaatacca
     3301 tga
//
