LOCUS       NM_001079690            4645 bp    mRNA    linear   ROD 07-JAN-2025
DEFINITION  Mus musculus solute carrier family 12, member 1 (Slc12a1),
            transcript variant 3, mRNA.
ACCESSION   NM_001079690
VERSION     NM_001079690.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ide,H., Miike,K., Ohmori,T., Maruyama,K., Izumi,Y., Tanigawa,S. and
            Nishinakamura,R.
  TITLE     Mouse embryonic kidney transplantation identifies maturation
            defects in the medulla
  JOURNAL   Sci Rep 14 (1), 30293 (2024)
   PUBMED   39639083
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Ma,Y.S., Deng,S.Q., Zhang,P., Thomsen,J.S., Andreasen,A.,
            Chang,S.J., Zhang,J., Gu,L. and Zhai,X.Y.
  TITLE     Identification of countercurrent tubule-vessel arrangements in the
            early development of mouse kidney based on immunohistochemistry and
            computer-assisted 3D visualization
  JOURNAL   PLoS One 19 (8), e0307223 (2024)
   PUBMED   39137214
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4645)
  AUTHORS   Huang,B., Zeng,Z., Kim,S., Fausto,C.C., Koppitch,K., Li,H., Li,Z.,
            Chen,X., Guo,J., Zhang,C.C., Ma,T., Medina,P., Schreiber,M.E.,
            Xia,M.W., Vonk,A.C., Xiang,T., Patel,T., Li,Y., Parvez,R.K.,
            Der,B., Chen,J.H., Liu,Z., Thornton,M.E., Grubbs,B.H., Diao,Y.,
            Dou,Y., Gnedeva,K., Ying,Q., Pastor-Soler,N.M., Fei,T.,
            Hallows,K.R., Lindstrom,N.O., McMahon,A.P. and Li,Z.
  TITLE     Long-term expandable mouse and human-induced nephron progenitor
            cells enable kidney organoid maturation and modeling of plasticity
            and disease
  JOURNAL   Cell Stem Cell 31 (6), 921-939 (2024)
   PUBMED   38692273
REFERENCE   4  (bases 1 to 4645)
  AUTHORS   Naillat,F., Deshar,G., Hankkila,A., Rak-Raszewska,A., Sharma,A.,
            Prunskaite-Hyyrylainen,R., Railo,A., Shan,J. and Vainio,S.J.
  TITLE     Calcium signaling induces partial EMT and renal fibrosis in a
            Wnt4mCherry knock-in mouse model
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (5), 167180 (2024)
   PUBMED   38653356
REFERENCE   5  (bases 1 to 4645)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 4645)
  AUTHORS   Zuberi,A.R., Nguyen,H.Q., Auman,H.J., Taylor,B.A. and
            Roopenian,D.C.
  TITLE     A genetic linkage map of mouse chromosome 2 extending from
            thrombospondin to paired box gene 1, including the H3 minor
            histocompatibility complex
  JOURNAL   Genomics 33 (1), 75-84 (1996)
   PUBMED   8617512
REFERENCE   7  (bases 1 to 4645)
  AUTHORS   Pathak,B.G., Shaughnessy,J.D. Jr., Meneton,P., Greeb,J.,
            Shull,G.E., Jenkins,N.A. and Copeland,N.G.
  TITLE     Mouse chromosomal location of three epithelial sodium channel
            subunit genes and an apical sodium chloride cotransporter gene
  JOURNAL   Genomics 33 (1), 124-127 (1996)
   PUBMED   8617496
REFERENCE   8  (bases 1 to 4645)
  AUTHORS   Igarashi,P., Vanden Heuvel,G.B., Payne,J.A. and Forbush,B. 3rd.
  TITLE     Cloning, embryonic expression, and alternative splicing of a murine
            kidney-specific Na-K-Cl cotransporter
  JOURNAL   Am J Physiol 269 (3 Pt 2), F405-F418 (1995)
   PUBMED   7573490
REFERENCE   9  (bases 1 to 4645)
  AUTHORS   Quaggin,S.E., Payne,J.A., Forbush,B. 3rd and Igarashi,P.
  TITLE     Localization of the renal Na-K-Cl cotransporter gene (Slc12a1) on
            mouse chromosome 2
  JOURNAL   Mamm Genome 6 (8), 557-558 (1995)
   PUBMED   8589530
REFERENCE   10 (bases 1 to 4645)
  AUTHORS   Gamba,G., Miyanoshita,A., Lombardi,M., Lytton,J., Lee,W.S.,
            Hediger,M.A. and Hebert,S.C.
  TITLE     Molecular cloning, primary structure, and characterization of two
            members of the mammalian electroneutral sodium-(potassium)-chloride
            cotransporter family expressed in kidney
  JOURNAL   J Biol Chem 269 (26), 17713-17722 (1994)
   PUBMED   8021284
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK052750.1, AL929451.8, BY716143.1 and AL844547.9.
            
            On Feb 2, 2019 this sequence version replaced NM_001079690.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U94518.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-833               AK052750.1         22-854
            834-929             AL929451.8         73317-73412
            930-1069            AL929451.8         76057-76196
            1070-1180           AL929451.8         77301-77411
            1181-1714           BY716143.1         30-563
            1715-1765           AL929451.8         92912-92962
            1766-1889           AL929451.8         93269-93392
            1890-1991           AL929451.8         94811-94912
            1992-2147           AL929451.8         96834-96989
            2148-2247           AL929451.8         100769-100868
            2248-2359           AL929451.8         101641-101752
            2360-2500           AL929451.8         107197-107337
            2501-2607           AL929451.8         110063-110169
            2608-2690           AL929451.8         112659-112741
            2691-2834           AL929451.8         120653-120796
            2835-2966           AL844547.9         2418-2549
            2967-3078           AL844547.9         2775-2886
            3079-3165           AL844547.9         4371-4457
            3166-3301           AL844547.9         10562-10697
            3302-3369           AL844547.9         12277-12344
            3370-4645           AL844547.9         13361-14636
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.23 cM"
     gene            1..4645
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /note="solute carrier family 12, member 1"
                     /db_xref="GeneID:20495"
                     /db_xref="MGI:MGI:103150"
     exon            1..34
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            35..625
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     CDS             233..3505
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /note="isoform F is encoded by transcript variant 3;
                     bumetanide-sensitive cotransporter type 1;
                     bumetanide-sensitive sodium-(potassium)-chloride
                     cotransporter 2; BSC1; kidney-specific Na-K-Cl symporter;
                     Na-K-2Cl cotransporter 2; bumetanide-sensitive
                     sodium-(potassium)-chloride cotransporter 1"
                     /codon_start=1
                     /product="solute carrier family 12 member 1 isoform F"
                     /protein_id="NP_001073158.2"
                     /db_xref="CCDS:CCDS50694.1"
                     /db_xref="GeneID:20495"
                     /db_xref="MGI:MGI:103150"
                     /translation="MSVSIPSNSVPSSASRFQVHVINEGHGSAAAVGDSADPPHYEET
                     SFGDEAQNRLRISFRPGNQECYDNFLQTGETAKTDTTFHAYDSHTNTYYLQTMDAVPK
                     IEYYRNTGSVSGPKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEE
                     DMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTG
                     ISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVD
                     LLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFI
                     GTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANIS
                     GDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSA
                     ACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPK
                     VFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFF
                     LASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYV
                     IELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGP
                     MTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYA
                     AVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFE
                     IGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAG
                     KLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLT
                     LLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINI
                     KPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRL
                     NELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTF
                     YS"
     exon            626..757
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            758..833
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            834..929
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1069
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1180
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1292
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1420
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1505
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1657
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1765
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1889
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1991
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2147
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2247
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2359
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2500
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2607
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2690
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2691..2834
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2966
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            2967..3078
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            3079..3165
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..3301
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            3302..3369
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..4645
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4612..4617
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /note="hexamer: AATAAA"
     polyA_site      4645
                     /gene="Slc12a1"
                     /gene_synonym="D630042G03Rik; mBSC1; mNKCC2; Nkcc2;
                     urehr3"
                     /note="major polyA site"
ORIGIN      
        1 actgttgctc agaagaaggc tgctggatgg ggcgcagcct tgaggaccat tctgaagttt
       61 gcatgggaga ccacatatca acaatacatc agggatctag ctatcgggtc atctaaaacc
      121 gaagccaagc gctccgtatt ataaccacac tattgtctat tttaaagcag aaatacgtag
      181 attaaaacaa caaccactat aaaataggta gttccacagt agattttgga agatgtcggt
      241 cagcatccct tccaactcag tgcccagtag tgccagtcgc tttcaggtcc atgttataaa
      301 cgagggccat ggcagtgcgg cagccgtggg cgacagtgca gaccccccac attatgaaga
      361 gacctctttc ggggacgaag cccaaaacag actgagaatc agctttaggc ctgggaatca
      421 ggagtgctat gacaattttc tccaaactgg agagactgcc aaaacagaca ccacgtttca
      481 cgcctacgac tctcacacaa acacatacta cctacaaacc atggacgccg tccccaagat
      541 tgaatactac cgcaacacag gcagcgtgag cgggcccaag gtcaaccggc ccagcctgct
      601 ggagatccac gagcagctgg ctaagaatgt gactgtggcc cctggttcag ctgacagggt
      661 tgctaatgga gatgggatgc ctggagatga gcaagcagaa aacaaggaag aagacatgac
      721 tggagttgtg aagtttggat gggtgaaagg tgtgctggtg cgatgcatgc tgaacatctg
      781 gggagtcatg ctcttcattc gcctctcctg gattgtagga gaagcgggaa ttggtctggg
      841 cgtcatcatc attggcctga gcgtagttgt gacaacactc acaggtattt ctatgtcggc
      901 tatttgcaca aacggagtgg tgcgaggagg tggagcctac tacctgattt cccggagctt
      961 agggcctgag ttcggtgggt caataggctt gatctttgct tttgccaatg cagtggctgt
     1021 cgctatgtat gtggtgggat tcgcggagac tgtcgtggat cttcttaagg agagcgattc
     1081 aatgatggtg gatccaacca atgacatccg catcatcggt tccatcaccg tggtgattct
     1141 tctaggaatc tcagtagctg ggatggagtg ggaagcaaag gctcaagtca ttctcctggt
     1201 cattcttctc attgccattg caaacttctt cattggaact gtcatcccat ccaacaatga
     1261 gaagaaatcc agaggcttct ttaactacca agcatctata tttgcggaaa actttgggcc
     1321 aagcttcaca aagggggaag gcttcttctc ggtctttgcc atctttttcc cagctgctac
     1381 tgggattctt gctggtgcca acatctctgg agacctggag gatccccagg acgccatccc
     1441 cagagggacc atgctggcca tcttcatcac caccgtggcc tacataggtg ttgctatttg
     1501 cgtagccgcc tgtgtggtcc gagatgccac tggcagcatg aatgacacca tcgtttctgg
     1561 gatgaattgc aatggctctg cggcctgtgg gttgggctac gacttctcaa gatgtcaaca
     1621 cgagccatgt cagtatgggc tgatgaacaa tttccaggtc atgagcatgg tgtccgggtt
     1681 cgggcccctc atcactgcag ggatcttttc ggcaacactg tcttccgccc tggcctccct
     1741 cgtcagtgcg cccaaagtat tccaggctct gtgcaaggac aacatcttca aaggactgca
     1801 gttcttcgca aagggctatg ggaaaaacaa cgagcccctg agagggtact tcctcacttt
     1861 tgtgatagcc atggcattca ttctcatcgc cgagctgaat gtcattgccc ccatcatctc
     1921 caacttcttc ctggcctcat atgcgcttat taatttctcc tgtttccatg cctcgtatgc
     1981 caaatctcca ggatggaggc ctgcatatgg gatttacaac atgtgggtat ctctttttgg
     2041 agcgattttg tgctgtgcag tcatgtttgt catcaactgg tgggcagctg tcatcactta
     2101 tgtcattgag ttattcctct atatctatgt gacttataag aagccagatg tgaactgggg
     2161 ctcctccaca caggctctgt cctatgtgag tgctttagac aacgctctgg aattaaccac
     2221 agtggaagac catgtgaaaa acttcagacc ccagtgcatt gtcttaacag gcggacccat
     2281 gactagaccc gctctcttgg atataaccca cgcctttacg aagaatagtg gcctctgcat
     2341 ctgctgcgaa gtcttcgtgg gacctcgcaa gctgtgtgtt aaagagatga acagcggcat
     2401 ggctaaaaaa caggcctggc ttataaagaa caaaatcaaa gctttttatg ctgcagtggc
     2461 tgcagactgc ttcagggacg gtgtccggag cctccttcag gcctcaggct tggggagaat
     2521 gaagccaaac actctcgtga ttggatacaa aaagaactgg aggaaagctc ccttgtcaga
     2581 gctggagaac tacgtgggaa tcatacatga cgcctttgac tttgagattg gcgtggtcat
     2641 agtcagaatc agccaaggct ttgacatctc tccggttctc caggtacaag acgaactgga
     2701 gaaactggaa caggagagat tggctctgga ggccgcaatc aaagacaacg agtgtgagga
     2761 gggcaagggg ggcatccgag gcttgtttaa gaaggccggc aagctgaaca ttaccaagcc
     2821 agcccccaag aaagacggca acatcagcag catccagtca atgcacgtcg gggagttcaa
     2881 tcagaaactg gtggaagcca gcgctcagtt taaaaagaag cagggaaaag gcacgattga
     2941 tgtttggtgg ttatttgatg atggagggtt aacactgctt atcccatata tcttgactct
     3001 cagaaaaaaa tggaaagatt gtaagttaag aatctatgtt ggaggaaaga tcaaccgcat
     3061 cgaagaggaa aaaatttcaa tggcttcact tctcagcaaa tttagaataa aatttgcaga
     3121 catccatata attggagaca tcaacattaa gccaaacaaa gagagctgga aagtctttga
     3181 agagatgatt gaaccttatc ggcttcatga aagccacaaa gatttgacca ctgctgagaa
     3241 attaaaaaga gaaagtccat ggaaaattac agatgcagaa ctggaagcag tcaaggaaaa
     3301 gagttaccgt caagttcgac tgaacgagct cttacaggaa cactccagag ccgccaacct
     3361 catcgtgttg agccttccag tggcaagaaa aggatctatt tcggatttgt tgtacatggc
     3421 gtggttagaa atcctgacca agaaccttcc tcctgtgtta ctggttagag gaaatcacaa
     3481 aaatgtctta acattttact cttaaagcct gaaggatgag aaatgttgtg ccttggtgtc
     3541 tgaataattc tgaaacacat ctggttcttt gtgttttaaa acctcttcta tgaatataca
     3601 aacctctgga gactaaccta accgattcta cataagtttt gtcttttaaa aaaaagttat
     3661 tattaatggg aggttttttt cccactagct taaaaaacat tgaaagccat gagactccta
     3721 gaaaaacact tttccgttgt tgctggtaaa taagaaatta ataaaagctg gctgacatgc
     3781 ttacaggata tgctaatcta gctgtgagct ccaggcaaac tttatctcgt ttctgtagag
     3841 tctgccgcat ggccctggtg caggccacag gcccacagcg ggctctcctt aagttcttat
     3901 tgacaggctg gtatggtgaa ggcaggtggc tatcatagga aaaggcctgc tgggtcagtg
     3961 gcctgattct gcttggcctc ctgagaagtt tgtgaagatt taaaaatcaa tatctggaaa
     4021 ctctaagaag gactgaagtc tacaaagtgt tcaaagcagt tacctaaata aggtttattt
     4081 aatgcagcgg ataataagct taccctctga tcacatgtag cttacacttg ctgctagctg
     4141 gaggtatatt aggtaggtga gcaagagacc catgttccac ttacatgaaa tagagtctta
     4201 acatacaaat atctattgtc ttcctggcta aatattttgg tatttctacc ttagtactac
     4261 tttatttttt tctctctcta agtggtatgc atagatgaaa taaaaatcga tgatctcata
     4321 gaaaattaat ttcacaggtt tccaatccac tattaaaaat gtatccagat cctacattta
     4381 tttactttct aaatatatag gtatttaacc cgtgctttac agaataacaa agatataaat
     4441 ggagacacat gtaaaattta aaaatagagg tgctgatttt tcttcttgtt agtgtgtgta
     4501 taatatttaa tcacagaaaa aatgcttata tgataaatgg cttgcttttg gtttgtgtca
     4561 tttcttggca tattttgact gggggatgtt cgtgggggca aattgacctg aaataaaatc
     4621 actacatttt gtttgctttg gcaaa
//
