LOCUS       NM_133760               1553 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sirtuin 4 (Sirt4), transcript variant 2, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_133760 XM_485674 XM_988059
VERSION     NM_133760.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Yang,G., Xiang,J., Yang,X., Liu,X., Li,Y., Li,L., Kang,L., Liang,Z.
            and Yang,S.
  TITLE     Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to
            modulate renal fibrosis through alternative splicing-mediated
            upregulation of CCN2
  JOURNAL   Elife 13, 98524 (2024)
   PUBMED   39495216
  REMARK    GeneRIF: Nuclear translocation of SIRT4 mediates deacetylation of
            U2AF2 to modulate renal fibrosis through alternative
            splicing-mediated upregulation of CCN2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Xing,D., Zhang,W., Cui,W., Yao,X., Xiao,Y., Chen,L., Yuan,S.,
            Duan,Y., Yu,W., Pan,P. and Lu,Y.
  TITLE     SIRT4 promotes neuronal apoptosis in models of Alzheimer's disease
            via the STAT2-SIRT4-mTOR pathway
  JOURNAL   Am J Physiol Cell Physiol 326 (6), C1697-C1709 (2024)
   PUBMED   38586875
  REMARK    GeneRIF: SIRT4 promotes neuronal apoptosis in models of Alzheimer's
            disease via the STAT2-SIRT4-mTOR pathway.
REFERENCE   3  (bases 1 to 1553)
  AUTHORS   Tucker,S.A., Hu,S.H., Vyas,S., Park,A., Joshi,S., Inal,A., Lam,T.,
            Tan,E., Haigis,K.M. and Haigis,M.C.
  TITLE     SIRT4 loss reprograms intestinal nucleotide metabolism to support
            proliferation following perturbation of homeostasis
  JOURNAL   Cell Rep 43 (4), 113975 (2024)
   PUBMED   38507411
  REMARK    GeneRIF: SIRT4 loss reprograms intestinal nucleotide metabolism to
            support proliferation following perturbation of homeostasis.
REFERENCE   4  (bases 1 to 1553)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 1553)
  AUTHORS   Lin,S., Wu,B., Hu,X. and Lu,H.
  TITLE     Sirtuin 4 (Sirt4) downregulation contributes to chondrocyte
            senescence and osteoarthritis via mediating mitochondrial
            dysfunction
  JOURNAL   Int J Biol Sci 20 (4), 1256-1278 (2024)
   PUBMED   38385071
  REMARK    GeneRIF: Sirtuin 4 (Sirt4) downregulation contributes to
            chondrocyte senescence and osteoarthritis via mediating
            mitochondrial dysfunction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1553)
  AUTHORS   Witt,O., Deubzer,H.E., Milde,T. and Oehme,I.
  TITLE     HDAC family: What are the cancer relevant targets?
  JOURNAL   Cancer Lett 277 (1), 8-21 (2009)
   PUBMED   18824292
  REMARK    Review article
REFERENCE   7  (bases 1 to 1553)
  AUTHORS   Nakamura,Y., Ogura,M., Tanaka,D. and Inagaki,N.
  TITLE     Localization of mouse mitochondrial SIRT proteins: shift of SIRT3
            to nucleus by co-expression with SIRT5
  JOURNAL   Biochem Biophys Res Commun 366 (1), 174-179 (2008)
   PUBMED   18054327
REFERENCE   8  (bases 1 to 1553)
  AUTHORS   Lombard,D.B., Alt,F.W., Cheng,H.L., Bunkenborg,J., Streeper,R.S.,
            Mostoslavsky,R., Kim,J., Yancopoulos,G., Valenzuela,D., Murphy,A.,
            Yang,Y., Chen,Y., Hirschey,M.D., Bronson,R.T., Haigis,M.,
            Guarente,L.P., Farese,R.V. Jr., Weissman,S., Verdin,E. and
            Schwer,B.
  TITLE     Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine
            acetylation
  JOURNAL   Mol Cell Biol 27 (24), 8807-8814 (2007)
   PUBMED   17923681
REFERENCE   9  (bases 1 to 1553)
  AUTHORS   Haigis,M.C., Mostoslavsky,R., Haigis,K.M., Fahie,K.,
            Christodoulou,D.C., Murphy,A.J., Valenzuela,D.M., Yancopoulos,G.D.,
            Karow,M., Blander,G., Wolberger,C., Prolla,T.A., Weindruch,R.,
            Alt,F.W. and Guarente,L.
  TITLE     SIRT4 inhibits glutamate dehydrogenase and opposes the effects of
            calorie restriction in pancreatic beta cells
  JOURNAL   Cell 126 (5), 941-954 (2006)
   PUBMED   16959573
REFERENCE   10 (bases 1 to 1553)
  AUTHORS   Argmann,C. and Auwerx,J.
  TITLE     Insulin secretion: SIRT4 gets in on the act
  JOURNAL   Cell 126 (5), 837-839 (2006)
   PUBMED   16959562
  REMARK    GeneRIF: controls insulin secretion in pancreatic beta cells; also
            transfers an ADP-ribosyl group from NAD to histones
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159539.6 and AC117735.8.
            
            On or before Nov 17, 2009 this sequence version replaced
            XM_988059.1, XM_485674.5.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022653.1, SRR1660813.256274.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC159539.6         48044-48196         c
            154-648             AC159539.6         46336-46830         c
            649-929             AC159539.6         43957-44237         c
            930-943             AC117735.8         1-14
            944-1154            AC117735.8         433-643
            1155-1553           AC117735.8         1837-2235
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 56.1 cM"
     gene            1..1553
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /note="sirtuin 4"
                     /db_xref="GeneID:75387"
                     /db_xref="MGI:MGI:1922637"
     exon            1..153
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            154..648
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1162
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /EC_number="2.3.1.286"
                     /note="NAD-dependent protein biotinylase sirtuin-4"
                     /codon_start=1
                     /product="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial"
                     /protein_id="NP_598521.1"
                     /db_xref="CCDS:CCDS51628.1"
                     /db_xref="GeneID:75387"
                     /db_xref="MGI:MGI:1922637"
                     /translation="MSGLTFRPTKGRWITHLSRPRSCGPSGLFVPPSPPLDPEKIKEL
                     QRFISLSKKLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHIDFVRSAPVRQRY
                     WARNFVGWPQFSSHQPNPAHWALSNWERLGKLHWLVTQNVDALHSKAGSQRLTELHGC
                     MHRVLCLNCGEQTARRVLQERFQALNPSWSAEAQGVAPDGDVFLTEEQVRSFQVPCCD
                     RCGGPLKPDVVFFGDTVNPDKVDFVHRRVKEADSLLVVGSSLQVYSGYRFILTAREQK
                     LPIAILNIGPTRSDDLACLKLDSRCGELLPLIDPRRQHSDVQRLEMNFPLSSAAQDP"
     exon            649..943
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1154
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1553
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1530..1535
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1553
                     /gene="Sirt4"
                     /gene_synonym="4930596O17Rik"
                     /note="major polyA site"
ORIGIN      
        1 atccgtagag gagcgcccaa gaatcttggc gtgggagaca cgtaggattc tgggacttgt
       61 agttttcttc caactagatc ggcttcgcgg actacatttc ccaagatgca cacgtaacaa
      121 gcgagtccca ctgttgggtg agaattgtgg aagaataaga atgagcggat tgactttcag
      181 gccgacaaag ggccgttgga tcacccacct cagccggccg cgttcttgtg gaccctcggg
      241 gttatttgtg ccgcccagcc ctcctttgga ccctgaaaag atcaaagagt tacagcgctt
      301 cattagcctt tccaagaaac tcctcgtgat gacaggcgcg gggatctcca ccgagtccgg
      361 catcccagac tacaggtcag aaaaggtggg actttacgcc cgcactgacc ggagacccat
      421 ccagcacatt gatttcgtcc gcagtgctcc ggtccgccag cggtactggg cccgaaactt
      481 tgtgggctgg cctcaattct cctctcacca acccaaccca gcacactggg ctctgagcaa
      541 ctgggagaga ctggggaagc tgcactggtt ggtgactcag aacgtggacg ctttgcactc
      601 caaagcaggg agtcagcggc tgacggagct ccacggatgc atgcacagag tcctgtgcct
      661 gaactgtggg gagcagactg cccgcagggt gctgcaggaa cgcttccaag ccctgaaccc
      721 cagctggagc gccgaggcgc agggcgtggc tcccgacggc gacgtgttcc tcactgagga
      781 gcaggtccgg agctttcagg tcccgtgctg tgatcgatgc ggcggccctc tgaaaccgga
      841 cgtcgttttc tttggggaca cggtgaaccc agacaaggtt gactttgtgc accggcgtgt
      901 gaaagaggcg gactccctac tggtggtggg atcatccctg caggtgtact ctggttacag
      961 gttcatcctc accgcccgcg agcaaaagct cccaatagcc attctgaata tcggccccac
     1021 ccggtctgac gatttggctt gcctgaagct ggattcccgc tgtggagagt tgctgccttt
     1081 aatagacccg cggagacagc actctgatgt ccaaaggctg gaaatgaact ttcctctgag
     1141 ttccgctgct caagatccct aaggatcccc ccacccccca atgccgctcc aactctgaat
     1201 cctagaggtg gcaaagccac gtctagaggc caggcaccct cctgtttaca gaaatgagag
     1261 ctgcccccta aggaaagact tttccaaaga aatagctgct cttgatggag tagctgtgtc
     1321 gccttctggt aaccggaaga aggaccaggc agaagtcagt aaaccacaac tgtctatgag
     1381 gggcaacgtg gagatagatc tcactcattc agggcattta taattcattc ataattccaa
     1441 atggatgtgt ctgactgaag cactttgata tttctgatgg caggagcaat agctaacacg
     1501 gctttttttt ttttaattat tagccaaata ataaattatt tttaatagca taa
//
