LOCUS       NM_133649               6631 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus solute carrier family 12, member 6 (Slc12a6),
            transcript variant 2, mRNA.
ACCESSION   NM_133649
VERSION     NM_133649.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ferdaus,M.Z., Terker,A.S., Koumangoye,R. and Delpire,E.
  TITLE     KCC3a, a Strong Candidate Pathway for K+ Loss in Alkalemia
  JOURNAL   Front Cell Dev Biol 10, 931326 (2022)
   PUBMED   35874803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Shmukler,B.E., Rivera,A., Nishimura,K., Hsu,A., Wohlgemuth,J.G.,
            Dlott,J.S., Michael Snyder,L., Brugnara,C. and Alper,S.L.
  TITLE     Erythroid-specific inactivation of Slc12a6/Kcc3 by EpoR
            promoter-driven Cre expression reduces K-Cl cotransport activity in
            mouse erythrocytes
  JOURNAL   Physiol Rep 10 (5), e15186 (2022)
   PUBMED   35274823
  REMARK    GeneRIF: Erythroid-specific inactivation of Slc12a6/Kcc3 by EpoR
            promoter-driven Cre expression reduces K-Cl cotransport activity in
            mouse erythrocytes.
REFERENCE   3  (bases 1 to 6631)
  AUTHORS   Lim,W.M., Chin,E.W.M., Tang,B.L., Chen,T. and Goh,E.L.K.
  TITLE     WNK3 Maintains the GABAergic Inhibitory Tone, Synaptic Excitation
            and Neuronal Excitability via Regulation of KCC2 Cotransporter in
            Mature Neurons
  JOURNAL   Front Mol Neurosci 14, 762142 (2021)
   PUBMED   34858138
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6631)
  AUTHORS   Flores,B. and Delpire,E.
  TITLE     Temporal manipulation of KCC3 expression in juvenile or adult mice
            suggests irreversible developmental deficit in hereditary motor
            sensory neuropathy with agenesis of the corpus callosum
  JOURNAL   Am J Physiol Cell Physiol 320 (5), C722-C730 (2021)
   PUBMED   33596149
  REMARK    GeneRIF: Temporal manipulation of KCC3 expression in juvenile or
            adult mice suggests irreversible developmental deficit in
            hereditary motor sensory neuropathy with agenesis of the corpus
            callosum.
REFERENCE   5  (bases 1 to 6631)
  AUTHORS   Flores,B. and Delpire,E.
  TITLE     Osmotic Response of Dorsal Root Ganglion Neurons Expressing
            Wild-Type and Mutant KCC3 Transporters
  JOURNAL   Cell Physiol Biochem 54 (4), 577-590 (2020)
   PUBMED   32506846
  REMARK    GeneRIF: Osmotic Response of Dorsal Root Ganglion Neurons
            Expressing Wild-Type and Mutant KCC3 Transporters.
REFERENCE   6  (bases 1 to 6631)
  AUTHORS   Piechotta,K., Lu,J. and Delpire,E.
  TITLE     Cation chloride cotransporters interact with the stress-related
            kinases Ste20-related proline-alanine-rich kinase (SPAK) and
            oxidative stress response 1 (OSR1)
  JOURNAL   J Biol Chem 277 (52), 50812-50819 (2002)
   PUBMED   12386165
  REMARK    GeneRIF: Ste20-related proline-alanine-rich kinase (SPAK) and
            oxidative stress response 1 (OSR1) with the cotransporters KCC3,
            NKCC1, and NKCC2 but not KCC1 and KCC4
REFERENCE   7  (bases 1 to 6631)
  AUTHORS   Howard,H.C., Mount,D.B., Rochefort,D., Byun,N., Dupre,N., Lu,J.,
            Fan,X., Song,L., Riviere,J.B., Prevost,C., Horst,J., Simonati,A.,
            Lemcke,B., Welch,R., England,R., Zhan,F.Q., Mercado,A.,
            Siesser,W.B., George,A.L. Jr., McDonald,M.P., Bouchard,J.P.,
            Mathieu,J., Delpire,E. and Rouleau,G.A.
  TITLE     The K-Cl cotransporter KCC3 is mutant in a severe peripheral
            neuropathy associated with agenesis of the corpus callosum
  JOURNAL   Nat Genet 32 (3), 384-392 (2002)
   PUBMED   12368912
  REMARK    GeneRIF: SLC12A6 has a role in the development and maintenance of
            the nervous system
            Erratum:[Nat Genet 2002 Dec;32(4):681]
REFERENCE   8  (bases 1 to 6631)
  AUTHORS   Hubner,C.A., Stein,V., Hermans-Borgmeyer,I., Meyer,T., Ballanyi,K.
            and Jentsch,T.J.
  TITLE     Disruption of KCC2 reveals an essential role of K-Cl cotransport
            already in early synaptic inhibition
  JOURNAL   Neuron 30 (2), 515-524 (2001)
   PUBMED   11395011
REFERENCE   9  (bases 1 to 6631)
  AUTHORS   Pearson,M.M., Lu,J., Mount,D.B. and Delpire,E.
  TITLE     Localization of the K(+)-Cl(-) cotransporter, KCC3, in the central
            and peripheral nervous systems: expression in the choroid plexus,
            large neurons and white matter tracts
  JOURNAL   Neuroscience 103 (2), 481-491 (2001)
   PUBMED   11246162
REFERENCE   10 (bases 1 to 6631)
  AUTHORS   Mount,D.B., Mercado,A., Song,L., Xu,J., George,A.L. Jr., Delpire,E.
            and Gamba,G.
  TITLE     Cloning and characterization of KCC3 and KCC4, new members of the
            cation-chloride cotransporter gene family
  JOURNAL   J Biol Chem 274 (23), 16355-16362 (1999)
   PUBMED   10347194
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133006.1, BC036323.1 and BC051061.1.
            
            On Sep 5, 2007 this sequence version replaced NM_133649.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF211854.1, SRR10223969.2759.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3857              AK133006.1         491-4347
            3858-6604           BC036323.1         1430-4176
            6605-6631           BC051061.1         4452-4478
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 56.99 cM"
     gene            1..6631
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /note="solute carrier family 12, member 6"
                     /db_xref="GeneID:107723"
                     /db_xref="MGI:MGI:2135960"
     exon            1..779
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             509..3961
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /note="isoform 2 is encoded by transcript variant 2; K-Cl
                     cotransporter 3; electroneutral potassium-chloride
                     cotransporter 3"
                     /codon_start=1
                     /product="solute carrier family 12 member 6 isoform 2"
                     /protein_id="NP_598410.2"
                     /db_xref="CCDS:CCDS16551.1"
                     /db_xref="GeneID:107723"
                     /db_xref="MGI:MGI:2135960"
                     /translation="MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVR
                     FSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDPSQNSITGEHS
                     QLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLT
                     QGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGIL
                     QAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGT
                     TFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVF
                     IGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSK
                     TKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSN
                     YLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGD
                     LKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSW
                     PSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALL
                     LTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWA
                     LSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARF
                     ALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVG
                     NFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTV
                     VMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVW
                     WIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAE
                     VEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSI
                     GSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAV
                     KLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVI
                     TIYS"
     exon            780..824
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            825..919
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1051
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1198
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1253
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1384
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1626
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1841
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..2000
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2099
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2157
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2332
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2451
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2550
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2670
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..2775
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2776..2944
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3140
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3310
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3311..3442
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3443..3550
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3551..3735
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3869
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     exon            3870..6617
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5939..5944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /note="hexamer: AATAAA"
     polyA_site      5962
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /note="major polyA site"
     regulatory      6435..6440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
                     /note="hexamer: ATTAAA"
     polyA_site      6471
                     /gene="Slc12a6"
                     /gene_synonym="9530023I19Rik; gaxp; KCC3"
ORIGIN      
        1 caacaggtgg ctggtgcaca cctttgcgca atctgatcca cgctcttata cgcctgataa
       61 gggtgggcct gcatgctccg tcctcagcta gaaccgtggg acactcggca gataaaggac
      121 taactacctc atccggaccc tgggggttga gcagagggag gcgtcaccag ctgctgtgag
      181 atcatggcac ggagccctca gccctggacc aggggagata gctaggattc tgaaagaacc
      241 aagttataca ggattagcat cgttttgttc ttattttgtt ttctcgaaaa ttatttttca
      301 gttactggtg gggcacttta taaaacagct ggcttgaatt ctatacacgg attcttaatt
      361 gggcctttgt gggctgtaaa tcagggtaat tgagggtttt tggttttttt tccccttcta
      421 tttttgcaat cagaagtagc tagtgtagga ggaagagttt ttgtgagctt ttcctttttt
      481 ctttgtcaaa aaggaaaggg gggggaaaat gcatccacca gaagccacca ccaagatgtc
      541 ctcagttcgg ttcatggtga caccaactaa gattgatgac attccaggtt tgtcagacac
      601 cagcccggac ctcagctctc ggtctagttc tcgagtaaga tttagctccc gagaaagtgt
      661 gccagaaaca agccgtagtg agcctatgag cgaactgtct ggggctacta cttctctggc
      721 aactgttgcc ctagatcctt ccagtgaccg gacttctaat ccccaggatg ttacggagga
      781 cccgagtcag aactccatca caggggagca cagccagctg ttagatgacg gccataaaaa
      841 agcccgaaat gcttatctca ataattccaa ctatgaagaa ggagacgaat attttgataa
      901 aaatttggca ctctttgagg aagaaatgga caccagacca aaggtgtctt ctctcctcaa
      961 ccgcatggcc aactatacaa atctgacaca aggagcaaag gaacatgaag aggcagagaa
     1021 catcactgaa gggaaaaaga agcctaccaa gagcccccaa atgggtactt tcatgggtgt
     1081 ctacctccca tgtctacaga acatctttgg agtgatcctc ttcctgcgtc ttacctgggt
     1141 agtgggaaca gctggaatcc ttcaggcctt tgcaattgtc ctcatctgct gctgctgtac
     1201 aatgttaact gccatctcca tgagcgccat cgccactaac ggagtggtgc cagctggggg
     1261 ctcatacttc atgatttcca gagccctggg cccagagttt ggcggggctg taggcctctg
     1321 cttttatctt ggcaccacat ttgcagcagc catgtatatt cttggtgcca ttgaaatctt
     1381 tctggtatac attgtccccc gagctgccat ctttcggagt gacgatgcac tcaaggagtc
     1441 agcagctatg ctgaacaaca tgcgcgtcta tggtacagcc ttcttggtcc tcatggtctt
     1501 ggtggtattc atcggcgtac gctatgtgaa taagtttgcc tcactcttcc tggcctgtgt
     1561 aattgtgtcg atcttggcta tctatgctgg tgccatcaag tcttcctttg ctccaccaca
     1621 cttcccggtc tgtatgctgg gcaaccgtac cctgtcatca agacaccttg acatttgctc
     1681 taagaccaag gaggttgaca acatgacagt accatcaaag ttatggggat tcttctgcaa
     1741 ctcgagtcag ttctttaatg ccacctgtga tgagtacttt gttcacaata acgtcatctc
     1801 aatccaaggc attccagggt tggctagtgg tatcattact gaaaatcttt ggagtaatta
     1861 tttaccaaag ggtgagataa ttgaaaagcc atcagccaag tcatctgatg tcttaggcaa
     1921 cttaaaccat gaatatgttc ttgctgatat caccacctcc ttcactctgc tggtggggat
     1981 cttctttccc tcggtcacag gtatcatggc tgggtcaaac agatctggag atctgaaaga
     2041 tgcccagaag tctattccca ttgggaccat ccttgccatc ctgaccacat cctttgtgta
     2101 tttaagcaat gttgtccttt ttggtgcatg tattgaagga gtcgttctca gagacaaatt
     2161 tggggatgct gtaaaaggga atttggttgt aggcacctta tcctggccat ccccgtgggt
     2221 gatcgttatt ggctccttct tttcaacatg tggggctggg ctgcagagcc tcacaggtgc
     2281 gcctcggctg ctgcaggcta tcgccaagga taacatcata cctttcctta gggtttttgg
     2341 tcacagcaaa gctaatgggg aacctacctg ggctttactt ctaactgctg ccatagcaga
     2401 gctgggaatt ctcatcgcct ccctggatct cgtggcccca attctttcca tgttttttct
     2461 catgtgttac ctctttgtga acttggcttg tgccttgcaa acattgctgc gaacccccaa
     2521 ctggaggcct cgattccgct attatcactg ggccctctct ttcatgggaa tgagtatctg
     2581 tctagctctg atgttcattt cttcttggta ttatgccatt gtagctatgg taatagctgg
     2641 catgatctac aagtacattg aatatcaagg ggctgagaaa gaatgggggg atggtatccg
     2701 tgggctgtcg ctcagtgcag cccgcttcgc tttgctccgt ctagaggaag gacctcctca
     2761 cactaaaaac tggaggcctc agctgctcgt cctactgaag ctggatgaag atttacacgt
     2821 caagcaccct cgcctcctca cctttgcctc ccagctcaag gcaggaaagg gactcacgat
     2881 tgtgggctct gtcatcgtgg ggaacttctt ggagaactat ggtgacgcgc tcgcggcaga
     2941 gcagaccatt aagcacctaa tggaggcaga aaaggtaaaa ggattctgcc aattggtggt
     3001 ggctgccaag ctgaaagagg gcatttcaca cctcatccag tcctgtggcc tcggaggcat
     3061 gaaacacaac acagtggtga tgggctggcc caatggctgg cgtcagagtg aagatgctcg
     3121 cgcttggaag actttcattg gcacagtacg agtgacaact gctgcccatc tagccctgct
     3181 ggtggctaaa aatgtctcct tctttcccag caatgtggag cagttttctg agggcaacat
     3241 tgatgtgtgg tggattgtgc atgatggggg catgctcatg ctattaccgt tcctgctgaa
     3301 acagcacaag gtttggcgga aatgcagcat acggatcttc acagtagccc aactagaaga
     3361 caacagtatc cagatgaaga aggatctggc cacctttctg taccacctgc gcattgaggc
     3421 agaagtggaa gtggtggaga tgcacgacag tgacatatct gcctatacat atgagcgcac
     3481 cctgatgatg gagcagaggt cccagatgct tcggcatatg cggctgtcca aaacagagcg
     3541 agacagggag gcacagctgg tgaaagatcg aaactcaatg ctacgcttga ccagcattgg
     3601 ctctgatgag gacgaagaga cagaaacgta ccaggagaag gtgcacatga cttggaccaa
     3661 ggataaatac atggcatccc gggggcaaaa ggtcaagtca atggaaggat tccaggacct
     3721 acttaatatg cgtccggacc agtccaacgt gagacggatg catacagcag tgaagctcaa
     3781 tgaagttata gtcaacaagt ctcatgaagc aaagctggtt ttgttgaata tgccaggacc
     3841 accccggaac cctgaaggtg atgaaaacta catggaattt ctagaagtgc tcactgaggg
     3901 attagaacga gtccttcttg tccggggtgg tggcagtgag gtcatcacca tttactcata
     3961 atcagctgtg ggagattctg cgtggtccga cttccctaag actcatcgtc catggagatg
     4021 atagctcttt cctaccactc ccactctatt cttgcagagc tgagccccat ctatgctctt
     4081 ggctgcaaca tgatctgcca tcccagcaga aacaaataat ccctcaacag aagaatggtc
     4141 aagtcctaaa agctatttct atggcaggag aaggcaagtc aaaattaaca agctaagcca
     4201 aagggaacct tttggcacac agaaggtaat gagttagtaa aacatatgct agaaatgtaa
     4261 ctggcaacag aaagcaatgt tcatatctaa cattcaggac gagtttcaga agacacgggt
     4321 cagtgagcac cagaaagtta acaggcagtt aagcttcacc ttagtaaaaa atgagttccc
     4381 attatagaga cagcctgcag cagccaggat ggaaacagga tactgatata taaaacctga
     4441 gacatagctg cagccactac ttcttcctca tggtttattg tctgtactgt accactgtac
     4501 attttaactg tctttgtttt aaaacccatt ctgtggttat aatgcagaca gtagtgttac
     4561 tatagaaatg tttttttgaa aggctagaaa tgttttttca ggttttcaaa tgctgaacta
     4621 aggggattcc aaagagtgcg tattaaaata tatagaaaca cagtggttag ctatgcaaca
     4681 taaagcacac aagctattat cttaacaaat gtgggttgtt atgtagttaa ccagaagggt
     4741 ctcttctact cttagtaggc attggtctta aaaaccaaac aaaccaaacc tgtaaggaag
     4801 agacgccagg gctgtgtgat gccacctcca aggccccagg gttcagaggt tcagcagtgg
     4861 aactgggggt gtgaaagcca taccctttcc actgtgccaa aatcgtctgc ctacactggc
     4921 agacaggttt gcagtggcct gacacaagca ccttttcttt cacaagaaat ccatctgtgg
     4981 cctcttcacc aaagccatag tgcagatttg aggcactcga cctccatgcc cagagctttt
     5041 cccgtctcaa tattattatt attcattctg acttcggaaa cttggctctg aaacaaatgg
     5101 ttcactaagc taatgatctg ctgtattggt tttgctttca aaaagctcta actgtgccaa
     5161 ggaaatactt gtgtaagatc agattttttt ttttttttta atttactgat cacaaacact
     5221 tggcttacca gcataggcag cctttgtggc tgagcagtat tcccagggtg tgaaacctcc
     5281 ataccctttc caagatagtt actgaagaaa ctgtcttctt gcttagattc acggctgctt
     5341 ttacatttcc tttcactgac agtaaattgt gccactctcc ataccctgta ggacaatgaa
     5401 cacccagaat tcaacccaag acagaagaaa accctcaaat atggcttaag aagctgagtc
     5461 atatggtttg ttcttcctcc tcccaggccc acttgagtag caaaggatcc agcatgtttt
     5521 cctttgcttc ctgcagaggg tagaaactac tcgtagttct ctttccaaca ctccattgca
     5581 tacttgagta taatccccta ggctccaagg cattggagcg cccattctgt aaaagccagc
     5641 ctgcccagga actgcctaac acatccaccc ctgaccaaag tgacacatac tttttccata
     5701 tcaaacaaac atgggaagag aataaggttt ctttaagaaa ggctgtttgt ggttagagct
     5761 acataagaat ctgttaaagg gtagagtata gtcttcattc cccaagataa aaatcactgt
     5821 tacatggaca aatggttatt aggccagatt tgtactcatc tctgtctaga acatttcatg
     5881 tctatgtgtt gcttatgttg tctgtctgca aagtgtctta gctcttttaa tcactgcaaa
     5941 taaagcaata ctgaaaactt gagagagaaa atgcacctta ggggaagggg ccaaatgttt
     6001 ctagagggaa gaggaagacc ttctcctgcc ttcttataag acccagcttg tgtcttaccc
     6061 atgagtttat tagcagggca ggctgtaaca ttcgttctcc cccaactggt cctcagcctt
     6121 ccgtagctac cttaaaagct ggagtttgaa ttgcagggcc tggttgacag gtgccagctt
     6181 cctcgtggca gagcaaaagc tgctgctttt catattctgt tgcctccttt tcttgtggcc
     6241 ccaaggatcc caccaatcct cattccccct aaatgttaaa agaaaattcc ttattgtgga
     6301 tattaagtta cactgtaagc atatttacat gctctttttc ccccctggtt tttcttttca
     6361 tcatgtataa tttgaatcca gtgatagtct cacatcttcc aaaaaagtct gcttatgtga
     6421 tatagaagag aaatattaaa gtagactgaa ggggaacttg tgaaacatta agcattgttc
     6481 tcaaccgttt aatttattga aaggagaagc tgctactgag cagctgctat tcttttgttt
     6541 acatagagtc tggttttgtt tgttttgctc tgtgctggga acataaataa agttttctac
     6601 attattttca gtcataaaaa aaaaaaaaaa a
//
