LOCUS       NM_001365338            1631 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus shieldin complex subunit 3 (Shld3), mRNA.
ACCESSION   NM_001365338
VERSION     NM_001365338.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Gupta,R., Somyajit,K., Narita,T., Maskey,E., Stanlie,A., Kremer,M.,
            Typas,D., Lammers,M., Mailand,N., Nussenzweig,A., Lukas,J. and
            Choudhary,C.
  TITLE     DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of
            NHEJ and PARP Inhibitor Sensitivity
  JOURNAL   Cell 173 (4), 972-988 (2018)
   PUBMED   29656893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154216.1.
            
            On Jan 14, 2022 this sequence version replaced NM_001365338.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2271742.1, AK012141.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               AC154216.1         74886-75231
            347-1631            AC154216.1         78341-79625
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13"
     gene            1..1631
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /note="shieldin complex subunit 3"
                     /db_xref="GeneID:113002583"
                     /db_xref="MGI:MGI:6194609"
     exon            1..346
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /inference="alignment:Splign:2.1.0"
     exon            347..1631
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             450..1217
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /note="REV7-interacting novel NHEJ regulator 1"
                     /codon_start=1
                     /product="shieldin complex subunit 3"
                     /protein_id="NP_001352267.1"
                     /db_xref="CCDS:CCDS88514.1"
                     /db_xref="GeneID:113002583"
                     /db_xref="MGI:MGI:6194609"
                     /translation="MDSCRMTTEVILHYRPYENDPKQLAKIAENVIQDFPTHPLPRFI
                     PWFPYDESKLPLKPERLPPVISEEAAESVKQYLAISEPGVKSQSYDCTVDLLEFQPSS
                     KLQHFIQSHTVKEQTNAAHLDKNSGKEKQHKQRSWSVSLASSHCPEKIFPLSRKLQAS
                     LRTLHLHSFHRARWTLEYSVCNNQTLEDIWTKLNRLIRRDELPSCNATIQRQLGQIWV
                     FCDIKCCEYVGNLLKERLSLIGKIDLFVHKYGVIFSM"
     regulatory      1281..1286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /note="hexamer: AATAAA"
     polyA_site      1301
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
     regulatory      1611..1616
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /note="hexamer: ATTAAA"
     polyA_site      1631
                     /gene="Shld3"
                     /gene_synonym="Rinn1"
                     /note="major polyA site"
ORIGIN      
        1 acccccaccg ggcaatgaaa gcttttggga agccgcgaat tgtcagcaag aagtggatcg
       61 ttgtagtcag ccttagtggt tgaggcaagt gtgtgcttct gagctaagga atggccatgg
      121 tacaaagatt tcactggggc cgtaaaggat ttagtgacaa gagaatttaa gtcctcacga
      181 gcagaaagaa gtcgggcgtg cgtttgaagc ccgatcttcc gggacctgtg ctttgcactt
      241 tccttccaga cccggtgaaa cttcagcctg cgtaggctga ggtcagaagt gcccgccaag
      301 atggaagtga atccccccaa acaggagcac ctgctggcgc taaaaggtcc tgtttcttct
      361 gatttgacaa gaaagacaat tttgtaataa taaattacat tctagctctg aagaattcag
      421 ctaagaaatt ttatcttcat taagagttaa tggatagctg cagaatgacc actgaagtaa
      481 tattacatta tcgaccatat gaaaacgatc ccaaacagct ggcaaaaatt gcagaaaatg
      541 taattcaaga ctttcctact cacccactac caagatttat tccttggttt ccatacgatg
      601 agtccaaact tccactcaag cctgaaagat taccaccagt aatttctgaa gaggctgctg
      661 agagcgtgaa gcagtactta gccatctcag aacctggtgt taaatcccag agctatgact
      721 gcacagtaga tctcttggag tttcagccta gctcaaaact gcagcacttt atccaatcac
      781 acacagtgaa ggagcagacc aatgcggcac atttggataa aaattcagga aaagaaaaac
      841 agcacaagca gagatcctgg agtgtttcgc ttgccagcag ccactgtcca gaaaagatct
      901 ttcctttgtc tagaaaattg caagctagtt taaggacact acatttgcac tcctttcata
      961 gagcaagatg gactttagaa tacagtgttt gcaacaacca gactctggaa gacatttgga
     1021 caaaactcaa tcgccttatc aggcgcgatg aactcccttc ttgtaacgct accatccaga
     1081 gacagttagg ccaaatatgg gtgttctgtg atattaagtg ctgtgaatat gtaggaaatc
     1141 tccttaaaga aagattatct cttattggga aaattgattt atttgtacac aaatatggtg
     1201 ttatttttag tatgtaataa attttgatca tcaggaaaag aattctgaat gaaatgaata
     1261 tggactgaaa ttgtgaaatg aataaatttt attgatcatt acatttttaa tgacttaaat
     1321 tttgatgctt ctttatagta tgaggaaata attgtgtaat atggatacag tttgtgtcca
     1381 gtatataaat atcacataat tccaaggctt gtcagtcaat tcaggaacat tccagattat
     1441 gtttattagc cttctgttaa gtgagctttg atgaattact gtttgtatta aaattgaacg
     1501 taagtgttct aacctttatc ccaatgattt gagtttaaat gaatgatttt attttagtga
     1561 atccttaaaa atccttattc atttaaatat aatatgagct gtgttgtaaa attaaaagtc
     1621 ttatcaaagt a
//
