LOCUS       NM_053197               2974 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sideroflexin 3 (Sfxn3), transcript variant 1, mRNA.
ACCESSION   NM_053197
VERSION     NM_053197.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Ledahawsky,L.M., Terzenidou,M.E., Edwards,R., Kline,R.A.,
            Graham,L.C., Eaton,S.L., van der Hoorn,D., Chaytow,H., Huang,Y.T.,
            Groen,E.J.N., Motyl,A.A.L., Lamont,D.J., Tokatlidis,K.,
            Wishart,T.M. and Gillingwater,T.H.
  TITLE     The mitochondrial protein Sideroflexin 3 (SFXN3) influences
            neurodegeneration pathways in vivo
  JOURNAL   FEBS J 289 (13), 3894-3914 (2022)
   PUBMED   35092170
  REMARK    GeneRIF: The mitochondrial protein Sideroflexin 3 (SFXN3)
            influences neurodegeneration pathways in vivo.
REFERENCE   3  (bases 1 to 2974)
  AUTHORS   Chen,B., Aredo,B., Ding,Y., Zhong,X., Zhu,Y., Zhao,C.X., Kumar,A.,
            Xing,C., Gautron,L., Lyon,S., Russell,J., Li,X., Tang,M.,
            Anderton,P., Ludwig,S., Moresco,E.M.Y., Beutler,B. and
            Ufret-Vincenty,R.L.
  TITLE     Forward genetic analysis using OCT screening identifies Sfxn3
            mutations leading to progressive outer retinal degeneration in mice
  JOURNAL   Proc Natl Acad Sci U S A 117 (23), 12931-12942 (2020)
   PUBMED   32457148
  REMARK    GeneRIF: Forward genetic analysis using OCT screening identifies
            Sfxn3 mutations leading to progressive outer retinal degeneration
            in mice.
REFERENCE   4  (bases 1 to 2974)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2974)
  AUTHORS   Amorim,I.S., Graham,L.C., Carter,R.N., Morton,N.M., Hammachi,F.,
            Kunath,T., Pennetta,G., Carpanini,S.M., Manson,J.C., Lamont,D.J.,
            Wishart,T.M. and Gillingwater,T.H.
  TITLE     Sideroflexin 3 is an alpha-synuclein-dependent mitochondrial
            protein that regulates synaptic morphology
  JOURNAL   J Cell Sci 130 (2), 325-331 (2017)
   PUBMED   28049716
  REMARK    GeneRIF: sideroflexin 3 (SFXN3) was found to be a mitochondrial
            protein localized to the inner mitochondrial membrane.
REFERENCE   6  (bases 1 to 2974)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   7  (bases 1 to 2974)
  AUTHORS   Calvo,S.E., Pagliarini,D.J. and Mootha,V.K.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of an upstream ORF in this gene, and
            shows that it functions to reduce protein levels by ~80%.
REFERENCE   8  (bases 1 to 2974)
  AUTHORS   Yoshikumi,Y., Mashima,H., Ueda,N., Ohno,H., Suzuki,J., Tanaka,S.,
            Hayashi,M., Sekine,N., Ohnishi,H., Yasuda,H., Iiri,T., Omata,M.,
            Fujita,T. and Kojima,I.
  TITLE     Roles of CTPL/Sfxn3 and Sfxn family members in pancreatic islet
  JOURNAL   J Cell Biochem 95 (6), 1157-1168 (2005)
   PUBMED   15864810
  REMARK    GeneRIF: citrate transporter protein-like protein/Sfxn3 and some
            other family members might be important in the differentiation of
            pancreatic beta-cells
REFERENCE   9  (bases 1 to 2974)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2974)
  AUTHORS   Fleming,M.D., Campagna,D.R., Haslett,J.N., Trenor,C.C. 3rd and
            Andrews,N.C.
  TITLE     A mutation in a mitochondrial transmembrane protein is responsible
            for the pleiotropic hematological and skeletal phenotype of
            flexed-tail (f/f) mice
  JOURNAL   Genes Dev 15 (6), 652-657 (2001)
   PUBMED   11274051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY723942.1, AK034514.1, CK343762.1 and AI481047.1.
            
            On May 6, 2010 this sequence version replaced NM_053197.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012208.1, SRR1660819.79143.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            regulatory uORF        :: PMID: 19372376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-283               BY723942.1         2-284
            284-2251            AK034514.1         1-1968
            2252-2666           CK343762.1         1-415               c
            2667-2974           AI481047.1         1-308               c
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 38.26 cM"
     gene            1..2974
                     /gene="Sfxn3"
                     /note="sideroflexin 3"
                     /db_xref="GeneID:94280"
                     /db_xref="MGI:MGI:2137679"
     exon            1..315
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      241..294
                     /regulatory_class="uORF"
                     /gene="Sfxn3"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19372376]"
                     /note="this uORF is predicted to encode a 17 aa peptide
                     (PMID:19372376)"
     exon            316..479
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     CDS             319..1284
                     /gene="Sfxn3"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="sideroflexin-3 isoform 1"
                     /protein_id="NP_444427.1"
                     /db_xref="CCDS:CCDS29856.1"
                     /db_xref="GeneID:94280"
                     /db_xref="MGI:MGI:2137679"
                     /translation="MGDLPLNINIQEPRWDQSTFLGRARHFFTVTDPRNLLLSGEQLE
                     ASRNIVQNYRAGVATPGLTEDQLWRAKYVYDSAFHPDTGEKVVLIGRMSAQVPMNMTI
                     TGCMLTFYRKTPTVVFWQWVNQSFNAIVNYSNRSGDAPITVQQLGTAYVSATTGAVAT
                     ALGLKSLTKHLPPLVGRFVPFAAVAAANCINIPLMRQRELQVGIPVTDEAGQRLGHSV
                     TAAKQGIFQVVISRIGMAIPAMAIPPVIMNTLEKKDFLKRRPWLGAPLQVGLVGFCLV
                     FATPLCCALFPQRSSIHVTRLEPELRAQIQAQNPSIDVVYYNKGL"
     exon            480..650
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            651..749
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            750..825
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            826..911
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1039
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1089
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1139
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1187
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..2966
                     /gene="Sfxn3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2647..2652
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sfxn3"
                     /note="hexamer: ATTAAA"
     polyA_site      2676
                     /gene="Sfxn3"
                     /note="major polyA site"
     regulatory      2943..2948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sfxn3"
                     /note="hexamer: AATAAA"
     polyA_site      2966
                     /gene="Sfxn3"
                     /note="major polyA site"
ORIGIN      
        1 gggactgcaa gcgaaggagg caagtggggc ggctcctccc cttttctccc tggttgggaa
       61 ggaggccaga attgcttccc aggactgggt cgggtttggt ctcaacggcc tggtctggga
      121 gaatcactct ggacatccac tgtctcggaa ctctgccaag agggggggtg ggtcaggcga
      181 acgagctcag ggagcccccg cccttccctg ctgctcagcg tcacgcgtga cgtctcggtg
      241 atggctggga ggaaagcgga gagcggtgag gaaggcgggt ctgagagctt ctagaggctg
      301 aaaaccccgg aaagcaagat gggtgacctg cccttaaata tcaacatcca ggaacctcgg
      361 tgggaccaaa gcacatttct aggcagagcc cggcatttct tcacagtcac tgatccccga
      421 aatctgctgc tgtccgggga acagctggaa gcttcccgga acatcgtgca gaattacagg
      481 gctggtgtgg caaccccggg tctcactgag gaccagctat ggcgagccaa atacgtgtat
      541 gactcagcat tccatccgga cacgggggag aaggtggtct tgattggccg tatgtcagcc
      601 caggtgccca tgaacatgac cattactggc tgcatgctca ccttctacag gaagactccg
      661 actgtggtgt tctggcagtg ggtcaatcag tccttcaatg ctattgtgaa ttactctaat
      721 cgcagcggcg atgctcccat cactgtgcag cagttgggga cagcctatgt gagtgccacc
      781 actggggctg tggctactgc tctgggactc aagtctctca ccaagcacct gcccccgcta
      841 gtcggtcgat tcgtgccctt tgcagctgtg gccgctgcca actgcatcaa catccccctg
      901 atgaggcaga gggagctgca ggtgggcatc ccagtgactg atgaggctgg tcagaggctt
      961 ggccactcgg tgactgctgc caaacaggga atcttccagg tggtgatatc aagaatcgga
     1021 atggcgattc ccgccatggc cattcccccg gtgatcatga acactctgga gaagaaagac
     1081 ttcctgaagc gccgtccctg gctgggggcg cccctgcagg tgggactggt aggcttctgc
     1141 ttggtatttg ccacacccct gtgctgcgct ctgttccctc agagaagctc catacatgtg
     1201 accaggctgg agccggagct gagagctcag atccaagcac aaaaccccag catcgatgtg
     1261 gtttactaca acaaggggct ttgaggggag tcggcctctg tccctgtcct tacctcctta
     1321 ggctgcttct ctgatgccac cttgcaatgc taccacctgt ttatcttctg ggtactgggc
     1381 aagggggctt gatgtgggga gcagccactg agaccagcaa ccttagactg ggggaggcac
     1441 tcctataagc ctctccgctc ctctctggtt tcaaagagca agtcacaaaa cccatccctc
     1501 ctgtgtttgt gtgtgtgcac tgccacatat gcagcacatg tgtttataca tttcatcctg
     1561 gaagctaaga gcagaaatgc tgttctagga tgtcctgcta tttgtcttct cttctcagtc
     1621 tcctgcccac ctaacagcaa gcctggttat gggtgagact tccttcccta aactgttcct
     1681 ctagctaagt catttctgat agcatcttag ccttctgtgc ccaagataag ccccaggatc
     1741 ccagaacagg gactgaagaa catcgtaggc cacccagagc ttaacccttc aggtttcaaa
     1801 tacagctatt tgaaccagct ctaaaagctg gttcaggacg tgggctggta gaggaagcag
     1861 tgtggaatca gggaggcagg gggctcatgc ttgctggacc ccacacccta gaatggctcc
     1921 actcctctct tctggtggac catgggggtg tcagtagcct gcactgctct ccacactgca
     1981 ttgtgggtgc ttttagagac catcttgagc attcttcgtc tgttctgatg agtcatgtca
     2041 tgattcagaa atcccacact tccaattcct cccccaatca gaaagccaca caacacaggc
     2101 agagcaagca ttcccttcag gaactgccac cacccgtacc cacttgtagg ggctggtgtg
     2161 ggaccggtgc ttaaacacag gcctcaagcg tacagtatca agacaggtaa ttctgtgttt
     2221 cattgactct tcttagacac accccccccc cctcagctcc ttcctttcca tctaccccgt
     2281 ctctggaaga acccaaggct tcatatatgc taagcaactg ctctaccact gagcgacatc
     2341 cccagtgccc tctttctctt ttcctttctt tctctctttc tttctttctt tctttctttc
     2401 tttctttctt tctttctttc tttctttcct ttctttcttt ctctctctct ctctctctct
     2461 ctctctctct ctctctctct ctcccttcct tccttccttt ctgtctgaat gtgtctgtct
     2521 gtctttctgt ctgtctgtct ttctttcttt ttttgtttgt ttttgttttt cagttatggt
     2581 ttctctgggt agccctggct gtcctgaaac tcagagatct gcttgcctat ggcagtgagt
     2641 gctgggatta aaaggcatgt gccaccattg ccagcagccc gtctctttct taagacatgg
     2701 cactgcactc cctctgccct taatttcaaa aggaagaggg acccaggctc taatagctgg
     2761 accaaataga agatgagttc gggaatggtg tgggtgatac tgactccagg gcatagaatt
     2821 atatctccac gccaaatcca tgacctctag ctaaaaggtg aatggggtcc ccagtgacat
     2881 ccacggagac cacttaaact aacctcctct tccagtgaca acagctgtca cgtcatggtg
     2941 tcaataaact gcaatcctgt ctggttaaaa aaaa
//
