LOCUS       NM_008732               3041 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus solute carrier family 11 (proton-coupled divalent
            metal ion transporters), member 2 (Slc11a2), transcript variant 2,
            mRNA.
ACCESSION   NM_008732
VERSION     NM_008732.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Robertson,K.V., Rodriguez,A.S., Cartailler,J.P., Shrestha,S.,
            Schleh,M.W., Schroeder,K.R., Valenti,A.M., Kramer,A.T.,
            Harrison,F.E. and Hasty,A.H.
  TITLE     Knockdown of microglial iron import gene, Slc11a2, worsens
            cognitive function and alters microglial transcriptional landscape
            in a sex-specific manner in the APP/PS1 model of Alzheimer's
            disease
  JOURNAL   J Neuroinflammation 21 (1), 238 (2024)
   PUBMED   39334471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Fenaroli,F., Valerio,A. and Ingrassia,R.
  TITLE     Ischemic Neuroprotection by Insulin with Down-Regulation of
            Divalent Metal Transporter 1 (DMT1) Expression and Ferrous
            Iron-Dependent Cell Death
  JOURNAL   Biomolecules 14 (7), 856 (2024)
   PUBMED   39062570
  REMARK    GeneRIF: Ischemic Neuroprotection by Insulin with Down-Regulation
            of Divalent Metal Transporter 1 (DMT1) Expression and Ferrous
            Iron-Dependent Cell Death.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3041)
  AUTHORS   Lv,B., Fu,P., Wang,M., Cui,L., Bao,L., Wang,X., Yu,L., Zhou,C.,
            Zhu,M., Wang,F., Pang,Y., Qi,S., Zhang,Z. and Cui,G.
  TITLE     DMT1 ubiquitination by Nedd4 protects against ferroptosis after
            intracerebral hemorrhage
  JOURNAL   CNS Neurosci Ther 30 (4), e14685 (2024)
   PUBMED   38634270
  REMARK    GeneRIF: DMT1 ubiquitination by Nedd4 protects against ferroptosis
            after intracerebral hemorrhage.
REFERENCE   4  (bases 1 to 3041)
  AUTHORS   Liu,H., Chi,R., Xu,J., Guo,J., Guo,Z., Zhang,X., Hou,L., Zheng,Z.,
            Lu,F., Xu,T., Sun,K. and Guo,F.
  TITLE     DMT1-mediated iron overload accelerates cartilage degeneration in
            Hemophilic Arthropathy through the mtDNA-cGAS-STING axis
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (4), 167058 (2024)
   PUBMED   38331112
  REMARK    GeneRIF: DMT1-mediated iron overload accelerates cartilage
            degeneration in Hemophilic Arthropathy through the mtDNA-cGAS-STING
            axis.
REFERENCE   5  (bases 1 to 3041)
  AUTHORS   Volk Robertson,K., Schleh,M.W., Harrison,F.E. and Hasty,A.H.
  TITLE     Microglial-specific knockdown of iron import gene, Slc11a2, blunts
            LPS-induced neuroinflammatory responses in a sex-specific manner
  JOURNAL   Brain Behav Immun 116, 370-384 (2024)
   PUBMED   38141840
  REMARK    GeneRIF: Microglial-specific knockdown of iron import gene,
            Slc11a2, blunts LPS-induced neuroinflammatory responses in a
            sex-specific manner.
REFERENCE   6  (bases 1 to 3041)
  AUTHORS   Beier,D.R., Morton,C.C., Leder,A., Wallace,R. and Leder,P.
  TITLE     Perinatal lethality (ple): a mutation caused by integration of a
            transgene into distal mouse chromosome 15
  JOURNAL   Genomics 4 (4), 498-504 (1989)
   PUBMED   2744761
REFERENCE   7  (bases 1 to 3041)
  AUTHORS   Handa,S., Ferguson,J.M., Wallace,M.E. and Bulfield,G.
  TITLE     Characterization and mapping of a viable anaemic mutant in the
            mouse: a new allele, mkvan, at the microcytic anaemia locus
  JOURNAL   Genet Res 51 (1), 41-45 (1988)
   PUBMED   3366378
REFERENCE   8  (bases 1 to 3041)
  AUTHORS   Sassa,S. and Bernstein,S.E.
  TITLE     Studies of erythrocyte protoporphyrin in anemic mutant mice: use of
            a modified hematofluorometer for the detection of heterozygotes for
            hemolytic disease
  JOURNAL   Exp Hematol 6 (5), 479-487 (1978)
   PUBMED   658175
REFERENCE   9  (bases 1 to 3041)
  AUTHORS   Bannerman,R.M.
  TITLE     Genetic defects of iron transport
  JOURNAL   Fed Proc 35 (11), 2281-2285 (1976)
   PUBMED   782924
  REMARK    Review article
REFERENCE   10 (bases 1 to 3041)
  AUTHORS   Edwards,J.A. and Hoke,J.E.
  TITLE     Red cell iron uptake in hereditary microcytic anemia
  JOURNAL   Blood 46 (3), 381-388 (1975)
   PUBMED   807277
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ057283.1, AK049856.1 and L33415.1.
            
            On Mar 31, 2009 this sequence version replaced NM_008732.1.
            
            Transcript Variant: This variant (2) differs in the 3' coding
            region and 3' UTR, compared to variant 1. These differences result
            in a protein (isoform 2) with a longer and distinct C-terminus,
            compared to isoform 1. This variant does not have a stem-loop iron
            response element (IRE) in the 3' UTR.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019137.1, L33415.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                CJ057283.1         2-54
            54-1761             AK049856.1         2-1709
            1762-2432           L33415.1           1690-2360
            2433-2822           L33415.1           2363-2752
            2823-3041           L33415.1           2754-2972
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.29 cM"
     gene            1..3041
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="solute carrier family 11 (proton-coupled divalent
                     metal ion transporters), member 2"
                     /db_xref="GeneID:18174"
                     /db_xref="MGI:MGI:1345279"
     exon            1..94
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            95..166
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1839
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="isoform 2 is encoded by transcript variant 2;
                     DMT-1; NRAMP 2; divalent metal transporter 1; divalent
                     cation transporter 1; microcytic anemia, viable anaemia;
                     natural resistance-associated macrophage protein 2; solute
                     carrier family 11 member 2"
                     /codon_start=1
                     /product="natural resistance-associated macrophage protein
                     2 isoform 2"
                     /protein_id="NP_032758.2"
                     /db_xref="CCDS:CCDS37211.1"
                     /db_xref="GeneID:18174"
                     /db_xref="MGI:MGI:1345279"
                     /translation="MVLDPKEKMPDDGASGDHGDSASLGAINPAYSNSSLPHSTGDSE
                     EPFTTYFDEKIPIPEEEYSCFSFRKLWAFTGPGFLMSIAYLDPGNIESDLQSGAVAGF
                     KLLWVLLLATIVGLLLQRLAARLGVVTGLHLAEVCHRQYPKVPRIILWLMVELAIIGS
                     DMQEVIGSAIAINLLSAGRVPLWGGVLITIADTFVFLFLDKYGLRKLEAFFGFLITIM
                     ALTFGYEYITVKPSQSQVLRGMFVPSCPGCRTPQVEQAVGIVGAVIMPHNMYLHSALV
                     KSRQVNRANKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFEKTNKQVVEVC
                     KNNSSPHADLFPSDNSTLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTY
                     SGQFVMEGFLNLKWSRFARVILTRSIAIIPTLLVAVFQDVEHLTGMNDFLNVLQSLQL
                     PFALIPILTFTSLRPVMSEFSNGIGWRIAGGILVLIVCSINMYFVVVYVQELGHVALY
                     VVAAVVSVAYLTFVFYLGWQCLIALGLSFLDCGRSYRLGLTAQPELYLLNTVDADSVV
                     SR"
     exon            167..315
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            316..441
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            442..561
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            562..668
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            669..739
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            740..807
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            808..963
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1122
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1209
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1329
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1479
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1553
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1707
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1761
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..3025
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2140..2145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="hexamer: TATAAA"
     polyA_site      2165
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="major polyA site"
     regulatory      2999..3004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="hexamer: AATAAA"
     polyA_site      3025
                     /gene="Slc11a2"
                     /gene_synonym="DCT1; DMT1; mk; Nramp2; van"
                     /note="major polyA site"
ORIGIN      
        1 ggccaatcac gggagggcag gaggttgact gggtcgccgc tggctcctgg gatatggagt
       61 cgccggcgcg gcgtgtgtgg aggtggcgga gccgaatcct attctaagag cacagaccct
      121 caggtatcta ccatggtgtt ggatcctaaa gaaaagatgc cagacgatgg cgcttctggg
      181 gaccatggag actctgccag ccttggcgcc atcaaccctg cctacagcaa ctcatccctc
      241 ccacattcca ctggagactc tgaggagccc ttcaccacct actttgatga gaaaatcccc
      301 attcctgagg aggagtactc ttgttttagc tttcgtaaac tctgggcgtt cacggggcct
      361 ggctttctta tgagcattgc ctacctagac ccaggaaaca tcgaatctga tttgcagtct
      421 ggagcagtgg ctggatttaa gctgctctgg gtgctcctct tggccaccat tgtggggctg
      481 ctgctccagc gccttgcagc gagacttgga gtggtcaccg gcttgcatct tgctgaagta
      541 tgtcaccgtc agtatcccaa ggtcccacgg atcatcctgt ggctgatggt ggagttggca
      601 atcattggtt ctgacatgca ggaagtcatt ggctcagcca tcgccatcaa tctcctgtct
      661 gcaggaaggg tccccctgtg gggcggagtc ctcatcacca tcgcagacac ttttgtgttt
      721 ctttttttgg acaaatatgg cttgcggaag ctggaagcgt tttttggctt tctcatcact
      781 atcatggccc tcacgtttgg atatgagtac attacagtga agcccagcca gagccaagta
      841 ctcaggggca tgttcgtgcc gtcctgtcca gggtgccgca cccctcaggt ggagcaggcg
      901 gtgggcatcg tgggagctgt gatcatgccg cacaacatgt acctgcattc tgccttagtc
      961 aagtctagac aggtgaatcg ggccaataag caggaagtgc gggaagccaa taagtacttc
     1021 ttcatcgagt cctgcatcgc gctctttgtt tccttcatca tcaatgtctt tgtcgtgtcc
     1081 gtctttgctg aagcattttt tgagaaaacc aacaagcagg tggttgaagt ctgcaaaaat
     1141 aacagcagcc cccatgctga cctctttccc agtgacaact ctactctggc tgtggacatc
     1201 tacaaagggg gtgttgtgct tggatgttac ttcgggcctg cagctctcta catctgggca
     1261 gtggggatcc tggctgccgg tcagagctcc accatgactg gaacctattc tggccagttt
     1321 gtcatggagg gattcctgaa cctaaaatgg tcgcgctttg cccgagtgat cctgacccgg
     1381 tctatcgcca tcatccccac cctgctcgtc gctgtcttcc aggatgtgga gcacctaacg
     1441 gggatgaatg acttcctgaa tgtcctgcag agcttacagc tcccctttgc tctcataccc
     1501 atcctcacgt tcacaagcct gcggccagtg atgagtgagt tttccaatgg aataggctgg
     1561 aggattgccg gtggcatcct ggtcctgatc gtctgctcca tcaacatgta ctttgtagtg
     1621 gtttatgtcc aggagctagg gcatgtggca ctctatgtgg tggctgcagt ggttagcgtg
     1681 gcttatttga cctttgtgtt ctacttgggt tggcagtgtt tgattgcatt gggtctgtct
     1741 ttcctggact gtggacgctc gtaccgcctg ggactgaccg ctcagcctga actctatctt
     1801 ctgaacaccg tggatgctga ctcagtggtg tccagatgac taacagccca ggagacctta
     1861 agaacacttt ctctaagccc tttcgggcca agtgcctgtt agcaaatccc ttagttcgga
     1921 ggtgagcttg ttcaaaggtt tttgaacaaa ggagaagttc tcctcacgga ctcagggtaa
     1981 aagctgacag ttgcgtatgg gtcagagacc cttcggaggc agtgggcctg tggcagctgt
     2041 gcagtccctc aggcttgtgt ctgcacttct ggggtttaaa gtgtatcgat atgtatttat
     2101 gtgaacccaa acgtgccagt ttgggtaaga ttttaaggtt ataaaacttg atagattttt
     2161 acaaatgcct ttcaggtgag ttaggtcttt gttctatttg ggtaacaaat gagaaaggga
     2221 cctttctgac gatgaacttc cccaaaataa ctcttgaagc gctgctttac cacagtgtgt
     2281 gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcgcgcgtgc ctgcctgctt ctgcttgtgg
     2341 gggccacggg ttggcatcag gtgtctccag tctctaccac agtgtgtgtg tgtgtgtgtg
     2401 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgcgtgcgcg agtgcctgcc tgcttctgct
     2461 tgtgggggcc acgggttggc atcaggtgtc tccagtctct ctctgaacat ggagttcagt
     2521 gtgtgactga gctagccagc cagtaagttc aaggatcctc ctggcctcgc gccccaacac
     2581 tgtggtccca gatcagctgc tacgcccaga ttttacacag tgctggggac ccaaactcag
     2641 gtcttcctgg acagcaggta ctttaccaac tttccccctt tgtctctaca tcaagtgtgt
     2701 ctgtggctgt tgttccgttg gtgtctgtcc acctgccctt tcttcccact ctacgcgctg
     2761 acactccaca cactgtcctc accctgtctc taagggccgc ccgtatgtgt accgctgagt
     2821 gtcctgagga atttaagcat tttaaggtgt gtgttagcag aggtctgagg ctcgcactcg
     2881 atgggaacac agaccatcag tttcacttta agcaccgaca ctccaaacgc gcgtgtgcag
     2941 gtagtgaagc tttttggttt ttgctttaat cccaaccaga aataatcatc cttgttataa
     3001 taaagttcat gcttaatagc taaaaaaaaa aaaaaaaaaa a
//
