LOCUS       NM_025377               1349 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus spindle and kinetochore associated complex subunit 2
            (Ska2), mRNA.
ACCESSION   NM_025377
VERSION     NM_025377.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Hartmann,J., Bajaj,T., Otten,J., Klengel,C., Ebert,T.,
            Gellner,A.K., Junglas,E., Hafner,K., Anderzhanova,E.A., Tang,F.,
            Missig,G., Rexrode,L., Trussell,D.T., Li,K.X., Pohlmann,M.L.,
            Mackert,S., Geiger,T.M., Heinz,D.E., Lardenoije,R., Dedic,N.,
            McCullough,K.M., Prochnicki,T., Rhomberg,T., Martinelli,S.,
            Payton,A., Robinson,A.C., Stein,V., Latz,E., Carlezon,W.A. Jr.,
            Hausch,F., Schmidt,M.V., Murgatroyd,C., Berretta,S., Klengel,T.,
            Pantazopoulos,H., Ressler,K.J. and Gassen,N.C.
  TITLE     SKA2 regulated hyperactive secretory autophagy drives
            neuroinflammation-induced neurodegeneration
  JOURNAL   Nat Commun 15 (1), 2635 (2024)
   PUBMED   38528004
  REMARK    GeneRIF: SKA2 regulated hyperactive secretory autophagy drives
            neuroinflammation-induced neurodegeneration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1349)
  AUTHORS   Xiong,Y., Li,L., Wang,N., Wang,F., Chen,Z., Han,L., Jiang,S. and
            Gu,Y.
  TITLE     Circ_0001387 regulates SKA2 to accelerate breast cancer progression
            through miR-136-5p
  JOURNAL   Thorac Cancer 14 (18), 1707-1718 (2023)
   PUBMED   37132052
  REMARK    GeneRIF: Circ_0001387 regulates SKA2 to accelerate breast cancer
            progression through miR-136-5p.
REFERENCE   4  (bases 1 to 1349)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1349)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 1349)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 1349)
  AUTHORS   Zhang,Q.H., Qi,S.T., Wang,Z.B., Yang,C.R., Wei,Y.C., Chen,L.,
            Ouyang,Y.C., Hou,Y., Schatten,H. and Sun,Q.Y.
  TITLE     Localization and function of the Ska complex during mouse oocyte
            meiotic maturation
  JOURNAL   Cell Cycle 11 (5), 909-916 (2012)
   PUBMED   22336914
  REMARK    GeneRIF: The Skas subunit was only localized on spindle
            microtubules from the Pro-MI to MII stages in mouse oocyte meiosis
            and knockdown by Morpholino injection into mouse oocytes resulted
            in spindle movement defects and enlarged polar bodies.
REFERENCE   8  (bases 1 to 1349)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1349)
  AUTHORS   Rice,L., Waters,C.E., Eccles,J., Garside,H., Sommer,P., Kay,P.,
            Blackhall,F.H., Zeef,L., Telfer,B., Stratford,I., Clarke,R.,
            Singh,D., Stevens,A., White,A. and Ray,D.W.
  TITLE     Identification and functional analysis of SKA2 interaction with the
            glucocorticoid receptor
  JOURNAL   J Endocrinol 198 (3), 499-509 (2008)
   PUBMED   18583474
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL713917.6.
            
            On Apr 29, 2007 this sequence version replaced NM_025377.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017811.1, AK013370.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AL713917.6         122175-122218
            45-131              AL713917.6         129018-129104
            132-308             AL713917.6         130582-130758
            309-1349            AL713917.6         134848-135888
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.91 cM"
     gene            1..1349
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="spindle and kinetochore associated complex subunit
                     2"
                     /db_xref="GeneID:66140"
                     /db_xref="MGI:MGI:1913390"
     exon            1..44
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..374
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="spindle and kinetochore-associated protein 2;
                     family with sequence similarity 33, member A"
                     /codon_start=1
                     /product="SKA complex subunit 2"
                     /protein_id="NP_079653.1"
                     /db_xref="CCDS:CCDS25209.1"
                     /db_xref="GeneID:66140"
                     /db_xref="MGI:MGI:1913390"
                     /translation="MEAEVDKLELMFQKADSDLDYLQYRLEYEVKTNHPHSAGEKNAV
                     TVLKELSAIKSRYQALCARFKAVSVEQKETKSCICATLNKTMTMIQELQKQTNLELTL
                     LTEEEKAATEPLKSHMPD"
     exon            45..131
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /inference="alignment:Splign:2.1.0"
     exon            132..308
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /inference="alignment:Splign:2.1.0"
     exon            309..1349
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      564..569
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="hexamer: AATAAA"
     polyA_site      585
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="major polyA site"
     regulatory      1212..1217
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="hexamer: AATAAA"
     polyA_site      1239
                     /gene="Ska2"
                     /gene_synonym="1110001A07Rik; Fam33a"
                     /note="major polyA site"
ORIGIN      
        1 aactattcaa tatggaggcg gaggtcgata agctggaact gatgttccag aaagctgatt
       61 ctgatttgga ttaccttcaa tataggctgg aatatgaagt caagactaat cacccacatt
      121 cagcaggaga gaaaaatgca gttacagttt taaaggaatt atcagcgata aagtctcgct
      181 atcaagcttt atgtgcacgc tttaaggcag tttctgttga gcaaaaggag accaagagct
      241 gcatttgtgc tactttgaac aagacaatga ccatgataca agaactacaa aagcaaacaa
      301 acctggagct aactctactg actgaagaag agaaagctgc aacagagcca ttaaaatctc
      361 atatgccgga ctgataaaaa atgttaaaga ggaaggctgg tggaaagaag accaactgca
      421 gagattgggc gagacgtcta gaggcggggc tgatgaggaa gcctgacatt tcccttgacc
      481 gagaggatcg tgatgcattt cagatgagga aattgtaaga agcgtagatg tcctgtgact
      541 gaaaaccaaa aggatgggat ggaaataaag caatgtaaat actgataccc agcattcatt
      601 ttaggcaatg aaatccctta gtactacttt tttttttatt ttctgttttc tttttgtata
      661 gtgttggcat aattttagat gttttgggat tttgtaaaat aatatttatg tacattttgt
      721 tcagtatgta aatgggcaaa atacttgtat aaaaattcag gtgttgtttg ctcttttgag
      781 acaaggtttc actatgtagc tctggctggc cctggagctc actatgtaga ccagaccagc
      841 tctgaatcct aaagatgcct gcctttgcta tcacgctggg cattgtctgt ctgtctgtct
      901 gtgacaaggt cttccatatc ccaggctggc ctcagatttg gtgtcaatag ggataatatt
      961 tgacattggt acttcctgag tgctgggata cccagtgtgc agaagcacgt ctggtttgtg
     1021 tagtaccagc tagcacggct cagctgagcc acatcccaac cctgtatttt tttttcttta
     1081 atgtacatta cagttaataa cacacttact tgtatgtata tactgtccag ttttcccaaa
     1141 atctaacaaa ttaataggat gttttgtgag aacttttatt ttttttttcc tttttcccat
     1201 tgcttgctag taataaaaca tcattagttt gttttcaaat gctgttttag actttctatt
     1261 ggtgttacaa taatatgtta tctttcagtg tagttttgta tttgtttatt ctatatgaat
     1321 gacagactaa aatcactgac acatctgct
//
