LOCUS       NM_145581               2529 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sialic acid binding Ig-like lectin F (Siglecf),
            transcript variant 1, mRNA.
ACCESSION   NM_145581
VERSION     NM_145581.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Chetty,A., Darby,M.G., Pillaye,J., Taliep,A., Cunningham,A.F.,
            O'Shea,M.K., Katawa,G., Layland,L.E., Ritter,M. and Horsnell,W.G.C.
  TITLE     Induction of Siglec-FhiCD101hi eosinophils in the lungs following
            murine hookworm Nippostrongylus brasiliensis infection
  JOURNAL   Front Immunol 14, 1170807 (2023)
   PUBMED   37251384
  REMARK    GeneRIF: Induction of Siglec-F[hi]CD101[hi] eosinophils in the
            lungs following murine hookworm Nippostrongylus brasiliensis
            infection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wang,X., Liu,M., Zhang,J., Brown,N.K., Zhang,P., Zhang,Y., Liu,H.,
            Du,X., Wu,W., Devenport,M., Tao,W., Mao-Draayer,Y., Chen,G.Y.,
            Chen,Y.E., Zheng,P. and Liu,Y.
  TITLE     CD24-Siglec axis is an innate immune checkpoint against
            metaflammation and metabolic disorder
  JOURNAL   Cell Metab 34 (8), 1088-1103 (2022)
   PUBMED   35921817
REFERENCE   3  (bases 1 to 2529)
  AUTHORS   Ignacio,A., Shah,K., Bernier-Latmani,J., Koller,Y., Coakley,G.,
            Moyat,M., Hamelin,R., Armand,F., Wong,N.C., Ramay,H., Thomson,C.A.,
            Burkhard,R., Wang,H., Dufour,A., Geuking,M.B., McDonald,B.,
            Petrova,T.V., Harris,N.L. and McCoy,K.D.
  TITLE     Small intestinal resident eosinophils maintain gut homeostasis
            following microbial colonization
  JOURNAL   Immunity 55 (7), 1250-1267 (2022)
   PUBMED   35709757
REFERENCE   4  (bases 1 to 2529)
  AUTHORS   Ryu,S., Shin,J.W., Kwon,S., Lee,J., Kim,Y.C., Bae,Y.S., Bae,Y.S.,
            Kim,D.K., Kim,Y.S., Yang,S.H. and Kim,H.Y.
  TITLE     Siglec-F-expressing neutrophils are essential for creating a
            profibrotic microenvironment in renal fibrosis
  JOURNAL   J Clin Invest 132 (12) (2022)
   PUBMED   35482420
  REMARK    GeneRIF: Siglec-F-expressing neutrophils are essential for creating
            a profibrotic microenvironment in renal fibrosis.
REFERENCE   5  (bases 1 to 2529)
  AUTHORS   Borkner,L., Curham,L.M., Wilk,M.M., Moran,B. and Mills,K.H.G.
  TITLE     IL-17 mediates protective immunity against nasal infection with
            Bordetella pertussis by mobilizing neutrophils, especially
            Siglec-F+ neutrophils
  JOURNAL   Mucosal Immunol 14 (5), 1183-1202 (2021)
   PUBMED   33976385
  REMARK    GeneRIF: IL-17 mediates protective immunity against nasal infection
            with Bordetella pertussis by mobilizing neutrophils, especially
            Siglec-F(+) neutrophils.
            Erratum:[Mucosal Immunol. 2021 Sep;14(5):1218-1219. doi:
            10.1038/s41385-021-00417-3.. PMID: 34079070]
REFERENCE   6  (bases 1 to 2529)
  AUTHORS   Zhang,M., Angata,T., Cho,J.Y., Miller,M., Broide,D.H. and Varki,A.
  TITLE     Defining the in vivo function of Siglec-F, a CD33-related Siglec
            expressed on mouse eosinophils
  JOURNAL   Blood 109 (10), 4280-4287 (2007)
   PUBMED   17272508
  REMARK    GeneRIF: Negative feedback role for Siglec-F in regulating the
            pathogenesis of human eosinophil-mediated disorders.
REFERENCE   7  (bases 1 to 2529)
  AUTHORS   Angata,T., Margulies,E.H., Green,E.D. and Varki,A.
  TITLE     Large-scale sequencing of the CD33-related Siglec gene cluster in
            five mammalian species reveals rapid evolution by multiple
            mechanisms
  JOURNAL   Proc Natl Acad Sci U S A 101 (36), 13251-13256 (2004)
   PUBMED   15331780
REFERENCE   8  (bases 1 to 2529)
  AUTHORS   Zhang,J.Q., Biedermann,B., Nitschke,L. and Crocker,P.R.
  TITLE     The murine inhibitory receptor mSiglec-E is expressed broadly on
            cells of the innate immune system whereas mSiglec-F is restricted
            to eosinophils
  JOURNAL   Eur J Immunol 34 (4), 1175-1184 (2004)
   PUBMED   15048729
REFERENCE   9  (bases 1 to 2529)
  AUTHORS   Aizawa,H., Zimmermann,N., Carrigan,P.E., Lee,J.J., Rothenberg,M.E.
            and Bochner,B.S.
  TITLE     Molecular analysis of human Siglec-8 orthologs relevant to mouse
            eosinophils: identification of mouse orthologs of Siglec-5
            (mSiglec-F) and Siglec-10 (mSiglec-G)
  JOURNAL   Genomics 82 (5), 521-530 (2003)
   PUBMED   14559209
  REMARK    GeneRIF: We detected expression of mSiglec-5 (or -F), -10, and -E
            mRNA in purified mouse eosinophils
REFERENCE   10 (bases 1 to 2529)
  AUTHORS   Angata,T., Hingorani,R., Varki,N.M. and Varki,A.
  TITLE     Cloning and characterization of a novel mouse Siglec, mSiglec-F:
            differential evolution of the mouse and human (CD33)
            Siglec-3-related gene clusters
  JOURNAL   J Biol Chem 276 (48), 45128-45136 (2001)
   PUBMED   11579105
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB842394.1 and AF293371.1.
            
            On Aug 24, 2012 this sequence version replaced NM_145581.1.
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes a longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF293371.1, AK155469.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                BB842394.1         2-65
            65-2529             AF293371.1         1-2465
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.25 cM"
     gene            1..2529
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /note="sialic acid binding Ig-like lectin F"
                     /db_xref="GeneID:233186"
                     /db_xref="MGI:MGI:2681107"
     exon            1..80
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            81..141
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1814
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sialic acid-binding Ig-like lectin 5; siglec-5;
                     siglec-F; sialic acid-binding lectin Siglec-F"
                     /codon_start=1
                     /product="sialic acid-binding Ig-like lectin 5 isoform 1
                     precursor"
                     /protein_id="NP_663556.1"
                     /db_xref="CCDS:CCDS21167.1"
                     /db_xref="GeneID:233186"
                     /db_xref="MGI:MGI:2681107"
                     /translation="MRWAWLLPLLWAGCLATDGYSLSVTGSVTVQEGLCVFVACQVQY
                     PNSKGPVFGYWFREGANIFSGSPVATNDPQRSVLKEAQGRFYLMGKENSHNCSLDIRD
                     AQKIDTGTYFFRLDGSVKYSFQKSMLSVLVIALTEVPNIQVTSTLVSGNSTKLLCSVP
                     WACEQGTPPIFSWMSSALTSLGHRTTLSSELNLTPRPQDNGTNLTCQVNLPGTGVTVE
                     RTQQLSVIYAPQKMTIRVSWGDDTGTKVLQSGASLQIQEGESLSLVCMADSNPPAVLS
                     WERPTQKPFQLSTPAELQLPRAELEDQGKYICQAQNSQGAQTASVSLSIRSLLQLLGP
                     SCSFEGQGLHCSCSSRAWPAPSLRWRLGEGVLEGNSSNGSFTVKSSSAGQWANSSLIL
                     SMEFSSNHRLSCEAWSDNRVQRATILLVSGPKVSQAGKSETSRGTVLGAIWGAGLMAL
                     LAVCLCLIFFTVKVLRKKSALKVAATKGNHLAKNPASTINSASITSSNIALGYPIQGH
                     LNEPGSQTQKEQPPLATVPDTQKDEPELHYASLSFQGPMPPKPQNTEAMKSVYTEIKI
                     HKC"
     exon            142..504
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            505..783
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            784..831
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            832..1083
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1386
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1486
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1622
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..2510
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2395..2400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
                     /note="hexamer: AATAAA"
     polyA_site      2418
                     /gene="Siglecf"
                     /gene_synonym="mSiglec-F; Siglec5"
ORIGIN      
        1 cttgatagtg ctgataacct gtgggtggaa agttccctct ctgtggtcag tcactctgtc
       61 tctcttcatt cctggactag gaacctagag cctttcactg tgtgatgcgg tgggcatggc
      121 tgctgcccct gctgtgggca gggtgcctgg ctacggacgg ttattcgctc tcagtgactg
      181 gttcagtgac agtgcaggag ggcctgtgcg tctttgtagc ctgccaagtc cagtacccca
      241 attccaaagg tcctgtcttt ggctactggt tccgggaggg ggccaatata ttcagcggct
      301 ctccagtggc tacaaatgac ccacagcgat cagtactgaa ggaagctcaa ggtcgattct
      361 atctcatggg gaaggaaaat agtcataatt gctccctgga catcagagat gcacagaaaa
      421 tcgacacagg gacgtacttc ttcagattgg atggttctgt gaaatacagt tttcagaagt
      481 caatgctctc tgtgcttgtg atagctctga ctgaggtccc taacatccaa gtcacatcta
      541 ccctggtgtc tggcaattct accaaactgc tctgttctgt gccctgggcc tgtgagcagg
      601 ggacaccccc catcttttcc tggatgtcat ctgccctcac ttccctgggc cacaggacca
      661 ccctctcctc agagctgaac ctcacaccca ggcctcagga caatggcacc aacctcacct
      721 gccaggtgaa cttacctggc actggtgtta ctgtggagag gactcaacag ctcagtgtca
      781 tctatgctcc acagaagatg accatcaggg tgtcctgggg agatgacaca ggaactaaag
      841 tcctgcagag tggagcatct ctgcagatcc aagagggtga gtccctgagc ctggtctgta
      901 tggctgacag caatccccct gccgtgctga gttgggagcg cccgacccaa aagcccttcc
      961 agctctctac ccccgcggaa ctgcaactgc cacgggcgga gttggaggac cagggaaaat
     1021 acatctgcca agctcagaac agtcagggtg ctcagacagc ctctgtgagc ctcagcataa
     1081 ggagccttct acagctgctg ggaccctcct gctccttcga gggtcaaggt ctgcactgca
     1141 gctgctcctc cagagcctgg cctgccccct ccctgcgctg gcggctgggg gagggggtgc
     1201 tggaggggaa cagcagcaat ggctccttca cggttaagtc cagctccgca ggacagtggg
     1261 ccaacagctc cctgatcctc agcatggaat tcagctctaa ccacagactc agctgtgaag
     1321 cttggagtga caatagagtt cagagagcca ctatcttgct ggtgtcaggc ccaaaggtct
     1381 cacaggcagg caaatcagaa accagtagag gaacggtcct gggggccatc tggggagctg
     1441 gtcttatggc cttgcttgct gtctgcctct gcctcatctt tttcacagtg aaggtcctca
     1501 ggaagaaatc agccctgaaa gtagcagcta cgaaaggcaa ccatcttgcc aagaaccctg
     1561 cctccaccat aaatagtgca agcataactt catccaacat tgcgttggga tacccaatcc
     1621 agggtcatct gaatgaacca gggtcacaga cccagaaaga acagccaccc cttgccacag
     1681 tcccagacac ccaaaaggat gagcctgaac tccactatgc ctccctctcc ttccaaggcc
     1741 cgatgccccc gaagcctcag aacacggagg ctatgaaatc tgtatacaca gagatcaaga
     1801 tccacaagtg ctgaagttct ttgctccatg tgtcccagtg ctggctggag tcccagaaag
     1861 gcatggccaa ggacagcaag tgttagaagc atattgtgat atcagtggcc ctctaaacca
     1921 aaggctatgt agtggaagga gggagttttg gaaggaatga aggtctcaga gatttctagg
     1981 ttcaattcca gattcagtgt tatccccaga gaagccacct tttctctttg tgccctctct
     2041 tcctcttctg taataataat cacaagggtc ttaatcatga ggagtgtcaa ggagaaagtg
     2101 tagtgtttat tgttgattat ttggctttat attgcctgag cagtgtggaa gaacattttg
     2161 actttaatgc atacttgaat tttacttgta tatatttaag aacatatgtt agttcctaaa
     2221 gttattctgt tttgaagtgt gactgtgttg tagctacata tatctcaaat tgaactcata
     2281 ttggatgttc tgcaaacttt taaagatcac acagatgcca gcccagacta ctatacccag
     2341 caaaattatc aatcacaggg cataaaatta atatataaag atttatcaca aatcaataaa
     2401 agtgacagat aatcaataca aaatggtata tgtgagtggc cagcaaacat acaagaaggt
     2461 aatctcagtc agtgcttgtc aatgaaatat aaataaatac aatacaacta aaaaaaaaaa
     2521 aaaaaaaaa
//
