LOCUS       NM_001113373            8238 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Mus musculus SH3 and multiple ankyrin repeat domains 2 (Shank2),
            transcript variant 2, mRNA.
ACCESSION   NM_001113373
VERSION     NM_001113373.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ratsika,A., Codagnone,M.G., Bastiaanssen,T.F.S., Hoffmann
            Sarda,F.A., Lynch,C.M.K., Ventura-Silva,A.P., Rosell-Cardona,C.,
            Caputi,V., Stanton,C., Fulling,C., Clarke,G. and Cryan,J.F.
  TITLE     Maternal high-fat diet-induced microbiota changes are associated
            with alterations in embryonic brain metabolites and adolescent
            behaviour
  JOURNAL   Brain Behav Immun 121, 317-330 (2024)
   PUBMED   39032541
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Lai,W., Zhao,Y., Chen,Y., Dai,Z., Chen,R., Niu,Y., Chen,X.,
            Chen,S., Huang,G., Shan,Z., Zheng,J., Hu,Y., Chen,Q., Gong,S.,
            Kang,S., Guo,H., Ma,X., Song,Y., Xia,K., Wang,J., Zhou,L., So,K.F.,
            Wang,K., Qiu,S., Zhang,L., Chen,J. and Shi,L.
  TITLE     Autism patient-derived SHANK2BY29X mutation affects the development
            of ALDH1A1 negative dopamine neuron
  JOURNAL   Mol Psychiatry 29 (10), 3180-3194 (2024)
   PUBMED   38704506
  REMARK    GeneRIF: Autism patient-derived SHANK2B[Y29X] mutation affects the
            development of ALDH1A1 negative dopamine neuron.
REFERENCE   3  (bases 1 to 8238)
  AUTHORS   Kreutzmann,J.C., Kahl,E. and Fendt,M.
  TITLE     Sex-specific modulation of safety learning in Shank2-deficient mice
  JOURNAL   Prog Neuropsychopharmacol Biol Psychiatry 132, 110973 (2024)
   PUBMED   38369099
  REMARK    GeneRIF: Sex-specific modulation of safety learning in
            Shank2-deficient mice.
REFERENCE   4  (bases 1 to 8238)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 8238)
  AUTHORS   Kim,J., Jung,M.W. and Lee,D.
  TITLE     Reward learning improves social signal processing in autism model
            mice
  JOURNAL   Cell Rep 42 (10), 113228 (2023)
   PUBMED   37815916
REFERENCE   6  (bases 1 to 8238)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   7  (bases 1 to 8238)
  AUTHORS   Uemura,T., Mori,H. and Mishina,M.
  TITLE     Direct interaction of GluRdelta2 with Shank scaffold proteins in
            cerebellar Purkinje cells
  JOURNAL   Mol Cell Neurosci 26 (2), 330-341 (2004)
   PUBMED   15207857
REFERENCE   8  (bases 1 to 8238)
  AUTHORS   Kim,J.Y., Han,W., Namkung,W., Lee,J.H., Kim,K.H., Shin,H., Kim,E.
            and Lee,M.G.
  TITLE     Inhibitory regulation of cystic fibrosis transmembrane conductance
            regulator anion-transporting activities by Shank2
  JOURNAL   J Biol Chem 279 (11), 10389-10396 (2004)
   PUBMED   14679199
  REMARK    GeneRIF: Shank2 negatively regulates CFTR and this may play a
            significant role in maintaining epithelial homeostasis
REFERENCE   9  (bases 1 to 8238)
  AUTHORS   Bolis,A., Corbetta,S., Cioce,A. and de Curtis,I.
  TITLE     Differential distribution of Rac1 and Rac3 GTPases in the
            developing mouse brain: implications for a role of Rac3 in Purkinje
            cell differentiation
  JOURNAL   Eur J Neurosci 18 (9), 2417-2424 (2003)
   PUBMED   14622142
REFERENCE   10 (bases 1 to 8238)
  AUTHORS   Du,Y., Weed,S.A., Xiong,W.C., Marshall,T.D. and Parsons,J.T.
  TITLE     Identification of a novel cortactin SH3 domain-binding protein and
            its localization to growth cones of cultured neurons
  JOURNAL   Mol Cell Biol 18 (10), 5838-5851 (1998)
   PUBMED   9742101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC152166.4, AC124520.4 and AC140268.2.
            
            On Jan 18, 2019 this sequence version replaced NM_001113373.2.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (b).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131,
                              SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-497               AC152166.4         186709-187205
            498-816             AC152166.4         191004-191322
            817-986             AC152166.4         198069-198238
            987-1100            AC152166.4         203666-203779
            1101-1176           AC124520.4         120872-120947       c
            1177-1259           AC124520.4         94875-94957         c
            1260-1384           AC124520.4         93246-93370         c
            1385-1520           AC140268.2         151888-152023       c
            1521-1601           AC140268.2         151055-151135       c
            1602-1610           AC140268.2         150840-150848       c
            1611-1631           AC140268.2         149225-149245       c
            1632-1762           AC140268.2         142026-142156       c
            1763-1874           AC140268.2         140254-140365       c
            1875-1895           AC140268.2         139410-139430       c
            1896-4302           AC140268.2         135618-138024       c
            4303-8238           AC140268.2         122992-126927       c
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 88.45 cM"
     gene            1..8238
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="SH3 and multiple ankyrin repeat domains 2"
                     /db_xref="GeneID:210274"
                     /db_xref="MGI:MGI:2671987"
     exon            1..497
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             461..4879
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="isoform b is encoded by transcript variant 2; SH3
                     and multiple ankyrin repeat domains protein 2; cortBP1;
                     cortactin-binding protein 1; SH3/ankyrin domain gene 2"
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein
                     2 isoform b"
                     /protein_id="NP_001106844.3"
                     /db_xref="CCDS:CCDS85484.1"
                     /db_xref="GeneID:210274"
                     /db_xref="MGI:MGI:2671987"
                     /translation="MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSD
                     NNLNAGAPEWAVCSAATSHRSLSPQLLQQTPSKPDGATKSLGSYTPGPRSRSPSLNRL
                     GGTAEDGKRTQPHWHVGSPFTPGANKDSLSTFEYPGPRRKLYSAVPGRLFVAVKPYQP
                     QVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRAD
                     RSKKLFRHYTVGSYDSFDAASDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTP
                     TPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLI
                     LKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELVDKASVRKKKDK
                     PEEIVPASKPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVP
                     GSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDI
                     PPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVVAKVPPATRSDTVATM
                     MREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYV
                     PAKPARRKGVLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAT
                     EPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAP
                     RMQASKFPEEGGFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTS
                     KAKGPESGPAAPLKSSSPAGPENYVHPLTGRLLDPSSPLALALSARDRAMQESQQGHK
                     GEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLGKDRRAD
                     DKKNMLINIVDTAQQKSAGLLMVHTVDVPMAGPPLEEEEDREDGDTKPDHSPSTVPEG
                     VPKTEGALQISAAPEPAVAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTE
                     PLPPPLEFANSFDIPDDRAASVPALADLVKQKKNDTSQPPTLNSSQPANSTDSKKPAG
                     ISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGE
                     SMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPP
                     PPPGSAQAGVAKVIQPRTSKLWGDVPEVKSPILSGPKANVISELNSILQQMNRGKSVK
                     PGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYES
                     RTSGPRRAPSPVVSPTELSKEILPTPPPPSATAASPSPTLSDVFSLPSQSPAGDLFGL
                     NPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKETFMDNEID
                     GSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR"
     exon            498..816
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            817..986
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1100
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1176
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1259
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1384
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1520
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1601
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1610
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1631
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1762
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1874
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1895
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..4302
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4303..8238
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4976
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="major polyA site"
     regulatory      7765..7770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="hexamer: ATTAAA"
     polyA_site      7804
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="major polyA site"
     regulatory      8217..8222
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="hexamer: AATAAA"
     regulatory      8221..8226
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="hexamer: AATATA"
     polyA_site      8222
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="major polyA site"
     polyA_site      8235
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
     polyA_site      8238
                     /gene="Shank2"
                     /gene_synonym="mKIAA1022; ProSAP1"
                     /note="major polyA site"
ORIGIN      
        1 acaggcgcac ggagcgccca gcagcagccg ggtttccttg gataactggg gctccggctc
       61 acaaatgcct cccggtgtct cctggccgac agctcgcgcg caatcgcctg ctccatgtta
      121 accctgcgtg gtgctgcctc tccgatgccc tgcttgctct ctcggcagcg tggcactgct
      181 cacatgagga tcaccgccag aggtagccgg ttctgagagc agcctcagtc tgcgcacagt
      241 tgcttcccag ctgacagaca gaatatgggg tgggctttgt caccagcacg ctgggtctgg
      301 catgcccagc gatgctcggg gctgcagcgc gcccagacgc ccgccactgc tcgccgggtc
      361 ccagccgcgc cagctccgag gggccgggct gcgcgcctag ccttcctgcc cgccgagagg
      421 gttatataat gagcggaccg ctgccaaagt cacagggaaa atgaagtctt tgttaaatgc
      481 cttcaccaag aaggaagtgc ccttcagaga ggccccagct tattccaacc gcaggcggcg
      541 gccccccaac acattggctg cccccagagt tcttctgcgt tctaatagtg acaacaacct
      601 caatgctggt gcacctgaat gggctgtctg ctctgcagcc acctcccacc gaagcctctc
      661 accccaactg ctgcagcaga cacccagcaa acctgatgga gccaccaaga gccttggaag
      721 ctatacccct gggccccgca gccgttcccc ttcactcaac aggctgggtg gtactgctga
      781 ggatggcaag aggacacagc cacattggca tgtggggtcg cccttcactc ctggtgccaa
      841 caaggactcc ctctcgacct ttgagtaccc agggcccagg aggaagctat atagtgcggt
      901 gcctgggaga ctcttcgtcg ccgtcaagcc ataccaaccc caagtcgacg gcgagatccc
      961 ccttcaccga ggcgacaggg tcaaagttct gagcatcgga gagggcggct tctgggaagg
     1021 cagtgcccgt ggccacatcg ggtggttccc agctgagtgt gtggaagagg tgcagtgtaa
     1081 accccgggac agccaggcag aaacccgcgc ggaccgcagc aagaagctct tccggcatta
     1141 cacggtgggc tcctatgaca gctttgatgc tgccagtgac tgcattattg aggacaagac
     1201 ggtggtcctg caaaagaagg acaacgaggg ctttggattt gtgctccgag gggcaaaagc
     1261 cgataccccc attgaggaat tcacacccac gccagcattt ccagccctgc agtacctgga
     1321 gtccgtggat gaaggtgggg tggcatggca agccggacta aggaccgggg acttcttgat
     1381 tgaggttaac aatgaaaatg tcgtcaaggt gggccacagg caggtggtga acatgatccg
     1441 ccagggaggg aatcacctca tccttaaggt cgtcacggtg accaggaatc tagaccctga
     1501 tgatacagcc agaaagaaag ctcccccacc tccaaagcgg gctccgacca cggccctcac
     1561 cctgcgttcc aagtccatga cagcggagtt ggaggaactc gtggacaaag cctcagtccg
     1621 gaagaagaag gataaaccgg aagagatagt cccagcctcc aagccctcca ggactgcaga
     1681 gaacgtggcc atcgaatcca gggtggcgac catcaagcag cggcccacaa gccggtgctt
     1741 cccagctgcc tctgatgtga attccgtgta cgagcgccaa gggattgctg taatgacgcc
     1801 cacggtccct gggagcccga aaggcccatt tctgggcctc cctcgaggta cgatgcgaag
     1861 gcagaaatcg atagacagca gaatctttct atcagggata acagaggaag agcggcagtt
     1921 tctggctccc ccaatgctga agttcacccg aagcttgtcc atgccagaca cttctgagga
     1981 catcccccct ccgccacagt ctgtgccccc ctctccccct cctccttccc ccaccacata
     2041 caactgtccc aggtccccga ctccaagagt ctatgggaca attaagcccg cgttcaatca
     2101 gaaccccgtc gtcgccaagg tgcccccagc caccaggtct gacactgtgg ccaccatgat
     2161 gcgggaaaag gggatgttct acaggagaga gctggacaga ttttccctgg actcagaaga
     2221 cgtctacagc cgcagccccg ccccacaggc cgccttccgc accaagcggg gacagatgcc
     2281 tgagaacccg tactcagagg tgggaaagat agccagcaag gccgtctatg tccctgccaa
     2341 gccagccagg cggaagggcg tgctggtaaa gcagtccaac gtggaggata gccccgagaa
     2401 gacgtgctcc atacccatcc caaccatcat cgtcaaggaa ccctccacca gcagcagcgg
     2461 caagagcagc caggggagca gcatggagat tgacccccag gccaccgagc ccggccagct
     2521 gcggccagat gacagcctca ccgtcagcag cccctttgct gcggccatcg ctggggctgt
     2581 gcgtgaccgg gagaagcgtc tggaagccag gaggaattcc ccagccttcc tctccaccga
     2641 cctgggagat gaggacgtgg gtctggggcc gcctgctccc cggatgcagg cctccaagtt
     2701 cccagaggag ggtgggtttg gtgacgagga tgagacggaa caaccgctgt tgcctacccc
     2761 cggggcagca cccagggagc tggagaatca cttcctaggt ggtggtgagg ctggtgctca
     2821 gggggaggct gggggacccc tgagttccac atccaaagcc aaggggcctg agagtggccc
     2881 agcagccccc ctcaagagca gcagcccagc cggccctgag aattacgtgc acccactcac
     2941 agggcggctg cttgacccca gctccccact ggccctggca ctctcagcca gggaccgagc
     3001 catgcaggag tcccagcagg ggcacaaagg agaggccccc aaggctgacc ttaacaagcc
     3061 tctctacatc gataccaaaa tgcggcccag cgtggagtcc ggctttcctc cagtcaccag
     3121 acagaacacc aggggtcccc tgcgacggca agagacagag aacaagtacg agacagacct
     3181 gggcaaggac cggagggcgg acgacaagaa gaacatgctg atcaacatcg tggacactgc
     3241 ccagcagaag tcagctggcc tactgatggt gcacacagtg gacgtcccca tggccgggcc
     3301 acccctggag gaagaggagg acagagagga tggggataca aagccagacc actcaccctc
     3361 cacagtgcca gaaggcgttc ccaaaaccga aggtgcttta cagatctccg ctgccccgga
     3421 gcccgccgtc gcgcccggca ggaccatcgt ggcggcgggc tccgtggaag aggcggtgat
     3481 tctgccattc cgcatccccc ctccccctct ggcatccgtg gacttggatg aggactttct
     3541 tttcacagaa ccgttgcctc cccccctgga attcgccaat agttttgata tccccgatga
     3601 ccgggcagct tcagttcccg ctctggctga cctggtcaag cagaagaaaa acgacacctc
     3661 tcagccccct acgttgaact ccagccaacc agccaactcc acagacagta agaagccagc
     3721 cggtatctcg aactgtctgc cctcctcgtt cctgccaccc cccgaaagtt tcgatgcagt
     3781 caccgactcg gggattgagg aggtggacag ccggagtagc agcgaccacc acctggagac
     3841 taccagcacc atctccacgg tgtccagcat ctccacgctg tcctcagagg gcggcgagag
     3901 catggacacc tgcacagtct atgcagacgg gcaagccttt gtggttgaca agcccccagt
     3961 acctccaaag ccaaaaatga agcccatcgt tcacaagagc aacgcacttt accaagacac
     4021 gctcccagaa gaggacacgg atggctttgt gatcccccca cctgcacccc cgcccccgcc
     4081 gggcagcgcc caggccggtg tggcgaaggt catccagcca aggacctcca agttgtgggg
     4141 tgacgttcca gaggtcaaaa gcccaattct ctcaggccca aaggcaaatg tcattagtga
     4201 gctaaactcc attctgcagc agatgaacag ggggaaatcg gtcaagcccg gggaagggct
     4261 ggagctgccg gtgggagcca agtcggccaa cctcgctcca agaagcccgg aggtcatgag
     4321 caccgtctca ggtacacgga gcacgacggt caccttcact gtccgccctg gcacctccca
     4381 gcccatcacc ctgcagagcc ggccccccga ctatgaaagc agaacctcag gacctagacg
     4441 cgccccaagc cctgtggttt caccaacgga attgagcaaa gagatcctgc ccacccctcc
     4501 ccctccgtcc gccactgcag cctctccctc ccccacactc tcagatgtct ttagccttcc
     4561 gagccagtcc cctgcagggg acctctttgg cttgaaccca gcaggacgga gcaggtcacc
     4621 atctccttca atattgcaac agccaatctc aaataagcct tttacaacta agcctgtcca
     4681 cctgtggacg aaaccagatg tggcagactg gctggaaagt ctgaacttgg gtgaacacaa
     4741 ggagacgttc atggacaatg agattgacgg cagccacctg ccaaaccttc agaaggaaga
     4801 cttgatagat cttggggtga ctcgagttgg gcataggatg aacatagaaa gggctttgaa
     4861 acagctgctg gacagataag ggtggctgcc ctgggccttc acagactcct ttcttataag
     4921 tagagatggg cttatgttga aatgtgtggt ggccaagcag aggctgacag caccaacctc
     4981 cagtctgtgg gtttgtctct gggtacccaa agatgtgctg tgggtgtccc ttggtgtggc
     5041 caaactgggg ccacagatat cttgggtggc ttctgtccaa agggacagta agggggtttc
     5101 tgtcctggtc tcagaggctg ccatagacgg tatgtgggac cctctcctcc tgtggcagac
     5161 accagtgtac tccagatacc tcctgaggcc tccctcctct gccttctggg caactctcac
     5221 ccctcagccc tggaacacag cctttcttca ggcctttggc ctctgggtgg gctcattggc
     5281 tctgtccctc ccctgtgctt ggctctcagt gagcagttcc aggtggagcc ctgcctgagc
     5341 cagactgtag agaacactgt cccatccacc tctttgtccg actcctgtga cgtgcatcgg
     5401 agcttctttg gcccctaggt ccttggcagt ggtggctgtg gacaggatcg tgccctgctt
     5461 gcttaacttg ctgtctttat ttctgcaaac agattagcat aattccaggg tggccaaaat
     5521 gaagtcacac ggagttagtc aaagcacaaa gtcacgatcc tgaggaggag ggaggcctgg
     5581 ctgtagagcc agccaatgtg tggctgccca agcccacagc ctcctccagg gcaaggacag
     5641 gtgtctgccc agagcatctg cccaggaggg agaggttggg gctcttgaaa tttactctaa
     5701 ttggatgtct gcagagctcc acagaggtca gaacaggtga gcagctactt ttacaaggac
     5761 aagaggacct gaggcttgtg gtaagcatgg ctgccatcgg gaagctgctg tcctccctgg
     5821 tagcagatgg aatgccatac ccttcatctg caccaggcct tggctgagca tcctgccgag
     5881 gcagcccttt ggctctcaga ggccaagaaa ggtggaactc aggggccttt ctcccttctc
     5941 tcacaaagtg gggatcccac cagcttgtct taacatctgg ctctcagaga agcccgtggt
     6001 caggacctgg tggaggacag aaagtggggc acaggctgtg gcaaagttta ggtgtgtgtg
     6061 gaaaagaagg tggggagcat cgtggaccaa gcctcacctc aggcccagcc acctgagccc
     6121 acccttctcg tccccagatg tctccctccc aaaacaatac ctctaatgtc ccagggaggt
     6181 cactgactgc acacagccac catgcagcca gccaggctcc cgaaaccccc tctcccccac
     6241 gctcagagga gactcgaatg tcaggtgacg gttttatcat ttcagaacta gcccagccca
     6301 tctctaatta taaaacatct ttttcctttt tttttttttc tctcttcact tatgctcaca
     6361 accacgtgtc tgacgaggcc tggaacagcg ctacaatgaa cacataaaat ttagcaatta
     6421 aaaaaaatct ttctttactg caagtttaaa tatgtgtaca gatagttcat aagcacaata
     6481 ctttaagaag aaaatggctg gtctactagg cagcctctgt gccacccagt gctggaaaac
     6541 taaagggaaa gggggttgtg gtttaaaaat accatttcct tgggacaatt atacaagcga
     6601 cctttaaaaa attaaccttc cctggtcaca tccgaacctg tgacatcaag ttgtttttca
     6661 gcttttgcca cagtttcatc attgaagggc aattatttca tcaaaaagat tagaacccat
     6721 gactctgcgg tttctccaac aacaatgcac atacatgcat actacacaca cacacacaca
     6781 cacacacaca cacacacaca cacacaccct catgcatcca ccacaaccct tgttatggag
     6841 accagacctt tgaatcttat tctgtcccca aaaccctttg caggtcaacc ctaacttctg
     6901 agtcatccag tcagctggta ccaggtgact gttgagcacc acgctctggg ttccctcatc
     6961 cacagcatgg cgaatgaatg agtaacactc cagagatgct gcaagtccag acactgcggg
     7021 tagccactga agaattcaga gagtgtgaaa atgtggcctc gccattaact tgtcttccaa
     7081 tgggtggttc ttcgaagtcc cctcagtgtg ccatagcact aggctccttt cctttgcttc
     7141 tggttggcat tacctctgtg ctcagtgcca ggcagatgct aggcaggaga gacctggtgg
     7201 cttggggcac gctgagctta ggcagcaggt gacaagctgc gactgccttc cttagacttc
     7261 aagccacatg cctccaagca gaagcaagct gactgtcagc cttggaagct gataggccag
     7321 ctttgctttg cagtgtctgg tcacaggaac acagctggac ctatcagaac tgacccccaa
     7381 tcacccaaac acacatttgc ctagatactg ggtctctgtc tctgacagtt gattttgcgc
     7441 agaaagcaat agtccctcat tgaaattgcc gcccattgtt cccccgacac acactcggac
     7501 atgtcaggac caggccctac cccaggaccc tcttcagtgg tgaaccagat gttaaactgc
     7561 tcatgaggac catgttacca ctattccagg ttttaagatc aacttttgtt atatactgta
     7621 atttaaataa ctgcataaat ttacatgcct agtttctgta atattgtgta tacaaaacca
     7681 aaatctctca agctgtaaat tgtgtatacc catcaggatc cctactctgg gacatccgtg
     7741 cgcattttag atttaattca tacaattaaa aactcaatgt gactgctgac gtgctgaaac
     7801 tttacataag aattatttgt gatactttag ttaataaaat ggtgaggttt tttttttttt
     7861 tcctgagtta ttgaacaggc aaacattacc cagctgacct ggcaatgacc ttttgtgtgt
     7921 gtgcgggtag gggggccaca aatattgatt ttttttctcc cattagcaat tttgggtttt
     7981 gaatatgaac atgtttcaca ctatagtttg tgtgtaacga cacatgtcac attacctttg
     8041 ttgctgttaa ttttgtgctc cttttctttt tagactttat ttatttagaa ggagagagag
     8101 gaagagggag agtgagggag agtgagaaag tgagagagag agagagttcc tgtactttgc
     8161 acttttcccc caaatccttc aatctcttgt atggcaacca aaattactgt aaaaaaaata
     8221 aatatactct tgcaataa
//
