LOCUS       NM_001411077            4611 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus SH2 domain containing 2A (Sh2d2a), transcript variant
            3, mRNA.
ACCESSION   NM_001411077 XM_006501555
VERSION     NM_001411077.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Yi,F.S., Zhang,X., Zhai,K., Huang,Z.Y., Wu,X.Z., Wu,M.T., Shi,X.Y.,
            Pei,X.B., Dong,S.F., Wang,W., Yang,Y., Du,J., Luo,Z.T. and Shi,H.Z.
  TITLE     TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant
            Pleural Effusion by Regulating TH1/TH17 Cell Response
  JOURNAL   J Immunol 205 (10), 2926-2935 (2020)
   PUBMED   33046503
  REMARK    GeneRIF: TSAd Plays a Major Role in Myo9b-Mediated Suppression of
            Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Smith,R.O., Ninchoji,T., Gordon,E., Andre,H., Dejana,E.,
            Vestweber,D., Kvanta,A. and Claesson-Welsh,L.
  TITLE     Vascular permeability in retinopathy is regulated by VEGFR2 Y949
            signaling to VE-cadherin
  JOURNAL   Elife 9, e54056 (2020)
   PUBMED   32312382
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4611)
  AUTHORS   Wedel,J., Stack,M.P., Seto,T., Sheehan,M.M., Flynn,E.A.,
            Stillman,I.E., Kong,S.W., Liu,K. and Briscoe,D.M.
  TITLE     T Cell-Specific Adaptor Protein Regulates Mitochondrial Function
            and CD4+ T Regulatory Cell Activity In Vivo following
            Transplantation
  JOURNAL   J Immunol 203 (8), 2328-2338 (2019)
   PUBMED   31541025
  REMARK    GeneRIF: The TSAd serves as a critical cell-intrinsic molecule in
            CD4(+)Foxp3(+) Tregs to regulate the translocation of Lck to
            mitochondria, cellular activation responses, and the development of
            immunoregulation following solid organ transplantation.
REFERENCE   4  (bases 1 to 4611)
  AUTHORS   Abrahamsen,G., Sundvold-Gjerstad,V., Habtamu,M., Bogen,B. and
            Spurkland,A.
  TITLE     Polarity of CD4+ T cells towards the antigen presenting cell is
            regulated by the Lck adapter TSAd
  JOURNAL   Sci Rep 8 (1), 13319 (2018)
   PUBMED   30190583
  REMARK    GeneRIF: data suggest that TSAd modulates differentiation of
            experienced T cells possibly through polarization of CD4+ T cells
            towards the APC.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4611)
  AUTHORS   Gordon,E.J., Fukuhara,D., Westrom,S., Padhan,N., Sjostrom,E.O., van
            Meeteren,L., He,L., Orsenigo,F., Dejana,E., Bentley,K.,
            Spurkland,A. and Claesson-Welsh,L.
  TITLE     The endothelial adaptor molecule TSAd is required for VEGF-induced
            angiogenic sprouting through junctional c-Src activation
  JOURNAL   Sci Signal 9 (437), ra72 (2016)
   PUBMED   27436360
  REMARK    GeneRIF: These results revealed that vascular sprouting and
            permeability are both controlled through the VEGFR2-TSAd-c-Src
            signaling pathway in a subset of tissues, which may be useful in
            developing strategies to control tissue-specific pathological
            angiogenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4611)
  AUTHORS   Marti,F., Lapinski,P.E. and King,P.D.
  TITLE     The emerging role of the T cell-specific adaptor (TSAd) protein as
            an autoimmune disease-regulator in mouse and man
  JOURNAL   Immunol Lett 97 (2), 165-170 (2005)
   PUBMED   15752554
  REMARK    GeneRIF: TSAd appears to contribute to interleukin-2 synthesis at
            multiple different levels
            Review article
REFERENCE   7  (bases 1 to 4611)
  AUTHORS   Zucchelli,S., Holler,P., Yamagata,T., Roy,M., Benoist,C. and
            Mathis,D.
  TITLE     Defective central tolerance induction in NOD mice: genomics and
            genetics
  JOURNAL   Immunity 22 (3), 385-396 (2005)
   PUBMED   15780994
REFERENCE   8  (bases 1 to 4611)
  AUTHORS   Drappa,J., Kamen,L.A., Chan,E., Georgiev,M., Ashany,D., Marti,F.
            and King,P.D.
  TITLE     Impaired T cell death and lupus-like autoimmunity in T
            cell-specific adapter protein-deficient mice
  JOURNAL   J Exp Med 198 (5), 809-821 (2003)
   PUBMED   12953096
  REMARK    GeneRIF: role of TSAd as a critical regulator of T cell death whose
            absence promotes systemic autoimmunity
REFERENCE   9  (bases 1 to 4611)
  AUTHORS   Rajagopal,K., Sommers,C.L., Decker,D.C., Mitchell,E.O.,
            Korthauer,U., Sperling,A.I., Kozak,C.A., Love,P.E. and
            Bluestone,J.A.
  TITLE     RIBP, a novel Rlk/Txk- and itk-binding adaptor protein that
            regulates T cell activation
  JOURNAL   J Exp Med 190 (11), 1657-1668 (1999)
   PUBMED   10587356
REFERENCE   10 (bases 1 to 4611)
  AUTHORS   Choi,Y.B., Kim,C.K. and Yun,Y.
  TITLE     Lad, an adapter protein interacting with the SH2 domain of p56lck,
            is required for T cell activation
  JOURNAL   J Immunol 163 (10), 5242-5249 (1999)
   PUBMED   10553045
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161454.3.
            
            On Aug 22, 2022 this sequence version replaced XM_006501555.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189676.136617.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                AC161454.3         28680-28774         c
            96-250              AC161454.3         27996-28150         c
            251-432             AC161454.3         27248-27429         c
            433-522             AC161454.3         26635-26724         c
            523-739             AC161454.3         26308-26524         c
            740-868             AC161454.3         23936-24064         c
            869-4611            AC161454.3         19978-23720         c
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.78 cM"
     gene            1..4611
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /note="SH2 domain containing 2A"
                     /db_xref="GeneID:27371"
                     /db_xref="MGI:MGI:1351596"
     exon            1..95
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1210
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /note="isoform 3 is encoded by transcript variant 3; T
                     cell-specific adapter protein; SH2 domain-containing
                     protein 2A; p56Lck-associated adapter protein;
                     Rlk/Itk-binding protein; Lck-associated adapter protein;
                     SH2 domain protein 2A"
                     /codon_start=1
                     /product="SH2 domain-containing protein 2A isoform 3"
                     /protein_id="NP_001398006.1"
                     /db_xref="GeneID:27371"
                     /db_xref="MGI:MGI:1351596"
                     /translation="MEFCLAQPCPQGNHEATSSTFNTFQPMNLTQGRCQNLSCGSRPS
                     MQVMKEQGVQLSPRTNHTVVSASAPGTAWVLGNADRAEEVPGKGDLSLQAETRAWVQK
                     TQAHWLLLKTAPLWFHGFITRREAERLLQPQPLGCYLVRFSESAVTFVLSYSLVGEEM
                     ASLTQSGPSRSQTCCRHFLLAQLGDGRHVVLGEDSAHAQLQDLLEHYTECPLSPYGEI
                     LTQPLARQTAEPAGLSLRADSDSGSKRQDPDTQLSLLLQQGQAQASGHTEKTSQASRP
                     RPPIPAKPQLPPEVYTSPASRLHQAPPINPIYQEPDEPIAFYAMGRGSPGDAPSNIYA
                     EVEGPSGTAPIGHPILRKCWSRPISRGQVREVQGKISSRSRAERGSPS"
     exon            96..250
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     exon            251..432
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     exon            433..522
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     exon            523..739
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     exon            740..868
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     exon            869..4611
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1547..1552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /note="hexamer: ATTAAA"
     polyA_site      1575
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /note="major polyA site"
     regulatory      4589..4594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
                     /note="hexamer: AATAAA"
     polyA_site      4611
                     /gene="Sh2d2a"
                     /gene_synonym="Lad; Ribp; Tsad"
ORIGIN      
        1 agaggagaga tgagcagctg ccgagtgtgc acctcctttg agagcaccca cacctgcttc
       61 catggagttc tgcttggccc aaccatgccc ccaagggaat catgaagcta cttcatcaac
      121 cttcaacacc ttccagccaa tgaacctgac ccaggggaga tgccagaact tgagttgtgg
      181 gagcaggccc agcatgcaag ttatgaagga acagggggtt cagctcagcc ccagaactaa
      241 ccacacggtg gtctctgcct cagccccagg aactgcctgg gtcctaggga atgccgatag
      301 ggcagaagag gtgcctggga aaggtgacct gtcattgcaa gctgagacca gagcatgggt
      361 ccagaagacc caggcccact ggctcttgct caagactgcc cctctttggt tccatggctt
      421 catcaccaga agggaagccg agaggctgct gcagccccag cctctaggat gctatctggt
      481 gcgcttcagc gagagtgctg tgaccttcgt gctgtcctac agcctggtgg gggaagaaat
      541 ggcaagtctg actcaaagtg gcccctccag gagccagacc tgctgccgtc acttcctcct
      601 cgcgcagcta ggggatgggc gccacgtggt gctaggtgaa gacagtgccc atgcgcagct
      661 tcaggacctg cttgaacatt acacagagtg ccccctcagc ccctacggag agatactcac
      721 ccaacccctc gctcgacaga cagctgagcc tgctggactt tccctaaggg ctgattcaga
      781 ctctggaagc aaaagacagg acccagacac ccagctcagc ctgctcctcc aacagggcca
      841 ggcccaggcc tcaggacaca cagagaaaac atcccaggca tccagaccca ggccgcccat
      901 ccccgccaag cctcagcttc ctcctgaagt ctatacaagt cctgcttcac gactgcatca
      961 ggccccaccc attaacccca tctaccagga gcctgatgaa cccatagcct tctatgccat
     1021 ggggcggggc agccctgggg acgctcctag taatatctat gctgaggtgg aagggccatc
     1081 gggaacggcg cccattggac accccatcct ccgaaagtgc tggtccaggc ctatctcaag
     1141 aggccaggta agggaggtac agggaaagat aagctcaagg agtcgagcag aaagagggag
     1201 cccctcctga atatcgggca aggtgaaggt cagcaggctg aagcgagatg gagtgttcca
     1261 cgctgccgaa aacctttgcc cagctccagg cctaaggctc ttcctggaca ttcacgtgga
     1321 ctcagctttg tcaagcccta cctgccccta cttcaggttt ccttcaacca ctctccatcc
     1381 caggctgcct ctcattggtc aggccttcct cacacacatg tgacactgac ccagctccct
     1441 ggagagcgtc actgccagtg ggctcagagg aggaggctgc cacactgtgt gctcaccctc
     1501 acagtttccc cacagagttt cagtcatgtc tgaatgtggt attactatta aaggagtcag
     1561 actctttccc ttgtacctgc gtcaggtcct acttcaggct tcctctctgc tgccaccaaa
     1621 ggtccctgct tggccctcac cagcccagct cacctgtacc ctaccagcct tcttaaccct
     1681 gggtccatcc aggctctgcc agggttccaa agccactcca acatcaattg aaccaacctc
     1741 actcgcaccc tgtgtgggcg cgcgcatgag cgcgcgcgcg cacacacaca cacacacatg
     1801 caccacgcac agtgtcctgt gttccacacc ctgattgatt aacccccagc acccagctat
     1861 ccagccagaa atgtggggtc cctaaacatc tccatcccct ttgcttcagt ctctgtctgt
     1921 caataatgtc agttgcctct acctcccaag ttctggaatt accactgtgt gctcctacag
     1981 tatgtgggat caaacttggg gcttggtacg tgctagacaa gcactctatg tctgagccac
     2041 accatgattt cactgagtgg cttaactgcc ttcctagtac caggcttgtc agctctgacc
     2101 tgttcttccc cgaggaagcc aaaatgggct ctgtgagtca gctgcctggt cctccagctc
     2161 ttccatactg aggcgaggtc ccacttcgtc agccccactt caagcaccca caccatattt
     2221 ctccaaactc accaaaggct accaagtcag tcctgcccac agcaatccct acaagaccgc
     2281 cctcctgctg cctggcaacc cttaacccca ctcaagacca caagcccttc cccttgcaaa
     2341 gccttccctg gcctgacctc ccaacacacc cttgccaggc ccctgctcag cccagactcc
     2401 cattgctctt tttttcttct atctcccctt cctgcagagc ctgattcact agaggcagcc
     2461 ctgggggatg ctcagctatc tctaaagtac tggcccagtg cttggcatga agcaactgcc
     2521 tcagggaggg agggggaagg gagaagagga gaggcagagg gatggacggg aacctaaatc
     2581 ccctctggag gccaaagggt taagaccata taccttctcc tagagactgt agctctgcca
     2641 aagaggcaga gggtatgacc cagagctgtc cttctgtctg cagaggacag atggtccaga
     2701 gctgcattct gaggactgtg tggcagagta agaccctgtc cttcatcatg agcccccctg
     2761 gccctagagg cttgcccttt ctagacagag agcactcatg gctccccatc aggccttatt
     2821 aggggtaagt ctgatctggg agggtagctt ggggcatttt gtgtcctgag accttcacag
     2881 acccctgggt cctctcttag ggaaggcagg tgggatagac tacaggtgag cagagcttca
     2941 tctgctacac ggatggcgag gaggtgtcac cccgcgtgcc agctccaatc cattggtctc
     3001 ggctgcctgg catgcagcat tggaaggata ttaaggtcag atctaagctc agcagaaagg
     3061 agcaggtgac ggatgagtgg cgtctgctgt gggtgcggga agggtgagca gcgtttcttt
     3121 ctctcctatc aattaagtcc agtcctctca ttatgcaaat caagaaacaa gccagagaag
     3181 gagaaagcct gggccaaggt caaggattga aacccctctc agcattcccc atccgtcctc
     3241 tgaatcctca cttctccaca agggacagag agatttcctc agctaagaag ctggttgctc
     3301 tccagagttc tgcactggga cctccctgca ctccaccttt ccctgtcccc ttaacaagtc
     3361 tgccttcctt gctccaaagc agcctggaag tcagaggacc aaatgtggga tctgtgcttc
     3421 ctcatagcaa cacacacacc acacacacac acacacacac acacacacac acacacacac
     3481 acacacacat gcatgcatac acacacacac atacacacac gaatacacac acatgcttgc
     3541 atacacacac acatgcacat acacacatac atgcatacac acacataaac acatacacac
     3601 acacatgcat acttgcatac acacacacac acagacactc acactgctcc cttctgaaag
     3661 tcaccaggaa ccaaggcaag gaactgtatg tactagggaa gccatcctgc ctcctgtgcc
     3721 ctggtttttc ctcctgaaga aactggactt taggaagcct tggcactctg agccttcgca
     3781 tctgggcccc gatccaatgg ctgctgttgt ctgagaaatg ttttggtgag aacagatgtt
     3841 aggatttgtt tgttggtcct tgtctggcta ataaatcatt agcaacaatc tccctcctcg
     3901 ggaatctctg tccttccagc tcctccccag cccttttctc tctcccactg tcgctcatag
     3961 gggtctagag gttcaaagac acttaggcaa agttattgat cttttggctg aaagttaaaa
     4021 accatgagga caaggacacg gactcccttt gccaagtgct tctcccttag ctggggtagg
     4081 ctgggtgggc agcccgggca caggagtggg tgcctataga gatgggcaac tcaggacttg
     4141 ggagaagggt gccttagcaa tctgtgagcc ctcagaggag gcagcttaag tccttacctc
     4201 actcaggagc ccatccccct ccctgcccca ttctatgggc ctttctccct tctctgccca
     4261 gactcacttc aggactggct tcctgcccta gagctaaact cctctagagt cctagcccgg
     4321 gagcagtctg tgctaggtcc cactttcatc tcactggagg ttttctgagg ctggccatgc
     4381 atggccctac tggctccaga gtcacccgag tctctggagg tagcctttgc cactgtttct
     4441 ctctctgtgt agctccccac acagctgtta ccccgattta ctacagctca tttaaggtaa
     4501 catgtgcttt ttgtgtcctg tctcaaaaac aaaaaaacaa aacaaacgaa aataatggac
     4561 tggccctctg aacctgtaag tcagccccaa taaaatgttg tctttgtaag a
//
