LOCUS       NM_020333               6042 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus solute carrier family 12, member 5 (Slc12a5),
            transcript variant 3, mRNA.
ACCESSION   NM_020333
VERSION     NM_020333.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   da Cunha Menezes,E., de Abreu,F.F., Davis,J.B., Maurer,S.V.,
            Roshko,V.C., Richardson,A., Dowell,J., Cassella,S.N. and
            Stevens,H.E.
  TITLE     Effects of gestational hypothyroidism on mouse brain development:
            Gabaergic systems and oxidative stress
  JOURNAL   Dev Biol 515, 112-120 (2024)
   PUBMED   39048051
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Ando,H., Shimizu-Okabe,C., Okura,N., Yafuso,T., Kosaka,Y.,
            Kobayashi,S., Okabe,A. and Takayama,C.
  TITLE     Reduced Gene Expression of KCC2 Accelerates Axonal Regeneration and
            Reduces Motor Dysfunctions after Tibial Nerve Severance and
            Suturing
  JOURNAL   Neuroscience 551, 55-68 (2024)
   PUBMED   38788828
  REMARK    GeneRIF: Reduced Gene Expression of KCC2 Accelerates Axonal
            Regeneration and Reduces Motor Dysfunctions after Tibial Nerve
            Severance and Suturing.
REFERENCE   3  (bases 1 to 6042)
  AUTHORS   Zavalin,K., Hassan,A., Zhang,Y., Khera,Z. and Lagrange,A.H.
  TITLE     Region and layer-specific expression of GABAA receptor isoforms and
            KCC2 in developing cortex
  JOURNAL   Front Cell Neurosci 18, 1390742 (2024)
   PUBMED   38894703
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6042)
  AUTHORS   Watanabe,M., Sinha,A.S., Shinmyo,Y. and Fukuda,A.
  TITLE     Early establishment of chloride homeostasis in CRH neurons is
            altered by prenatal stress leading to fetal HPA axis dysregulation
  JOURNAL   Front Mol Neurosci 17, 1373337 (2024)
   PUBMED   38577026
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6042)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 6042)
  AUTHORS   Ehringer,M.A., Thompson,J., Conroy,O., Xu,Y., Yang,F., Canniff,J.,
            Beeson,M., Gordon,L., Bennett,B., Johnson,T.E. and Sikela,J.M.
  TITLE     High-throughput sequence identification of gene coding variants
            within alcohol-related QTLs
  JOURNAL   Mamm Genome 12 (8), 657-663 (2001)
   PUBMED   11471062
REFERENCE   7  (bases 1 to 6042)
  AUTHORS   Hubner,C.A., Stein,V., Hermans-Borgmeyer,I., Meyer,T., Ballanyi,K.
            and Jentsch,T.J.
  TITLE     Disruption of KCC2 reveals an essential role of K-Cl cotransport
            already in early synaptic inhibition
  JOURNAL   Neuron 30 (2), 515-524 (2001)
   PUBMED   11395011
REFERENCE   8  (bases 1 to 6042)
  AUTHORS   Sallinen,R., Tornberg,J., Putkiranta,M., Horelli-Kuitunen,N.,
            Airaksinen,M.S. and Wessman,M.
  TITLE     Chromosomal localization of SLC12A5/Slc12a5, the human and mouse
            genes for the neuron-specific K(+)-Cl(-) cotransporter (KCC2)
            defines a new region of conserved homology
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 67-70 (2001)
   PUBMED   11701957
  REMARK    GeneRIF: Chromosomal localization of SLC12A5/Slc12a5, the human and
            mouse genes for the neuron-specific K(+)-Cl(-) cotransporter (KCC2)
            defines a new region of conserved homology.
            Erratum:[Cytogenet Cell Genet. 2002;98(2-3):232]
REFERENCE   9  (bases 1 to 6042)
  AUTHORS   Vilen,H., Eerikainen,S., Tornberg,J., Airaksinen,M.S. and
            Savilahti,H.
  TITLE     Construction of gene-targeting vectors: a rapid Mu in vitro DNA
            transposition-based strategy generating null, potentially
            hypomorphic, and conditional alleles
  JOURNAL   Transgenic Res 10 (1), 69-80 (2001)
   PUBMED   11252384
REFERENCE   10 (bases 1 to 6042)
  AUTHORS   Haapa,S., Suomalainen,S., Eerikainen,S., Airaksinen,M., Paulin,L.
            and Savilahti,H.
  TITLE     An efficient DNA sequencing strategy based on the bacteriophage mu
            in vitro DNA transposition reaction
  JOURNAL   Genome Res 9 (3), 308-315 (1999)
   PUBMED   10077537
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147262.1 and BC054808.1.
            
            On Oct 26, 2007 this sequence version replaced NM_020333.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (3)
            has a shorter and distinct N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054808.1, AK147262.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2899              AK147262.1         3-2901
            2900-6042           BC054808.1         2566-5708
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.28 cM"
     gene            1..6042
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /note="solute carrier family 12, member 5"
                     /db_xref="GeneID:57138"
                     /db_xref="MGI:MGI:1862037"
     exon            1..467
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..3763
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /note="isoform 3 is encoded by transcript variant 3;
                     mKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter;
                     electroneutral potassium-chloride cotransporter 2"
                     /codon_start=1
                     /product="solute carrier family 12 member 5 isoform 3"
                     /protein_id="NP_065066.2"
                     /db_xref="CCDS:CCDS38332.1"
                     /db_xref="GeneID:57138"
                     /db_xref="MGI:MGI:1862037"
                     /translation="MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGREYDGRN
                     MALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMG
                     VYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVP
                     AGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAED
                     ASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIK
                     SAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEY
                     FTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYV
                     FSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSV
                     VLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPR
                     LLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFL
                     MCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLI
                     AGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQ
                     DQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKG
                     FCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRET
                     TAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKM
                     RIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQ
                     ILKQMHLTKNEREREIQSITDESRGSIRRKNPANPRLRLNVPEETACDNEEKPEEEVQ
                     LIHDQSAPSCPSSSPSPGEEPEGERETDPEVHLTWTKDKSVAEKNKGPSPVSSEGIKD
                     FFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYME
                     FLEVLTEQLDRVMLVRGGGREVITIYS"
     exon            468..562
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            563..694
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            695..841
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            842..896
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1027
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1269
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1481
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1652
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1751
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1809
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1984
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2103
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2202
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2322
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2427
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2596
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2792
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2962
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            2963..3094
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3202
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3203..3325
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3326..3522
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3523..3537
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..3671
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..6023
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5994..5999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /note="hexamer: AATAAA"
     polyA_site      6019
                     /gene="Slc12a5"
                     /gene_synonym="KCC2; mKIAA1176"
                     /note="major polyA site"
ORIGIN      
        1 ggggctgcgt ctgctctagg gaagcgaggg ccgcgtcccc cgcagcatcc cctccatacc
       61 acccgcgcaa gcccccagtt ttcccgtgag ggggccaccg gagctctctg cccgcctctc
      121 tgcccttccc tccctccctc cggtcccccc ctccaaagaa aacccgccag tggctcacgc
      181 ctcctgcata cgggatgagg tgagcagcgc cgctactgag agggggcgcg cgcgggtgtg
      241 agcgtgtgtc cgtgtgcgag tgtgtgtgcg ccgggcgggc gggcactgca gcttcttcct
      301 ccgtggagcg gagagcaagc gagagagctc gagcaagcga gcgagcggag aaggcgggca
      361 gaggggcgcg ggcgaagcgg cgcagccatc ccgagcccgg cgccgcgcag ccaccatgct
      421 caacaacctg acggactgcg aggacggcga tgggggagcc aaccccggtg atggcaaccc
      481 caaagagagc agtcccttca tcaacagcac ggacacggag aagggcagag agtacgatgg
      541 caggaacatg gccctgtttg aggaggagat ggacaccagc cccatggtat cctccctgct
      601 cagtgggctg gccaactaca ccaacctacc ccagggaagt agagagcatg aagaagcaga
      661 aaataatgag ggtggaaaaa agaagccggt gcaggctcct cgaatgggca ccttcatggg
      721 tgtgtacctg ccgtgcctgc agaacatctt tggtgtcatc ctcttcctgc ggctcacgtg
      781 ggtggtgggc atcgcgggca tcatggagtc cttctgtatg gtcttcattt gctgctcctg
      841 tacgatgctc acagccattt ccatgagtgc aatcgcaacc aatggtgttg tgcctgctgg
      901 tggctcgtac tacatgattt ccaggtctct gggcccggag tttgggggcg ccgtgggcct
      961 ctgcttctac ctgggcacca cctttgctgg ggctatgtac atccttggca cgatcgagat
     1021 cctgctggct tatctcttcc cagctatggc catcttcaag gcagaagatg ccagtgggga
     1081 ggcggccgcc atgctgaaca acatgcgggt gtatggcacc tgtgtgctca cctgcatggc
     1141 caccgttgtc tttgtgggtg tcaagtacgt caacaagttt gccttggtct tcctgggttg
     1201 cgtcatcctg tccatcctgg ccatctatgc aggggtcatc aagtctgcct tcgacccacc
     1261 caatttcccg atctgcctcc tggggaaccg cacgctgtct cgccatggct ttgatgtctg
     1321 tgccaagctg gcttgggaag gaaatgagac agtgaccaca cggctctggg gccttttctg
     1381 ctcctcccgc ctcctcaatg ccacctgtga tgagtacttc acccgaaaca atgtcacaga
     1441 gatccagggc attcctggtg ctgccagtgg tctcatcaaa gagaacctgt ggagttctta
     1501 cctgaccaaa ggggtgattg tcgagaggcg tgggatgccc tctgtgggcc tggcagacgg
     1561 tacccccgta gacatggacc acccctatgt cttcagtgat atgacctcct acttcaccct
     1621 gctcgttggt atctacttcc cctcagtcac agggatcatg gctggctcaa accgatctgg
     1681 agacctgcgg gatgcccaga agtctatccc tactggaact atcctggcca ttgctaccac
     1741 ctctgctgtc tacatcagct ctgttgttct gtttggagcc tgcatcgagg gggtcgtctt
     1801 acgggacaag tttggggaag ctgtgaatgg caacttggtg gtgggcaccc tggcctggcc
     1861 ttctccctgg gtcatcgtca taggctcttt cttctctacc tgtggggctg gattacagag
     1921 cctcacaggg gccccacgtc tgctgcaggc catctcccgg gatggcatag tgcccttcct
     1981 gcaggtcttt ggccatggca aagctaatgg agagccaacc tgggcgctgc tgctgactgc
     2041 ctgcatctgt gagatcggca tcctcatagc ctccctggat gaggtcgccc ctatactttc
     2101 catgttcttc ctaatgtgtt acatgtttgt gaacttggct tgtgcggtgc agacgctgct
     2161 gaggacaccc aactggaggc cacgatttcg ctattaccac tggactctct ccttcctggg
     2221 catgagcctc tgcctggccc tcatgttcat ttgctcctgg tactacgcac tggtggccat
     2281 gctcattgcc ggactcattt ataagtacat cgagtaccgg ggggcggaga aggagtgggg
     2341 ggatggaatc cgaggcctgt ctctcagtgc agcacgctat gctctcttgc gcctggagga
     2401 aggacctccg catacgaaga actggaggcc ccagctgctg gtgctggtgc gtgtggacca
     2461 ggatcagaac gtggtgcatc cgcagctgct ctccctgacc tcccagctca aggcagggaa
     2521 gggcctgacc attgtgggct ccgtccttga gggcaccttt ctggacaacc atccacaggc
     2581 tcagcgggca gaggagtcta tcaggcgcct gatggaggct gagaaggtga agggcttctg
     2641 ccaggtagtg atctcctcca acctgcgtga tggtgtgtcc cacctgatcc agtctggggg
     2701 cctcggggga ttgcaacaca ataccgtgct ggtgggctgg cctcgcaact ggaggcagaa
     2761 ggaggatcat cagacatgga ggaacttcat cgaactggtc cgggaaacta cagccggcca
     2821 cctcgccctg ctggtcacca agaatgtttc catgtttccc gggaaccctg agcgcttctc
     2881 ggagggcagc attgacgtgt ggtggattgt gcacgacggg ggcatgctca tgctgctgcc
     2941 cttcctgctg cgacaccaca aggtctggag gaaatgcaaa atgcggatct tcaccgtggc
     3001 ccagatggac gataacagta tccagatgaa gaaggacctg accacgtttc tgtaccactt
     3061 acgcattact gcagaggtgg aggtggtgga gatgcatgag agcgacatct cggcatacac
     3121 ctacgagaag acattagtaa tggagcaacg atctcagatc ctcaaacaga tgcacctcac
     3181 caagaacgag cgggaacggg agatccagag catcacagac gagtctcggg gctccattcg
     3241 gaggaagaat ccagccaacc cccggctccg cctcaatgtt cccgaagaga cagcgtgtga
     3301 caatgaggag aagccagagg aggaggtgca gctgatccat gaccagagtg ctcccagctg
     3361 ccctagcagc tcgccatctc caggggagga gcccgagggg gagagggaga cagacccaga
     3421 ggtgcatctt acctggacca aggataagtc agtggcagag aagaataaag gccccagtcc
     3481 cgtctcctcc gagggcatca aggacttctt cagcatgaag ccggagtggg aaaacttgaa
     3541 ccagtccaat gtacggcgca tgcacacagc tgtgcggctg aacgaggtca tcgtgaataa
     3601 atctcgggat gccaagctag ttttgctcaa catgcccggg cctccccgca accgcaatgg
     3661 ggatgaaaac tacatggaat tcttggaggt cctcactgag caactggacc gggtgatgct
     3721 ggtccgcggt ggcggccgag aggtcatcac catctactcc tgaaggccag gacctgccac
     3781 tccggcccga gcgcgcccgg cccgcggccc ccagagccct cgccgcgcct ccccgccgct
     3841 gtcaccgttt acataagacc cagttgccca tgccctggcc cctttccttc ccgctgcctg
     3901 cagccctgag gccttgcccg tcggggctga cccgcagggc ggcccgtggg ccccttttct
     3961 gagcctggcc tcgccccgcc ggagcagacg ttgcaataaa ggtggcgagg aggcgtggag
     4021 aggagcggaa ccgtggtccc gcgccgggca gccccgagcc cgtccctccc cacgccccac
     4081 cgagctcccc ccgggaccct ggtcgccgag cccgggtgcc gctcggtcgc gctatacata
     4141 gtgtacagga gacatcgcgt gtatttttaa tgtccccata tttctgtaac tagaaacgca
     4201 acggactcct cgccacggcc gcgctctccc cgctgcgggc acccaggaag gcggaagccg
     4261 gggagccagg tttccctgcg ctcccgagct gagagccaag tgctttaagg ccggcgctct
     4321 cctttccctt tcctgtccac agcctgggct accctctcct ccctccagtt cttggcggac
     4381 aaaggtgaga cggtggccgg cttcggtgga tgcgagcttc caggcggcca ggaggggagg
     4441 ggtcgcccct ccagggggag aaactgaggc ccgtgcccac cgaggaagtc ctgcccggtg
     4501 ccttcgtgga ggagcaggcg tctctcctct cttggcttgc cgcctgctcc ccctgtcccg
     4561 tggctcctcg ccaaagactg aattcgtgga gctggagggc acaccctccc cagtttcctt
     4621 cctgggacag gctaggggcc aatgtcagcc ttggggccga ctcaccagga ggcctcgcac
     4681 agcctctgat attccctctc ttggtcccca ttctgcaagt cctgcttggg gacccagctg
     4741 cctggttgtt ctggggctga gctttgctgc ctagcagcag gtccttagcc actgtctcta
     4801 cagataccag gtcctggagt atggatggag tcttacgggc ccagttgttc ctagcgcttc
     4861 tctgaccttt ttctgagtag aactcaaggc ctttggcttt cccaaactca tccttggcgc
     4921 ttccgctcca ccagccccac ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgggt
     4981 gcccttagcc ccttagagag gcgtttggct ggttcccttg ccccagggca cgttactaag
     5041 aggacaggca ctgcatgctc ctttaagcgc cctctgggac tgggtacagt gcctccagcc
     5101 ccagggccct ggcctgtgca cctagttaga catcatgctc actccagggc caggaccact
     5161 agctgacctc accacctctt tttccttgag cccaaggcag agagaactgc agctggtgcc
     5221 atcaaacggc tcaggtgtgg ccagtggcag ggctggaggg ccactgccct gttgcttggc
     5281 tcaagcccct ctcaaggctt gatgaggact gggcattctt ccaggcagta gccatcaagt
     5341 tggaagtgta ggacctaggg cccaccatcc ttcaagtctg ccctcccttg cagtggtttt
     5401 gccttttggg gaaagagagg ggctgggtag ctggggagga ggcagcatcg acaccaatta
     5461 gggaaccaaa gttgcatgac ctgggcccag actctggttg gcaagagcaa agtttccgtt
     5521 gatgaaaaca gcccacaaca aaacccccag ttttctgtgc tccatgtgca atatttgtta
     5581 tgaaccttgt gtcgttcaag tcacctctat aatcactgta gctagatgtt ccatgtccat
     5641 ccaggtgact tctactctga gtgcaatatt tcagtagcct ggtagtgaga agagtgtcgc
     5701 ttctgtttca gcagacctat gtgcagggca atgcaatgca gtccaaaacc cttgtaaata
     5761 ggagaggttg caagccaaat caagagtatt tattgttatt actattatta ttaggcctgc
     5821 ctttaatttt agtgtttcgg tatttcgcat cccgactcgg tattgatctt gtgttctttg
     5881 tgccaatacg caaaggagag ggtcagttct ctcctttact gttgaatgct cccacttaat
     5941 gctttaaggc ttttactgtg ttaatttttt agatacctgt ctgcacacaa tgcaataaaa
     6001 ataattttat tacacccttg ataaaaaaaa aaaaaaaaaa aa
//
