LOCUS       NM_025858               1920 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus shisa family member 5 (Shisa5), transcript variant 1,
            mRNA.
ACCESSION   NM_025858
VERSION     NM_025858.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Sapir,T., Kshirsagar,A., Gorelik,A., Olender,T., Porat,Z.,
            Scheffer,I.E., Goldstein,D.B., Devinsky,O. and Reiner,O.
  TITLE     Heterogeneous nuclear ribonucleoprotein U (HNRNPU) safeguards the
            developing mouse cortex
  JOURNAL   Nat Commun 13 (1), 4209 (2022)
   PUBMED   35864088
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1920)
  AUTHORS   Gupta,R.K., Tripathi,R., Naidu,B.J., Srinivas,U.K. and
            Shashidhara,L.S.
  TITLE     Cell cycle regulation by the pro-apoptotic gene Scotin
  JOURNAL   Cell Cycle 7 (15), 2401-2408 (2008)
   PUBMED   18677103
  REMARK    GeneRIF: Mouse Scotin induces apoptosis and developmental defects
            when overexpressed in Drosophila.
REFERENCE   4  (bases 1 to 1920)
  AUTHORS   Zocchi,L., Bourdon,J.C., Codispoti,A., Knight,R., Lane,D.P.,
            Melino,G. and Terrinoni,A.
  TITLE     Scotin: A new p63 target gene expressed during epidermal
            differentiation
  JOURNAL   Biochem Biophys Res Commun 367 (2), 271-276 (2008)
   PUBMED   18164684
  REMARK    GeneRIF: We conclude that Scotin is a new p63 target gene induced
            during epithelial differentiation, a complex process that also
            involves ER stress induction.
REFERENCE   5  (bases 1 to 1920)
  AUTHORS   Draeby,I., Woods,Y.L., la Cour,J.M., Mollerup,J., Bourdon,J.C. and
            Berchtold,M.W.
  TITLE     The calcium binding protein ALG-2 binds and stabilizes Scotin, a
            p53-inducible gene product localized at the endoplasmic reticulum
            membrane
  JOURNAL   Arch Biochem Biophys 467 (1), 87-94 (2007)
   PUBMED   17889823
  REMARK    GeneRIF: ALG-2 binding to Scotin is strictly calcium dependent,
            indicating a role of this interaction in calcium signaling pathways
REFERENCE   6  (bases 1 to 1920)
  AUTHORS   Furushima,K., Yamamoto,A., Nagano,T., Shibata,M., Miyachi,H.,
            Abe,T., Ohshima,N., Kiyonari,H. and Aizawa,S.
  TITLE     Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings
  JOURNAL   Dev Biol 306 (2), 480-492 (2007)
   PUBMED   17481602
REFERENCE   7  (bases 1 to 1920)
  AUTHORS   Bourdon,J.C., Renzing,J., Robertson,P.L., Fernandes,K.N. and
            Lane,D.P.
  TITLE     Scotin, a novel p53-inducible proapoptotic protein located in the
            ER and the nuclear membrane
  JOURNAL   J Cell Biol 158 (2), 235-246 (2002)
   PUBMED   12135983
  REMARK    GeneRIF: Scotin plays a role in p53-dependent apoptosis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140383.2 and AK033545.1.
            
            On Sep 28, 2013 this sequence version replaced NM_025858.2.
            
            Transcript Variant: This variant (1) uses an alternate splice site
            in the coding region compared to variant 3. The resulting protein
            (isoform 1) is shorter but has the same N- and C-termini compared
            to isoform 3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033545.1, AF520699.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC140383.2         91541-91583         c
            44-1852             AK033545.1         4-1812
            1853-1920           AC140383.2         72358-72425         c
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.63 cM"
     gene            1..1920
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /note="shisa family member 5"
                     /db_xref="GeneID:66940"
                     /db_xref="MGI:MGI:1915044"
     exon            1..198
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..836
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; protein shisa-5; shisa homolog 5"
                     /codon_start=1
                     /product="protein shisa-5 isoform 1 precursor"
                     /protein_id="NP_080134.1"
                     /db_xref="CCDS:CCDS23543.1"
                     /db_xref="GeneID:66940"
                     /db_xref="MGI:MGI:1915044"
                     /translation="MAAPAPSLWTLLLLLLLLPPPPGAHGELCRPFGEDNSIPVFCPD
                     FCCGSCSNQYCCSDVLRKIQWNEEMCPEPESRFSTPAEETPEHLGSALKFRSSFDSDP
                     MSGFGATVAIGVTIFVVFIATIIICFTCSCCCLYKMCCPQRPVVTNTTTTTVVHAPYP
                     QPQPQPVAPSYPGPTYQGYHPMPPQPGMPAAPYPTQYPPPYLAQPTGPPPYHESLAGA
                     SQPPYNPTYMDSLKTIP"
     exon            199..355
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     exon            356..442
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     exon            443..558
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     exon            559..777
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     exon            778..1920
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1830..1835
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /note="hexamer: AATAAA"
     polyA_site      1847
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
                     /note="major polyA site"
     polyA_site      1896
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
     polyA_site      1905
                     /gene="Shisa5"
                     /gene_synonym="2310008D10Rik; 6430628I05Rik; mShisa5;
                     Scotin"
ORIGIN      
        1 ggcgggggtt ccggaaaccg gccggggcgg ggcaaggagg ctagggccgc gctggtcgcg
       61 gaggttgcgg cggcaccgtg gtcttgggct tggtccgtct gttcgtccgt ccgttggtct
      121 gtcccgccat ggctgcgccg gcgccctctc tgtggaccct attgctgctg ctgttgctgc
      181 tgccgccgcc tccgggtgcc catggtgagc tgtgcaggcc ctttggtgaa gacaattcga
      241 tcccagtgtt ctgtcctgat ttctgttgtg gttcctgttc caaccaatac tgctgctcgg
      301 acgtgctgag gaaaatccag tggaatgagg aaatgtgtcc tgagccagag tccagatttt
      361 ccacccccgc ggaggagaca cccgaacatc tgggttcagc gctgaaattt cgatccagtt
      421 ttgacagtga ccctatgtca gggttcggag cgaccgtcgc cattggcgtg accatctttg
      481 tggtgtttat tgccactatc atcatctgct tcacctgctc ctgctgctgt ctgtataaga
      541 tgtgctgccc ccaacgccct gtcgtgacca acaccacaac tactaccgtg gttcatgccc
      601 cttaccctca gcctcaacct caacctgtgg cccccagcta tcctggacca acataccagg
      661 gctaccatcc catgcccccc cagccaggaa tgccagcagc accctaccca acgcagtacc
      721 caccacccta cctggcccag cccacagggc cgccacccta ccatgagtcc ttggctggag
      781 ccagccagcc tccatacaac ccgacctaca tggattccct aaagacaatt ccctgaacct
      841 gcccccagcc tctttggctg ccatttatgt cgtgtgtgag tgagtgatac gcagagttct
      901 ttactgctgt ctgtggtgtg tgtgccttgt ctagacatgt ggcttcctct gctgttgacc
      961 aggtaggcgc aagtcttacc agtgtgggtc gggaccaacc tgttttcttc ctcacttgaa
     1021 attgtacttt ctgaaatttc aagcaaatta aaaacaataa ggtaggaggt atttcccacg
     1081 tcaccccaag gtgaccagcc atggcctgtc atacttagga gagcaagctt tttgcgggta
     1141 cagagcaggc tttggggggt aaccagctag ctgctgctag gcctttattc ccagggtttg
     1201 gctgcattgg cagtgaggca ggtggctggg ggtgacacca ggtgacaagg ggactcagtg
     1261 gcagggggtc acaccaggca gaacaccata cactctccat cagctgtctg tctggatgtc
     1321 actgtccttc ccggggctgt atagagggcc acatgtgttc actattcagg ctccactggg
     1381 ggaattttcc tacctttgct ggcttggctc ctgctcccag gccagggacc tcggtctgtc
     1441 tactacacac tctggtttct ccctgcactg tctttttact gttagccaaa cattttgcct
     1501 gttttctgtc tccagatgtg tgataattgg tgtgaggttg aaatccctgg ttcctggagg
     1561 acagacaacc tgacctccga ctgtcagttt cccttgacac catcttcata gaaatacctg
     1621 actcctgtac cacagtccag tttgtcccag tagcagggac accaaggcca atgggttatc
     1681 tggaccaaag gtggggtgga gggcctaggt ggtatctccg gcccagatgt gaatacctcc
     1741 atattccctg ttggttcctg tttcactggc tgttttagct ttgtgttgat tggtgtttct
     1801 gagcattcag actccgcacc ctcatttcta ataaatgcaa cattggaccc gcttcccctt
     1861 tcttcagcgc ctaggcagct ggccttggct ctacaccaaa aactatttct gtctacttct
//
