LOCUS       NM_027917               3312 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus shroom family member 1 (Shroom1), transcript variant
            1, mRNA.
ACCESSION   NM_027917
VERSION     NM_027917.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ahuja,N., Hiltabidle,M.S., Rajasekhar,H., Voss,S., Lu,S.Z.,
            Barlow,H.R., Cowdin,M.A., Daniel,E., Vaddaraju,V., Anandakumar,T.,
            Black,E., Cleaver,O. and Maynard,C.
  TITLE     Endothelial Cyp26b1 restrains murine heart valve growth during
            development
  JOURNAL   Dev Biol 486, 81-95 (2022)
   PUBMED   35364055
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Zhao,Z., Zhang,H., Xiong,T., Wang,J., Yang,D., Zhu,D., Li,J.,
            Yang,Y., Sun,C., Zhao,Y. and Xi,J.J.
  TITLE     Suppression of SHROOM1 Improves In Vitro and In Vivo Gene
            Integration by Promoting Homology-Directed Repair
  JOURNAL   Int J Mol Sci 21 (16), 5821 (2020)
   PUBMED   32823670
  REMARK    GeneRIF: Suppression of SHROOM1 Improves In Vitro and In Vivo Gene
            Integration by Promoting Homology-Directed Repair.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3312)
  AUTHORS   Mohan,S., Rizaldy,R., Das,D., Bauer,R.J., Heroux,A.,
            Trakselis,M.A., Hildebrand,J.D. and VanDemark,A.P.
  TITLE     Structure of Shroom domain 2 reveals a three-segmented coiled-coil
            required for dimerization, Rock binding, and apical constriction
  JOURNAL   Mol Biol Cell 23 (11), 2131-2142 (2012)
   PUBMED   22493320
  REMARK    GeneRIF: The structure of Shrm domain 2 (SD2), which mediates the
            interaction with Rock and is required for Shrm function, is
            reported.
REFERENCE   4  (bases 1 to 3312)
  AUTHORS   Lee,C., Le,M.P. and Wallingford,J.B.
  TITLE     The shroom family proteins play broad roles in the morphogenesis of
            thickened epithelial sheets
  JOURNAL   Dev Dyn 238 (6), 1480-1491 (2009)
   PUBMED   19384856
REFERENCE   5  (bases 1 to 3312)
  AUTHORS   Sevilla-Perez,J., Konigshoff,M., Kwapiszewska,G., Amarie,O.V.,
            Seeger,W., Weissmann,N., Schermuly,R.T., Morty,R.E. and
            Eickelberg,O.
  TITLE     Shroom expression is attenuated in pulmonary arterial hypertension
  JOURNAL   Eur Respir J 32 (4), 871-880 (2008)
   PUBMED   18550613
  REMARK    GeneRIF: Shroom localises exclusively to pulmonary smooth muscle
            cells and is attenuated in pulmonary arterial hypertension
REFERENCE   6  (bases 1 to 3312)
  AUTHORS   Etournay,R., Zwaenepoel,I., Perfettini,I., Legrain,P., Petit,C. and
            El-Amraoui,A.
  TITLE     Shroom2, a myosin-VIIa- and actin-binding protein, directly
            interacts with ZO-1 at tight junctions
  JOURNAL   J Cell Sci 120 (Pt 16), 2838-2850 (2007)
   PUBMED   17666436
REFERENCE   7  (bases 1 to 3312)
  AUTHORS   Dietz,M.L., Bernaciak,T.M., Vendetti,F., Kielec,J.M. and
            Hildebrand,J.D.
  TITLE     Differential actin-dependent localization modulates the
            evolutionarily conserved activity of Shroom family proteins
  JOURNAL   J Biol Chem 281 (29), 20542-20554 (2006)
   PUBMED   16684770
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596095.18, AK134332.1 and AK035462.1.
            
            On Mar 20, 2014 this sequence version replaced NM_027917.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            CCDS Note: The coding region has been updated to include an
            additional internal exon that is better supported by transcripts
            from the reference genome strain, and is also supported by homology
            data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134332.1, SRR7345562.3041298.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                AL596095.18        13197-13269
            74-2350             AK134332.1         2-2278
            2351-3113           AK035462.1         3601-4363
            3114-3312           AK134332.1         3044-3242
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 31.95 cM"
     gene            1..3312
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /note="shroom family member 1"
                     /db_xref="GeneID:71774"
                     /db_xref="MGI:MGI:1919024"
     exon            1..137
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            138..1108
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..2641
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein Shroom1"
                     /codon_start=1
                     /product="protein Shroom1 isoform 1"
                     /protein_id="NP_082193.2"
                     /db_xref="CCDS:CCDS24680.2"
                     /db_xref="GeneID:71774"
                     /db_xref="MGI:MGI:1919024"
                     /translation="MEALGTGRDRTSQASATESLDLRRLSTRADSAYSSFSTASGDPE
                     TRTPSPGTERLPYLDWDYVRVVWGSQSPTSKDAVLSTTQRPVQAVAGHSDPRSPEVQG
                     SPGPLNRQDTPLLYALAAEAEATAHTAEPPSPPASRDAYRQRLQGAQRRVLRETSFQR
                     KEFRMSLPGRLRPAVPTRLPTAHVRSASSSQELGEEEPARTAVPALAAAGRGRLSSQQ
                     RQCCFSEPGKLHRVGWSGGPTGEDLRKDYSTQELQRGMHAKSKGLLETQSLSSTELNS
                     GPADLGNAHRPAGRSQSVSGEVMGPCKGSERTVATVQAVPQRADIRRPLLHTKLSRSL
                     TQKEVTGVCPGEALQTKPAGCGRRISETSVSTPGPSLPEDDVFLREAKTPSPQDSQGL
                     PTSTSYRQYENDLSKKAGQIAVSAERPLHETPGITGTEDCGQAVNGSVDLSRPTSIPE
                     TTNDDIPTFDTNGTTDPSAATEKKPLKPPPVDVLRPSDSETPGSPHHTSLTWGQFDSK
                     TTWPSRHFEALVQELARLDPSLSRTLAAQPGPEPPQGLLDGLFPVEEIRSAMRPALEE
                     MGEKAAGASEEGSCGHHLTQDLQTSQEASRSENSTPDPDQSSGQEFPEGNSTQAKKVE
                     LARLLQKMLQDLHAEQERLRGTAADWTQRNGALEAAVSQACTPRELERFRRFMTDLER
                     VLGLLLLLGSRLVRVNLALARAGSNSDPDERASLLQRLQLLQRQQEEAKELKEHVARR
                     EQTLRQVLERELPAEHLRSYCVLLASKARILSQQRSLDDRIRFLKDQLDTIWSDLSHH
                     PLSPRLTWAPAIRPLNKQPFLATLI"
     exon            1109..1164
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1353
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1902
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2035
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2308
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..3312
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2844
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /note="major polyA site"
     regulatory      3295..3300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
                     /note="hexamer: AATAAA"
     polyA_site      3311
                     /gene="Shroom1"
                     /gene_synonym="Apx; Shrm1"
ORIGIN      
        1 gcccctcctc ggccggaggt ggcgcctccc ctgctgcggc cggagactgg aggtggcgtc
       61 gggagctgga gcccagggct cggatgtcac tgagggatcg ccggcgccct gaagtgcgtc
      121 tcgggctgga gccacaggtc tgccaactcc aatttccaac tgtataagca tggaggccct
      181 gggtactggg agagaccgca cctcccaggc ctcagccact gaaagcctgg acttgcgacg
      241 gttgtccacg cgcgccgact ctgcctacag ctctttctcc acggcgtctg gtgatcctga
      301 gacgcgcact ccgtcccctg gcaccgagcg cctcccttac ctagactggg actacgtgcg
      361 agtggtttgg ggcagccaat ctcctacctc gaaggacgct gtcctttcaa cgactcagcg
      421 acctgtgcag gcagtcgctg ggcacagtga cccacggtcc ccagaggtcc agggaagccc
      481 gggaccacta aacagacaag acaccccact gctgtacgcg ctggccgcgg aggctgaagc
      541 tacagctcac accgcagagc cgcccagccc gccagcctcg cgggacgcct accgccagcg
      601 gttgcagggt gcacagcgac gagtgctgcg ggagacgtcc ttccagcgaa aggagtttcg
      661 catgagcctg ccgggccgcc tgcgtcccgc agtccccacg cgacttccca cggcgcacgt
      721 gcgctccgcc tccagcagcc aggagctggg agaagaggag ccggcgcgca ccgctgtccc
      781 agcgctagct gctgctggcc gggggcgtct ctccagccag cagcggcagt gctgcttctc
      841 tgagccaggc aaattacatc gcgtaggttg gagcggtggg cccactggtg aggacttgag
      901 aaaagattat tccacgcagg agttgcagcg tgggatgcac gcaaagtcca aagggctgct
      961 ggagactcag tccctaagct caacggagct gaattccggg ccagcggatc ttggcaatgc
     1021 ccatagacct gcgggtcgga gtcagagtgt ttcaggcgag gtcatgggtc cctgcaaggg
     1081 ttcagaaagg actgtggcca ctgtccaggc tgttcctcaa agagcagaca tccgcagacc
     1141 actgcttcac accaagcttt ccaggtcttt gactcagaag gaggtcacag gagtgtgtcc
     1201 tggagaggcc ctccagacca aaccggctgg ctgtggtcgg agaatctctg agacctcggt
     1261 gtctactccg ggcccctccc tccctgaaga tgacgtgttc ctgagagaag ccaaaacacc
     1321 gtcacctcaa gattcccaag ggctccccac cagcacctcc taccggcagt atgaaaacga
     1381 cttaagcaaa aaagctggtc agattgcagt ttcagcagag agacccctcc atgagactcc
     1441 agggatcacg ggtacagagg actgtgggca agcggtgaac ggctctgtgg atctctccag
     1501 acccacaagc attccagaga ctacaaatga cgacatccca acctttgaca ctaatgggac
     1561 cactgacccc tctgcagcta cagagaaaaa acccctcaag cctcccccag tcgatgtctt
     1621 gagaccttca gactctgaga ctccagggtc ccctcaccac acttccctga cttggggcca
     1681 gtttgattcc aagactactt ggcctagtcg gcattttgag gcgctggttc aagagctggc
     1741 cagactggat ccctctttga gccgtactct tgccgcccag cccggtccag agccaccgca
     1801 gggcctgctg gatgggcttt ttcctgtcga agagatccga agtgcgatga ggccagccct
     1861 tgaggagatg ggagagaagg ctgctggcgc atcggaggaa gggtcttgtg gacaccacct
     1921 cacgcaggac ctgcagactt cccaagaggc atcaaggtct gaaaactcta cccccgaccc
     1981 tgaccaatca agtggccagg aattcccaga aggaaacagc acccaggcca agaaagtgga
     2041 actagcccgc ctcctccaaa agatgctgca ggatcttcac gctgagcagg agcgactgcg
     2101 ggggaccgcc gcagactgga cccaacgcaa cggagctcta gaggctgctg tgagccaggc
     2161 ctgtacaccc cgggagctgg aacggttccg ccggttcatg actgatctag agcgtgtgct
     2221 tggtctgctg ctgctgctag gcagtcgcct ggtccgtgta aacctcgcct tggctagggc
     2281 gggctcaaac agcgaccctg atgagagggc ctctctgctg cagcgactcc aacttctgca
     2341 gcgacagcaa gaagaagcta aggagctgaa ggagcacgtg gctcggcggg agcaaaccct
     2401 gcgtcaggtg ttggagcggg agctgcccgc agagcatctg cgctcctatt gtgtgctgct
     2461 ggcctccaag gccaggatcc tgtcacagca gcgcagcctg gacgatcgaa tccggttcct
     2521 taaggaccaa ctggacacta tctggagcga cctcagccat catccccttt ctcccagact
     2581 gacctgggcc ccagcaatcc gtcctctaaa caaacaacct tttcttgcta cccttatcta
     2641 gttacatgta gcggaggtgg gaacattgtc ccaggctcac ccacaactgg ggcgatgctt
     2701 tatagagagc tttcccttga gggagtaggg aggagtcaga ccaggccttc ctaggacaca
     2761 tcttcccacg gtgttctcct gtgaccttat tcaagtttgt aattctttgg gaatttgtgg
     2821 gtcgtaaaaa ctgcagtatt caaagcctgg cctggtggtg tagctgtact ccccgctata
     2881 tgggaagctg aagatttcaa gtgtaaggtc tatccgggct acaaagggaa ctcaaagcca
     2941 gcctggacaa cttaggagac tctccctcaa aataagacgt aaaaagaggg ctgcagggtt
     3001 tcgggttagg tatacagggt tgggggaggt ctgcctccac aaaagacaga aaaaagaagt
     3061 ctgcaggggt gggagttggg gatacagggt tgggggtgtg tgtgtgtgtg aagagatgga
     3121 ccagcagtta agatcacgtg ctgttctgcc agaggacctg ggttttattt cacgcatcta
     3181 tggtatatgg cccacacccg tctgtaactc cagttccagg gaatctgaca ctctctggtt
     3241 tgcacacagg taatgcacag acatacatgc aggcacaaca cccctaaata aacaaataaa
     3301 tataaaagta ac
//
