LOCUS       NM_001164481            3847 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus signal-induced proliferation associated gene 1
            (Sipa1), transcript variant 1, mRNA.
ACCESSION   NM_001164481
VERSION     NM_001164481.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Horitani,S., Ueda,Y., Kamioka,Y., Kondo,N., Ikeda,Y., Naganuma,M.
            and Kinashi,T.
  TITLE     The critical role of Rap1-GAPs Rasa3 and Sipa1 in T cells for
            pulmonary transit and egress from the lymph nodes
  JOURNAL   Front Immunol 14, 1234747 (2023)
   PUBMED   37545505
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3847)
  AUTHORS   Sauter,R.J., Sauter,M., Reis,E.S., Emschermann,F.N., Nording,H.,
            Ebenhoch,S., Kraft,P., Munzer,P., Mauler,M., Rheinlaender,J.,
            Madlung,J., Edlich,F., Schaffer,T.E., Meuth,S.G., Duerschmied,D.,
            Geisler,T., Borst,O., Gawaz,M., Kleinschnitz,C., Lambris,J.D. and
            Langer,H.F.
  TITLE     Functional Relevance of the Anaphylatoxin Receptor C3aR for
            Platelet Function and Arterial Thrombus Formation Marks an
            Intersection Point Between Innate Immunity and Thrombosis
  JOURNAL   Circulation 138 (16), 1720-1735 (2018)
   PUBMED   29802205
  REMARK    Erratum:[Circulation. 2019 Jan 15;139(3):e8. doi:
            10.1161/CIR.0000000000000650.. Durschmied, Daniel [corrected to
            Duerschmied, Daniel]. PMID: 30640538]
REFERENCE   4  (bases 1 to 3847)
  AUTHORS   Xiao,P., Dolinska,M., Sandhow,L., Kondo,M., Johansson,A.S.,
            Bouderlique,T., Zhao,Y., Li,X., Dimitriou,M., Rassidakis,G.Z.,
            Hellstrom-Lindberg,E., Minato,N., Walfridsson,J., Scadden,D.T.,
            Sigvardsson,M. and Qian,H.
  TITLE     Sipa1 deficiency-induced bone marrow niche alterations lead to the
            initiation of myeloproliferative neoplasm
  JOURNAL   Blood Adv 2 (5), 534-548 (2018)
   PUBMED   29514790
  REMARK    GeneRIF: Sipa1 loss-induced BM niche alterations likely enable
            evolution of clonal hematopoiesis to the hematological
            malignancies.
REFERENCE   5  (bases 1 to 3847)
  AUTHORS   Xu,Y., Ikeda,S., Sumida,K., Yamamoto,R., Tanaka,H. and Minato,N.
  TITLE     Sipa1 deficiency unleashes a host-immune mechanism eradicating
            chronic myelogenous leukemia-initiating cells
  JOURNAL   Nat Commun 9 (1), 914 (2018)
   PUBMED   29500416
  REMARK    GeneRIF: Sipa1 deficiency uncovers a host immune mechanism
            potentially capable of eradicating Bcr-Abl(+) hematopoietic
            progenitor cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3847)
  AUTHORS   Ebrahimi,S., Wang,E., Udar,N., Arnold,E., Burbee,D., Small,K. and
            Sawicki,M.P.
  TITLE     Genomic organization and cloning of the human homologue of murine
            Sipa-1
  JOURNAL   Gene 214 (1-2), 215-221 (1998)
   PUBMED   9651531
REFERENCE   7  (bases 1 to 3847)
  AUTHORS   Kurachi,H., Wada,Y., Tsukamoto,N., Maeda,M., Kubota,H., Hattori,M.,
            Iwai,K. and Minato,N.
  TITLE     Human SPA-1 gene product selectively expressed in lymphoid tissues
            is a specific GTPase-activating protein for Rap1 and Rap2.
            Segregate expression profiles from a rap1GAP gene product
  JOURNAL   J Biol Chem 272 (44), 28081-28088 (1997)
   PUBMED   9346962
REFERENCE   8  (bases 1 to 3847)
  AUTHORS   Wada,Y., Kubota,H., Maeda,M., Taniwaki,M., Hattori,M., Imamura,S.,
            Iwai,K. and Minato,N.
  TITLE     Mitogen-inducible SIPA1 is mapped to the conserved syntenic groups
            of chromosome 19 in mouse and chromosome 11q13.3 centromeric to
            BCL1 in human
  JOURNAL   Genomics 39 (1), 66-73 (1997)
   PUBMED   9027487
REFERENCE   9  (bases 1 to 3847)
  AUTHORS   Minato,N.
  TITLE     [Regulatory mechanisms of lymphocyte proliferation: roles of Spa-1
            gene]
  JOURNAL   Hum Cell 9 (1), 11-16 (1996)
   PUBMED   9183624
  REMARK    Review article
REFERENCE   10 (bases 1 to 3847)
  AUTHORS   Hattori,M., Tsukamoto,N., Nur-e-Kamal,M.S., Rubinfeld,B., Iwai,K.,
            Kubota,H., Maruta,H. and Minato,N.
  TITLE     Molecular cloning of a novel mitogen-inducible nuclear protein with
            a Ran GTPase-activating domain that affects cell cycle progression
  JOURNAL   Mol Cell Biol 15 (1), 552-560 (1995)
   PUBMED   7799964
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134563.5.
            
            On Mar 25, 2022 this sequence version replaced NM_001164481.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156280.1, SRR7345562.1610280.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AC134563.5         117452-117537
            87-340              AC134563.5         118300-118553
            341-371             AC134563.5         119515-119545
            372-1169            AC134563.5         120030-120827
            1170-1297           AC134563.5         121418-121545
            1298-1474           AC134563.5         121631-121807
            1475-1649           AC134563.5         124813-124987
            1650-1753           AC134563.5         125350-125453
            1754-1911           AC134563.5         125726-125883
            1912-2521           AC134563.5         125985-126594
            2522-2796           AC134563.5         126868-127142
            2797-3015           AC134563.5         128268-128486
            3016-3127           AC134563.5         128575-128686
            3128-3236           AC134563.5         128772-128880
            3237-3338           AC134563.5         128995-129096
            3339-3393           AC134563.5         129181-129235
            3394-3472           AC134563.5         129427-129505
            3473-3847           AC134563.5         129579-129953
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 4.34 cM"
     gene            1..3847
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="signal-induced proliferation associated gene 1"
                     /db_xref="GeneID:20469"
                     /db_xref="MGI:MGI:107576"
     exon            1..86
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            87..340
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            341..371
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..1169
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     CDS             503..3619
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="GTPase-activating protein Spa-1; sipa-1"
                     /codon_start=1
                     /product="signal-induced proliferation-associated protein
                     1"
                     /protein_id="NP_001157953.1"
                     /db_xref="CCDS:CCDS29474.1"
                     /db_xref="GeneID:20469"
                     /db_xref="MGI:MGI:107576"
                     /translation="MWAGGVGSPRRGMAPAPTDDLFARKLRQPARPPLTPHTFEPRPA
                     RGPLLRSGSDAGEVRPPTPASPRARAHSHEDASRPAATPTRLFTDPLALLGLPAEEPE
                     PTFPPVLEPRWFAHYDVQSLLFDWAPRPRGTGSHIEANSGTLAEGQTTTSDLLLGAPG
                     FVSELGGEGELGLGGPISPPVPPALPNAAVSVLEEPQTRTTAYSLEHADLGAGYYRKY
                     FYGKEHQNFFGLDEALGPVAVSLRREEKEGSGGGTLHSYRVIVRTTQLRTLRGTISED
                     ALPPGPPRGLSPRKLLEHVAPRLSPTCLRLGSASPKVPRTLLTLDEQVLSFQRKVGIL
                     YCRAGQGSEEEMYNNQEAGAAFMQFLTLLGDVVRLKGFESYRAQLDTKTDSTGTHSLY
                     TTYQDHEIMFHVSTMLPYTPNNQQQLLRKRHIGNDIVTIVFQEPGSKPFCPTTIRSHF
                     QHVFLVVRAHAPCTPHTSYRVAVSRTQDTPAFGPALPEGGGPFAANADFRAFLLAKAL
                     NGEQAAGHARQFHAMATRTRQQYLQDLATNEVTTTSLDSASRFGLPSLGGRRRATPRS
                     PGAELQAAGALMWGVRAAPGARVAAGAETSGPEDAEVPCLLGISAETLVLVAPRDGRV
                     VFNCACRDVLAWTFSEHQLDLYHGRGEAITLRLDGAPGQAVGEVVARLQLVSRGCETR
                     ELALPRDGQGRLGFEVDAEGFITHVERFTFAETTGLRPGARLLRVCGQTLPKLGPEAA
                     AQMLRSAPKVCVTVLPPDESGRPRRSFSELYMLSLKEPSRRGGPEPVQDETGKLVILP
                     PTKQLLHFCLKDSSSPPGPGDLTEERTEFLRSHNSLSSGSSLSDEAPVLPNTTPDLLL
                     VTTANPSAPGTDRETPPSQDQSGSPSSHEDTSDSGPELRASILPRTLSLRNSISKIMS
                     EAGSETLEDEWQSISEIASTCNTILESLSREGQPISESGDPKEALKCDSEPEPGSLSE
                     KVSHLESMLWKLQEDLQREKADRAALEEEVRSLRHNNQRLLAESESAATRLLLASKHL
                     GAPTTDLA"
     exon            1170..1297
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1474
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1649
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1753
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1911
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2521
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2796
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..3015
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3127
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3128..3236
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3237..3338
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3339..3393
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3394..3472
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     exon            3473..3847
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3822..3827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="hexamer: AAGAAA"
     regulatory      3829..3834
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="hexamer: AACAAG"
     polyA_site      3844
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="major polyA site"
     polyA_site      3847
                     /gene="Sipa1"
                     /gene_synonym="Spa1"
                     /note="major polyA site"
ORIGIN      
        1 aggcggagcg gaggcgccgg gagcggtgtg agcaggcagc gggaccttgg tgcggaaggc
       61 agcggtggcc agcttgagcc cgagagtttc caaggagtct tcaaagaact catgaagtgc
      121 agcatcagag cttatcctag aagacagaca gacagacagg aagaccctga ggaggtctgc
      181 tcttgtttgg ttctggagac ccagctgagg ggcaccgtgg agctgctccc tgtcccctcc
      241 cagcctggcc cccttgatgc cactggatga tgcaaaaaaa agtactaatg gaggcctgcc
      301 cctgccccag ctgttggctc cattcctagc tcacgccgag cagctgcccc aggagctcct
      361 gtgtccttga ggcccatctg aacagccccc tcctcctgca gtgcaggaac cactgaagcc
      421 tcagccttct ggctgggcac caaggacccg tgcccaccaa tgcggcccgg cccccagaga
      481 gtcaggccca cagagcacgc ccatgtgggc cggcggtgtg gggagccctc ggcggggcat
      541 ggcccctgca cctaccgatg acctctttgc ccgtaagctt cgccaacctg cccggccccc
      601 actgacacca catacctttg agccgaggcc agctcggggc ccactcttgc gcagtggcag
      661 tgatgctggt gaagtccggc cccctacacc agccagcccc cgtgcccgtg cccacagcca
      721 cgaggatgcc agccgccctg ctgcaacccc tactcggctc ttcactgacc cactggcact
      781 gctagggttg ccagcagaag agccagagcc caccttcccg ccagtgctgg aaccccggtg
      841 gtttgctcac tatgatgtgc agagcttgct ctttgactgg gctccacgac ctcgggggac
      901 aggcagccat atagaggcaa actctgggac cttagctgag ggccagacta ccacctcaga
      961 tctactgctc ggggcacctg gctttgtgag cgagcttggt ggtgagggtg agctagggct
     1021 gggtgggcca atatccccac ctgtgccccc tgcactgcct aatgcggctg tgtccgtcct
     1081 ggaggagcca cagacccgga ccacagctta cagcctggag cacgcagatc tgggtgcagg
     1141 ctactaccgc aagtacttct atggcaaaga acaccagaac ttctttgggc tggatgaggc
     1201 gctgggtccg gtggccgtga gcctgcgacg ggaggagaaa gagggcagcg gagggggcac
     1261 cctacacagc taccgggtca tcgtgcggac cacgcagctc cggaccctcc gtggcaccat
     1321 ctcggaggac gcactgcctc ccggcccccc gaggggccta tctccgagga agcttctgga
     1381 acatgtggct ccacggctga gccccacctg cctgcgcctg ggttcagcct ctcccaaggt
     1441 gccccgcacg ctgcttactc tggatgagca agtgctgagc ttccaacgca aggtgggcat
     1501 cctgtactgc cgcgcaggcc agggctctga ggaagagatg tacaacaacc aggaggccgg
     1561 agcagccttt atgcagttcc ttaccttgct gggtgatgtg gtgcgactca aaggctttga
     1621 aagttaccgg gcccagctgg ataccaaaac ggattccacg ggcacacact cactctacac
     1681 cacctaccaa gaccatgaga tcatgtttca cgtgtccacg atgctgcctt acacgcctaa
     1741 taaccagcaa cagctcctga ggaagcgtca tatcggcaac gatattgtga ccatcgtgtt
     1801 ccaggagccc ggtagcaagc ccttctgccc tacgacaatc cgctctcact tccagcacgt
     1861 tttcttggtg gtgcgtgcgc atgctccctg caccccacac acctcataca gggtggcagt
     1921 gagccgcacc caggacactc ctgccttcgg tcctgcgctg ccagaaggcg gaggcccctt
     1981 tgcagccaat gccgatttcc gggcctttct gttggctaag gcactcaatg gtgagcaagc
     2041 ggctggtcat gcacgccagt tccacgccat ggctacacgc acacgccaac agtacctgca
     2101 ggacctggct actaatgaag tgaccactac ttcgctggac tcggcttcgc ggtttggcct
     2161 gccatctctg gggggtaggc gccgggcaac ccctcggagc ccaggcgcgg agctacaggc
     2221 ggcgggtgcg ctgatgtggg gcgtacgcgc ggctccaggg gcgcgggtcg cagcgggagc
     2281 tgaaacgagc ggtccggagg acgccgaggt gccctgcttg ttgggcatct cagcagagac
     2341 actggtgctg gtggcacctc gcgacggccg cgtggtcttc aattgtgcct gtcgcgacgt
     2401 attggcctgg accttctcag agcaccaact cgatctgtac cacgggcgcg gggaggcgat
     2461 cacgctgcgg ctcgacgggg ccccagggca agccgtgggc gaagtcgtgg cacgtctgca
     2521 gctggtgagc cgcgggtgtg agaccagaga actagcgctg cccagagatg gccaaggtcg
     2581 cctgggcttc gaggtggatg cagaaggctt catcacgcac gtggagcgct tcacgtttgc
     2641 ggagaccacg gggcttcggc ctggagctcg tttgctgcga gtctgcggcc agacgctgcc
     2701 caagctgggt cccgaagctg ctgcccagat gctgcgctct gcgcccaagg tctgcgtcac
     2761 ggtcctaccc ccagacgaga gcggccggcc gcgaaggagc ttttcggagc tctatatgct
     2821 ctctctgaag gaacccagcc ggcggggggg tccagagcca gtacaggatg aaactgggaa
     2881 gttggtcata ttgcctccca ccaagcagct gctacatttt tgcctgaaag acagcagcag
     2941 tcctccgggg cctggggatc tgactgagga gaggacagag ttcctgcgca gccacaactc
     3001 cctgtcatct ggaagctccc tgtccgatga ggctccagtc ctgcccaaca ccactccaga
     3061 cctcctcctt gtcaccactg ccaacccatc tgcacctggt actgacagag aaacaccccc
     3121 ttcccaggac cagtcaggaa gccccagtag ccatgaagac accagtgact caggcccaga
     3181 actgagggcc tccatcctgc ccagaacctt gtctctgcgg aattccatca gtaagattat
     3241 gtcggaagct ggcagtgaga ccctggagga tgagtggcag tccatctcag agatcgcctc
     3301 cacttgcaac acaattctgg agtcactgtc ccgggaggga caacccatct cagagagcgg
     3361 agaccccaag gaagctttaa agtgtgattc tgagccagaa cccgggagcc tgtcagaaaa
     3421 ggtctctcac ctagagtcca tgctctggaa gctccaggag gacctgcaga gggagaaggc
     3481 ggacagggca gccttggagg aggaggttcg gagcctcaga cacaacaacc agaggctgct
     3541 ggcagagtcc gagagtgccg ccacccgcct gctcctggcc tctaagcatc tgggtgcacc
     3601 cactactgac ctggcctgag ttccaatctg aatctggacc tgcttggaac tgcctggccc
     3661 ctcagagcaa ctgggtcata ctagtgccct tcctcaggac ttcttccctg cgctgaggcg
     3721 cgtcttagca ctgccccctc ttcccagccc atttggtggc taatgcctgt ccctgtttgt
     3781 aaatatcctg taaagaaaag gagacatcag agtttaaaaa aaagaaacaa caagaagaag
     3841 caaacaa
//
