LOCUS       NM_022433               1484 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sirtuin 3 (Sirt3), transcript variant 1, mRNA.
ACCESSION   NM_022433
VERSION     NM_022433.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Hu,Y., Zheng,Y., Liu,C., You,Y., Wu,Y., Wang,P., Wu,Y., Ba,H.,
            Lu,J., Yuan,Y., Liu,P. and Mao,Y.
  TITLE     Mitochondrial MOF regulates energy metabolism in heart failure via
            ATP5B hyperacetylation
  JOURNAL   Cell Rep 43 (10), 114839 (2024)
   PUBMED   39392752
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Chen,J.X., Zhang,J., Chen,Y. and Zeng,H.
  TITLE     Knockout of Sirtuin 3 in endothelial cells impairs
            endothelial-dependent relaxation and myogenic response in mice
  JOURNAL   Physiol Rep 12 (20), e70060 (2024)
   PUBMED   39425510
  REMARK    GeneRIF: Knockout of Sirtuin 3 in endothelial cells impairs
            endothelial-dependent relaxation and myogenic response in mice.
REFERENCE   3  (bases 1 to 1484)
  AUTHORS   Richardson,K.K., Adam,G.O., Ling,W., Warren,A.,
            Marques-Carvalho,A., Thostenson,J.D., Krager,K., Aykin-Burns,N.,
            Byrum,S.D., Almeida,M. and Kim,H.N.
  TITLE     Mitochondrial protein deacetylation by SIRT3 in osteoclasts
            promotes bone resorption with aging in female mice
  JOURNAL   Mol Metab 88, 102012 (2024)
   PUBMED   39154858
  REMARK    GeneRIF: Mitochondrial protein deacetylation by SIRT3 in
            osteoclasts promotes bone resorption with aging in female mice.
REFERENCE   4  (bases 1 to 1484)
  AUTHORS   Li,Y., Kong,E., Ding,R., Chu,R., Lu,J., Deng,M., Hua,T., Yang,M.,
            Wang,H., Chen,D., Song,H., Wei,H., Zhang,P., Han,C. and Yuan,H.
  TITLE     Hyperglycemia-induced Sirt3 downregulation increases microglial
            aerobic glycolysis and inflammation in diabetic neuropathic pain
            pathogenesis
  JOURNAL   CNS Neurosci Ther 30 (8), e14913 (2024)
   PUBMED   39123294
  REMARK    GeneRIF: Hyperglycemia-induced Sirt3 downregulation increases
            microglial aerobic glycolysis and inflammation in diabetic
            neuropathic pain pathogenesis.
REFERENCE   5  (bases 1 to 1484)
  AUTHORS   Ge,Y., Wu,X., Cai,Y., Hu,Q., Wang,J., Zhang,S., Zhao,B., Cui,W.,
            Wu,Y., Wang,Q., Feng,T., Liu,H., Qu,Y. and Ge,S.
  TITLE     FNDC5 prevents oxidative stress and neuronal apoptosis after
            traumatic brain injury through SIRT3-dependent regulation of
            mitochondrial quality control
  JOURNAL   Cell Death Dis 15 (5), 364 (2024)
   PUBMED   38802337
  REMARK    GeneRIF: FNDC5 prevents oxidative stress and neuronal apoptosis
            after traumatic brain injury through SIRT3-dependent regulation of
            mitochondrial quality control.
            Erratum:[Cell Death Dis. 2024 Jul 24;15(7):527. doi:
            10.1038/s41419-024-06901-5.. PMID: 39048543]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1484)
  AUTHORS   Lombard,D.B., Alt,F.W., Cheng,H.L., Bunkenborg,J., Streeper,R.S.,
            Mostoslavsky,R., Kim,J., Yancopoulos,G., Valenzuela,D., Murphy,A.,
            Yang,Y., Chen,Y., Hirschey,M.D., Bronson,R.T., Haigis,M.,
            Guarente,L.P., Farese,R.V. Jr., Weissman,S., Verdin,E. and
            Schwer,B.
  TITLE     Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine
            acetylation
  JOURNAL   Mol Cell Biol 27 (24), 8807-8814 (2007)
   PUBMED   17923681
  REMARK    GeneRIF: These results extend the recent finding of lysine
            acetylation of mitochondrial proteins and demonstrate that SIRT3
            has evolved to control reversible lysine acetylation in this
            organelle.
REFERENCE   7  (bases 1 to 1484)
  AUTHORS   Hallows,W.C., Lee,S. and Denu,J.M.
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases
  JOURNAL   Proc Natl Acad Sci U S A 103 (27), 10230-10235 (2006)
   PUBMED   16790548
REFERENCE   8  (bases 1 to 1484)
  AUTHORS   Shi,T., Wang,F., Stieren,E. and Tong,Q.
  TITLE     SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial
            function and thermogenesis in brown adipocytes
  JOURNAL   J Biol Chem 280 (14), 13560-13567 (2005)
   PUBMED   15653680
  REMARK    GeneRIF: Results demonstrate that SIRT3 activates mitochondria and
            plays an important role in adaptive thermogenesis in brown adipose
            tissue.
            GeneRIF: Caloric restriction and cold exposure elevate SIRT3
            expression in brown adipose to regulate thermogenesis through
            PGC-1alpha and UCP1.
REFERENCE   9  (bases 1 to 1484)
  AUTHORS   Yang,Y.H., Chen,Y.H., Zhang,C.Y., Nimmakayalu,M.A., Ward,D.C. and
            Weissman,S.
  TITLE     Cloning and characterization of two mouse genes with homology to
            the yeast Sir2 gene
  JOURNAL   Genomics 69 (3), 355-369 (2000)
   PUBMED   11056054
REFERENCE   10 (bases 1 to 1484)
  AUTHORS   Fukasawa,K.M., Li,W.H., Yagi,K., Luo,C.C. and Li,S.S.
  TITLE     Molecular evolution of mammalian lactate dehydrogenase-A genes and
            pseudogenes: association of a mouse processed pseudogene with a B1
            repetitive sequence
  JOURNAL   Mol Biol Evol 3 (4), 330-342 (1986)
   PUBMED   3444409
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF299338.1, CT010402.1 and BF681854.1.
            
            On May 14, 2008 this sequence version replaced NM_022433.1.
            
            Transcript Variant: This variant (1) has an alternate 5' exon and
            an additional segment, resulting in a downstream AUG start codon,
            as compared to variant 3. The resulting isoform (1) is shorter at
            the N-terminus, as compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF299338.1, SRR5189676.106381.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-708               AF299338.1         1-708
            709-1067            CT010402.1         415-773
            1068-1461           AF299338.1         1068-1461
            1462-1484           BF681854.1         642-664
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 86.07 cM"
     gene            1..1484
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /note="sirtuin 3"
                     /db_xref="GeneID:64384"
                     /db_xref="MGI:MGI:1927665"
     exon            1..152
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            153..341
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..1068
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /EC_number="2.3.1.286"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NAD-dependent deacetylase sirtuin-3; SIRT3L mitochondrial;
                     mitochondrial protein lysine deacetylase; mSIR2L3;
                     SIR2-like protein 3; silent mating type information
                     regulation 2, (S.cerevisiae, homolog)-like 3; regulatory
                     protein SIR2 homolog 3; NAD-dependent protein deacetylase
                     sirtuin-3; NAD-dependent protein delactylase sirtuin-3"
                     /codon_start=1
                     /product="NAD-dependent protein deacetylase sirtuin-3
                     isoform 1"
                     /protein_id="NP_071878.2"
                     /db_xref="CCDS:CCDS21989.1"
                     /db_xref="GeneID:64384"
                     /db_xref="MGI:MGI:1927665"
                     /translation="MVGAGISTPSGIPDFRSPGSGLYSNLQQYDIPYPEAIFELGFFF
                     HNPKPFFMLAKELYPGHYRPNVTHYFLRLLHDKELLLRLYTQNIDGLERASGIPASKL
                     VEAHGTFVTATCTVCRRSFPGEDIWADVMADRVPRCPVCTGVVKPDIVFFGEQLPARF
                     LLHMADFALADLLLILGTSLEVEPFASLSEAVQKSVPRLLINRDLVGPFVLSPRRKDV
                     VQLGDVVHGVERLVDLLGWTQELLDLMQRERGKLDGQDR"
     exon            342..574
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            575..675
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            676..837
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1047
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1468
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1443..1448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /note="hexamer: AATAAA"
     polyA_site      1461
                     /gene="Sirt3"
                     /gene_synonym="2310003L23Rik; Sir2l3"
                     /note="major polyA site"
ORIGIN      
        1 acttccgcta aacttctccc gggtttctgg ccctgccctt gaggcattaa agagtagagg
       61 tgcctgggga cagctagcca ctgactggtc acgtagcctc aagcctgcag acttgggtcc
      121 tctgaaaccg gatggcgttt ggcgaggact agtgttacag gtgggagaag gcccatatcc
      181 ctctgtgtgg gagcctcagg cggctttgga ggtggaggaa gcagtgagaa gaagttttct
      241 ctgcaggatg tagctgagct gcttcggacc agagcctgca gtagggtggt ggtcatggtg
      301 ggggccggca tcagcacacc cagtggcatc ccggacttca gatccccagg gagcggcctc
      361 tacagcaacc ttcagcagta tgacatcccg taccctgaag ccatctttga acttggcttt
      421 ttctttcaca accccaagcc ctttttcatg ttggccaagg agctgtaccc tgggcactac
      481 aggcccaatg tcactcacta cttcctgagg ctcctccacg acaaggagct gcttctgcgg
      541 ctctatacac agaacatcga cgggcttgag agagcatctg ggatccctgc ctcaaagctg
      601 gttgaagccc acgggacctt tgtaacagct acatgcacgg tctgtcgaag gtccttccca
      661 ggggaagaca tatgggctga tgtgatggcg gacagggtgc cccgctgccc tgtctgtact
      721 ggcgttgtga aacccgacat tgtgttcttt ggggagcagc tgcctgcaag gttcctactc
      781 catatggctg acttcgcttt ggcagatctg ctactcattc ttgggacctc cctggaggtg
      841 gagccttttg ccagcttgtc tgaagcagta cagaaatcag tgccccgact gctcatcaat
      901 cgagacttgg tggggccgtt cgttctgagt cctcgaagga aagatgtggt ccagctaggg
      961 gatgtagttc atggtgtgga aaggctggtg gacctcctgg ggtggacaca agaactgctg
     1021 gatcttatgc agcgggaacg tggcaagctg gatggacagg acagataaga ctatggcttc
     1081 ttcacctggg gaagtcacac agcagatcat cctatgtcca gcaagacttc atgcctgaag
     1141 acagctccaa cacgtttaca aacatgaacc agaccacaac atgtggcctg gacagtggtc
     1201 ctccgaggct gcctttggaa aggctgacca gggatgtcta cccttggggc ccctccatgt
     1261 gtgcgccctg tccacctcat cactgctgaa ggtgtagtgc aggtgctgct ttctgcagcg
     1321 gcccttaagt tatcacgagg gcagcacagc acgcccgtcg ccaggcaggc gatgcactag
     1381 ggcaatctag catgttgatc ggtaaagtgg catctttaac tacaacatca tttcttgcat
     1441 gaaataaact tagtataaaa acttggcaaa aaaaaaaaaa aaaa
//
