LOCUS       NM_001164355            2605 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus spindle and kinetochore associated complex subunit 1
            (Ska1), transcript variant 2, mRNA.
ACCESSION   NM_001164355
VERSION     NM_001164355.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   3  (bases 1 to 2605)
  AUTHORS   Li,J., Xuan,J.W., Khatamianfar,V., Valiyeva,F., Moussa,M.,
            Sadek,A., Yang,B.B., Dong,B.J., Huang,Y.R. and Gao,W.Q.
  TITLE     SKA1 over-expression promotes centriole over-duplication,
            centrosome amplification and prostate tumourigenesis
  JOURNAL   J Pathol 234 (2), 178-189 (2014)
   PUBMED   24827423
  REMARK    GeneRIF: Ska1 over-expression promotes tumourigenesis
REFERENCE   4  (bases 1 to 2605)
  AUTHORS   Zhang,Q.H., Qi,S.T., Wang,Z.B., Yang,C.R., Wei,Y.C., Chen,L.,
            Ouyang,Y.C., Hou,Y., Schatten,H. and Sun,Q.Y.
  TITLE     Localization and function of the Ska complex during mouse oocyte
            meiotic maturation
  JOURNAL   Cell Cycle 11 (5), 909-916 (2012)
   PUBMED   22336914
  REMARK    GeneRIF: The Ska1 subunit was only localized on spindle
            microtubules from the Pro-MI to MII stages in mouse oocyte meiosis
            and knockdown by Morpholino injection into mouse oocytes resulted
            in spindle movement defects and enlarged polar bodies.
REFERENCE   5  (bases 1 to 2605)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2605)
  AUTHORS   Rines,D.R., Gomez-Ferreria,M.A., Zhou,Y., DeJesus,P., Grob,S.,
            Batalov,S., Labow,M., Huesken,D., Mickanin,C., Hall,J.,
            Reinhardt,M., Natt,F., Lange,J., Sharp,D.J., Chanda,S.K. and
            Caldwell,J.S.
  TITLE     Whole genome functional analysis identifies novel components
            required for mitotic spindle integrity in human cells
  JOURNAL   Genome Biol 9 (2), R44 (2008)
   PUBMED   18302737
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ069399.1, AK021226.1, AK013233.1 and AC133186.4.
            
            Transcript Variant: This variant (2) uses a different splice site
            in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the
            same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK021226.1, AK013233.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               CJ069399.1         1-165
            166-541             AK021226.1         130-505
            542-1138            AK013233.1         504-1100
            1139-2605           AC133186.4         145410-146876       c
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 50.67 cM"
     gene            1..2605
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="spindle and kinetochore associated complex subunit
                     1"
                     /db_xref="GeneID:66468"
                     /db_xref="MGI:MGI:1913718"
     exon            1..225
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            226..321
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..1001
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="spindle and kinetochore-associated protein 1"
                     /codon_start=1
                     /product="SKA complex subunit 1"
                     /protein_id="NP_001157827.1"
                     /db_xref="CCDS:CCDS29341.1"
                     /db_xref="GeneID:66468"
                     /db_xref="MGI:MGI:1913718"
                     /translation="MDSELEDLCSYVNEKIGNIKKILSIRNLGQDPALKTTLSKIGDE
                     IIAVNELLNKFELEIQYQEQTNSSLKELCESLREECEDVEHLKEHVPPHLPQVTATQS
                     LVHKPEPDPKESDKAEEPGLPKKPPREQRIIKEMQFITMDEFSDVPAYMKSRLTYCQI
                     NDIIKEINKAVVSKYKIMHQPKASMSSVKRNLYQRFINEETKDTKGHHFIVEADIKEF
                     TALKVDKRFYVIMHILRHCHRLSEVRGGGLTRYVIT"
     exon            322..446
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            447..544
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            545..682
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..852
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            853..2605
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1107..1112
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="hexamer: AGTAAA"
     regulatory      1119..1124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="hexamer: GATAAA"
     polyA_site      1129
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="major polyA site"
     polyA_site      1142
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
     regulatory      1734..1739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
                     /note="hexamer: ATTACA"
     polyA_site      1755
                     /gene="Ska1"
                     /gene_synonym="2810433K01Rik"
ORIGIN      
        1 tttccctagc ggagtctgcg ctgttggagg aacgcctttg tgcgcctgcg cggccgcgga
       61 tccggcgggc tgtttgaatt tgggctctgg aagctggcgt ctggatttgg cgtttcggtc
      121 gtggaccggt gcgcgcttgg tccccagccg tagcgcaggc tcttcgctcc agactcccgc
      181 aaggtcgctg gctccgaagc cgcccgaaga ggcatcgctt agcagacgct taaaggatgg
      241 actcagagct agaagactta tgctcttatg ttaatgagaa gattggaaat attaagaaaa
      301 ttctgtcgat aagaaatctt ggccaggacc ctgccttgaa gaccacctta agtaaaatag
      361 gagatgagat cattgcagta aatgagcttc taaataaatt tgaattggaa attcagtatc
      421 aagaacaaac taacagttca ctcaaggagc tctgtgaatc tcttagagaa gagtgtgaag
      481 atgtggaaca tctcaaagaa cacgtccctc cccatctgcc tcaagtgacg gcgacccaga
      541 gcttagttca caagccagag ccggatccta aagagtcaga caaagctgag gagcctggac
      601 tgccgaagaa gccccccaga gagcagagga ttatcaagga aatgcaattt ataactatgg
      661 atgaattcag tgacgttcct gcgtacatga aatcccgttt aacatattgt caaattaatg
      721 atattattaa ggaaattaac aaggcagtag ttagcaaata taagattatg catcaaccaa
      781 aagcatctat gagctctgtg aagagaaatc tctaccagag atttattaat gaagaaacga
      841 aggacacgaa aggtcatcac ttcatcgtgg aagctgacat aaaggaattc acggctttga
      901 aagttgacaa gaggttttat gtgattatgc acattttgcg gcattgccac agactgtcag
      961 aggtccgagg gggaggactt acccgatacg tcatcacctg aggcttttga agactcggga
     1021 ctgattggca gcagggtatc gaggggggtc ttgaaatttc atgtatgttt ttagcttctc
     1081 catttcctgg ccttgtttgt tacagaagta aaactttgga taaagcatat atattttcaa
     1141 aacgtactgc ctttaggaac gtgggtgaag ctggagctca caagcacagt aagccagtta
     1201 ggaagactga ctcgtgtgga acctgtgtgt gtataggagt cgggggatca tgaggccagg
     1261 aggaggatct ggagaagtgc ggagaaaaga ggaacagaga aggcagtgga ccgctgtgat
     1321 agagtaggag gaaagaccgt ctgggaacag ccaggggaag ggtggcaagc acttgggcag
     1381 gaggtggatc aggataaagt gcgatgccac aagatataaa aatgcagtaa caggactcat
     1441 ttctttgtgt gctaacataa aaattaattt taaaaatccc ttcctttcag caaatattca
     1501 ggcactgccc tgtcaagcct ctgcatgagt tgtataatgg acagcgtgtc tagttctgtt
     1561 aagtgcagat accatgagca tagtatgagg tcgggaaaga tggtgaatac ccaggtcctc
     1621 tgtggcgtaa agtctgatta ctgtttttcc acgtgcccat agagaacagc ttgcctaaaa
     1681 tgcattcagt ataagctgga ggctctgaag tacattgaga tactgtcaca tgtattacat
     1741 atgatatgta ttacagcttt acgtctggtt ccttgttcag ggtttcgaaa aatccttgta
     1801 acgtcctaag tggtaagggt gcgtcttgtg ttgaactcag ttcttgactg tagctttcaa
     1861 acgcctttga aacatttttc tgatcagaac atcttttgct cagattctag tgaggtgatt
     1921 cttgggcgcc ttcaaggcgg gcactagtta ccaaaatact aagccatgac taggaagttg
     1981 cagtttggag ccattctcct gctcccgggg agggagacac ccttgttgca catccggcct
     2041 cagcaactca gttacagggg gagatttctt gtctctaaag ccaaaaccac ttggtcaaag
     2101 ctgtcaagtt ctgctgcctg atgaggctag agatgtttgg tcatgccgac aggcttcgtc
     2161 ctttttaaaa ttaatttaaa aaaatcactg ctctgggcct ccatgcaggg acacccctgt
     2221 cacacatctg gcctcagcaa cttagttgca gagagagaga tttcttgact ctaaagccag
     2281 aaccacatgg ctaaagctgc caagttctgt tgctttctgg ggctggagta tggccctgtt
     2341 gttcagttag atcttcacca gcggtctggt tttgatagtt tccttcactg cctaagcatg
     2401 gctgtcttgg aacttgctct gtagaccagg ctgctcttga actcagagat ctgcataccc
     2461 ctgtcttcta agtgctggaa ctaaaggcgt gtaccaccca ccacacctgg acctaaactt
     2521 tcccttaatt ccttttcatt ttacatcgtt gtaagcatgg ccactgtgcc tcacgatatg
     2581 gattaaaggt tcgtgttcat ccaca
//
