LOCUS       NM_008018              10495 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus SH3 and PX domains 2A (Sh3pxd2a), transcript variant
            1, mRNA.
ACCESSION   NM_008018 XM_916341
VERSION     NM_008018.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Martinez-Mayer,J., Brinkmeier,M.L., O'Connell,S.P., Ukagwu,A.,
            Marti,M.A., Miras,M., Forclaz,M.V., Benzrihen,M.G., Cheung,L.Y.M.,
            Camper,S.A., Ellsworth,B.S., Raetzman,L.T., Perez-Millan,M.I. and
            Davis,S.W.
  TITLE     Knockout mice with pituitary malformations help identify human
            cases of hypopituitarism
  JOURNAL   Genome Med 16 (1), 75 (2024)
   PUBMED   38822427
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 10495)
  AUTHORS   Barbayianni,I., Kanellopoulou,P., Fanidis,D., Nastos,D.,
            Ntouskou,E.D., Galaris,A., Harokopos,V., Hatzis,P., Tsitoura,E.,
            Homer,R., Kaminski,N., Antoniou,K.M., Crestani,B., Tzouvelekis,A.
            and Aidinis,V.
  TITLE     SRC and TKS5 mediated podosome formation in fibroblasts promotes
            extracellular matrix invasion and pulmonary fibrosis
  JOURNAL   Nat Commun 14 (1), 5882 (2023)
   PUBMED   37735172
  REMARK    GeneRIF: SRC and TKS5 mediated podosome formation in fibroblasts
            promotes extracellular matrix invasion and pulmonary fibrosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10495)
  AUTHORS   Pezinski,M., Maliszewska-Olejniczak,K., Daszczuk,P., Mazurek,P.,
            Niewiadomski,P. and Redowicz,M.J.
  TITLE     Tks5 Regulates Synaptic Podosome Formation and Stabilization of the
            Postsynaptic Machinery at the Neuromuscular Junction
  JOURNAL   Int J Mol Sci 22 (21), 12051 (2021)
   PUBMED   34769479
  REMARK    GeneRIF: Tks5 Regulates Synaptic Podosome Formation and
            Stabilization of the Postsynaptic Machinery at the Neuromuscular
            Junction.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10495)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10495)
  AUTHORS   Rufer,A.C., Rumpf,J., von Holleben,M., Beer,S., Rittinger,K. and
            Groemping,Y.
  TITLE     Isoform-selective interaction of the adaptor protein Tks5/FISH with
            Sos1 and dynamins
  JOURNAL   J Mol Biol 390 (5), 939-950 (2009)
   PUBMED   19464300
  REMARK    GeneRIF: The tandem SH3A and SH3B domains of Tks5 constitute a
            versatile module for the implementation of isoform-specific
            protein-protein interactions.
REFERENCE   7  (bases 1 to 10495)
  AUTHORS   Thompson,O., Kleino,I., Crimaldi,L., Gimona,M., Saksela,K. and
            Winder,S.J.
  TITLE     Dystroglycan, Tks5 and Src mediated assembly of podosomes in
            myoblasts
  JOURNAL   PLoS One 3 (11), e3638 (2008)
   PUBMED   18982058
  REMARK    GeneRIF: Data suggest that Src-dependent phosphorylation of
            beta-dystroglycan results in the formation of a Src/dystroglycan
            complex that drives the SH3-mediated association between
            dystroglycan and Tks5 which together regulate podosome formation in
            myoblasts.
REFERENCE   8  (bases 1 to 10495)
  AUTHORS   Munton,R.P., Tweedie-Cullen,R., Livingstone-Zatchej,M.,
            Weinandy,F., Waidelich,M., Longo,D., Gehrig,P., Potthast,F.,
            Rutishauser,D., Gerrits,B., Panse,C., Schlapbach,R. and Mansuy,I.M.
  TITLE     Qualitative and quantitative analyses of protein phosphorylation in
            naive and stimulated mouse synaptosomal preparations
  JOURNAL   Mol Cell Proteomics 6 (2), 283-293 (2007)
   PUBMED   17114649
REFERENCE   9  (bases 1 to 10495)
  AUTHORS   Abram,C.L., Seals,D.F., Pass,I., Salinsky,D., Maurer,L., Roth,T.M.
            and Courtneidge,S.A.
  TITLE     The adaptor protein fish associates with members of the ADAMs
            family and localizes to podosomes of Src-transformed cells
  JOURNAL   J Biol Chem 278 (19), 16844-16851 (2003)
   PUBMED   12615925
REFERENCE   10 (bases 1 to 10495)
  AUTHORS   Lock,P., Abram,C.L., Gibson,T. and Courtneidge,S.A.
  TITLE     A new method for isolating tyrosine kinase substrates used to
            identify fish, an SH3 and PX domain-containing protein, and Src
            substrate
  JOURNAL   EMBO J 17 (15), 4346-4357 (1998)
   PUBMED   9687503
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132268.3 and AC132288.5.
            
            On Jan 5, 2023 this sequence version replaced NM_008018.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ007012.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-379               AC132268.3         8049-8427
            380-460             AC132268.3         47921-48001
            461-536             AC132268.3         74600-74675
            537-613             AC132268.3         99031-99107
            614-705             AC132268.3         107777-107868
            706-734             AC132268.3         129023-129051
            735-779             AC132268.3         152171-152215
            780-911             AC132268.3         158325-158456
            912-1025            AC132288.5         23548-23661
            1026-1109           AC132288.5         27162-27245
            1110-1227           AC132288.5         31836-31953
            1228-1562           AC132288.5         36748-37082
            1563-1612           AC132288.5         38209-38258
            1613-1732           AC132288.5         40309-40428
            1733-10495          AC132288.5         41330-50092
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 39.45 cM"
     gene            1..10495
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /note="SH3 and PX domains 2A"
                     /db_xref="GeneID:14218"
                     /db_xref="MGI:MGI:1298393"
     exon            1..379
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..3682
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /note="isoform 1 is encoded by transcript variant 1; SH3
                     multiple domains 1; SH3 and PX domain-containing protein
                     2A; tyrosine kinase substrate with five SH3 domains; SH3
                     multiple domains protein 1; five SH3 domain-containing
                     protein"
                     /codon_start=1
                     /product="SH3 and PX domain-containing protein 2A isoform
                     1"
                     /protein_id="NP_032044.2"
                     /db_xref="CCDS:CCDS29891.1"
                     /db_xref="GeneID:14218"
                     /db_xref="MGI:MGI:1298393"
                     /translation="MLAYCVQDATVVDVEKRRSPSKHYVYIINVTWSDSTSQTIYRRY
                     SKFFDLQMQLLDKFPIEGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCR
                     ALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKRKSVWLSSWAESPKKDVTGAD
                     TNAEPMILEQYVVVSNYKKQENSELSLQAGEVVDVIEKNESGWWFVSTSEEQGWVPAT
                     YLEAQNGTRDDSDINTSKTGEVSKRRKAHLRRLDRRWTLGGMVNRQHSREEKYVTVQP
                     YTSQSKDEIGFEKGVTVEVIRKNLEGWWYIRYLGKEGWAPASYLKKAKDDLPTRKKNL
                     AGPVEIIGNIMEISNLLNKKASGDKEAPAEGEGSEAPITKKEISLPILCNASNGSALA
                     IPERTTSKLAQGSPAVARIAPQRAQISSPNLRTRPPPRRESSLGFQLPKPPEPPSVEV
                     EYYTIAEFQSCISDGISFRGGQKAEVIDKNSGGWWYVQIGEKEGWAPASYIDKRKKPN
                     LSRRTSTLTRPKVPPPAPPSKPKEAEENPVGACESQGSPLKVKYEEPEYDVPAFGFDS
                     EPEMNEEPSGDRGSGDKHPAQPRRISPASSLQRAHFKVGESSEDVALEEETIYENEGF
                     RPYTEDTLSARGSSGDSDSPGSSSLSLAVKNSPKSDSPKSSSLLKLKAEKNAQAELGK
                     NQSNISFSSSVTISTTCSSSSSSSSLSKNNGDLKPRSASDAGIRDTPKVGTKKDPDVK
                     AGLASCARAKPSVRPKPVLNRAESQSQEKMDISSLRRQLRPTGQLRGGLKGSRSEDSE
                     LPPQMASEGSRRGSADIIPLTATTPPCVPKKEWEGQGATYVTCSAYQKVQDSEISFPE
                     GAEVHVLEKAESGWWYVRFGELEGWAPSHYLVAEENQQPDTASKEGDTGKSSQNEGKS
                     DSLEKIEKRVQALNTVNQSKRATPPIPSKPPGGFGKTSGTVAVKMRNGVRQVAVRPQS
                     VFVSPPPKDNNLSCALRRNESLTATDSLRGVRRNSSFSTARSAAAEAKGRLAERAASQ
                     GSESPLLPTQRKGIPVSPVRPKPIEKSQFIHNNLKDVYISIADYEGDEETAGFQEGVS
                     MEVLEKNPNGWWYCQILDEVKPFKGWVPSNYLEKKN"
     exon            380..460
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            461..536
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            537..613
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            614..705
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            706..734
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            735..779
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            780..911
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1025
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1109
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1227
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1562
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1612
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1732
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..10495
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10468..10473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /note="hexamer: AATAAA"
     polyA_site      10495
                     /gene="Sh3pxd2a"
                     /gene_synonym="2310014D11Rik; C230050L11; EG329070; Fish;
                     Gm5098; Sh3md1; Tks5; TSK5"
                     /note="major polyA site"
ORIGIN      
        1 agttcccgcc ggccccaccg agcggcagag agagcgggca ggcgagcgga gcgcggcggc
       61 gcgggaggga aggggcgcgc ggcaggccca cgggagcagc ctgggacgca cggagccctc
      121 gtgccctccg ggacagtcgc ctgccccgga gcctcagctg taggcgcccg gagcctcggc
      181 ggcgagtagc gcgtggggca ggggtggcgt gtaactgcag gaaccggggc aaccggagaa
      241 ggagccggaa cccggccggg atgagaaggt gacgccgccg gggggcgcca ctcgctttgt
      301 ggggacgatg ctcgcctact gcgtgcaaga tgccaccgtg gtggacgtgg agaagcggag
      361 gagcccctct aaacactatg tatacattat caacgtgacc tggtctgact ccacctccca
      421 gactatctac cggaggtaca gcaagttctt cgacctgcag atgcagcttc tggataagtt
      481 tcctattgaa ggtggccaga aggatccgaa gcaaaggatt attccctttc ttccaggcaa
      541 gatcctcttc cggagaagcc acatccgcga cgtggctgtg aagagactaa agcccatcga
      601 tgaatactgc agggcgcttg tccggctgcc gccccacatt tcacagtgtg acgaagtctt
      661 ccggttcttt gaggcacggc ctgaggatgt caaccctcca aaagaagact atggcagttc
      721 caagaggaaa tcagtgtggt tgtccagctg ggctgagtct cccaagaagg acgtgacagg
      781 tgccgacacc aacgccgagc ccatgatcct ggaacagtac gtggtggtgt ccaactataa
      841 gaaacaagag aactcggagc tgagcctcca ggccggggag gtggtagatg tcatcgagaa
      901 gaacgaaagc ggctggtggt ttgtgagcac atctgaagag caaggttggg tccccgccac
      961 ctacttggag gcccagaatg gcacacgaga cgactcggac atcaacacct ccaagactgg
     1021 ggaagtgtcc aagagacgca aggcacacct gcggcgcctg gatcgccggt ggaccctggg
     1081 cgggatggtc aacaggcagc acagccgaga agagaagtat gtcactgtgc agccctacac
     1141 cagccagagc aaagacgaga tcggcttcga gaagggtgtc accgtggagg tgattcgaaa
     1201 gaacctggaa ggctggtggt acatcaggta cctcggcaaa gagggttggg caccagcgtc
     1261 ctacctgaag aaggccaagg atgacctgcc gacccggaag aagaacctgg cgggtccggt
     1321 ggagatcata gggaacatta tggagatcag caaccttctc aacaagaagg catctgggga
     1381 taaggaggct ccggctgaag gcgagggatc cgaggccccc atcaccaaga aagagatcag
     1441 cttaccgatc ctctgcaacg cctccaatgg cagcgccttg gccattcccg agaggaccac
     1501 atccaagcta gcccagggct ccccagctgt ggccaggatc gcccctcaga gggcccagat
     1561 cagctcccca aatctgagga caagacctcc cccgcgcaga gaatccagcc tggggttcca
     1621 gctgccaaag ccgccagagc ccccttctgt tgaggtagaa tactacacca ttgctgaatt
     1681 ccagtcctgc atttctgacg ggatcagctt tcgaggcgga cagaaggcag aggtcatcga
     1741 caagaactcg ggtggttggt ggtacgtgca gatcggggag aaggagggct gggccccagc
     1801 ctcatacatt gacaagcgca agaaacccaa cctcagccgc cgaaccagca ctctgacgcg
     1861 gcccaaggtg ccgccacctg cgccccccag caagcctaag gaggccgagg agaatcctgt
     1921 gggtgcctgt gagagccagg gctccccact gaaggttaaa tacgaggaac ccgagtatga
     1981 cgtccctgcc tttggctttg actcagagcc cgagatgaat gaagagcctt caggggacag
     2041 aggttcaggt gacaagcatc ccgcccagcc ccgaaggatc tcgcctgcct cttccctgca
     2101 acgggcccat ttcaaggtgg gtgagtcttc tgaggacgtg gccctggaag aggagaccat
     2161 ctatgagaat gagggcttca ggccatacac agaagacacc ctgtctgcca gaggctcctc
     2221 tggggacagt gactcccctg ggagctcctc tttgtccctt gccgtgaaaa actcccctaa
     2281 atcagattcc cccaaatcct catcactcct aaagctcaaa gcagagaaga atgcccaggc
     2341 agaactgggg aaaaaccagt ccaacatctc cttctcctcc tctgtcacca tcagcaccac
     2401 ctgttcctcc tcctcatcct cgtcctcctt gtccaagaac aatggtgacc tgaaaccacg
     2461 ttctgcctca gatgcaggta tccgtgacac ccctaaggtt gggaccaaga aagatcctga
     2521 tgtgaaggcc gggctggcct cctgcgcccg agccaagcca tccgtgagac caaagccagt
     2581 cctgaaccga gcggagtctc aaagccagga gaagatggat attagttcct tacggcgcca
     2641 gctgaggccc acaggccagc tccggggggg cctcaagggc tctaggagtg aggactcaga
     2701 gctgcctcca cagatggctt ctgagggatc caggcgaggt tctgcggaca tcatccctct
     2761 cacggccacc actcccccgt gtgtccccaa aaaggaatgg gaagggcaag gcgccaccta
     2821 cgtgacgtgc agcgcctatc agaaggtcca ggactcggag atcagcttcc ccgaaggcgc
     2881 cgaggtgcac gtgctggaga aggcggaaag tgggtggtgg tacgtgaggt ttggggagct
     2941 ggagggctgg gctccttccc actacttggt ggccgaggag aaccagcaac ctgacacagc
     3001 tagcaaagag ggagacacag gaaagagctc gcagaacgag ggcaagtcag acagcctgga
     3061 aaagattgag aagcgtgtgc aggcgctcaa cactgtgaac cagagcaaga gggccacccc
     3121 acccatcccc tcgaagcctc ccgggggctt cggcaagacc tcgggcaccg tagcggtgaa
     3181 gatgaggaac ggggtccggc aagtggccgt caggccccaa tctgtgtttg tgtctccgcc
     3241 acccaaggac aacaacctgt cctgtgccct tcggaggaac gagtcgctaa cggccaccga
     3301 cagcctcaga ggtgtccgca ggaactcctc ctttagcacc gcacggtcag cagccgctga
     3361 ggccaagggc cgcctggccg agcgggctgc cagccagggc tcagaatcgc ccctgctgcc
     3421 tacccagcgc aaaggcatcc ctgtctcccc cgtgcgtccc aagcccatag agaagtccca
     3481 gtttatccac aacaacctca aggatgtgta catctcgatt gcagactatg agggggacga
     3541 agagacggct ggcttccagg agggggtgtc catggaggtg ctggagaaga accccaatgg
     3601 ctggtggtac tgccagatcc tggatgaggt gaagcccttc aagggctggg taccctccaa
     3661 ctaccttgag aagaagaact aatagcacag ggtccttcca gactcaacgt gctgccttgg
     3721 ctgccactgg atgagctgcg gcacgccaga cacggggatg gacaagatgg gaggggggga
     3781 gggggatgac aacattgaac cctgcagaac atgtgacccc ccaatacacc ctccagctgg
     3841 aagggagggt gtgatgggca tgcacactta ggagaaaggg aagagcagga atgccctggg
     3901 cccgggatcc ccgcattgca cgttgcatgc ggatggacca tatgtcattt agcacaggcc
     3961 atgtgggaaa agcctaatcg tgccttgctg cagatctgga aaagatgtca tgaggtgtcc
     4021 tgggtcctgc cctgctcagc cacttcagtg gctggcttct tgcctctgga agtcattctt
     4081 gcattgccct tgtgatgtgt tggagattgt attggtctct agtccaccac cgaccactga
     4141 tgggtttctg gagaactacc agctctgggc tttggcagcg tctccaaaga gctcagccct
     4201 ttctggggcc aggccacagc ctatgtttgg tagttaaaag agttcccagg tgccaacaag
     4261 gactgtctcc tcttgctgtg ctggcttggc ctggcaagga gagttgccct cctcggggca
     4321 cgtggaggac agtgtgggag ttggccgaga ggatgcgagt ctcctcctaa tctagagatt
     4381 gctcgaggat ttccgggtag gcaattcctt ctgcatgttt gggaaatggt tatcggttgg
     4441 aggtttatat atcaagcgtt tggtttgggg tttattttag aagttgtttg ggggcccgag
     4501 gggctcagcc tcacatagga gacgggtagt ggtgggtggg gtttccgttg aattcatgga
     4561 gggatgtttt atttgggttt tggtttaatg tcttttgcct ttccataaac cagattgttt
     4621 gtttaggttt ccactgtgta cacagtgccc aagcatggca ccttagcagg gagtttggaa
     4681 ctgggtaaag ccaagtcccc ggctcggtga aacaaagtgt tcaggttcct gcccaagagg
     4741 gaaagagggc agcctgctgg gtggctaatc tgcctacttc tgcccacttt ctccaacctg
     4801 ggcagaaggg accctggctg ggtcagccca gagcagacac tgtgccctta gcctgctggc
     4861 aaggtaccca agaagatgga ggactttgaa gccaggagcc aaccctatct tcctgagggg
     4921 cagcaccccc cggggaacag agaagcaaaa gagagtggag tggaaacagt cctctgtctg
     4981 ctttgggtcg tgagtcagtg gttgacattt cagaggggac cctccggaaa gaagcccgca
     5041 gtgtccttcc aggactcccc tcatgccagg agtggatgcc atacgtgcct ttgacttggg
     5101 acagatcctg tctcacagcc tgtggctgag ctgccagcat ctcctctgac caagggctgc
     5161 tggttccaac agacatggga ggaagagcgc ccctcacccc acctcccagg caggtctgcc
     5221 ttcagctccc aaccctaacc tcctggatcc ccaagccatg tttctcccac aggtggatgc
     5281 aacccactag cctcctctgt caaaagcccg tggtggtgac attgttctat tttccttttc
     5341 tgtgttcatg agtttgttta aaattgaaat tagttaattt tcttctgctg gacagtatta
     5401 aatagagtgg ggttgttgag ttaatctgct agattgcagt actaatgata gtggtatagt
     5461 gtcttcatga tattatttgt acttatttga acaataatga taaagaagtg gttcattatt
     5521 tcttcattaa tgcactttaa ggtggaatgg aaagcaacct ggagagcgaa gtgcattact
     5581 taaagatgca gcatatacac ttctcatttt aaaacagcac gtatttatta aaagaggaaa
     5641 aaagtaattt atgactattt aaaataaaat ttaaagtaga gtggctgtca ggtgagagga
     5701 ccttcgacgt agccgtctgc cagggggagg ggccacagcc tcactcctca gatgtctgca
     5761 ttgagccagc tccattagtg cgcccgcctg gctgccaggc acagagcatc tcagttggac
     5821 tggaccacca ggatccccag agccagcctt ccccacctcc cctgtccttg ccacatccca
     5881 aagattctct cacaatcccc cgtgtcctga aatcatagat ccttaaaagt gggacaggga
     5941 gggtggggga ccttagaggt cactccatac tatccattcc tgttacttta gcccaaggac
     6001 aggaaatgac tttccccaaa ttgtcaaact aatggcaggt tcctgcactg cattagtact
     6061 gtgttcctcc cctgcccctc tagtttattg tgtggtcatt gtgtttacat tgtaacatcc
     6121 agccccaggg atggctggga gttgctcagg catctgggaa aagtggggaa cgtcgccacc
     6181 tagtgtcagg atgtagactt gggcaaggag tcatagccaa ggtccctcac caccaaaccc
     6241 atcagggcac agcacaaacc ctgccagcac aaagaatact tgaaacaaga agggtgcata
     6301 ccaggccttc ccagacatgg tcgagagatg ccaggcgatg tgtccctgaa tcctagccct
     6361 ccccaggcga gagggctttt ccttagagct aatgagctgc tttgtgatat tggacagacc
     6421 acagcagctg agggaagggc ctggggctct gacccaccta gaggaaaacc agatcgaact
     6481 aaaaggaaaa aaagtggaat ctcagaaacg tccaaaccta gttttccatt tgttatgatt
     6541 gaaaatgtga aatgacattg tattgctgta tactgcaact ttaatcagaa tgagcccttc
     6601 tgaggtcatt attgaggttt atataatgcc cgaagacgca tcatctggtg ctctgttttc
     6661 tttcagttta aagacccttt tcttgtatca caggggtgga gggaaaagtc ttcaaaccgt
     6721 atcctttgcc agtagctggc ctggctacac tgcctggctc ctctgtgctc tcacagtcgg
     6781 ggagcaggag gaagctggta gtggggtgtc catcagcagc aggttcctgt ttgctccccc
     6841 caggctccct tcacaactag ggttctgtct gcaaaggcct cactctctac cccaagcctg
     6901 gccccaaggg ttcctctttc tcactcctaa tggacgaagg cattatatag cgccctcgta
     6961 ggcactttga gatgttagag agtattttac tggcatttat acccttcgct tcccctgcaa
     7021 ggcatgttac ggcgtctatc atctaaggaa aaaagacaag ggaatttcag tcaaatgtta
     7081 ttttcatatg actagtactt gcagaataga tgtagagtta tttaagttgt agtgttctgt
     7141 tttttaggaa ggggtgggga ccaggtgaaa tcaccccgac ttttcctggg tttgactaat
     7201 atataatttt ttttcaggct actcgagccc cctgttccct ctaaagactt tgcattctca
     7261 atggaaaacg aaaacaatct gagacaggaa aagtgcaata ttaccaagag ggatttggga
     7321 cagtggagac cctgttgagt ctgtctgcag aaaatgggag gagttgtggg aggtagccac
     7381 ccgaggacag cccatcagtg ttgtccacct agaaggatgt ggcatggtgc tcttgacctc
     7441 ctgggccggt ccccagagca gaatgagaac atccactccc gttctcctgt tttgcagtca
     7501 gctgctcata gagaccggca gccccctcgc cccagcctcc tgatgtgtgt gggctatgaa
     7561 gctttggatg tgtgcttgag tctttctaaa actgtaaaac ctcagttcag cttgtgggca
     7621 ggaatcctgg ccataagcaa actcatctgg agggtgcgtg ctgtaagccc cacttaccca
     7681 gtgagagcca gttcctacag gcctgcagta ggcctggagc tgagtcctgg ccacccattc
     7741 atctcagtga cttgggcaaa aggcatgagc ctgtctctaa taacaggaac tcctgaggca
     7801 ggtgcacacg agcatccttt ctcatgcagc aaggaaaaga ggcaggaaaa gatggcttct
     7861 tgggggcgag gagcaagttc cagaactcgg ctccccaagg atagcaagca cggccttgct
     7921 ggtatgtccc gtttatatag tactgtaggc ctgctgctct gacccatctt gtgtgaattt
     7981 ctatacagat aggaagaggg accgggcccc tttttgggtc tctcccttgg aatagatatg
     8041 gctgcatagc cgtaattgga atacccatgc agctctctgg aaagacaaca gtccacatca
     8101 ctaaccaatt aagattccct cgcttttttg agggctgaga atgtgtgtgt gtgtgtgtgt
     8161 gtgtgtgtgt gtgtgtgtgt gtggtcattt ccacgagcat gggaagttca tgagtgcata
     8221 tgtgtgtgta agaagaaaga gactggtggt aattctccac ttgaatagca tggggttaga
     8281 gagaatgagt accgggcagg cagtctccat tgaactagtc cttggcattg ggcaggtccc
     8341 aagggacgcg cagcttctga caacatgtgt gtcattcaca cgattctggc tggagagtgc
     8401 aatgtgtctt taaaaatttt tttgtaatta ttttattatt tagttggaaa cttcacactg
     8461 gtattaacag agcgaacaga agcagcccag gcaggccatt gtcccgggca ccaagatgaa
     8521 gacatggaaa agatactgtt gagtatggaa gtgctgaagt gattaaatta gggcacccac
     8581 ctccatcctc tccttggcat gagacccccg gggattcatg agctactggg ttacggtttg
     8641 tttctgaagg acaggcaggc tggcagatga ggagtgagac agtagggcct gcagctgtcc
     8701 acacagcagt gcctcccgtc tccacagcga gccttccgtc tccacagcga accttagatc
     8761 gcttcctgtg agtgtggaag gctcctcatg accggctggg atttttgata agtcccgttt
     8821 ggggcaatct gatagtctca tgtccccatg tttcctaagc tcggtgctga gagtgacgtt
     8881 agcactgcta gtcacttcgt tctgtgcatc tgggtcccca gaccacaaag taggtacatt
     8941 ggtgcttgta gattgaagga aagggtcttt ccaaagcctt gatcccaacc tggctgcaag
     9001 ggattggcag gtgccaccct ctcccctctc taaatctcaa gtgaccttga cagtccgtgc
     9061 ccctccccta ttgcactcca atgccttaga gtgccccccc gcaccccccc caccccccgg
     9121 tgctgaaggt ccctgcctta ttcccaaatc atattctgga cagttggctg tgtctttgga
     9181 agtgtgagag agtcatgtta gtgcaaacag acgtgtctgg catgtgggaa agctagtggg
     9241 aggcatgttg gcaagaggag tgcgcatgtt tggagttact ggcgcttacc gtccttgcac
     9301 ggggtggaag cttccagaga tgtcttttag gtaggtgtgg gaaggctgag agagagagag
     9361 tttggaacag gaaaggagtc agttcttgga ggttgaaaac gagaggtttc cagggatttt
     9421 tgctctcacc agccaagagt ccagccagag aaaacttgtt cactgggccc catccgggct
     9481 tccagtatca ttgtcttaag gaagaggtgc tgcaagtcat acgccaatct ttgggtctgc
     9541 gggcccagtc ccatcccgga ttccccatag tttttgccca gtgcattttg tcccatgcag
     9601 agctagcctg tcaaaccata caaaatagac accactgacc ataataggcc acaccgtagg
     9661 gacccaacac ctccatacta gaccctaaag cccaggccta tctgggaggc aggtgacctc
     9721 attgggacat ttattcccaa ggctccaata gaacctacgg ttctgattgc tgagtctgcc
     9781 tcctgcctgc cagtccggcc aggcgagtgc accaccctcc tgcatgtgca tctactgagg
     9841 cacactgctt ctgagtttac ttcaaagggt acacagttgc catccttaga tttacaactt
     9901 gtctcctgca acagaggaca tgggtgtccc taccagttac tgagaggcct ggaaggtaac
     9961 ttggaaggca gaagtgagca gctactgcta ccgtgtgatg agaacgagcc ggtggatgtc
    10021 actgggactc ctaaatcaca gggatttcct agtgtccact catccccggc cagcctactg
    10081 tggtcagcag gagcccttcc cccagtttct cccctccgct ccccacctca gcagaggaac
    10141 ggagacaagg acattggatg cagtaggggg gtccgtgcaa gtgctaacat atctccagga
    10201 tcctcggtgg tgctaaccta tctccatgtt tctcataatt ctaatagtgg attctttttt
    10261 ataagcctca tcacaaacac agtgtcaccg ggggagggac ccagccccct tgacctgccc
    10321 ctttccaggt gtcctttatc actttggtgg gactctttga tctgtagaaa tgacctgtct
    10381 tggcatgctt ctagacttca acatttggat tgttttgttt cctattttgt tcgtgtttac
    10441 aggcatcttg tatttcccta aagactaaat aaagtttttg gcctttttaa ctgaa
//
