LOCUS       NM_001177899            2142 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus src family associated phosphoprotein 1 (Skap1),
            transcript variant 3, mRNA.
ACCESSION   NM_001177899
VERSION     NM_001177899.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Smith,X., Taylor,A. and Rudd,C.E.
  TITLE     T-cell immune adaptor SKAP1 regulates the induction of
            collagen-induced arthritis in mice
  JOURNAL   Immunol Lett 176, 122-127 (2016)
   PUBMED   27181093
  REMARK    GeneRIF: this study shows that Skap1-deficient (skap1-/-) mice are
            highly resistant to the induction of collagen-induced arthritis
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Lim,D., Lu,Y. and Rudd,C.E.
  TITLE     Non-cleavable talin rescues defect in the T-cell conjugation of
            T-cells deficient in the immune adaptor SKAP1
  JOURNAL   Immunol Lett 172, 40-46 (2016)
   PUBMED   26905930
  REMARK    GeneRIF: SKAP1 can affect the function of talin in T-cells needed
            for optimal T-cell/dendritic cell conjugation
REFERENCE   3  (bases 1 to 2142)
  AUTHORS   Li,C., Li,W., Xiao,J., Jiao,S., Teng,F., Xue,S., Zhang,C.,
            Sheng,C., Leng,Q., Rudd,C.E., Wei,B. and Wang,H.
  TITLE     ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+
            cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy
  JOURNAL   EMBO Mol Med 7 (6), 754-769 (2015)
   PUBMED   25851535
  REMARK    GeneRIF: In this study, the authors identified that the ADAP-SKAP55
            signaling module reduced CD8(+) cytotoxic T lymphocyte cytotoxicity
            and enhanced PD-1 expression in a Fyn-, Ca(2+)-, and
            NFATc1-dependent manner.
REFERENCE   4  (bases 1 to 2142)
  AUTHORS   Ayoub,E., Hall,A., Scott,A.M., Chagnon,M.J., Miquel,G., Halle,M.,
            Noda,M., Bikfalvi,A. and Tremblay,M.L.
  TITLE     Regulation of the Src kinase-associated phosphoprotein 55 homologue
            by the protein tyrosine phosphatase PTP-PEST in the control of cell
            motility
  JOURNAL   J Biol Chem 288 (36), 25739-25748 (2013)
   PUBMED   23897807
REFERENCE   5  (bases 1 to 2142)
  AUTHORS   Liang,Y., Cucchetti,M., Roncagalli,R., Yokosuka,T., Malzac,A.,
            Bertosio,E., Imbert,J., Nijman,I.J., Suchanek,M., Saito,T.,
            Wulfing,C., Malissen,B. and Malissen,M.
  TITLE     The lymphoid lineage-specific actin-uncapping protein Rltpr is
            essential for costimulation via CD28 and the development of
            regulatory T cells
  JOURNAL   Nat Immunol 14 (8), 858-866 (2013)
   PUBMED   23793062
REFERENCE   6  (bases 1 to 2142)
  AUTHORS   Schneider,H., Wang,H., Raab,M., Valk,E., Smith,X., Lovatt,M.,
            Wu,Z., Maqueira-Iglesias,B., Strebhardt,K. and Rudd,C.E.
  TITLE     Adaptor SKAP-55 binds p21 activating exchange factor RasGRP1 and
            negatively regulates the p21-ERK pathway in T-cells
  JOURNAL   PLoS One 3 (3), e1718 (2008)
   PUBMED   18320039
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2142)
  AUTHORS   Wang,H., Liu,H., Lu,Y., Lovatt,M., Wei,B. and Rudd,C.E.
  TITLE     Functional defects of SKAP-55-deficient T cells identify a
            regulatory role for the adaptor in LFA-1 adhesion
  JOURNAL   Mol Cell Biol 27 (19), 6863-6875 (2007)
   PUBMED   17646386
  REMARK    GeneRIF: Results identify a clear effector role for SKAP-55 in
            LFA-1 adhesion in peripheral T cells and demonstrate that
            dependency on SKAP-55 and ADAP differs among T cells and differs
            with the strength of the TCR signal.
REFERENCE   8  (bases 1 to 2142)
  AUTHORS   Menasche,G., Kliche,S., Chen,E.J., Stradal,T.E., Schraven,B. and
            Koretzky,G.
  TITLE     RIAM links the ADAP/SKAP-55 signaling module to Rap1, facilitating
            T-cell-receptor-mediated integrin activation
  JOURNAL   Mol Cell Biol 27 (11), 4070-4081 (2007)
   PUBMED   17403904
REFERENCE   9  (bases 1 to 2142)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   10 (bases 1 to 2142)
  AUTHORS   Jo,E.K., Wang,H. and Rudd,C.E.
  TITLE     An essential role for SKAP-55 in LFA-1 clustering on T cells that
            cannot be substituted by SKAP-55R
  JOURNAL   J Exp Med 201 (11), 1733-1739 (2005)
   PUBMED   15939789
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK040068.1, BY723539.1, AK137942.1 and BC150764.1.
            
            Transcript Variant: This variant (3) lacks an alternate, in-frame
            exon, compared to variant 1. The resulting protein (isoform 3) is
            shorter when it is compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145402.1, AK169825.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-472               AK040068.1         1-472
            473-660             BY723539.1         453-640
            661-931             AK137942.1         629-899
            932-1997            BC150764.1         861-1926
            1998-2142           AK137942.1         2014-2158
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 59.93 cM"
     gene            1..2142
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /note="src family associated phosphoprotein 1"
                     /db_xref="GeneID:78473"
                     /db_xref="MGI:MGI:1925723"
     exon            1..160
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1134
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /note="isoform 3 is encoded by transcript variant 3; src
                     kinase-associated phosphoprotein 1; ortholog of human src
                     family associated phosphoprotein 1 SCAP1"
                     /codon_start=1
                     /product="src kinase-associated phosphoprotein 1 isoform
                     3"
                     /protein_id="NP_001171370.1"
                     /db_xref="CCDS:CCDS48891.1"
                     /db_xref="GeneID:78473"
                     /db_xref="MGI:MGI:1925723"
                     /translation="MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRG
                     FQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGIEDILRGA
                     QELDSVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGY
                     SVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIP
                     FEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPVDYA
                     DYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSVIGIVPKDYLTTAF
                     EMEGI"
     exon            161..266
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            267..292
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            293..394
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            395..472
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            473..556
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            557..681
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            682..745
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            746..931
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1032
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1141
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..2142
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1437..1442
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /note="hexamer: AATAAA"
     polyA_site      1462
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /note="major polyA site"
     regulatory      1641..1646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
                     /note="hexamer: AATAAA"
     polyA_site      1663
                     /gene="Skap1"
                     /gene_synonym="1700091G21Rik; Scap1; Skap-55"
ORIGIN      
        1 ccaggcgaca gccgaggtac aggtgcctgg ggtgcagctc gctgcgcccg ccgtccgagc
       61 aggcacacgt cccttatccc gccttctgct gcggccgccc actcgcttcg ccaaatgcag
      121 gctgtcgccc tcccggagga gatctgttgg ctcctggaag ataccgaaga cttcctggca
      181 gaaggcttgc agaatgagaa cctcagccct ggggcacagg atcagagagc ccacattctt
      241 cggggtttcc agcaaatcaa atccaggtac tgttgggatt ttcagcccca aggaggtgac
      301 cttggccagg acggctctga tgacaatctg agtgggactc acggcccacc cctcacgtca
      361 gaagcatcct tttggtcaga ttatcaggac gagggaatag aagacatctt aagaggggct
      421 caagagctgg atagcgtcat caagcaaggg tacctggaga agaaaagcaa agatcatagc
      481 ttctttgggt cggagtggca gaagcgctgg tgtgttatca gcagagggct cttcctctac
      541 tatgctaatg agaaaagcaa gcagcccaaa gggaccttcc tcattaaggg ctacagtgtc
      601 cggatggccc ctcacctgcg aaaagattcc aagaaagaat cctgctttga actgatctcc
      661 caggataggc gcagctatga gtttacagct tctagtccag ctgaagccag agactgggtg
      721 gatcaaataa gtttcttgtt aaaagatctt agctccttaa ccattccctt cgaagaggag
      781 gaggaggaag aggaggaaga agagaaagaa gaggaagaaa tgtacaatga cgtcgatggc
      841 tttgactccc caaggtctgg ctcccagtgc agagccatgg ccttgcctga gcctactgag
      901 aaagaggagg acatttatga agtcctacca gtggactatg ccgattatta ccagggccta
      961 tgggattgcc atggtgacca gccggatgaa ctgtccttcc aacggggtga cctcatccgc
     1021 attctgagca aggagtacaa catgtacggc tggtgggtag gagagctgaa cagcgtcatt
     1081 ggaattgttc caaaggacta tctcaccacc gcctttgaaa tggaaggaat atgaagccca
     1141 gggaacatat tctcagacca tcagaaactc ccgtccccta ctctggccat ggatccttct
     1201 cttccctgaa tggagccaag actctgctgc ttcctgtgac tttaagccac tgataagatg
     1261 agtaggaaga agacagttca tcagacctgt ttctcaagca gccagcttct agcttcctgc
     1321 cctctctgtg tccccacatc cataagcctt tcctacaagc ctgctacaaa gaagacagca
     1381 acgaaggaaa tcatgggagg aagaggttac tgatgggttg tggcttgcaa agcagaaata
     1441 aagtctggat atgtttcctt gccttgtatg gtggggctga attcctttct gacataatat
     1501 cacgggctgg cgagatggtt ccatgggtaa gagcacctgc tgccaggcct gatgacctga
     1561 gttcaggccc caggacccac atgttgtcct ctgacctccg tgcatgtacc gtgccacatg
     1621 tatacccaca cacatgaata aataaaactg aataataagc aaatgttaag ttttaatagt
     1681 atcaaaatgc cactgctagg tattcttgcc atctgcaaag tgggaaggga cagtcacagc
     1741 aaaagtgaaa gcccagacag ggaaggggag attgactgtc ttcagtccca ctgatgggac
     1801 agacatctct ggaactcagg acagctagtg cccttctggt cagtgtctca gactgccttt
     1861 ggttggctac ttggttgttt cttctgtgat gaacctctga gacacagaag caaggcctga
     1921 aaatgagcat gctggagagc ccctctgtgg gcattgtggt ctccagtgat ggaatcccag
     1981 atggactgag ggcatggggt ggggtcgctc tctaaaacag ggagtcctgc tcctttaaca
     2041 gtcacctgca cctagttctg cacgttgaca aggcaacaga tcagtggttg ggaacacaaa
     2101 ccccagcgct agacaacacg ggaataaatc tcatttttcc tt
//
