LOCUS       NM_175687               1683 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus shiftless antiviral inhibitor of ribosomal
            frameshifting (Shfl), mRNA.
ACCESSION   NM_175687
VERSION     NM_175687.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Hanners,N.W., Mar,K.B., Boys,I.N., Eitson,J.L., De La
            Cruz-Rivera,P.C., Richardson,R.B., Fan,W., Wight-Carter,M. and
            Schoggins,J.W.
  TITLE     Shiftless inhibits flavivirus replication in vitro and is
            neuroprotective in a mouse model of Zika virus pathogenesis
  JOURNAL   Proc Natl Acad Sci U S A 118 (49) (2021)
   PUBMED   34873063
  REMARK    GeneRIF: Shiftless inhibits flavivirus replication in vitro and is
            neuroprotective in a mouse model of Zika virus pathogenesis.
REFERENCE   3  (bases 1 to 1683)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 1683)
  AUTHORS   Pereira,J.D., Sansom,S.N., Smith,J., Dobenecker,M.W.,
            Tarakhovsky,A. and Livesey,F.J.
  TITLE     Ezh2, the histone methyltransferase of PRC2, regulates the balance
            between self-renewal and differentiation in the cerebral cortex
  JOURNAL   Proc Natl Acad Sci U S A 107 (36), 15957-15962 (2010)
   PUBMED   20798045
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC170598.2, AK149161.1, BY625900.1 and AK034516.1.
            
            On Nov 15, 2007 this sequence version replaced NM_175687.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043448.1, AK149161.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AC170598.2         96225-96247
            24-1468             AK149161.1         4-1448
            1469-1675           BY625900.1         180-386
            1676-1683           AK034516.1         3125-3132
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.65 cM"
     gene            1..1683
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="shiftless antiviral inhibitor of ribosomal
                     frameshifting"
                     /db_xref="GeneID:319278"
                     /db_xref="MGI:MGI:2441788"
     exon            1..295
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     CDS             275..1147
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="UPF0515 protein C19orf66 homolog; repressor of
                     yield of DENV protein homolog"
                     /codon_start=1
                     /product="shiftless antiviral inhibitor of ribosomal
                     frameshifting protein homolog"
                     /protein_id="NP_783618.1"
                     /db_xref="CCDS:CCDS22885.1"
                     /db_xref="GeneID:319278"
                     /db_xref="MGI:MGI:2441788"
                     /translation="MAQDGVELEKSVRRLREKFHGKVSPKKAGALMRKFGSDHTGVGR
                     SIVYGVKQKDGQELSNDLDAQDPPEDMKQDQDIQAVATSLLPLTQANLRMFQRAQDDL
                     IPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPLDKMWGLAEFHCPKCRH
                     NFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDLDRRSTHTHSCSAADCYNRREPHV
                     PGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLVDDLDHLILEDLKEEE
                     EEEEEEEEDGGPRE"
     exon            296..419
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            420..469
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            470..508
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            509..658
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            659..762
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            763..917
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1683
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1546..1551
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="hexamer: AATAAA"
     polyA_site      1565
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="major polyA site"
     regulatory      1651..1656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="hexamer: AATAAA"
     polyA_site      1672
                     /gene="Shfl"
                     /gene_synonym="A230050P20Rik; RyDEN"
                     /note="major polyA site"
ORIGIN      
        1 cgcgcagcgt ggggacatgt gaccgactgc agaggccgcg ctgctgcgca ggacgcggtg
       61 aggcgcgctc ggaggcccgg gagcaccacc cgggagcact acagggcgcg tgagcagacg
      121 cgtggccgca aggcctgcgc ggggtctcct cagggtcccc agctttacgt agaaacgaaa
      181 ctgaaagagg ggcccgaggg aggcggagct gcggcgacgc cacccaggct tgcgaggccc
      241 ggacccaggc gcagccaggt gtgctccggc caccatggct caggatggcg tggagttgga
      301 gaagagcgtc aggcgcctcc gggagaagtt tcatgggaaa gtgtccccca agaaggcagg
      361 ggctcttatg aggaagtttg gcagcgacca caccggagtt gggcgctcta tcgtgtatgg
      421 tgtcaagcag aaagacggac aggagctgag caacgacctg gacgctcagg acccaccaga
      481 ggacatgaag caagaccaag atatccaggc agtagccacc tctctgttgc ccctgacgca
      541 agccaatctt cgaatgttcc aaagagccca agatgacctc atccctgccg tggaccgaca
      601 gtttgcctgc tcctcctgtg accatgtatg gtggcgccgt gtaccccaga ggaaagaggt
      661 gtcacggtgt cgcaagtgta ggaagcgtta cgaaccagtg ccacttgaca agatgtgggg
      721 cctggcagag ttccactgcc caaagtgtcg ccacaacttc cggggctggg cacagatggg
      781 gtccccatca ccctgctacg ggtgcggctt ccctgtgtac cccacccgga tcctgccccc
      841 acgctgggac cgtgacctgg accgtcgcag cacacacacc cactcctgct cagctgcaga
      901 ttgctacaat cggagagagc cccatgtgcc tgggacctcc tgcgcccacc ccaagagccg
      961 taagcagaac cacctcccca aagtgctgca cccgagcaac ccccacatca gcagcggctc
     1021 cacggtggcc acgtgcctga gccaaggagg cctcgtggat gacctggacc acctcatcct
     1081 ggaggacctg aaagaggagg aggaggagga ggaggaggaa gaggaggacg gtgggccccg
     1141 agagtgaccc tggctaggtg cacacaggaa gacagacaca gtgtgtggcc acctcgtgtt
     1201 gctataagat accagctgaa ggagatagga acacccttgg gggtactgat gggacagtca
     1261 caagcgcaca agccgggtca acataacagc tttcttcaga gagtacgccc caccacggaa
     1321 ctcggcccag tagaccccgt cctggtatcg gctccggtaa tgacctccat gataccatac
     1381 tccattgagg ttagagtggg cacaggcatg gtaccaccag cccccacgat ggtacagggc
     1441 acagttacct gagggagagg gagaggggga gagagagaga gagagagaga gagagagaga
     1501 gagagagaga gagagagaga gagagagttc gatttttttc accaaaataa aagtctctac
     1561 cagcacttct ttgtgcaaag cttttgcaag cattcctcaa ccttccctat cttattgaat
     1621 agaagccctg ctactccatc tcttcaccga aataaaaggc tctggcttct cactttcttc
     1681 ttc
//
