LOCUS       NM_013781               3061 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus SH2 domain containing 3C (Sh2d3c), transcript variant
            1, mRNA.
ACCESSION   NM_013781 XM_991278 XM_991308
VERSION     NM_013781.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Herbin,O., Regelmann,A.G., Ramkhelawon,B., Weinstein,E.G.,
            Moore,K.J. and Alexandropoulos,K.
  TITLE     Monocyte Adhesion and Plaque Recruitment During Atherosclerosis
            Development Is Regulated by the Adapter Protein Chat-H/SHEP1
  JOURNAL   Arterioscler Thromb Vasc Biol 36 (9), 1791-1801 (2016)
   PUBMED   27417580
  REMARK    GeneRIF: Chat-H regulates atherosclerosis development by
            controlling monocyte adhesion and recruitment to the plaques.
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   3  (bases 1 to 3061)
  AUTHORS   Browne,C.D., Hoefer,M.M., Chintalapati,S.K., Cato,M.H., Wallez,Y.,
            Ostertag,D.V., Pasquale,E.B. and Rickert,R.C.
  TITLE     SHEP1 partners with CasL to promote marginal zone B-cell maturation
  JOURNAL   Proc Natl Acad Sci U S A 107 (44), 18944-18949 (2010)
   PUBMED   20956287
  REMARK    GeneRIF: SHEP1 and CasL are constitutively associated in B cells.
REFERENCE   4  (bases 1 to 3061)
  AUTHORS   Roselli,S., Wallez,Y., Wang,L., Vervoort,V. and Pasquale,E.B.
  TITLE     The SH2 domain protein Shep1 regulates the in vivo signaling
            function of the scaffolding protein Cas
  JOURNAL   Cell Signal 22 (11), 1745-1752 (2010)
   PUBMED   20603213
  REMARK    GeneRIF: Shep1 plays a critical role in the in vivo regulation of
            Src activity and Cas downstream signaling through Crk protein.
REFERENCE   5  (bases 1 to 3061)
  AUTHORS   Wang,L., Vervoort,V., Wallez,Y., Core,N., Cremer,H. and
            Pasquale,E.B.
  TITLE     The SRC homology 2 domain protein Shep1 plays an important role in
            the penetration of olfactory sensory axons into the forebrain
  JOURNAL   J Neurosci 30 (39), 13201-13210 (2010)
   PUBMED   20881139
  REMARK    GeneRIF: Shep1 plays an important role in the early ingrowth of
            peripheral olfactory sensory axons into the forebrain.
REFERENCE   6  (bases 1 to 3061)
  AUTHORS   Regelmann,A.G., Danzl,N.M., Wanjalla,C. and Alexandropoulos,K.
  TITLE     The hematopoietic isoform of Cas-Hef1-associated signal transducer
            regulates chemokine-induced inside-out signaling and T cell
            trafficking
  JOURNAL   Immunity 25 (6), 907-918 (2006)
   PUBMED   17174122
REFERENCE   7  (bases 1 to 3061)
  AUTHORS   Dail,M., Kalo,M.S., Seddon,J.A., Cote,J.F., Vuori,K. and
            Pasquale,E.B.
  TITLE     SHEP1 function in cell migration is impaired by a single amino acid
            mutation that disrupts association with the scaffolding protein cas
            but not with Ras GTPases
  JOURNAL   J Biol Chem 279 (40), 41892-41902 (2004)
   PUBMED   15272013
  REMARK    GeneRIF: glutamic acid mutation of tyrosine 635 makes SHEP1 unable
            to promote Cas-Crk association, membrane ruffling, and cell
            migration toward epidermal growth factor (EGF), implying that these
            activities of SHEP1 depend upon a physical interaction with Cas
REFERENCE   8  (bases 1 to 3061)
  AUTHORS   Sakakibara,A., Hattori,S., Nakamura,S. and Katagiri,T.
  TITLE     A novel hematopoietic adaptor protein, Chat-H, positively regulates
            T cell receptor-mediated interleukin-2 production by Jurkat cells
  JOURNAL   J Biol Chem 278 (8), 6012-6017 (2003)
   PUBMED   12486027
REFERENCE   9  (bases 1 to 3061)
  AUTHORS   Sakakibara,A. and Hattori,S.
  TITLE     Chat, a Cas/HEF1-associated adaptor protein that integrates
            multiple signaling pathways
  JOURNAL   J Biol Chem 275 (9), 6404-6410 (2000)
   PUBMED   10692442
REFERENCE   10 (bases 1 to 3061)
  AUTHORS   Dodelet,V.C., Pazzagli,C., Zisch,A.H., Hauser,C.A. and
            Pasquale,E.B.
  TITLE     A novel signaling intermediate, SHEP1, directly couples Eph
            receptors to R-Ras and Rap1A
  JOURNAL   J Biol Chem 274 (45), 31941-31946 (1999)
   PUBMED   10542222
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772271.11.
            
            On Oct 3, 2022 this sequence version replaced NM_013781.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB043953.1, AK155165.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AL772271.11        135949-136057
            110-572             AL772271.11        139799-140261
            573-612             AL772271.11        152381-152420
            613-741             AL772271.11        159613-159741
            742-1196            AL772271.11        160697-161151
            1197-1321           AL772271.11        161884-162008
            1322-1854           AL772271.11        164015-164547
            1855-1970           AL772271.11        165814-165929
            1971-2142           AL772271.11        166376-166547
            2143-2254           AL772271.11        167476-167587
            2255-2461           AL772271.11        167851-168057
            2462-3061           AL772271.11        169258-169857
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.09 cM"
     gene            1..3061
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /note="SH2 domain containing 3C"
                     /db_xref="GeneID:27387"
                     /db_xref="MGI:MGI:1351631"
     exon            1..109
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2637
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /note="isoform 1 is encoded by transcript variant 1; SH2
                     domain-containing protein 3C; SH2 domain-containing Eph
                     receptor-binding protein 1; Cas/HEF1-associated signal
                     transducer; novel SH2-containing protein 3"
                     /codon_start=1
                     /product="SH2 domain-containing protein 3C isoform 1"
                     /protein_id="NP_038809.1"
                     /db_xref="CCDS:CCDS15928.1"
                     /db_xref="GeneID:27387"
                     /db_xref="MGI:MGI:1351631"
                     /translation="MTEMPKKTGRKFKFFKFKGLGSLSNLPRSFSLRRSSASASIRSC
                     PEPDTFEATQDDMVTLPKSPPAYARSSDMYSHMGTMPRPNIKKAQKQQAVQKAQEVSR
                     ESHLVSRRLPEPPDLEAAKEAGEGTEALLEDTAPSAVEVDPMRELEDLTVDTEKEQVP
                     GDVSPERTAAELEAAGDYVKFSKEKYILDSSPEKLHKELEEELKLSSTDLRSHAWYHG
                     RIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWHNQALHFKINKVVVKAGESYTHI
                     RYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGLSQGSS
                     KTASPASPSGSKGSHMKRRSITMTDGLTTDKVTRSDGCPNSTSLPHPRDSIRNCALSM
                     DQIPDLHSPLSPISESPSSPAYSTVTRVHAPSATPSTSAQPASPVARRSSEPQLCPGN
                     TPKPPGESDRAPHASPSHTLCKASPSPSLSSYSDPDSGHYCQLQPPVRGSREQAAGET
                     PRKARGSGERQKELLENGVSDGEWGKTFTVPVVEATSSFNLATFQSQLIPKENRPLEV
                     ALLRKVKELLSEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGR
                     QLRLDLLERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAV
                     MGALEMAQISRLEQTWMTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSNTTFPHV
                     LPFITLLECDSAPAEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAEVKLQGF
                     QARPELLEVFSTEFQMRLLWGSQGANSSQAWRYEKFDKVLTALSHKLEPAIRSSEL"
     exon            110..572
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            573..612
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            613..741
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..1196
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1321
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1854
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1970
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2142
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2254
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2461
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..3061
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3041..3046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /note="hexamer: AATAAA"
     polyA_site      3061
                     /gene="Sh2d3c"
                     /gene_synonym="Chat; Nsp3; Shep1"
                     /note="major polyA site"
ORIGIN      
        1 atcagcccct ggtgtcttga gcctggagca gccacatgct agcaaaccag agactctgca
       61 cagcttgcaa agatgacaga gatgcccaag aaaacaggca gaaagttcaa gttcttcaag
      121 ttcaagggcc ttgggagcct ttccaatctc ccgcggtcct tttctctgag acggtcttca
      181 gcctctgcta gtatccggtc ttgtccggag cctgacacct ttgaagccac acaggatgac
      241 atggtgacac tcccaaagag tcctcctgcc tatgcacgtt ctagtgacat gtacagccac
      301 atgggtacca tgccccgccc taacatcaag aaggctcaga aacaacaggc tgtccagaag
      361 gcccaggagg tcagccgaga gtcgcacctg gtttctcgaa ggctgccaga gccaccagac
      421 ttagaggcag ccaaggaggc aggggaggga actgaagccc tactggagga cacggcacct
      481 tcagcagtag aagtggaccc tatgagggaa ctggaggatc tcacagtaga cacagagaag
      541 gagcaagtcc ctggggatgt gagcccagaa aggactgctg cagagctgga ggccgctggg
      601 gactatgtga agttctccaa ggagaagtac attcttgact cctcgccaga gaaactgcac
      661 aaggagctgg aagaggaact gaagctcagc agcacggacc tccgtagcca tgcctggtac
      721 cacggacgca tccctcgtga ggtctctgag actctggtgc agcggaacgg cgacttcctc
      781 atccgagact cactcaccag cctaggggac tatgtgctca cgtgccgctg gcacaatcag
      841 gccttgcact tcaagatcaa caaggtggtg gtgaaggccg gagagagcta cacccacatc
      901 cggtacctct tcgagcagga gagcttcgac cacgtgcctg ccctcgtgcg ctaccacgtg
      961 ggcagccgta aggccgtgtc cgaacagagc ggggctatca tctactgccc tgtcaaccgc
     1021 accttcccac tgcgctacct cgaggccagc tatggcctga gccagggcag cagcaagact
     1081 gccagcccag ccagcccctc aggctccaag ggcagccaca tgaagaggcg aagcattacc
     1141 atgactgatg gactcaccac tgacaaggtc acccgcagcg atggctgccc caacagcacc
     1201 tcattacccc atcctcgaga ctccatccga aactgtgccc tcagcatgga ccagatccca
     1261 gatctccact cacccctgtc tcctatctcc gagagcccca gctctcctgc ctatagcact
     1321 gtaacccgag tccatgctcc ttctgccacc ccctctactt cagcacagcc tgcctcccca
     1381 gttgcccgcc gctccagcga acctcagcta tgtcctggaa atactccaaa gcctcctggg
     1441 gagtcagaca gggcccctca cgcaagccca tcccacaccc tctgcaaggc ctcgccatca
     1501 ccatcactca gcagctacag tgacccggac tctggtcact actgtcagct ccagcctccg
     1561 gtccgtggca gccgagagca ggccgcagga gagactcccc ggaaggctag gggctccgga
     1621 gagaggcaaa aggaactgtt agaaaatgga gtctctgacg gggagtgggg caagaccttc
     1681 acagtccctg tagtagaagc cacctcctct ttcaacctgg ctaccttcca gtcccagctg
     1741 atccccaagg agaaccggcc tctggaggtg gcccttctgc gcaaggtcaa ggagctgctg
     1801 tctgaggtgg atgcccggac actggcccgg catgtcacca aggttgactg cctggttgct
     1861 aggatactgg gcgttaccaa ggagatgcag accctaatgg gagtccgctg gggcatggag
     1921 ctgctcaccc tcccccatgg ccggcagcta cgactagacc tgctggaaag gttccacacc
     1981 atgtccatca tgctggcagt ggatatcctg ggctgcacag gctcggcgga ggagcgggca
     2041 gctctgctgc acaagaccat acagctggcg gccgagctgc gggggacgat gggcaacatg
     2101 ttcagcttcg ccgctgtcat gggcgccctg gagatggccc agatttcaag gctggagcag
     2161 acatggatga ccctgcgaca gcgacacacg gagggtgcca ttctgtacga gaagaagctc
     2221 aagcctttcc tcaagagcct gaacgagggc aaagaaggcc caccgctgag caacaccacg
     2281 tttccacacg tacttccttt cattactctg ctggaatgtg actctgcccc cgccgagggt
     2341 cctgaacctt ggggcagcac agaacacggt gtggaggttg tgctagccca cctggaggcc
     2401 gcccgcactg tggcccacca cgggggcctc taccacacca acgctgaggt caagctgcaa
     2461 gggttccaag cccgaccgga gctcctggaa gtattcagca cagagttcca gatgcggctc
     2521 ctctggggca gccagggtgc caacagcagc caggcctggc gctatgagaa gtttgacaaa
     2581 gtcctcactg ccttgtccca caagctggaa cctgccatcc gctctagcga gctgtgaccc
     2641 cggagatctc gcctctctgc agctgcgggc agcatcggcg gcagaggggc tcaggacatt
     2701 ccctccagag caccacaggg acactgtgat cagcccaaga atgttctggg ttcaaatcca
     2761 ggatctccct tgcacttcca gggtcctgaa tggactctga gctccattct tccatcacac
     2821 acgcgccaag tctcccttcc aaatggacag gaacctctgc tcctcccacc ggcttgcgct
     2881 ggcccccctt ttgctgaggt tccctgtttt tgagccatgg gaggctcccc acccctcttc
     2941 cttcctgcag gcatctatgc ctagaggtgc cacagcctcc atcttactgt aaataagtct
     3001 ggtgtaaatg tatgtacaga agccatgctc tctttcatat aataaacttt aatgactctt
     3061 t
//
