LOCUS       NM_001040459            8278 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus shroom family member 4 (Shroom4), transcript variant
            1, mRNA.
ACCESSION   NM_001040459 XM_205522 XM_906634 XM_975890 XM_975929 XM_976035
VERSION     NM_001040459.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Kolvenbach,C.M., Felger,T., Schierbaum,L., Thiffault,I.,
            Pastinen,T., Szczepanska,M., Zaniew,M., Adamczyk,P., Bayat,A.,
            Yilmaz,O., Lindenberg,T.T., Thiele,H., Hildebrandt,F.,
            Hinderhofer,K., Moog,U., Hilger,A.C., Sullivan,B., Bartik,L.,
            Gnys,P., Grote,P., Odermatt,B., Reutter,H.M. and Dworschak,G.C.
  TITLE     X-linked variations in SHROOM4 are implicated in congenital
            anomalies of the urinary tract and the anorectal, cardiovascular
            and central nervous systems
  JOURNAL   J Med Genet 60 (6), 587-596 (2023)
   PUBMED   36379543
  REMARK    GeneRIF: X-linked variations in SHROOM4 are implicated in
            congenital anomalies of the urinary tract and the anorectal,
            cardiovascular and central nervous systems.
REFERENCE   3  (bases 1 to 8278)
  AUTHORS   Tian,R., Guo,K., Wu,B. and Wang,H.
  TITLE     Overexpression of Shrm4 promotes proliferation and differentiation
            of neural stem cells through activation of GABA signaling pathway
  JOURNAL   Mol Cell Biochem 463 (1-2), 115-126 (2020)
   PUBMED   31654185
  REMARK    GeneRIF: the overexpression of Shrm4 or activation of GABAB1
            upregulated the levels of Shrm4, GABAB1, Bcl-2, MAP-2 and SOCS2,
            while downregulated Bax and cleaved Caspase-3 in NSCs. Overall,
            overexpression of Shrm4 activated GABAB1 to stimulate the
            proliferation and differentiation of NSCs.
            Erratum:[Mol Cell Biochem. 2020 Sep;472(1-2):255-257. doi:
            10.1007/s11010-020-03786-8.. PMID: 32583147]
REFERENCE   4  (bases 1 to 8278)
  AUTHORS   Etournay,R., Zwaenepoel,I., Perfettini,I., Legrain,P., Petit,C. and
            El-Amraoui,A.
  TITLE     Shroom2, a myosin-VIIa- and actin-binding protein, directly
            interacts with ZO-1 at tight junctions
  JOURNAL   J Cell Sci 120 (Pt 16), 2838-2850 (2007)
   PUBMED   17666436
REFERENCE   5  (bases 1 to 8278)
  AUTHORS   Yoder,M. and Hildebrand,J.D.
  TITLE     Shroom4 (Kiaa1202) is an actin-associated protein implicated in
            cytoskeletal organization
  JOURNAL   Cell Motil Cytoskeleton 64 (1), 49-63 (2007)
   PUBMED   17009331
REFERENCE   6  (bases 1 to 8278)
  AUTHORS   Dietz,M.L., Bernaciak,T.M., Vendetti,F., Kielec,J.M. and
            Hildebrand,J.D.
  TITLE     Differential actin-dependent localization modulates the
            evolutionarily conserved activity of Shroom family proteins
  JOURNAL   J Biol Chem 281 (29), 20542-20554 (2006)
   PUBMED   16684770
REFERENCE   7  (bases 1 to 8278)
  AUTHORS   Hagens,O., Ballabio,A., Kalscheuer,V., Kraehenbuhl,J.P.,
            Schiaffino,M.V., Smith,P., Staub,O., Hildebrand,J. and
            Wallingford,J.B.
  TITLE     A new standard nomenclature for proteins related to Apx and Shroom
  JOURNAL   BMC Cell Biol 7, 18 (2006)
   PUBMED   16615870
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 8278)
  AUTHORS   Hagens,O., Dubos,A., Abidi,F., Barbi,G., Van Zutven,L.,
            Hoeltzenbein,M., Tommerup,N., Moraine,C., Fryns,J.P., Chelly,J.,
            van Bokhoven,H., Gecz,J., Dollfus,H., Ropers,H.H., Schwartz,C.E.,
            de Cassia Stocco Dos Santos,R., Kalscheuer,V. and Hanauer,A.
  TITLE     Disruptions of the novel KIAA1202 gene are associated with X-linked
            mental retardation
  JOURNAL   Hum Genet 118 (5), 578-590 (2006)
   PUBMED   16249884
REFERENCE   9  (bases 1 to 8278)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC151022.1 and AL671501.8.
            
            On Sep 1, 2015 this sequence version replaced NM_001040459.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC151022.1, DQ435687.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4661              BC151022.1         179-4839
            4662-8278           AL671501.8         76621-80237
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 2.85 cM"
     gene            1..8278
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /note="shroom family member 4"
                     /db_xref="GeneID:208431"
                     /db_xref="MGI:MGI:2685570"
     exon            1..230
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..4541
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein Shroom4"
                     /codon_start=1
                     /product="protein Shroom4 isoform 1"
                     /protein_id="NP_001035549.1"
                     /db_xref="CCDS:CCDS29959.1"
                     /db_xref="GeneID:208431"
                     /db_xref="MGI:MGI:2685570"
                     /translation="MESRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGG
                     KAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNTPVSRPHSWH
                     VAKLLEGCPDVATTMHFPSEAFSLSWHSGCNTSDVSVQWCPLSRHCSTEKSSSIGSME
                     SLEQPGQPTYEGHLLPIDQNMYPSQRDSAYSSFSASSNASDCALSLKPEEPPSTDCVM
                     PGPGPIKVTDDQANVSENSGSSHSTSEDHVTSTSHASSYSDEGHHSGPAKMARGPPEP
                     PVRSDSLPASRAQLLNGEQHRASEPVDSLPQKEKPGLETVLPPRSSNQFCCLSGQDQV
                     TDEDHQNCELSKPSESSQDDCEHLLIEDSSKALDSPKAHDKGSNKEFGLLKEASADLA
                     NTLNFGAIPHLRGTMEHRHSAPEQLLASHLQQVHLDSRGSKGMELPIGQDGHQWTVSP
                     LHNNPKGKKSPSLPTGGTQDQTRKERKTTPLDDKLMASVHQSQSDVLLGEVDGHPNRA
                     GRASSDLTSQQPSATCSSVQQTRDFLSAHKIVDHTEASEEGDNEPKECGRLGGRRSGG
                     PRGRSIQNRRRSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTNEAVEETQEPPESPP
                     LSASNASLLPSYKNVPSPGDKVFNKSMILRARSSECLSQASESSKARGGVEGRMSPGQ
                     RSGQSSLALNTWWKASDSSTLDTEKANAHHGVCRGHWRWSPEHNAQPQVALSTEAPSN
                     PDDSKELKTSTPQAGEEAVLMPFADRRKFFEESSKSLSTSHLPGLTTHNNKPFIQRQK
                     PIDQNFQSVSYRDLRCHPLDQSYHSADQSYHAADQSYHSLSPLQSETPTYPECFATKG
                     RDNSLCCKPVHHGDCDYHRTCSHPCSAQGTVRHDPCICCSGEICPALLKRNLLPKCHN
                     CRCHHHQCIRCTGCCHGPQHSAHEDSSMAPGNAWKSRKAAIQEFPVDKWKPITGNRKT
                     SHSGREMAHSKAGFSLSTPFRPCIENPALDLSNYRAVSSLDILGDFKRASNKPEESSV
                     YEDENSVASMPRPLRSRAFSESHISLEPQNTQAWGKHQRESFSKGSETQPDTLGARKK
                     VFPPPRPPPPNWEKYRLFRAAQLQQQQQQQQQQQQQQRCEEEEEKEQEEEGEKEEDLP
                     PQYFSSELTGSCAPNTEEQPQSLKMGHQEASRQGSQSLQEQEAFALHPSNFVPPVRGC
                     TVPQPEKAQHPCYYGTHGLWRTTEQEATVTPKQEFQHFSPPKGASGIPTSYSAYYNIS
                     VAKAELLNKLKQQPEMAEAGLGEEGVDYELAQKKIQLIESISRKLSVLREAQRGLLDD
                     INANAALGEEVEANLKAVCKSNEFEKYHLFIGDLDKVVNLLLSLSGRLARVENALNSI
                     DSESNQEKLVLIEKKQQLTNQLADAKELKEHVDGREKLVFGMVSRYLPQDQLQDYQHF
                     VKMKSALIIEQRELEEKIKLGEEQLKCLKESLHLGPSNF"
     exon            231..382
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            383..517
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            518..2981
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..3043
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..3820
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            3821..4001
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            4002..4271
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            4272..8278
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8254..8259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /note="hexamer: AATAAA"
     polyA_site      8276
                     /gene="Shroom4"
                     /gene_synonym="D430043L16Rik; Gm724; Shrm4"
                     /note="major polyA site"
ORIGIN      
        1 ggggcggcgc ggcgcggcgc aggaggacga tggtggctga gctgctgggg gcggaggctc
       61 cgtagccccc gcggaaaagg agcccggcgg cgcctgccca gcccgagccg aggatggaga
      121 gccggccggg gtccttccag tacgtcccgg tgcagctgca agggggggcg ccctggggct
      181 tcaccctcaa ggggggcttg gagcactgcg agccgctcac ggtgtcgaag attgaagatg
      241 gaggcaaggc agccttgtct cagaagatgc ggactggtga tgagctggtg aatatcaatg
      301 gcactccatt gtatggctcc cgccaagagg ccctcatcct tatcaaaggc tccttcagga
      361 tcctcaagct gattgtcagg aggagaaaca cccctgtgag taggccgcac tcatggcatg
      421 tagccaagct gttggagggg tgccctgacg tggccaccac catgcatttc ccttctgaag
      481 ccttcagctt gtcctggcat tccggttgca acactagtga cgtgagtgta cagtggtgcc
      541 ccctctcccg gcattgcagc actgagaaga gcagctctat tggcagcatg gaaagcctgg
      601 aacagccagg ccaacctacc tacgaaggcc acctcttacc cattgaccag aacatgtacc
      661 ccagccagcg tgactcagcc tatagctcct tctcagccag ctcaaatgcc tctgactgtg
      721 ccctttccct taagccagag gaaccaccct ctacagactg tgtcatgcca ggcccagggc
      781 cgattaaagt cactgatgac caagctaatg tatctgagaa ctcaggaagt agccacagta
      841 ccagtgagga ccatgtgacc tccacttccc atgcatcatc ctactcagac gagggccacc
      901 actcaggacc tgccaagatg gcacggggtc caccagagcc tccagtgagg agcgacagcc
      961 ttccggcctc tagagcccaa cttctcaatg gagagcagca tcgtgcatct gaaccggtgg
     1021 actccttacc acaaaaggaa aaaccaggct tagagacagt gctgccccca aggagctcta
     1081 atcagttctg ctgtcttagt gggcaagacc aagtgacaga tgaggaccac cagaactgtg
     1141 agctcagcaa gccttctgaa tccagccagg atgactgtga gcatctactg atagaggact
     1201 cctccaaagc tcttgattcc cccaaagccc atgacaaagg ttccaacaaa gagtttggcc
     1261 tgcttaagga ggcttcagca gatttggcta acactttgaa ctttggtgcc attccgcacc
     1321 tccgaggaac catggaacat cgccatagtg ctcctgaaca gctgttggca tcccacttgc
     1381 agcaggtgca ccttgattcc agaggcagca aggggatgga gttgccaatt gggcaggatg
     1441 gacaccagtg gactgtgtcc cctttgcaca acaaccctaa agggaagaaa agtccaagtc
     1501 ttcccacggg aggcacccag gaccagaccc gtaaagaaag gaagaccacg cccctggatg
     1561 ataaactgat ggcttctgta caccagagcc aaagtgatgt cctacttggg gaggttgatg
     1621 gacacccaaa ccgagcaggc agagcaagca gtgacttgac tagccagcag ccctctgcca
     1681 cttgttcctc tgttcaacaa accagagact tcctttcagc ccataaaata gttgatcaca
     1741 cagaagcaag tgaagaaggt gacaatgagc ctaaggagtg tggccgattg ggtggcagaa
     1801 gaagtggagg cccccggggc cgctccatcc aaaaccgacg gaggagtgag aggtttgcta
     1861 ccaatctacg gaatgaaatt cagaggagga aagcccagct ccagaaaagt aagggcccgt
     1921 tgtcacaatt gtgtgacacg aacgaagccg tggaagagac ccaagagcct ccagaaagtc
     1981 ctccgctctc tgcttctaac gcatctcttc tgccttcata taaaaacgtc cctagtcctg
     2041 gagacaaggt ctttaacaaa agcatgattc tcagggctag atcttcagaa tgcctcagcc
     2101 aagcctcaga gagttccaaa gctaggggag gcgtagaggg aagaatgagc cctggccaga
     2161 ggtctggcca gtcttctttg gccctgaaca catggtggaa agcatctgac tcatccacct
     2221 tagacactga gaaagcaaat gctcaccatg gggtttgtag aggccattgg agatggtctc
     2281 cagaacataa cgcacagcca caggtggcac tatccacaga agcgccttcc aacccagatg
     2341 acagcaaaga gttgaagaca tctactcctc aagctgggga agaagctgtc ctcatgccgt
     2401 ttgcagacag aagaaagttc tttgaagaga gcagcaaatc tttatctaca tcacatttgc
     2461 caggcttaac cactcataac aacaagccat ttatccagag acaaaaacct atagatcaaa
     2521 acttccagtc agtgagctat agggatttaa gatgccatcc tctggaccaa tcatatcatt
     2581 ctgcagacca atcataccat gctgcagacc aatcctatca ttccttgtca ccacttcagt
     2641 cagaaactcc tacttaccca gaatgctttg ctactaaagg ccgagacaat tccctgtgct
     2701 gtaagccagt gcatcacggg gactgtgatt accacaggac ctgttctcat ccctgcagtg
     2761 ctcagggaac tgtaaggcat gatccttgca tctgttgttc tggagaaatc tgccctgcct
     2821 tgctaaagag aaacttgttg ccaaaatgcc acaactgtag gtgtcaccac caccaatgca
     2881 tccggtgtac aggttgctgt cacggtcctc agcatagtgc ccatgaggac agcagcatgg
     2941 cacctggcaa tgcttggaaa tccaggaagg cagccataca ggaatttcct gtggacaagt
     3001 ggaagccaat aacaggaaac aggaaaacta gtcattctgg gagggaaatg gctcattcta
     3061 aggccggctt ttcattgtct actcccttcc gaccttgcat tgagaatcca gcactggact
     3121 tgtcaaacta ccgagcagtc tcttctcttg acatccttgg agacttcaaa cgtgcctcaa
     3181 acaagccaga ggaatcttcg gtttatgagg atgagaactc tgttgcctcc atgccccgcc
     3241 cactgcggag ccgagccttc tctgagagtc acatcagttt agagcctcaa aacacccagg
     3301 cctggggaaa acatcagaga gaatcattta gtaaaggcag tgagactcag ccagatacac
     3361 ttggggcccg aaagaaggtc tttcctcctc ctcgccctcc tcctcccaac tgggaaaaat
     3421 ataggctctt tcgtgcagca cagctgcagc agcagcagca gcaacaacaa caacaacaac
     3481 aacaacagcg atgtgaagag gaagaagaga aggagcaaga ggaggaagga gagaaagagg
     3541 aggacctacc accccagtat tttagttcag aactcactgg ctcatgtgcc cctaatacag
     3601 aggaacagcc acagtccctg aagatgggcc accaggaagc ctcaaggcag ggttcacaga
     3661 gcctccaaga gcaagaggcc tttgctctgc atcctagcaa ttttgtgcct ccagtaaggg
     3721 gctgcactgt accccagcct gagaaagctc aacatccttg ctattatggc actcatggac
     3781 tatggaggac aactgaacaa gaagctactg tgaccccaaa acaagagttt cagcacttct
     3841 cacctcctaa gggggcctca ggaatcccta cctcttactc agcttattat aatatctctg
     3901 tggccaaggc agaactgctg aacaagttaa aacagcagcc tgagatggca gaggcaggcc
     3961 tgggagagga gggagttgac tatgaactgg ctcaaaaaaa gattcagctc attgaaagca
     4021 ttagtcgaaa actttctgtc ctgcgggagg cccagcgcgg gctactggat gacatcaatg
     4081 ccaatgctgc ccttggagag gaggtagagg ccaacttaaa agctgtctgc aaatccaatg
     4141 agtttgaaaa gtaccatttg tttattggag acctggacaa agtggtcaac ctgttgttgt
     4201 cactctctgg acgactagcc cgggtggaga atgctctcaa cagcattgat tcagagtcca
     4261 accaggagaa gttggtgctg atagagaaga agcagcagct gacgaaccag ttggcagatg
     4321 ccaaggagtt gaaggagcat gtggatggca gggaaaagct ggtgttcggc atggtctccc
     4381 gctacctgcc tcaggaccag ctccaagatt accagcactt tgtgaagatg aaatctgctc
     4441 tcatcattga acagagagag ctggaggaga agatcaaact tggagaagag cagcttaaat
     4501 gcctcaaaga gagcctgcac ttggggccca gcaatttcta attgcaccag gagtatgcca
     4561 ctgcacctca gtctattccc ggtggaaaag ggctttcaat cttcagaagc agtttgctag
     4621 ccaagtatat agacattact tagcagtgag tccagccatc taccttggcc agccatcaag
     4681 gagaggctgg gaaggcccca agcttctctt acagagctgc agcaaggtgt ggccacacta
     4741 ctatgctccc agttagcaag gattaaaaag tgccttcatc tctgtactaa aacaccaaag
     4801 acttcacaaa ctcaagactc tcccttacca cactaccctc ttgaggttca aagctaaact
     4861 gcctgtgcaa tttcccctct tccctaggga tagtgaccta gctagccatt gtccacacag
     4921 atgtgcttga cttgaataca ttcacctcac accagttcca gggcctctac caaatgtggc
     4981 agcaggaggc acagaagggg agacttcaca taaggagagc aagcatggag tccacccgag
     5041 tattcttccc tctggtaata gccaggctta aacaaattgg ttccatgtgt accctcccca
     5101 acacctagtt gatagctttg ttaccgcggc tttcgccctg ttttcacatg atgatagacc
     5161 tgcctctttg tattgtaacc tgtcctgata tcactaggag tctcgtgcca gggtttcact
     5221 ctctgattct ctaacgtatc tctattatag ttgactttgc aatacagctt cgttactggg
     5281 gaagagggtt cttatcatat ttcaaatggt ctttaatggt attgattttt atgttttctt
     5341 tttctttctt aactaatgag gtgagaaaaa acaagttagg cagaaggaaa atataagtct
     5401 gagcccaccg tggctaagga agcaagcgcc aaagccttga attcttccta ccatagttcc
     5461 gagtagctat taagtttctc tccttggact tagagcctcc taaccatagc tcagctatgt
     5521 gcttccttga gggctggatg actataacct taacattcct gagtagggtt cagtgttttt
     5581 taactgcctt ttgaaaccag agtctcctat aactgaaaga tacaggatac atgaaggaag
     5641 gaaggtgggt gggtgtggaa ggcgctcttc taacaactgc tttcccagcc agtggctagc
     5701 tcaaggaacc aaaaccatga gagtattttc agccagggcc aggcctcgga gcaaattatg
     5761 attcacccag gatctagaca tacactatcc ttccttgtct tcggggccta tgtactaaag
     5821 catcccttcg ggtggatgcc tgaaatcctc aatagtacca atgcttgtaa atagcacaat
     5881 tgttaatctc atcactgagg ggactaataa gtgactaaag aaaggctgcc tgtatagcat
     5941 ggatacactg gataaatata tgattgatgg tccatgtggg atggagcaag ctttcatcac
     6001 actgcacaga atggcacata atttaaaact gatcagccat ttgactctac gatgttccat
     6061 ttaatattgt caaccatggt tgagcatgag aacggaaact ctggaaagtc aaactgcagg
     6121 taagagggat tgatgcgcaa agcactaaag ctgttgctct cgccaagacc tgacgttggt
     6181 agccagcttc tatcctggaa aagcatccaa gtgagcaata gcaagagggg actcatcacc
     6241 aaaccgccct tctatcttgc tcagtcttct gttccctact aactgggtac attccttgat
     6301 gatgtcgcct aacgatctct caaaagccca aaacaaccaa gccctggcat caccacctgt
     6361 cacagctgag ttcctcaggg tccttggaca ttccctgtag cttcagtgtt actttattca
     6421 accaaagaat tggccaagga acttcacagc tgctacaaat accggccact ctcttccatt
     6481 ctctgcacac tgtgtctgac agctatggga gaatcttggc tggcgaggta ttctaaaggg
     6541 atgggagcct tttctatgag ccattgggta tgctttttag tactgctgcc tctgcaataa
     6601 gcactgttta tctctcagta ctgccattct cccctcaagt gggagatcac aaattatctt
     6661 caacatgact ctaaaagtat actgagcgct gaagctcagt tgctgctatg ctatgctatg
     6721 gtgtaccaca aagtactgtg cagcgctatg ctgcgttatg ctgttctgtg cttttctgtt
     6781 cagaatggct gagagtttac ccaaagtaaa attgagaggg gtgaggaact gtttgaataa
     6841 aagccacact tgccttacct tttttaaata taatgggccc taggacaatc aataaggaag
     6901 ttgaaatgtc ttatactaga atagtgagag ttgtgtgttc tgtcattcca agatgtgttt
     6961 ggctacagta gaacaaggaa cattcacttt actggatatc atggccatag gctgggtatt
     7021 tgtagccttc gttaggttta ttatacctta agtatatgat accaaaaata ctgttaactg
     7081 tggtttgtgt gtgtttgttt gtttgttgtg ttggcattgt tagagatgga gcccaacacc
     7141 ttatatatac tgggcaagtg tatcactgag ctatagcccc agccttaatt ctcagttttt
     7201 atcacttgcc ccacctcaaa atggtttata catttggaat cagaatacac ggctgagttt
     7261 ccaaagtatt tgaaagataa cttcacaact atacagagct caatagactg aactaacact
     7321 ggtatgccct actcctgcta tggccttccc aaagtgagaa tacaaaatgc cagtgctatt
     7381 ggcatgggtt cattctcttt ctctctctct ctctctctct ctctctctct ctctctctct
     7441 ctctctcgtc ttgccttctg ggatcagcca tctttcaagg cctctgcgaa gatagcagag
     7501 cggcatggaa gtacgttcag aggttgaccc accccctcaa aggttgctga tcatctactc
     7561 tttcccttca aataaatctc attggataat acaaaagcag ggtggtggat acaagggaat
     7621 caatgcaaga ttgtgatcat atgcctgttc ttctcccatc cccagggctg taggtaattg
     7681 actcccctca agtactacta ttgaaggctc agctaagaca taaaagtaaa tacccaagtg
     7741 gagtctaaag gaatgggata gctttactga aattaaagtc ctaaaaaggg agaaattgga
     7801 tgcacaggaa aggaaagaga gtaaaaatat gaaaagcctc tttgaagatt ggcttcatac
     7861 ttctgtttgc catttaacct catatgccaa gaaaggtgcc taaggggtac cagacttttc
     7921 taataccaag cctccactag aggaagttgg gtgtacttct ggctatgtct acccatgtgg
     7981 aatttggtag ctctttcttt gccagaggag ctatgtatgg cagtaataat tatgtggcag
     8041 aatattgtat tatttgaaga ttaaatcctt agaagggggc agggtgtggg gcagtgtata
     8101 taaagagtca gtgtttgctg gacaatttcg gggaattgtc tatcccattt aatgctatta
     8161 tttttctggg tcttactaca tttgtgtcct ccaccacttc cttcttggct gacgttgata
     8221 tgcctgccat attgtcatca aggaccatac ttcaataaaa ggtgctaagg acaaaagg
//
