LOCUS       NM_001081137            5953 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus sucrase isomaltase (Sis), mRNA.
ACCESSION   NM_001081137 XM_006502044 XM_143332
VERSION     NM_001081137.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Senftleber,N.K., Skott Pedersen,K., Schnoor Jorgensen,C.,
            Pedersen,H., Bjerg Christensen,M.M., Kabel Madsen,E., Andersen,K.,
            Jorsboe,E., Gillum,M.P., Frost,M.B., Hansen,T. and Jorgensen,M.E.
  TITLE     The effect of sucrase-isomaltase deficiency on metabolism, food
            intake and preferences: protocol for a dietary intervention study
  JOURNAL   Int J Circumpolar Health 82 (1), 2178067 (2023)
   PUBMED   38113483
  REMARK    GeneRIF: The effect of sucrase-isomaltase deficiency on metabolism,
            food intake and preferences: protocol for a dietary intervention
            study.
REFERENCE   3  (bases 1 to 5953)
  AUTHORS   Andersen,M.K., Skotte,L., Jorsboe,E., Polito,R., Staeger,F.F.,
            Aldiss,P., Hanghoj,K., Waples,R.K., Santander,C.G., Grarup,N.,
            Dahl-Petersen,I.K., Diaz,L.J., Overvad,M., Senftleber,N.K.,
            Soborg,B., Larsen,C.V.L., Lemoine,C., Pedersen,O., Feenstra,B.,
            Bjerregaard,P., Melbye,M., Jorgensen,M.E., Faergeman,N.J., Koch,A.,
            Moritz,T., Gillum,M.P., Moltke,I., Hansen,T. and Albrechtsen,A.
  TITLE     Loss of Sucrase-Isomaltase Function Increases Acetate Levels and
            Improves Metabolic Health in Greenlandic Cohorts
  JOURNAL   Gastroenterology 162 (4), 1171-1182 (2022)
   PUBMED   34914943
REFERENCE   4  (bases 1 to 5953)
  AUTHORS   Yanai,H., Atsumi,N., Tanaka,T., Nakamura,N., Komai,Y., Omachi,T.,
            Tanaka,K., Ishigaki,K., Saiga,K., Ohsugi,H., Tokuyama,Y.,
            Imahashi,Y., Ohe,S., Hisha,H., Yoshida,N., Kumano,K., Kon,M. and
            Ueno,H.
  TITLE     Intestinal stem cells contribute to the maturation of the neonatal
            small intestine and colon independently of digestive activity
  JOURNAL   Sci Rep 7 (1), 9891 (2017)
   PUBMED   28860595
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5953)
  AUTHORS   Blais,S., Boudreau,F., Beaulieu,J.F. and Asselin,C.
  TITLE     CCAAT/enhancer binding protein isoforms expression in the colon of
            neonatal mice
  JOURNAL   Dev Dyn 204 (1), 66-76 (1995)
   PUBMED   8563027
REFERENCE   6  (bases 1 to 5953)
  AUTHORS   Quezada-Calvillo,R., Markowitz,A.J., Traber,P.G. and Underdown,B.J.
  TITLE     Murine intestinal disaccharidases: identification of structural
            variants of sucrase-isomaltase complex
  JOURNAL   Am J Physiol 265 (6 Pt 1), G1141-G1149 (1993)
   PUBMED   8279565
REFERENCE   7  (bases 1 to 5953)
  AUTHORS   Birkenmeier,E.H., Rowe,L.B., Leeper,L.L. and Henning,S.J.
  TITLE     The sucrase-isomaltase structural gene (Si-s) and a regulatory gene
            (Si-r) are closely linked to esterase-26 (Es-26) on mouse
            chromosome 3
  JOURNAL   Mamm Genome 4 (9), 531-536 (1993)
   PUBMED   8118103
REFERENCE   8  (bases 1 to 5953)
  AUTHORS   Traber,P.G., Wu,G.D. and Wang,W.
  TITLE     Novel DNA-binding proteins regulate intestine-specific
            transcription of the sucrase-isomaltase gene
  JOURNAL   Mol Cell Biol 12 (8), 3614-3627 (1992)
   PUBMED   1378530
REFERENCE   9  (bases 1 to 5953)
  AUTHORS   Broyart,J.P., Hugot,J.P., Perret,C. and Porteu,A.
  TITLE     Molecular cloning and characterization of a rat intestinal
            sucrase-isomaltase cDNA. Regulation of sucrase-isomaltase gene
            expression by sucrose feeding
  JOURNAL   Biochim Biophys Acta 1087 (1), 61-67 (1990)
   PUBMED   2400788
REFERENCE   10 (bases 1 to 5953)
  AUTHORS   James,P.S., Smith,M.W., Butcher,G.W., Brown,D. and Lund,E.K.
  TITLE     Evidence for a possible regulatory gene (Suc-1) controlling sucrase
            expression in mouse intestine
  JOURNAL   Biochem Genet 24 (3-4), 169-181 (1986)
   PUBMED   3729924
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164406.5 and AC102399.11.
            
            On or before Nov 4, 2017 this sequence version replaced
            XM_006502044.1, NM_001081137.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU937530.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849377 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC164406.5         93234-93296         c
            64-181              AC164406.5         92182-92299         c
            182-288             AC164406.5         91012-91118         c
            289-406             AC164406.5         86446-86563         c
            407-516             AC164406.5         85691-85800         c
            517-668             AC164406.5         83546-83697         c
            669-840             AC164406.5         81560-81731         c
            841-940             AC164406.5         78549-78648         c
            941-1053            AC164406.5         77864-77976         c
            1054-1179           AC164406.5         77014-77139         c
            1180-1311           AC164406.5         75142-75273         c
            1312-1431           AC164406.5         74935-75054         c
            1432-1545           AC164406.5         72854-72967         c
            1546-1630           AC164406.5         72009-72093         c
            1631-1748           AC164406.5         70546-70663         c
            1749-1920           AC164406.5         68940-69111         c
            1921-2037           AC164406.5         66399-66515         c
            2038-2192           AC164406.5         64032-64186         c
            2193-2277           AC164406.5         62619-62703         c
            2278-2334           AC164406.5         61292-61348         c
            2335-2459           AC164406.5         59518-59642         c
            2460-2548           AC164406.5         57480-57568         c
            2549-2598           AC164406.5         54816-54865         c
            2599-2769           AC164406.5         53982-54152         c
            2770-2928           AC164406.5         53596-53754         c
            2929-3135           AC164406.5         50824-51030         c
            3136-3290           AC164406.5         50381-50535         c
            3291-3459           AC164406.5         48254-48422         c
            3460-3556           AC164406.5         46785-46881         c
            3557-3669           AC164406.5         46568-46680         c
            3670-3795           AC164406.5         46355-46480         c
            3796-3924           AC164406.5         44917-45045         c
            3925-4035           AC164406.5         43961-44071         c
            4036-4098           AC164406.5         42597-42659         c
            4099-4215           AC164406.5         40008-40124         c
            4216-4303           AC164406.5         38623-38710         c
            4304-4442           AC164406.5         37276-37414         c
            4443-4576           AC164406.5         35799-35932         c
            4577-4611           AC164406.5         34601-34635         c
            4612-4728           AC164406.5         34400-34516         c
            4729-4734           AC164406.5         32658-32663         c
            4735-4877           AC102399.11        168424-168566       c
            4878-4962           AC102399.11        165742-165826       c
            4963-5019           AC102399.11        165603-165659       c
            5020-5144           AC102399.11        164948-165072       c
            5145-5233           AC102399.11        161760-161848       c
            5234-5283           AC102399.11        153040-153089       c
            5284-5451           AC102399.11        152668-152835       c
            5452-5953           AC102399.11        149899-150400       c
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 32.59 cM"
     gene            1..5953
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /note="sucrase isomaltase"
                     /db_xref="GeneID:69983"
                     /db_xref="MGI:MGI:1917233"
     exon            1..63
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..5520
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /EC_number="3.2.1.10"
                     /note="alpha-glucosidase; sucrase isomaltase, structural"
                     /codon_start=1
                     /product="sucrase-isomaltase, intestinal"
                     /protein_id="NP_001074606.1"
                     /db_xref="CCDS:CCDS38454.1"
                     /db_xref="GeneID:69983"
                     /db_xref="MGI:MGI:1917233"
                     /translation="MAKKKFSGLEISLIVLFIIVTIIAIALVVVLATKVPAVEEVKSP
                     TSTPSPGRCPPEQGEPLNERINCIPEQHPTKAKCEERGCCWRPWNNTIIPWCFFADNH
                     GYTAASVTNDNSGLKATLSRIPSPTLFGEDIKSVLLTTQSQTRNRFRFKLTDPNNKRY
                     EVPHQFVKDGNGIPAADTLYDVKVSENPFSIKVIRKSNNKVLFDTSIGPLVYSNQYLQ
                     ISTRLPSEYIYGFGEHIHKRFRHDLYWKTWPIFTRDEIPGDNNHNLYGHQTFFMGIED
                     NSGKSYGVFLMNSNAMEVFIQPTPIITYRVTGGVLDFYIFLGDTPEQVVQQYQELIGR
                     PAMPAYWNLGFQLSRWNYVSLDKVKEVVRRNREAGIPYDAQVTDIDYMEDKKDFTYDE
                     VAFKGLPEFAQDLHNHGQKYIIILDPAISINKRANGAEYQTYVRGNEQNVWVKESDGT
                     TSLIGEVWPGLTVYPDFTNPRTWEWWANECNLFHQQVEYDGLWIDMNEVSSFIHGSQK
                     GCAPNLLNYPPFTPGILDKIMYSKTLCMDAVQHWGNQYDVHSLYGYSMAIATEKAVEK
                     VFPNKRSFILTRSTFAGSGHHAAHWLGDNTASWEQMEWSITGMLEFGMFGMPLVGADI
                     CGFLANTTEELCRRWMQLGAFYPFSRNHNAEGYAEQDPAFWGADSLLVNTSRHYLTIR
                     YTLLPFLYTLFYRAHAFGETVARPFLHEFYEDPNSWIEDTQFLWGPALLITPVLRPET
                     KYVSAYIPDATWYDYETGEKRPWRKQRVDMYLPEDKIGLHLRGGYIIPTQQPDVTTTA
                     SRKNPLGLIVALDENQAAKGELFWDDGESKDTIEKKIYILYEFSVSNNNLIVNCTHSS
                     YPEGNTLVFKTIKVLGLSATVTAVTVGENDQQMNPHLAFTFDAFNKILSITDLTFNLG
                     KTFIVRWTTQSFSDNEKFTCYPDVGTATEKTCVERGCIWEPVSGLANVPPCYFPSNHN
                     PYLLTSTQKLATGITAELQLNPASARIKLPSNPISTLRVEVKYHKNDMLQFKIYDAHH
                     KRYEVPVPLNIPDTPTSSEENRLYDVEIKENPFGIQVRRRSTGKLIWDSCLPGFAFND
                     QFIQISTRLPSQYLYGFGEAEHTAFKRNLNWHTWGMFTRDQPPGYKLNSYGFHPYYMA
                     LEDEGNAHGVLLQNSNGMDVTFQPTPALTYRTIGGILDFYMFLGPTPEGATKQYHEVI
                     GFPVMPPYWALGFQLCRYGYRNTSEIEQLYNDMKAAQIPYDVQYTDINYMERQLDFTI
                     GERFKTLPQFVEKIRKEGMKYIVILDPAISGNETQPYPAFERGIQKDVFVKWPNTNDI
                     CWAKVWPDLPNITIDETITEDEAVNASRAHVAFPDFFRNSTSEWWTREIYDFYNEKMK
                     FDGLWIDMNEPSSFVNGTVTNKCRNDTLNYPPYFPELTKRNEGLHFRTMCMETEHILS
                     DGSSVLHYDVHNLYGWSQVKPTLDALRNTTGLRGIVISRSTYPTAGRWGGHWLGDNYA
                     NWENLEKSLIGMLEFNLFGIPYVGADICGFFNDSEYHLCARWMQVGAFYPYSRNHNIQ
                     FTRRQDPVSWNETFAQMSKKVLEIRYTLLPYFYTQMHEAHIHGGTVIRPLMHEFFDDK
                     ETWEIYKQFLWGPAFMVTPVIGPFQTAVNGYVPKARWFDYHTGEDIKVRGKLQTFSAP
                     FDTINLHVRGGYILPCQEPAQNTYYSRQKYMKLIVAADDNQTAKGTLFWDDGESIDTY
                     EKNQYTLIEFNLNQKNLTSTVLKDGYRNKSEMKLGSIYVWGKGTTSISQVNLTYGGNE
                     QQLSFTQDEAKEILTIDLKNVTVTLDEPIQISWS"
     exon            64..181
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            182..288
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            289..406
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            407..516
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            517..668
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            669..840
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            841..940
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1053
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1179
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1311
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1431
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1545
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1630
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1748
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1920
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2037
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2192
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2277
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2334
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2335..2459
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2548
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2598
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2769
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2928
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3135
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..3290
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3291..3459
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3556
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3557..3669
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3670..3795
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..3924
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4035
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4036..4098
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4215
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4216..4303
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4304..4442
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4443..4576
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4577..4611
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4612..4728
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4729..4877
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4878..4962
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            4963..5019
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            5020..5144
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            5145..5233
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            5234..5283
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5451
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
     exon            5452..5953
                     /gene="Sis"
                     /gene_synonym="2010204N08Rik; SI; Si-s"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttactttaac agccttgccc aactccagca caacacagca gatactacag gctatgagct
       61 aagatggcaa agaagaaatt cagtggatta gaaatctctc tgattgttct cttcatcata
      121 gtaacgataa tagctatcgc tcttgttgtg gttttggcta caaaggtgcc tgctgtggaa
      181 gaagtaaaaa gtccaacttc aacccctagt cctggaaggt gtccgcctga gcaaggtgaa
      241 cctctcaatg agagaataaa ctgcattcca gaacagcatc caacaaaggc aaaatgtgaa
      301 gagcgtggct gttgctggcg tccatggaac aacactatta ttccttggtg cttctttgca
      361 gataaccatg gctatactgc tgcgtcagtc acgaatgaca acagtggact taaagccaca
      421 ctgagcagaa tcccttctcc cactctattt ggagaagaca ttaaaagcgt cctcctcacg
      481 actcagagtc agacccgtaa tcgtttccgg ttcaagctca cagatccaaa taacaagaga
      541 tacgaagtgc cacatcagtt tgtaaaagat ggaaatggaa tcccagcagc tgacacactg
      601 tatgatgtca aggtctcaga aaacccgttc agcatcaaag tcatcaggaa aagcaacaac
      661 aaagtcttgt ttgataccag tattggtcct ctggtgtact caaaccaata tttgcagatt
      721 tcaacaagac ttccaagtga atatatttat ggctttggag aacatattca taagagattt
      781 cgtcatgact tatactggaa aacatggcca atctttactc gagacgagat tcctggtgat
      841 aataatcata atctctatgg gcatcagaca ttcttcatgg gcattgaaga taactctggc
      901 aagtcctatg gtgttttcct tatgaacagc aatgcaatgg aggttttcat ccagccaaca
      961 ccaataatta cttacagagt tactggtggc gtattagact tttatatatt tcttggagac
     1021 acaccagaac aagttgttca acaatatcaa gagctcattg gacgaccagc aatgccagca
     1081 tattggaacc ttggctttca actgagtcgc tggaattacg tgtctttaga taaagtgaaa
     1141 gaagtagtaa gaagaaaccg ggaagctggc atcccatatg atgcacaagt cactgatatt
     1201 gactacatgg aagacaaaaa ggactttact tatgatgaag tggcatttaa aggacttcct
     1261 gagtttgctc aggatttaca taaccatgga caaaaatata ttatcatcct ggaccctgca
     1321 atctctataa acaaacgtgc aaatggtgcc gaatatcaaa catatgtgag ggggaatgag
     1381 caaaatgttt gggtgaaaga gtcagatgga accacatcac ttattggaga ggtatggcca
     1441 ggattaacag tgtacccaga ttttactaat cccagaacct gggagtggtg ggcaaatgaa
     1501 tgcaatcttt tccatcagca agtggaatat gatggactct ggattgacat gaatgaagtt
     1561 tccagcttta tccacggttc tcagaaagga tgtgctccca atctactgaa ttaccctcct
     1621 tttactcccg gtattcttga caaaatcatg tactccaaaa cgctttgcat ggatgctgtg
     1681 cagcactggg ggaatcagta tgatgtacac agcctgtatg gatacagcat ggctatagcc
     1741 acagagaaag ctgtggaaaa agtttttcct aataagagaa gcttcatcct tacccggtca
     1801 acatttgctg gttcgggaca ccatgctgct cattggcttg gagacaacac agcctcctgg
     1861 gagcagatgg agtggtccat cactggaatg ctggagttcg gcatgttcgg tatgcctttg
     1921 gtaggggcgg acatctgtgg atttttggct aacaccacag aagaactctg cagaagatgg
     1981 atgcaactgg gagcctttta tccattttct agaaaccaca atgctgaagg ctatgcggaa
     2041 caagatcctg ccttttgggg agcagactct cttttggtaa acacatcaag gcactaccta
     2101 accatccgct acactttact tcccttcctc tacactctgt tctatagagc ccacgcgttt
     2161 ggtgagacag ttgcaaggcc atttcttcat gagttttatg aagacccaaa cagctggatt
     2221 gaggacaccc agttcctgtg gggccctgca ctgctgatca ctcctgtttt gagaccagaa
     2281 acaaaatacg tgagtgcata tattcctgat gctacctggt atgattatga aacaggtgaa
     2341 aaaagaccat ggagaaaaca aagggttgac atgtaccttc cagaagataa aataggatta
     2401 catcttagag gaggttatat catccctacc cagcaacctg atgtaacaac aacagcaagc
     2461 cgaaagaatc ctctaggcct tattgttgca ttagatgaaa accaggcagc aaaaggagaa
     2521 ctcttctggg atgatggtga atctaaagat actatagaaa agaagatcta tatattatat
     2581 gagttttcag tttctaataa taacttgatt gttaactgca cacattcatc atatccagag
     2641 ggaaataccc ttgtgtttaa aacaatcaaa gtccttgggc tgagcgccac tgtcacagcg
     2701 gttacagtgg gtgaaaatga tcagcaaatg aaccctcatc ttgctttcac ttttgatgct
     2761 ttcaataaga ttctctcaat tacagatctc acattcaacc tcggcaaaac ctttatagtt
     2821 agatggacga cgcaaagttt ctcagacaat gagaaattca cttgttatcc agatgtaggc
     2881 acagctactg agaaaacttg cgtagagaga ggctgcatat gggaaccggt gagcgggctc
     2941 gccaatgtgc ctccttgcta ctttccttcg aaccacaatc cctatttgtt aacttcaact
     3001 caaaaattgg caacgggcat aacagcagaa cttcagttga atcctgcaag tgctagaatt
     3061 aagcttccat ctaatcccat ctccactctt cgcgtggaag taaaatatca caaaaatgac
     3121 atgctacagt ttaagattta cgatgcacat cataagagat acgaagttcc tgtaccacta
     3181 aatattccag acacccctac aagctctgag gaaaatagac tctatgatgt agaaatcaag
     3241 gaaaatcctt ttggcatcca ggttcgaagg agaagcactg gaaagcttat ctgggattct
     3301 tgcctacctg gatttgcctt caatgatcaa ttcattcaga tatctacacg tctgccatca
     3361 caatatttat atggttttgg ggaagcggaa cacacagcat tcaagcgaaa tttgaattgg
     3421 catacttggg ggatgttcac aagagatcaa cctcctggat ataaacttaa ttcctatgga
     3481 ttccatccgt attacatggc tttggaggat gagggcaatg ctcatggagt cctgttacag
     3541 aacagcaatg gaatggatgt tacattccag ccaacacctg ctctaaccta ccgcacaatt
     3601 ggtggaatct tggattttta catgtttttg ggaccaaccc cagaaggtgc aacaaagcaa
     3661 tatcatgaag taattggctt tccagtcatg ccaccttact gggctctggg attccaacta
     3721 tgtcgttatg gatacagaaa tacttcagag attgagcaac tgtacaatga catgaaagct
     3781 gctcaaatcc cctatgatgt tcagtataca gacatcaatt atatggaaag acaactagac
     3841 ttcacaattg gtgaacgatt caagactctt cctcagtttg ttgaaaaaat aagaaaagag
     3901 ggaatgaaat atattgttat actggatcca gcaatttcag gaaatgaaac ccagccttat
     3961 cctgccttcg aaagaggaat acagaaagat gtttttgtca aatggcctaa cacaaatgac
     4021 atttgctggg caaaggtttg gccagattta cctaatatta caatagatga gactataaca
     4081 gaagatgaag ctgttaacgc ttccagagca catgtagctt ttccagactt ctttaggaat
     4141 tccacatcag agtggtggac aagagaaatt tatgacttct acaatgaaaa aatgaagttt
     4201 gatggcttgt ggattgacat gaatgagcct tccagctttg taaatggaac cgttacaaat
     4261 aaatgcagaa atgatacact aaattatcca ccttattttc cagaactcac aaaaagaaat
     4321 gaggggttac atttccgaac catgtgcatg gaaacagaac atatccttag tgatggatca
     4381 tcagttttgc attatgatgt gcacaattta tatggatggt cacaagttaa acccactctg
     4441 gatgcattac ggaacaccac tggccttcga gggatagtca tttctcggtc cacatatccc
     4501 acagctggtc gatggggagg acactggttg ggagacaact atgcaaactg ggagaatttg
     4561 gaaaaatcac tcattggtat gctggaattt aatctttttg gaataccata tgttggagca
     4621 gatatctgtg gtttctttaa tgactcagaa taccacctct gtgctcgttg gatgcaagtt
     4681 ggagcctttt atccatattc aagaaatcac aacattcaat ttactagaag gcaagatcct
     4741 gtttcctgga atgaaacttt tgctcaaatg tcaaagaaag ttttagaaat cagatacaca
     4801 ttgctgccct atttctacac acagatgcat gaagcccata ttcatggtgg aactgtcatc
     4861 agacccctaa tgcatgagtt ctttgatgat aaagaaacct gggaaatata caagcagttc
     4921 ctgtggggtc cagcttttat ggtgactcct gtcattggac ctttccagac agctgtgaat
     4981 ggctatgttc ctaaggctcg atggtttgac taccatacag gggaagacat aaaggtcaga
     5041 ggaaaacttc aaacattttc agctcctttt gatacaataa atctacatgt ccggggaggt
     5101 tatattctcc catgtcaaga accggcacag aacacatatt acagtcgaca gaaatacatg
     5161 aaactcatcg tagctgcaga tgacaaccaa acagcaaaag gaactctatt ctgggatgat
     5221 ggagagtcga tagacacata tgaaaaaaat caatatacct tgatagaatt caatttaaat
     5281 cagaaaaatt taacaagtac tgtattgaaa gatggatata gaaacaaaag tgagatgaaa
     5341 cttggaagta tctatgtatg gggaaaagga acaaccagta tcagtcaggt taatctgact
     5401 tatggtggga atgaacaaca gctttcattt actcaagacg aagctaagga gatactaaca
     5461 attgacctga aaaatgtaac tgttactctt gatgaaccaa tacaaatcag ctggtcatga
     5521 agatcaccat cagtttgaat tcctgaggag gaattgatat ccggtccacg gttctacttt
     5581 cctgactccc tattttcttt aaaacagaat tttcacactc cttatctaat atttctccat
     5641 tgtatgcatc aaaagaactg ctaactagtt ctatgaatgc aacctggatg tcacctgctc
     5701 agcaggctta acgggatttg aataattata atactttcca agcaacagct aaacctggaa
     5761 acttgtttgc atgtaactag gataagacta aagaaagtaa tttttaaata aacacaattt
     5821 ttgtaagtat aaagtataca taataataca atcaattgtg tagaccaaac ttatttaaca
     5881 aatcacaaca tttaataatt ttgtactatg tatacgtcat tacgaaacag aaaataaaag
     5941 ttgtataaat aca
//
