LOCUS       NM_009194               6520 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus solute carrier family 12, member 2 (Slc12a2), mRNA.
ACCESSION   NM_009194 XM_913208
VERSION     NM_009194.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Biggs O'May,J., Vanes,L., de Boer,L.L., Lewis,D.A., Hartweger,H.,
            Kunzelmann,S., Hayward,D., Llorian,M., Kochl,R. and
            Tybulewicz,V.L.J.
  TITLE     WNK1-dependent water influx is required for CD4+ T cell activation
            and T cell-dependent antibody responses
  JOURNAL   Nat Commun 16 (1), 1857 (2025)
   PUBMED   39984435
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   da Cunha Menezes,E., de Abreu,F.F., Davis,J.B., Maurer,S.V.,
            Roshko,V.C., Richardson,A., Dowell,J., Cassella,S.N. and
            Stevens,H.E.
  TITLE     Effects of gestational hypothyroidism on mouse brain development:
            Gabaergic systems and oxidative stress
  JOURNAL   Dev Biol 515, 112-120 (2024)
   PUBMED   39048051
REFERENCE   3  (bases 1 to 6520)
  AUTHORS   Naillat,F., Deshar,G., Hankkila,A., Rak-Raszewska,A., Sharma,A.,
            Prunskaite-Hyyrylainen,R., Railo,A., Shan,J. and Vainio,S.J.
  TITLE     Calcium signaling induces partial EMT and renal fibrosis in a
            Wnt4mCherry knock-in mouse model
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167180 (2024)
   PUBMED   38653356
REFERENCE   4  (bases 1 to 6520)
  AUTHORS   de Boer,L.L., Vanes,L., Melgrati,S., Biggs O'May,J., Hayward,D.,
            Driscoll,P.C., Day,J., Griffiths,A., Magueta,R., Morrell,A.,
            MacRae,J.I., Kochl,R. and Tybulewicz,V.L.J.
  TITLE     T cell migration requires ion and water influx to regulate actin
            polymerization
  JOURNAL   Nat Commun 14 (1), 7844 (2023)
   PUBMED   38057317
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6520)
  AUTHORS   Johnsen,L.O., Friis,K.A. and Damkier,H.H.
  TITLE     In vitro investigation of the effect of proinflammatory cytokines
            on mouse choroid plexus membrane transporters Ncbe and NKCC1
  JOURNAL   Fluids Barriers CNS 20 (1), 71 (2023)
   PUBMED   37828581
  REMARK    GeneRIF: In vitro investigation of the effect of proinflammatory
            cytokines on mouse choroid plexus membrane transporters Ncbe and
            NKCC1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6520)
  AUTHORS   O'Keefe,T.L., Williams,G.T., Davies,S.L. and Neuberger,M.S.
  TITLE     Hyperresponsive B cells in CD22-deficient mice
  JOURNAL   Science 274 (5288), 798-801 (1996)
   PUBMED   8864124
REFERENCE   7  (bases 1 to 6520)
  AUTHORS   Kaplan,M.R., Plotkin,M.D., Brown,D., Hebert,S.C. and Delpire,E.
  TITLE     Expression of the mouse Na-K-2Cl cotransporter, mBSC2, in the
            terminal inner medullary collecting duct, the glomerular and
            extraglomerular mesangium, and the glomerular afferent arteriole
  JOURNAL   J Clin Invest 98 (3), 723-730 (1996)
   PUBMED   8698864
REFERENCE   8  (bases 1 to 6520)
  AUTHORS   Simon,D.B., Karet,F.E., Hamdan,J.M., DiPietro,A., Sanjad,S.A. and
            Lifton,R.P.
  TITLE     Bartter's syndrome, hypokalaemic alkalosis with hypercalciuria, is
            caused by mutations in the Na-K-2Cl cotransporter NKCC2
  JOURNAL   Nat Genet 13 (2), 183-188 (1996)
   PUBMED   8640224
REFERENCE   9  (bases 1 to 6520)
  AUTHORS   Kone,B.C., Schwobel,J., Turner,P., Mohaupt,M.G. and Cangro,C.B.
  TITLE     Role of NF-kappa B in the regulation of inducible nitric oxide
            synthase in an MTAL cell line
  JOURNAL   Am J Physiol 269 (5 Pt 2), F718-F729 (1995)
   PUBMED   7503239
REFERENCE   10 (bases 1 to 6520)
  AUTHORS   Delpire,E., Rauchman,M.I., Beier,D.R., Hebert,S.C. and Gullans,S.R.
  TITLE     Molecular cloning and chromosome localization of a putative
            basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner
            medullary collecting duct (mIMCD-3) cells
  JOURNAL   J Biol Chem 269 (41), 25677-25683 (1994)
   PUBMED   7929272
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ172807.1, U13174.1, CN711123.1 and AC127678.9.
            
            On Sep 25, 2008 this sequence version replaced NM_009194.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U13174.1, SRR10223971.17437.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-238               CJ172807.1         173-410
            239-3308            U13174.1           236-3305
            3309-3714           CN711123.1         186-591
            3715-6520           AC127678.9         146925-149730
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 32.15 cM"
     gene            1..6520
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /note="solute carrier family 12, member 2"
                     /db_xref="GeneID:20496"
                     /db_xref="MGI:MGI:101924"
     exon            1..867
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..3750
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /note="basolateral Na-K-Cl symporter; bumetanide-sensitive
                     sodium-(potassium)-chloride cotransporter 1;
                     sodium/potassium/chloride cotransporters;
                     sodium/potassium/chloride transporters;
                     bumetanide-sensitive sodium-(potassium)-chloride
                     cotransporter 2; Na-K-2Cl cotransporter 1"
                     /codon_start=1
                     /product="solute carrier family 12 member 2"
                     /protein_id="NP_033220.2"
                     /db_xref="CCDS:CCDS37826.1"
                     /db_xref="GeneID:20496"
                     /db_xref="MGI:MGI:101924"
                     /translation="MEPGPAAPSSGAPRPARDGDAPLAAAAGVDLPGTAVPSGQEDAT
                     TAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAA
                     AAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGG
                     DGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRI
                     DHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGV
                     VKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSA
                     IATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH
                     SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIS
                     LESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADP
                     QSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNF
                     DFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALC
                     KDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYAL
                     INFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLY
                     IYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPAL
                     LHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADD
                     LREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVI
                     RLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDE
                     EEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLT
                     LLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINT
                     KPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRL
                     NELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTF
                     YS"
     exon            868..987
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1063
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1159
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1299
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1410
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1519
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1647
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1732
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1884
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1992
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2116
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2218
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2374
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2474
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2586
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2727
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2728..2834
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2914
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            2915..3040
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3088
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3211
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3323
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3410
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3546
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3547..3614
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     exon            3615..6520
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6484..6489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /note="hexamer: AATAAA"
     polyA_site      6520
                     /gene="Slc12a2"
                     /gene_synonym="9330166H04Rik; BSC2; mBSC2; mNKCC1; Nkcc1;
                     sy-ns"
                     /note="major polyA site"
ORIGIN      
        1 gcgctgtgac tctttctctc tctatcactc tcccccggct ccttagcggg agcgccggcc
       61 agggacccga gccgcgccgt acgcagcgtc cacccgcctg ctccgcaggt ccccgagcgt
      121 cgggcggcga tggagcccgg gcccgcggcg ccctcgtctg gcgccccgcg gcccgcccgg
      181 gatggggacg cgccgctggc tgccgccgcc ggggtggacc tgcccggcac ggccgtgccc
      241 tccggccaag aggatgctac tacggctggg aggcaggctg gcggcggggt gcgcggagag
      301 ggaaccccag cggcgggcga tgggctgggc agacctctgg ggcccacccc cagccagagc
      361 cgcttccagg tggacccggt ttccgagaac gccgggcggg ccgcggcggc tgcggcggcg
      421 gcggcggcgg ctgcggctgc ggccggggcg gcgggcaagg agacccccgc ggccgggaaa
      481 gccggcggtg agagcggcgt ggccaagggc agcgaggaag ccaagggccg cttccgcgtg
      541 aacttcgtgg acccggctgc ctcgtcgtcg gcggacgaca gcctgtcgga tgcggcgggg
      601 gtcggtggcg acgggcccaa cgtgagcttc cagaacggcg gggacacggt gctgagcgag
      661 ggcagcagcc tgcactcggg gggtggcagt ggacaccacc agcagtacta ctatgacacc
      721 cacaccaaca cctactacct gcgcaccttc ggccacaaca ccatggacgc ggtgcccagg
      781 atcgaccact accggcacac ggccgcgcag ctgggcgaga agctgctccg gcccagcctg
      841 gcagagctcc acgatgagct ggaaaaggaa ccttttgagg atggctttgc gaatggagaa
      901 gaaagtactc caaccagaga tgctgtggtc gcatacactg ccgagagtaa aggagttgta
      961 aagtttggct ggatcaaggg tgtattagtc cgctgcatgt taaacatttg gggtgtgatg
     1021 ctctttatta gattgtcatg gatcgtgggt caagctggga taggtctctc tgtcgtcgta
     1081 atagcaatgg ccactgttgt gacaactatc acaggattgt ctacttcagc aatagctacc
     1141 aatgggttcg tgagaggagg aggagcgtac tatttaatct ctagaagtct agggccggaa
     1201 tttggtggtg caattggctt gatctttgct tttgccaatg cggtggccgt tgctatgtat
     1261 gttgtcggat tcgcagagac tgtggtggag ctgcttaagg aacattccat acttatgata
     1321 gatgaaatca atgatatccg aattattgga gccattacag tcgtgattct tctgggcatc
     1381 tcagtggctg gaatggagtg ggaagcaaag gctcagattg tgctgctggt gatccttctc
     1441 ctggccatcg ccgacttcgt catagggacg tttatctcac tggagagcaa gaagcccaag
     1501 ggtttctttg gctataaatc tgaaatattt aatgagaact ttgggccgga ttttcgagaa
     1561 gaagagactt tcttctctgt atttgccatc ttttttcctg ctgcaactgg tattctagct
     1621 ggagcaaata tctcaggtga tcttgcagat cctcagtcag ccatacccaa aggaacactc
     1681 ttagccattt tgattactac agtggtttac ataggaattg cagtgtctgt gggttcgtgt
     1741 gttgttcggg atgccactgg caatgttaat gataccatta caacagagct gacaaactgt
     1801 acttctgcag cctgcaaact aaactttgac ttctcttatt gtgagagcaa tacttgttct
     1861 tacgggctaa tgaacaactt tcaggtgatg agcatggtgt caggatttgc acccttgatc
     1921 tctgcaggga tcttttcagc cacgctctcg tcagcactag catctcttgt gagtgctccc
     1981 aaaatatttc aggctctatg taaggacaat atctacccag ctttccagat gtttgctaaa
     2041 ggttatggga aaaataatga accgcttcgt ggttacatct taacatttct gattgcactg
     2101 ggattcatcc tgattgctga attgaacgtt attgctccaa ttatctctaa tttcttcctt
     2161 gcatcgtatg ccttaatcaa cttttcagta ttccatgcat cacttgcaaa atctccagga
     2221 tggcgtcctg cattcaaata ctacaacatg tggatctccc ttattggagc aattctttgt
     2281 tgcatagtga tgtttgtcat taactggtgg gctgcgttgc tcacatatgt gatagtcctt
     2341 gggctgtaca tttatgttac ttacaaaaaa ccagatgtga actggggatc ctccacacaa
     2401 gcgctgactt acctcagtgc actgcagcat tcaatccgtc tttctggggt ggaggaccat
     2461 gtgaaaaact tcagaccaca gtgccttgta atgacaggtt ctccaaactc acggcctgct
     2521 ttacttcatc ttgttcatga cttcacgaaa aatgttggtt taatgatctg tggccatgtg
     2581 catatgggcc ccagaagaca agctatgaaa gagatgtcca ttgatcaagc cagatatcag
     2641 cgatggctca ttaaaaacaa aatgaaggct ttctatgctc cggttcacgc agatgacttg
     2701 cgagaaggtg cacaatactt gatgcaggct gctggacttg gtcgtatgaa gccaaacaca
     2761 cttgtcctgg gatttaagaa agattggtta caagccgata tgagggatgt ggatatgtat
     2821 ataaacttat ttcatgatgc ttttgacata caatttggag ttgtggttat ccgcctgaag
     2881 gaaggactgg atatatcaca cctccaaggt caggaagaat tattatcttc acaagaaaaa
     2941 tcacctggta ccaaggatgt ggtagtaaat gtggattaca gtaaaaagtc agatcaagat
     3001 acttgcaaat catctggtga aaaatccatt acacagaaag atgaggaaga ggatggcaag
     3061 actccaactc agccactgtt gaaaaaagaa tccaaaggcc ctattgtgcc tttaaatgta
     3121 gctgaccaaa agcttcttga agctagcaca cagtttcaga aaaaacaagg gaagaatact
     3181 atagatgtct ggtggctttt tgatgatgga ggtttgacat tgttgatacc ctatcttctg
     3241 actaccaaga aaaagtggaa agattgtaag atccgagtat tcattggtgg aaaaataaac
     3301 agaatagacc atgaccggag agcgatggct actttattga gtaaattccg gatagacttc
     3361 tctgatatca tggtccttgg agatatcaac accaagccaa aaaaagaaaa tattattgct
     3421 tttgatgaca tgatcgagcc gtacagactt cacgaagatg ataaagaaca ggacattgca
     3481 gataagatga aagaagatga gccttggcga atcacagata atgagcttga gctttataag
     3541 actaagacat atcgacagat caggttaaat gaattattaa aggagcattc aagcacagct
     3601 aacattattg tcatgagtct tccagtcgca cggaaaggcg ctgtgtccag tgctctgtat
     3661 atggcttggt tagaagctct ctcgaaggac ctgccaccag tcctcctggt tcgtgggaat
     3721 catcagagcg tccttacctt ctactcataa ctgttctgca ccatggactt cctctaagga
     3781 tggcacttca gtgcccagtc aaagtgactg tatcgtcagg aacaccacag catcgcagtg
     3841 gcagacgggg actgcttcac tacttcatta ttggaaagca catggaaagt tgccccattg
     3901 atagatagat ggagacttca gttgtagtct actccagatc tcaatcttaa tgactgctaa
     3961 ttctttcttg ttggactgaa gttcatgaga gtaaaatttt cctttgctac ttgaaagcaa
     4021 taagagtgag ttattttttg attgctgaaa ggagtgcaaa gcctttagcc ttgaggtgcc
     4081 ttctgaaata accaaatttc atccagagac ctgtcgtctt ttgtaaattt ctagaatgtt
     4141 aaactctgcc ttcagatgtt attcttgttt ttaatctctg ttatcctaag tggacactgc
     4201 ttttctcctc ccaccgacca gttagtgttg agaactgtgt gttttgtatt acttttgtaa
     4261 aagtatctgt tagatactga agaggattag gacaagctaa taaaatatgt ggggaaagta
     4321 tccaaaaaat ggagcccaag gttaggttta tatgaatata tatatataca tatatagagg
     4381 gagagctagg aaaaagaggc ccagaccaac tttcggttag aattatgaat tatttctttt
     4441 aaatcataga gatgactgat gatctaccca gtaattcttg tataatgctt ttaaaaaaga
     4501 atcatgtttt ttttttaaac aaaaccgtga gatcatttgt attattttta aaagatctct
     4561 tgatttagaa ttttctctca aatgtgaggg acttttatga catgctaaca tatttttttt
     4621 agaggaaatt tagacaaagc agtgccattt aatagaacat ttcaataaac actatgggga
     4681 tttcactgtc ctatgccatc ctttatttaa aaaaaaaaga ttaaaatttt accaatggtt
     4741 aaaaggagca ctgtttcctt agtgaaatgg ttttaggctt catttattca agtatcaagt
     4801 acagttagtt ttaatacact caggattgaa cagattgttc tgaacattga aacttaatcc
     4861 attcttaata ctttaaaact tgtgttaaag aaaactgcca gtttgtgcgt ttgaccttgt
     4921 ctgctaatgc actcgacagc gtgcatgtgc acatgtgctg cctgaaagct ttatatgcag
     4981 acagacagcg cgcatgtgca cacgtgctat cctgaaagct ttatatacag ttgtcagtgt
     5041 gatgtttctc agcttcaagg aaggattttc taaaaacatt tcaagggatt catatctaca
     5101 tgtctgtatg tgtgtatgtt atacatacac aggtgcatat gtgtatatgt aatgaaattt
     5161 atgttgccag tgtttcagat tttaaagtga tcacgatttg gtagattaca gatatttttt
     5221 tgttgttgtt ttagtttttt ctttttcttt ttttaagtaa acagtcaagt caggtcaagc
     5281 aagacgttaa agatttcacg gaggacagag acaatttgaa gaccatagtg tgacttcata
     5341 cagttatcca gttaggtaaa gggctaacaa gtggatgatc tctaagtttc ggtggagaac
     5401 ggtcccagat ttttgtcatc ttaagggaaa tgtcagaaaa gcgttctcag ttgtggggta
     5461 cttgtgctgc gcggatgcag actgggtcag taccagccgg atatctactc atttccattc
     5521 agaatattaa gaaaacaggt taaatgattt ttaaaaaaag ttttatttac attataatac
     5581 aatattttcc atttgttgta aattttgcag agtttagggc agtataaaag agtcccacca
     5641 ggaaaccata ccatgcgtga atttgctgaa tggaagctag ggcgtgggcc gagcgctgct
     5701 atcttgaaat gtgcacaggt agacttgcct gccttttact tttaaagctt cccccccccc
     5761 ccattcagga aacttcactg tgacctgtat gacagaaact aatatcatac atgttacacg
     5821 tgtgtttaaa ggacacacag tgaatctcga tgcacctaac agggagggag ggtgatactg
     5881 tttgtgatgt gatgttagca gcaggtttcc acctttaaaa actaccatta cggtggctaa
     5941 cactggctgc ctggtcaaac agtctcaagg ttgcagtcgt actctataaa gtgaaaattc
     6001 tcaaccatta gttcttagag atgctagcta gcattaaagc tatttttatt ttatgccgac
     6061 ttttgattat tttgatgcca aattattttt tagtcctaat cgttgataat aacttaaaaa
     6121 taattatgca gtggcctttg agctcattat tatgataggt aaaacatccg gtgggttcaa
     6181 aggggacatt ggtgttgatt attaacaggt aactaaatca gatatttatt tgttgcgtct
     6241 ttctctttgt gcttttaagt tttcctctct actttttttc atattgcttt ctgacgtgtg
     6301 tatgtgtgtg tgtgtgtatt ttttttaaga ctatggaaat aatttaaaga gttgagctct
     6361 ggtgattatc tgctaagttt gaaatgtaat attttgataa tactgtactg ttcctgtgac
     6421 agacgccttt gtaatgtttt aaccctgagc attgcggttg ctgctttgta cagaagttac
     6481 tgcaataaag gaagtggagt catcacaagt ttaatactta
//
