LOCUS       NM_178848               1369 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus sirtuin 5 (Sirt5), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_178848
VERSION     NM_178848.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Zhang,X., Ling,C., Xiong,Z., Gong,T., Luo,S., Liu,X., Zhang,L.,
            Liao,C., Lu,Y., Huang,X., Zhou,W., Zhou,S., Liu,Y. and Tang,J.
  TITLE     Desuccinylation of TBK1 by SIRT5 regulates inflammatory response of
            macrophages in sepsis
  JOURNAL   Cell Rep 43 (12), 115060 (2024)
   PUBMED   39673708
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Zhang,Y., Zhang,B.B., Bharathi,S.S., Bons,J., Rose,J.P., Shah,S.,
            Dobrowolski,S.F., Sims-Lucas,S., Schilling,B. and Goetzman,E.S.
  TITLE     Sirtuin-5 Is Recruited to Hepatic Peroxisomes in Mice Fed
            Dodecanedioic Acid but Has Little Impact on the Peroxisomal
            Succinylome
  JOURNAL   Biomolecules 14 (12), 1508 (2024)
   PUBMED   39766215
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1369)
  AUTHORS   Yokoyama,T., Takayama,Y., Mizuguchi,M., Nabeshima,Y. and Kusaka,K.
  TITLE     SIRT5 mutants reveal the role of conserved asparagine and glutamine
            residues in the NAD+-binding pocket
  JOURNAL   FEBS Lett 598 (18), 2269-2280 (2024)
   PUBMED   39031546
  REMARK    GeneRIF: SIRT5 mutants reveal the role of conserved asparagine and
            glutamine residues in the NAD[+]-binding pocket.
REFERENCE   4  (bases 1 to 1369)
  AUTHORS   Li,J., Yao,Y., Lei,X., Bao,J., An,S., Hu,H., Sha,T., Huang,Q.,
            Li,T., Zeng,Z., Wang,X. and Cai,S.
  TITLE     SIRTUIN 5 ALLEVIATES EXCESSIVE MITOCHONDRIAL FISSION VIA
            DESUCCINYLATION OF ATPASE INHIBITORY FACTOR 1 IN SEPSIS-INDUCED
            ACUTE KIDNEY INJURY
  JOURNAL   Shock 62 (2), 235-244 (2024)
   PUBMED   38754030
  REMARK    GeneRIF: SIRTUIN 5 ALLEVIATES EXCESSIVE MITOCHONDRIAL FISSION VIA
            DESUCCINYLATION OF ATPASE INHIBITORY FACTOR 1 IN SEPSIS-INDUCED
            ACUTE KIDNEY INJURY.
REFERENCE   5  (bases 1 to 1369)
  AUTHORS   Yuan,T., Kumar,S., Skinner,M.E., Victor-Joseph,R., Abuaita,M.,
            Keijer,J., Zhang,J., Kunkel,T.J., Liu,Y., Petrunak,E.M.,
            Saunders,T.L., Lieberman,A.P., Stuckey,J.A., Neamati,N.,
            Al-Murshedi,F., Alfadhel,M., Spelbrink,J.N., Rodenburg,R., de
            Boer,V.C.J. and Lombard,D.B.
  TITLE     Human SIRT5 variants with reduced stability and activity do not
            cause neuropathology in mice
  JOURNAL   iScience 27 (6), 109991 (2024)
   PUBMED   38846003
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1369)
  AUTHORS   Witt,O., Deubzer,H.E., Milde,T. and Oehme,I.
  TITLE     HDAC family: What are the cancer relevant targets?
  JOURNAL   Cancer Lett 277 (1), 8-21 (2009)
   PUBMED   18824292
  REMARK    Review article
REFERENCE   7  (bases 1 to 1369)
  AUTHORS   Nakagawa,T., Lomb,D.J., Haigis,M.C. and Guarente,L.
  TITLE     SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates
            the urea cycle
  JOURNAL   Cell 137 (3), 560-570 (2009)
   PUBMED   19410549
  REMARK    GeneRIF: SIRT5 plays a pivotal role in ammonia detoxification and
            disposal by activating carbamoyl phosphate synthetase 1
REFERENCE   8  (bases 1 to 1369)
  AUTHORS   Schlicker,C., Gertz,M., Papatheodorou,P., Kachholz,B., Becker,C.F.
            and Steegborn,C.
  TITLE     Substrates and regulation mechanisms for the human mitochondrial
            sirtuins Sirt3 and Sirt5
  JOURNAL   J Mol Biol 382 (3), 790-801 (2008)
   PUBMED   18680753
REFERENCE   9  (bases 1 to 1369)
  AUTHORS   Nakamura,Y., Ogura,M., Tanaka,D. and Inagaki,N.
  TITLE     Localization of mouse mitochondrial SIRT proteins: shift of SIRT3
            to nucleus by co-expression with SIRT5
  JOURNAL   Biochem Biophys Res Commun 366 (1), 174-179 (2008)
   PUBMED   18054327
  REMARK    GeneRIF: results suggest that the SIRT3, SIRT4, and SIRT5 proteins
            exert distinct functions in mitochondria.
REFERENCE   10 (bases 1 to 1369)
  AUTHORS   Lombard,D.B., Alt,F.W., Cheng,H.L., Bunkenborg,J., Streeper,R.S.,
            Mostoslavsky,R., Kim,J., Yancopoulos,G., Valenzuela,D., Murphy,A.,
            Yang,Y., Chen,Y., Hirschey,M.D., Bronson,R.T., Haigis,M.,
            Guarente,L.P., Farese,R.V. Jr., Weissman,S., Verdin,E. and
            Schwer,B.
  TITLE     Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine
            acetylation
  JOURNAL   Mol Cell Biol 27 (24), 8807-8814 (2007)
   PUBMED   17923681
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC132121.3.
            
            On Nov 17, 2006 this sequence version replaced NM_178848.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031770.1, AK002609.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC132121.3         80292-80301
            11-160              AC132121.3         85476-85625
            161-294             AC132121.3         86589-86722
            295-520             AC132121.3         91452-91677
            521-608             AC132121.3         94430-94517
            609-662             AC132121.3         95257-95310
            663-786             AC132121.3         97866-97989
            787-902             AC132121.3         100255-100370
            903-1369            AC132121.3         109494-109960
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 21.6 cM"
     gene            1..1369
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /note="sirtuin 5"
                     /db_xref="GeneID:68346"
                     /db_xref="MGI:MGI:1915596"
     exon            1..10
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            11..160
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..978
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /note="NAD-dependent deacetylase sirtuin-5; NAD-dependent
                     lysine demalonylase and desuccinylase sirtuin-5,
                     mitochondrial"
                     /codon_start=1
                     /product="NAD-dependent protein deacylase sirtuin-5,
                     mitochondrial"
                     /protein_id="NP_849179.1"
                     /db_xref="CCDS:CCDS26478.1"
                     /db_xref="GeneID:68346"
                     /db_xref="MGI:MGI:1915596"
                     /translation="MRPLLIAPGRFISQLCCRRKPPASPQSKICLTMARPSSNMADFR
                     KCFANAKHIAIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQAFARNPSQVWEFY
                     HYRREVMRSKEPNPGHLAIAQCEARLRDQGRRVVVITQNIDELHRKAGTKNLLEIHGT
                     LFKTRCTSCGTVAENYRSPICPALAGKGAPEPETQDARIPVDKLPRCEEAGCGGLLRP
                     HVVWFGENLDPAILEEVDRELALCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNM
                     ETTPATDRFRFHFPGPCGKTLPEALAPHETERTS"
     exon            161..294
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            295..520
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            521..608
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            609..662
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            663..786
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            787..902
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1369
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1343..1348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1369
                     /gene="Sirt5"
                     /gene_synonym="0610012J09Rik; 1500032M05Rik"
                     /note="major polyA site"
ORIGIN      
        1 ctttttgcag cctgcctggg gaagtaagac ttcaacgaaa acctgatgcg acctctcctg
       61 attgctccgg gccgattcat ttcccagttg tgttgtagac gaaagcctcc tgcctcccca
      121 caaagcaaga tctgcctcac catggctcgt ccaagttcaa atatggcaga ctttcggaag
      181 tgttttgcga acgccaagca catagccatc atctcggggg ctggcgttag tgcggagagt
      241 ggggttccca ctttcagagg cgctggaggt tactggagaa aatggcaggc tcaggacctg
      301 gcaacccctc aggcctttgc tcgaaaccca tcacaggtgt gggagtttta ccactaccgg
      361 agggaggtca tgcggagcaa agaacccaac cccgggcacc tggccattgc ccagtgtgaa
      421 gcccggcttc gtgaccaggg cagacgggtt gtggtcatca cccagaacat tgacgagttg
      481 catcgcaagg ctggcaccaa gaaccttctg gaaatccacg gaaccttatt taaaactcgg
      541 tgtacctcgt gtggcactgt tgccgagaac tataggagtc cgatctgccc agctttagca
      601 ggaaaagggg ccccagagcc agagactcaa gacgccagaa tcccagtcga caaacttccc
      661 cggtgcgagg aggcaggatg cggaggcttg ctgcgacctc acgtggtgtg gtttggagaa
      721 aacctggatc ctgccattct ggaggaggtg gacagagagc tcgccctctg tgacctgtgt
      781 ctagtggtgg gaacatcctc tgtggtctac ccggctgcca tgtttgcccc tcaggtggct
      841 tccaggggag tcccggtggc cgagtttaac atggagacca ccccagccac cgacagattc
      901 aggtttcatt ttcccggacc ctgtgggaaa actcttcctg aagcccttgc tcctcatgaa
      961 actgaaagga cttcttaacc gccctgtgga aagaggagaa ggacctgcag tacggtatcc
     1021 tggagtgcta aagcagggca ctaacgggaa aaatggcttt atggatggtg agctgaactc
     1081 tggaaaaata tggaaacacc cttcaagccc caagcagaca atctgttacg tgatgggttt
     1141 caaaatacca gcagcaaatg tgtttgatct ggaagaggct gtcaagtcct tccatattat
     1201 ttgatttgaa ctgaaatatg agtaattggg atttgatatt ttttggttag ttactggaag
     1261 ggaaaatttt gtaattaaat tgctttagaa ggcaattatc ctgattgtat gtttgcatct
     1321 tgggcaaaaa cagaaaaaga gaattaaaac cctgaaagtt aaaccctca
//
