LOCUS       NM_001360074            3411 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus shieldin complex subunit 2 (Shld2), transcript variant
            2, mRNA.
ACCESSION   NM_001360074 XM_006519647
VERSION     NM_001360074.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   King,A., Reichl,P.I., Metson,J.S., Parker,R., Munro,D.,
            Oliveira,C., Sommerova,L., Becker,J.R., Biggs,D., Preece,C.,
            Davies,B. and Chapman,J.R.
  TITLE     Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end
            joining reactions independently of 53BP1-governed repair pathway
            choice
  JOURNAL   Nat Struct Mol Biol (2024) In press
   PUBMED   39227718
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Gupta,R., Somyajit,K., Narita,T., Maskey,E., Stanlie,A., Kremer,M.,
            Typas,D., Lammers,M., Mailand,N., Nussenzweig,A., Lukas,J. and
            Choudhary,C.
  TITLE     DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of
            NHEJ and PARP Inhibitor Sensitivity
  JOURNAL   Cell 173 (4), 972-988 (2018)
   PUBMED   29656893
REFERENCE   3  (bases 1 to 3411)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   4  (bases 1 to 3411)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BF467362.1, BE648821.1, AK149442.1, BQ829235.1, AC154738.2,
            BE848901.1, BE630700.1 and BU756157.1.
            
            On Jan 26, 2018 this sequence version replaced XM_006519647.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861889.218928.1,
                                           SRR13948564.4972090.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849375,
                                           SAMN00849377 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BF467362.1         1-14
            15-94               BF467362.1         16-95
            95-563              BE648821.1         1-469
            564-2714            AK149442.1         480-2630
            2715-2733           BQ829235.1         567-585
            2734-2781           AC154738.2         26915-26962
            2782-3030           BE848901.1         1-249
            3031-3399           BE630700.1         1-369               c
            3400-3411           BU756157.1         1-12                c
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 20.8 cM"
     gene            1..3411
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /note="shieldin complex subunit 2"
                     /db_xref="GeneID:75698"
                     /db_xref="MGI:MGI:1922948"
     exon            1..351
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            352..406
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            407..1858
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1966
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2161
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2296
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2503
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2732
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2848
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     exon            2849..3402
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3380..3385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /note="hexamer: AATACA"
     polyA_site      3402
                     /gene="Shld2"
                     /gene_synonym="3110001K24Rik; Fam35a"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgcgg cccggaaggt cggtcccgtg tgcacgcgcg gcggcctggg aggtggaagc
       61 ggactgcagg gaggcctcgc tggctggcca gcagccccgg ccgcatgagt caccaggccc
      121 ctcctttccg ccttgggccg gaacggtgtg cggatccgct gcgcggccgg ccggagagtg
      181 acgccgcgcc gaagccgaag aggcgcgggg caaatcccga ctcatcccga atccgactgt
      241 tgaggaaccg ggaaagcaag aagcctcagg acaggaggga ggccctgacc ccacaggcct
      301 ttactttgca caaggtttta atttgttgta ggcaacttca gcgattttga gcaaaaaaga
      361 ttctccggcc tgcagcatga ttttggcttg atgtgaaaag attaagaaat tatgagtcaa
      421 ggatcacaag ttcacatttt tttgggtgct ccagttgctc cactgaaaac gacagtatca
      481 cagggaacag cttccttaat gtccactgcc aacgcctggg aaaaagtccg actcttctat
      541 aaacagcatt ccttgtatct gaaggctgga gatcaggagt ttaaaaatct ggaagactgt
      601 caagtcccca aaggtcttgg ccctccaggc cttcttagtg gtgacgtttt gacgacttct
      661 gtgaggaggt ctgctcaggt ggatgaggac tttaaacatt ctgcttccga agcacagagt
      721 gtgaaatccc aggttaacct gagtgatatg actagtggac aaatgtgtgg gcttggggac
      781 ggagttcagc atttacccga agaagaaaaa gatcaaaagc ttcaatgtaa aaataagaaa
      841 attacagatg agcagtctaa aaatcagtca gatccctgcg ttcggaactt ccaaagagat
      901 ttgtttgcgt tagaccttaa gtgtgcagcg aaactggatc tgggctgttg tactgaacag
      961 atgagtacag ggacaaagcc agagccgaca ggacatcgtg aaagacagag tcaggagagc
     1021 ttctctgaca cacggtgtga gccacagtcc gagggagcag tcaggaaggc ctcagaccag
     1081 agactgtctg ctgaagctga atttctcagc gtactgacct ccagccaacg ggcttttcta
     1141 gctcaaggga atgataaagg tcaggactgt ataaataaga gcactgtaaa tatggaggca
     1201 gaaccaacag gaagtcaggg agtcagacga actgaagggg attttagtaa gcctggtgga
     1261 gatttcgaag aagaatctga aaatgagcaa agccaagtgt attccctgga actttttagt
     1321 cctgtttgtc ctgaatcaga aagcagtcac agtcacataa accctggaaa aaatcttgaa
     1381 aacacaagct ctcaagaact tttcagtaat gaagaaaacc tgccaccaaa tgagttgtgt
     1441 agctcccatc caagtaccgc taacaggagc tggagctgta aagatgattc ccaccattct
     1501 aaagctcttt ctgaagtcca tcaggtgtcc aagaagccaa ggatggattc taatataaga
     1561 gaggcagcca aagcagtgcc ccagagggtc atgtctgaat taaaggacag taaaaaaata
     1621 tcattaataa aaaattgtga ttctaaaaat cagaagtata actgcttagt catggtgcta
     1681 accccatgtc atgtaaagga aataactata aaatctggac caaattctgg ttctaaagtg
     1741 ccattagcaa cgattgtagt tattgatcag tcagaaatta agaagagggt tgttctgtgg
     1801 aggactgcag catttggggc acttacagtg tttcttggcg atataatttt acttacagac
     1861 gttgttcttt atgaggatca gtggattggt gagacagtac tccaatcaac atttacaagt
     1921 cagttactaa atcttggaag ttattcatat gtccagcctg aaaaatattc caatgtaatt
     1981 gccaatgtta tacttcagga cttattgaca tatgtgtcta caaaacattc ctatctcaaa
     2041 gatctgcctc agaggcagcc tcagaaaatg aacactgtag agtttgtgga gttggagcag
     2101 ttgcagcctg atatattagt ccatgcagta ctcagagtag tggatgtcac tgtattgaca
     2161 gaggcattat atagttatag aggacagaag caaagaaaag ttgtgttaac cgtggaacag
     2221 gctcaaggtc aacactatgt gcttgtatta tggggtcctg gagcagcctg gtatactcag
     2281 cttcaaagga aaaaagattc tatttgggaa tttaagtacc tttttgttca gcggaacagc
     2341 atactagaaa acttagaatt acatacaaca ctgtggtctt cctgtgaatg cctatttgat
     2401 gatgacacaa gagcaatttc atttaaaaca aaatttcaaa aaaatacatc ctcctttgtc
     2461 aagatatcag acttagcgac tcacctagag gataaatatt caggagtggt tctgattaaa
     2521 gccaaggttt cagagctggt gttttctgct gcagcagctc agaagatagc tttgaatgct
     2581 cgcagcactc tgcagagcat cttctcctct cttcccagta ttgtgtatgc tggctgtgca
     2641 cactgtgggt cagaactaga gacagatgag aacaggatct acagacagtg tctgagctgc
     2701 ctgccatttg ttgggaaaaa aatattctat aggccggctt taatgaccat agttgatgga
     2761 agatataata cttgtgtcca tgttgggtca aagatgatgg agcagattct tctcaacatt
     2821 tctccagatt gcctcaacag agtaatagtg ccttcctcag aggtcaccta cggcatggtc
     2881 gcctcagacc tgctccattc cttgttggct gtcagtgctg agccttgtgt gctgaagatc
     2941 cagagccttt tcgagttgga tgagaacagc tacccactgc aacaggattt ctccctactc
     3001 gatttctgcc ctgactcacg taagctgtgg agcccaggac tttctctaag ggcagagggc
     3061 acaggaggca ttcctgggaa ggagtgatga aacaatctgt cttttgaaga tgcatttatt
     3121 tttattatat gtatgtaaat gttttgcctg tgtatgtctg tgtaccatgt atgtacctgg
     3181 cacccacaga ggccaggaga gggtgttaga tcccgagact agagtaatag atggttgtga
     3241 gccgccgtgt agattttgag aaaatgctct taactgctga gccatccaac cctatgatct
     3301 atcttttgaa atgaaatgac agggattttg cttagagttt ttaaaatagg taatttatta
     3361 gcataatata attgtgctaa atacataatt ttttaaagaa aaaaaaaaaa a
//
