LOCUS       NM_001306126            4468 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus SH2B adaptor protein 3 (Sh2b3), transcript variant 2,
            mRNA.
ACCESSION   NM_001306126
VERSION     NM_001306126.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Dou,H., Wang,R., Tavallaie,M., Xiao,T., Olszewska,M.,
            Papapetrou,E.P., Tall,A.R. and Wang,N.
  TITLE     Hematopoietic and eosinophil-specific LNK(SH2B3) deficiency
            promotes eosinophilia and arterial thrombosis
  JOURNAL   Blood 143 (17), 1758-1772 (2024)
   PUBMED   38096361
  REMARK    GeneRIF: Hematopoietic and eosinophil-specific LNK(SH2B3)
            deficiency promotes eosinophilia and arterial thrombosis.
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Zhang,Y., Morris,R., Brown,G.J., Lorenzo,A.M.D., Meng,X.,
            Kershaw,N.J., Kiridena,P., Burgio,G., Gross,S., Cappello,J.Y.,
            Shen,Q., Wang,H., Turnbull,C., Lea-Henry,T., Stanley,M., Yu,Z.,
            Ballard,F.D., Chuah,A., Lee,J.C., Hatch,A.M., Enders,A.,
            Masters,S.L., Headley,A.P., Trnka,P., Mallon,D., Fletcher,J.T.,
            Walters,G.D., Sestan,M., Jelusic,M., Cook,M.C., Athanasopoulos,V.,
            Fulcher,D.A., Babon,J.J., Vinuesa,C.G. and Ellyard,J.I.
  TITLE     Rare SH2B3 coding variants in lupus patients impair B cell
            tolerance and predispose to autoimmunity
  JOURNAL   J Exp Med 221 (4) (2024)
   PUBMED   38417019
REFERENCE   3  (bases 1 to 4468)
  AUTHORS   Dou,H., Kotini,A., Liu,W., Fidler,T., Endo-Umeda,K., Sun,X.,
            Olszewska,M., Xiao,T., Abramowicz,S., Yalcinkaya,M., Hardaway,B.,
            Tsimikas,S., Que,X., Bick,A., Emdin,C., Natarajan,P.,
            Papapetrou,E.P., Witztum,J.L., Wang,N. and Tall,A.R.
  TITLE     Oxidized Phospholipids Promote NETosis and Arterial Thrombosis in
            LNK(SH2B3) Deficiency
  JOURNAL   Circulation 144 (24), 1940-1954 (2021)
   PUBMED   34846914
  REMARK    GeneRIF: Oxidized Phospholipids Promote NETosis and Arterial
            Thrombosis in LNK(SH2B3) Deficiency.
            Erratum:[Circulation. 2022 Jul 26;146(4):e13. doi:
            10.1161/CIR.0000000000001087.. PMID: 35877838]
REFERENCE   4  (bases 1 to 4468)
  AUTHORS   Allenspach,E.J., Shubin,N.J., Cerosaletti,K., Mikacenic,C.,
            Gorman,J.A., MacQuivey,M.A., Rosen,A.B.I., Timms,A.E.,
            Wray-Dutra,M.N., Niino,K., Liggitt,D., Wurfel,M.M., Buckner,J.H.,
            Piliponsky,A.M. and Rawlings,D.J.
  TITLE     The Autoimmune Risk R262W Variant of the Adaptor SH2B3 Improves
            Survival in Sepsis
  JOURNAL   J Immunol 207 (11), 2710-2719 (2021)
   PUBMED   34740959
  REMARK    GeneRIF: The Autoimmune Risk R262W Variant of the Adaptor SH2B3
            Improves Survival in Sepsis.
REFERENCE   5  (bases 1 to 4468)
  AUTHORS   Morales,C.E., Stieglitz,E., Kogan,S.C., Loh,M.L. and Braun,B.S.
  TITLE     Nf1 and Sh2b3 mutations cooperate in vivo in a mouse model of
            juvenile myelomonocytic leukemia
  JOURNAL   Blood Adv 5 (18), 3587-3591 (2021)
   PUBMED   34464969
  REMARK    GeneRIF: Nf1 and Sh2b3 mutations cooperate in vivo in a mouse model
            of juvenile myelomonocytic leukemia.
REFERENCE   6  (bases 1 to 4468)
  AUTHORS   Takaki,S., Tezuka,Y., Sauer,K., Kubo,C., Kwon,S.M., Armstead,E.,
            Nakao,K., Katsuki,M., Perlmutter,R.M. and Takatsu,K.
  TITLE     Impaired lymphopoiesis and altered B cell subpopulations in mice
            overexpressing Lnk adaptor protein
  JOURNAL   J Immunol 170 (2), 703-710 (2003)
   PUBMED   12517931
  REMARK    GeneRIF: Lnk overexpression in transgenic mice results in impaired
            expansion of lymphoid precursor cells and altered mature B cell
            subpopulations.
REFERENCE   7  (bases 1 to 4468)
  AUTHORS   Velazquez,L., Cheng,A.M., Fleming,H.E., Furlonger,C., Vesely,S.,
            Bernstein,A., Paige,C.J. and Pawson,T.
  TITLE     Cytokine signaling and hematopoietic homeostasis are disrupted in
            Lnk-deficient mice
  JOURNAL   J Exp Med 195 (12), 1599-1611 (2002)
   PUBMED   12070287
REFERENCE   8  (bases 1 to 4468)
  AUTHORS   Takaki,S., Morita,H., Tezuka,Y. and Takatsu,K.
  TITLE     Enhanced hematopoiesis by hematopoietic progenitor cells lacking
            intracellular adaptor protein, Lnk
  JOURNAL   J Exp Med 195 (2), 151-160 (2002)
   PUBMED   11805142
  REMARK    GeneRIF: Lnk plays critical roles in the expansion and function of
            early hematopoietic progenitors
            Erratum:[J Exp Med 2002 May 6;195(9):1229]
REFERENCE   9  (bases 1 to 4468)
  AUTHORS   Takaki,S., Sauer,K., Iritani,B.M., Chien,S., Ebihara,Y., Tsuji,K.,
            Takatsu,K. and Perlmutter,R.M.
  TITLE     Control of B cell production by the adaptor protein lnk. Definition
            Of a conserved family of signal-modulating proteins
  JOURNAL   Immunity 13 (5), 599-609 (2000)
   PUBMED   11114373
REFERENCE   10 (bases 1 to 4468)
  AUTHORS   Takaki,S., Watts,J.D., Forbush,K.A., Nguyen,N.T., Hayashi,J.,
            Alberola-Ila,J., Aebersold,R. and Perlmutter,R.M.
  TITLE     Characterization of Lnk. An adaptor protein expressed in
            lymphocytes
  JOURNAL   J Biol Chem 272 (23), 14562-14570 (1997)
   PUBMED   9169414
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113302.13.
            
            On Mar 11, 2022 this sequence version replaced NM_001306126.1.
            
            Summary: This gene encodes a member of the SH2B family of adapter
            proteins that play an important role in T cell receptor signaling.
            This gene is preferentially expressed in hematopoietic stem cells,
            hematopoietic progenitors, pre and immature B cells, as well as
            megakaryocytes and mastocytes. In hematopoietic stem cells, the
            encoded protein is a key regulator of self-renewal, proliferation
            and apoptosis. Mice lacking the encoded protein exhibit pre and
            immature B cell expansion in spleen and the bone marrow.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein (isoform
            1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK171128.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-416               AC113302.13        112154-112569
            417-504             AC113302.13        115830-115917
            505-1179            AC113302.13        119344-120018
            1180-1281           AC113302.13        129212-129313
            1282-1373           AC113302.13        129390-129481
            1374-1468           AC113302.13        129566-129660
            1469-1683           AC113302.13        129741-129955
            1684-1855           AC113302.13        130033-130204
            1856-4468           AC113302.13        130327-132939
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 61.99 cM"
     gene            1..4468
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /note="SH2B adaptor protein 3"
                     /db_xref="GeneID:16923"
                     /db_xref="MGI:MGI:893598"
     exon            1..416
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            417..504
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            505..1179
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     CDS             532..2178
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /note="isoform 1 is encoded by transcript variant 2; SH2B
                     adapter protein 3; signal transduction protein Lnk;
                     lymphocyte-specific adapter protein Lnk; linker of T-cell
                     receptor pathways"
                     /codon_start=1
                     /product="SH2B adapter protein 3 isoform 1"
                     /protein_id="NP_001293055.1"
                     /db_xref="CCDS:CCDS19641.1"
                     /db_xref="GeneID:16923"
                     /db_xref="MGI:MGI:893598"
                     /translation="MNEPTVQPSRTSSAPASPASPRGWSDFCEQHAAAAARELARQYW
                     LFARAHPQPPRADLVSLQFAELFQRHFCREVRESLAGPPGHDYRATAPPRPALPKARS
                     SEDLGPRPACALQHLRRGLRQLFRRRSAGELPGATSDTNDIDTTAASRPGPARKLLPW
                     GLREPPTEALKEVVLRYSLADEAAMDSGARWQRGRLVLRSPGPGHSHFLQLFDPPKSS
                     KPKLQEACSSIREVRPCTRLEMPDNLYTFVLKVQDQTDIIFEVGDEQQLNSWLAELRA
                     STGLGLEHPDTELPLSLAAEPGPARSPRGSTDSLDQGASPGVLLDPACQKTDHFLSCY
                     PWFHGPISRVRAAQLVQLQGPDAHGVFLVRQSESRRGEYVLTFNLQGRAKHLRLVLTE
                     RGQCRVQHLHFPSVVDMLRHFQRSPIPLECGAACDVRLSGYVVVLSQAPGSSNTVLFP
                     FSLPHWDSELGHPHLSSVGCPPSHGAEALPGQVTPPEQIFHLVPSPEELANSLRQLEL
                     ESVSSARDSDYDMDSSSRGHLRAIDNQYTPLSQLCREADV"
     exon            1180..1281
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1373
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1468
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1683
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1855
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..4468
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2661
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /note="major polyA site"
     regulatory      4444..4449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /note="hexamer: ATTAAA"
     polyA_site      4468
                     /gene="Sh2b3"
                     /gene_synonym="Lnk"
                     /note="major polyA site"
ORIGIN      
        1 actagtgccc cggtgatcaa ccaaggatga tatgcggagt cctaaaggtg aaatcagcca
       61 gagatggggg aagggggtgg gacaggccat gacaggccag ggcccgctgg gggaagttga
      121 ggttataaga gttttatgct cttaggacac tttctgaacc ttgttcccca ggcctcctct
      181 gggaggaaac gccatctgag ccaggagggg tgaatacatt ctggaacttg tgcagtccca
      241 atgggtggtc agctgggaag gggctggccc gcagcagcgg cagcagctgt ggtttgggga
      301 tctcaagggc tgggcagtga caagagagga aaccgagggt cctagaggca gaacttgtgt
      361 tctttctcct ctgctctcca agatgagggt cgcgacctca gtttccccgc tgttcaggat
      421 gttcctagag tcccatgttg gagttggagt ggctggcatg tgacctgaaa ctggagtgcc
      481 agactgcctt gccctgttgc caagcccggc cgccccgcct cgctctccac catgaacgag
      541 cccaccgtgc agccgtcccg cacatcctcc gcacccgcct cgccggcatc cccacgcggc
      601 tggagcgact tctgcgagca gcacgcagca gcggcggccc gggagctggc ccgccagtac
      661 tggttgtttg cgcgcgcgca cccacagccg ccgcgcgcgg acctggtgtc gctgcagttc
      721 gcggagctct tccagcgcca cttctgccgg gaggtgcgcg agagcctcgc aggaccgccg
      781 ggtcacgact accgcgccac tgctccgccc cgccccgcgc tgcccaaggc acgcagctcc
      841 gaggacctgg gcccgcggcc cgcctgtgcc ctgcagcacc tgcgccgcgg cctgcgccag
      901 ctcttccgcc gccgctcggc aggggagctg cccggggcta ccagtgacac caatgacatc
      961 gacaccaccg cagccagcag gccgggcccg gcccgcaagt tgctaccctg gggcctgcga
     1021 gagccgccca ctgaggcgct caaggaggtc gtattgcgct atagcctggc ggacgaggca
     1081 gcaatggaca gcggcgcacg ctggcagcgg ggtcgcctgg tgcttcggtc tccaggtccg
     1141 ggccacagcc actttctgca gctcttcgat ccgcccaaga gctcaaagcc caagctccaa
     1201 gaggcctgtt ccagcatccg ggaggtccga ccatgtacac gcctggagat gcctgacaac
     1261 ctctacacct ttgtgttgaa ggtgcaggac cagacagaca tcatctttga ggtgggagat
     1321 gaacagcagc tgaactcatg gctggcagag ctcagggcaa gcacaggcct tgggctggag
     1381 cacccggaca ccgagttacc tctttcctta gcggcagagc ctggcccagc tagatcccca
     1441 aggggaagca ctgactccct ggaccaaggt gcttcacctg gggtgttgct ggacccagcc
     1501 tgccagaaaa cagatcactt cctatcctgc tacccctggt tccacggccc catctccagg
     1561 gtgagggctg cacagctggt ccagctccag ggccctgatg cccacggcgt gttcctggtg
     1621 cggcagagtg agtcccggag aggagagtat gtactcacat tcaacttaca gggcagagcc
     1681 aagcacttac gcctggtgct cacagagcgt ggacagtgcc gggtgcaaca cctgcacttc
     1741 ccctcggtgg tagatatgct ccgccacttc cagcgttctc ctatcccact ggaatgtgga
     1801 gcagcttgtg acgtccgact ctctggctat gtggtagtcc tctctcaggc accaggttcc
     1861 tccaacaccg tcctcttccc tttttccctt cctcactggg attcggagct gggtcatccc
     1921 cacctcagct ctgttggctg tccccccagc catggtgcag aggctctccc tggccaagtg
     1981 acaccacctg agcagatctt ccacctggtg ccttctcctg aggaactggc caacagtctg
     2041 cggcagctgg agctcgagtc tgtgagcagt gcccgggact cggactatga catggactcc
     2101 tcttcacggg gccaccttcg ggccattgac aaccagtaca cccctctctc acagctgtgc
     2161 agagaggcag acgtgtgaat ggaaccattt tcctcccctc cagagaacta taggctgctg
     2221 tcagccttcc ccagctcaga tgtgccccgc ttccaacaca cacaaggctg tcacaggctt
     2281 cctgtcagcc agagctagcc cccggctttc tcccactgtt tacagatgta gttcttgtgc
     2341 acaggtgcca ctagctggta ccctaggcct agtccccaac ccaaaaggag gtggggctac
     2401 aggcccagag ctggcagtgg aaactgacag agctgatgac agacttctta ccaggcaggg
     2461 tcatcgggaa gcacaaaaca ctaacagtcc ctggattctc acatggtgtc cttccctgta
     2521 gcttcagatc tgtggcaagt cctcatgggt tccatgccac aggcgatgga tgcttcatga
     2581 tcctgctcct actcttagct ttacgacaga agctctgcca gagggacaag cttaaagatc
     2641 aaaattgatt taaacatttt acctcagagt actttttttt gttttgtttt aaaggattca
     2701 ggtttccatt taatattaaa gcttattcac tgcactgccc tactaggacc cccctgtcac
     2761 tctgaagtcg ggggcagcga agcacccctg gttcgcttac cctgagccaa gctagcatgc
     2821 tagtgtcctt gtctgctccc tcaggacagt tgcctgctgt ggggtagcca ctgtcctccc
     2881 taatgtagag aatgaagtgt gtcacccttt cagggaaaac caggcagctt gcagagagga
     2941 gggtggcaag ggacattcca ctccagtgcc ttatgcatta acaaaaagga ctctgggtta
     3001 cagcagcttt acagaggctg ggaggtacag gtgggcgaag ggcttgaggt catctttata
     3061 ccctctaccc ctgaggttgg ctcctctgtg agccaaaaaa ggagctggca ctgtgttgtg
     3121 tagaagctct gtcccaggtg gcagcagggt ccccatccat acaatctctc catccagaca
     3181 gtgccaatac tgtgattgtt cctaagggac aggatcacag ggagtgggta gtgcttgcag
     3241 cagcagtggc tttagagaac cacaacttaa ctcctagaca tgttccaggg caaacattcc
     3301 gctgccctgc tgaagccagc agtactgacc tcctcctctg gttactctgt actctgcagg
     3361 ggtgtgggct ttggggaggg aagcagctgc acctcctctt gaacactccc cattgtgttc
     3421 acggaccgag gtgggtccaa cacaggtgca gattcgtgtt ccatctttaa ggtaacttgc
     3481 tttccttcac tgcatagaag gtatgaacca aagatataag caatatccaa ccagtggtcc
     3541 agattcgcct ctaatctaca taggatgggt gcacagctgt atcccttcac ctggcagaag
     3601 ctaaactaac ttcaaacttg gggcacttgt ttcccctggg ccttcagaag agagacttgc
     3661 ccgacgctgt aaaccagcac ctccaccccc actgtggaca gcttcctttc aagaagtgtc
     3721 taagataaca gaaaacataa ggcaaaaagg tattctcgtg actgtgggac agagtgacaa
     3781 cactaagcca acacctttcc tttctgctag agtaggtcca gttggctgaa ggggacgggc
     3841 aggtttgcga agctccaggc cagatgccct cccagtgtgt tggcaccagg caccgttccc
     3901 accccagcag tctttactgg ccaccactgg ctcgacagtc cccgtaccct tgcttccttc
     3961 tcctgagtac tcaactacaa actaaaagtc accagcacca aagagttggg tcaactaacc
     4021 tgccttgact ttaccagcaa tccatatggc tttatctgta taaatcttct gcctctgatc
     4081 atttctggat ggtgtaggag aaaggaatag ggatcattac aatctcgggc cacacaacac
     4141 tatctttacc taagtttctt aaagtaaggc ttcacccctt ccctgagggc tcagtctctt
     4201 gtttaatatt accttggtgc aaacaccaag tgataaactg tcacccactg cctcctagtg
     4261 aagacacatc taatggccag tgtgtggccg gaataagcag cattgtcacc acagaaacag
     4321 ggaatctcat cttctatcat tacagcagaa ggtatttggc aaatcttcgt tttatgtact
     4381 gtatgagtaa cttattcttg aataatgcaa attttgctac aatgtacaaa ttgctgtatg
     4441 tcaattaaaa aggtttccag tattacaa
//
