LOCUS       NM_175465               8951 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus SEC14 and spectrin domains 1 (Sestd1), mRNA.
ACCESSION   NM_175465
VERSION     NM_175465.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Yang,X.Y., Stanley,R.E., Ross,A.P., Robitaille,A.M., Gray,J.A. and
            Cheyette,B.N.R.
  TITLE     Sestd1 Encodes a Developmentally Dynamic Synapse Protein That
            Complexes With BCR Rac1-GAP to Regulate Forebrain Dendrite, Spine
            and Synapse Formation
  JOURNAL   Cereb Cortex 29 (2), 505-516 (2019)
   PUBMED   29293918
  REMARK    GeneRIF: Sestd1 is a developmentally dynamic synaptic regulator of
            Rho GTPases that contributes to dendrite and excitatory synapse
            formation in the forebrain.
            Erratum:[Cereb Cortex. 2019 Mar 1;29(3):1217. doi:
            10.1093/cercor/bhy026.. PMID: 29474518]
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Yang,X. and Cheyette,B.N.
  TITLE     SEC14 and spectrin domains 1 (Sestd1) and Dapper antagonist of
            catenin 1 (Dact1) scaffold proteins cooperatively regulate the Van
            Gogh-like 2 (Vangl2) four-pass transmembrane protein and planar
            cell polarity (PCP) pathway during embryonic development in mice
  JOURNAL   J Biol Chem 288 (28), 20111-20120 (2013)
   PUBMED   23696638
  REMARK    GeneRIF: Data indicate that Sestd1 cooperates with Dact1 in Vangl2
            regulation and in the planar cell polarity (PCP) pathway during
            mammalian embryonic development.
REFERENCE   3  (bases 1 to 8951)
  AUTHORS   Sun,Y.J., Nishikawa,K., Yuda,H., Wang,Y.L., Osaka,H., Fukazawa,N.,
            Naito,A., Kudo,Y., Wada,K. and Aoki,S.
  TITLE     Solo/Trio8, a membrane-associated short isoform of Trio, modulates
            endosome dynamics and neurite elongation
  JOURNAL   Mol Cell Biol 26 (18), 6923-6935 (2006)
   PUBMED   16943433
  REMARK    GeneRIF: These results suggest that Solo/Trio8 acts as an
            early-endosome-specific upstream activator of Rho family GTPases
            for neurite elongation of developing Purkinje neurons.[Trio8]
REFERENCE   4  (bases 1 to 8951)
  AUTHORS   Papin,J. and Subramaniam,S.
  TITLE     Bioinformatics and cellular signaling
  JOURNAL   Curr Opin Biotechnol 15 (1), 78-81 (2004)
   PUBMED   15102471
  REMARK    Review article
REFERENCE   5  (bases 1 to 8951)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   6  (bases 1 to 8951)
  AUTHORS   Sato,T. and Mishina,M.
  TITLE     Representational difference analysis, high-resolution physical
            mapping, and transcript identification of the zebrafish genomic
            region for a motor behavior
  JOURNAL   Genomics 82 (2), 218-229 (2003)
   PUBMED   12837271
REFERENCE   7  (bases 1 to 8951)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772242.7, BB647911.1, BG082832.2, CO425092.1, CF175139.1,
            CN664477.1 and AI848264.1.
            
            On Nov 15, 2011 this sequence version replaced NM_175465.5.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995045.494849.1,
                                           SRR7652917.1060973.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AL772242.7         6289-6326
            39-550              BB647911.1         23-534
            551-835             BG082832.2         294-578
            836-1212            CO425092.1         269-645
            1213-1810           CF175139.1         63-660
            1811-2131           CN664477.1         216-536
            2132-8763           AL772242.7         99738-106369
            8764-8951           AI848264.1         1-188               c
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 45.92 cM"
     gene            1..8951
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="SEC14 and spectrin domains 1"
                     /db_xref="GeneID:228071"
                     /db_xref="MGI:MGI:1916262"
     exon            1..38
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            39..108
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..2144
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="huntingtin-interacting protein-like protein"
                     /codon_start=1
                     /product="SEC14 domain and spectrin repeat-containing
                     protein 1"
                     /protein_id="NP_780674.1"
                     /db_xref="CCDS:CCDS16163.1"
                     /db_xref="GeneID:228071"
                     /db_xref="MGI:MGI:1916262"
                     /translation="MEASVILPILKKKLAFLSGGKDRRSGLILTIPLCLEQTSMDELS
                     VTLDYLLSIPSEKCKARGFTVIVDGRKSQWNVVKTVVLMLQNVVPAEVSLVCVVKPDE
                     FWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTEDFGGSLTYDHMDWLNKR
                     LVFEKFTKESTSLLDELALINNGSDKGNEQEKERSVDLNFLPSVDPETVLQTGHELLS
                     ELQQRRFNGSDGGVSWSPMDDELLAQPQVMKLLDSLREQYTRYQEVCRQRSKRTQLEE
                     IQQKVMQVVNWLEGPGSEQLRAQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELN
                     QQIAALLNAGDEEDLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQ
                     QLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQAS
                     WAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDASQ
                     AVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLC
                     QSLRCTSRSSGDTLPRLNRVWKQFTVASEERVHRLEMAIAFHSNAEKILQDCPEEPEA
                     MNDEEQFEEIEAIGKSLLDRLTIPVVYPDGTEQYFGSPSDMASTAEHIRDRMKLVSLK
                     RQQLRHPELVTTES"
     exon            109..217
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            218..308
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            309..422
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            423..536
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            537..634
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            635..690
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            691..902
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1025
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1220
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1335
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1495
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1577
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1700
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1892
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2014
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..8935
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3176..3181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="hexamer: AATAAA"
     polyA_site      3200
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="major polyA site"
     regulatory      8827..8832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="hexamer: AATAAA"
     polyA_site      8845
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="major polyA site"
     regulatory      8912..8917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
                     /note="hexamer: AATAAA"
     polyA_site      8935
                     /gene="Sestd1"
                     /gene_synonym="1500031J16Rik; A930010O20; Solo/Trio8"
ORIGIN      
        1 cgtccccaac gcccgccagc ccggcccgcg ccagccagag gcaaggacgt gaaatggagg
       61 cctcggtgat attgcccatt ctgaagaaga agctggcctt cctttcagga ggaaaggaca
      121 gacgcagtgg actcatcttg acaattccgc tgtgccttga gcagacaagt atggatgagc
      181 tgagtgtcac tttggattat ctactcagca ttccaagtga gaaatgtaag gccagaggat
      241 tcactgtgat cgtggatggc aggaagtctc agtggaatgt ggtgaagaca gttgtcttaa
      301 tgctgcagaa tgttgttcca gccgaggtat cccttgtttg tgtagtgaag ccagatgaat
      361 tctgggataa gaaagtaaca cacttttgtt tttggaaaga aaaggacaga cttggctttg
      421 aggttatttt agtgtccgcc aataagttga cgcgttacat agagccatgc cagttgacag
      481 aagactttgg tgggagtctc acctatgacc acatggactg gctgaataag aggctggtct
      541 ttgagaagtt tacaaaggag tctacatcat tgctagatga acttgctctg attaacaatg
      601 gaagtgataa ggggaatgag caggagaaag aaaggtctgt ggatttaaac tttcttccat
      661 ccgttgatcc tgagacggtt cttcagacag ggcatgagtt gttatctgaa ttacagcagc
      721 gtcgatttaa tggctccgac ggaggggtct cgtggtctcc tatggatgac gagctgctgg
      781 cacagccgca ggttatgaag ttgctggact cactccggga gcagtacacg cgctaccagg
      841 aagtctgcag gcagcgcagt aagcgcacac agctggagga gatccagcag aaggtgatgc
      901 aggttgtgaa ctggcttgaa gggcctggat ctgagcaact gagagcacag tggggcattg
      961 gtgactcgat cagggcctcc caggccctgc aacagaagca tgaggagatc gagagccaac
     1021 acagcgagtg gttcgcagta tacgtggagc ttaaccagca gattgcagca ctcttaaacg
     1081 ctggagacga ggaagatctt gtggagttga agtctctaca gcaacagctt agcgacgttt
     1141 gctatcgaca ggccagtcag ctagaattta ggcaaaacct cctgcaggca gctttggaat
     1201 ttcatggtgt ggcccaagat ttgtctcagc agttggacgg cttattaggg atgttgtgcg
     1261 tagatgtagc accagctgat ggagcatcga ttcagcaaac tttaaaactg cttgaagaga
     1321 agctgaaaag tgttgatgta ggattgcaag gcttgcgaga aaaaggtcaa ggtctcctgg
     1381 atcagatctc caaccaggct tcctgggcct atggcaagga tgtaactatt gaaaataaag
     1441 aaaacgtgga ccacatacaa ggagtgatgg aagacatgca acttaggaaa caaagatgtg
     1501 aggacatggt agatgtgcgg aggctgaaga tgcttcagat ggtgcagttg tttaagtgtg
     1561 aagaggatgc ttcacaggca gtagaatggc tgagtgaact tctggatgcc ctgctgaaga
     1621 cccatatcag gctgggtgac gatgctcagg aaacaaaggt tttactggaa aaacacagaa
     1681 aatttgtcga tgttgcccag agtacttacg actatggcag acagctgctg caggccacag
     1741 ttgtactgtg ccagtctctg cgctgcactt cccggtcctc aggggacaca cttcctcgac
     1801 tgaacagagt gtggaagcag tttacagtcg catctgaaga gagagtgcac aggttggaga
     1861 tggctattgc ctttcactcc aatgccgaaa agattttgca agactgccca gaagagcctg
     1921 aagcaatgaa tgatgaggaa cagtttgagg aaattgaagc aattggaaaa tcacttctgg
     1981 atagactcac tatccccgtg gtttaccccg acggaactga acaatatttt ggaagtccaa
     2041 gtgacatggc ttctactgca gagcacatca gggacagaat gaaactggtc agtctcaaaa
     2101 ggcagcagct gagacatccc gaattggtga ccacggagag ctaatgtccc cagctcccgc
     2161 agatctgcag gtcatacgcc cacattcttt gctgctggca ttctctgggg ttagccacag
     2221 ctcgttaaga tgcatttcac agcgggataa gcctcctttc ttcccacgac acgtgtctct
     2281 ctctaaatgc taacatttct ccaccaccaa ggcatagtca caacatgctt tgagactgta
     2341 gactcctggt atcctaagag aaggaattgt aaccaccgca ctgagtcctc gctttcacgc
     2401 cgtacctgac ttgtcagttt gtagccaaac aaccaatact aagctttgaa tggtgctaat
     2461 cagagtttag gaattctcta taaaacatgg ctgcccttcc tccccaggtg atgtaggata
     2521 tttagaccat agttagactg ttagtgaata gtggtgtcct caaaatttta ctttgtaatt
     2581 cttcaaaatt gattattttt aatgtctata tgaagaaaac ccttcagatc tttgatatat
     2641 catattcatt gaaagacatt ttcctattgt attcataatg tattaaaagt tgtttgtgct
     2701 taataaaaat cttctttaaa catcttattt aatttggtag ttacgtcctg cttctaaaca
     2761 cgagtgcctt actaacgggg cattcttaga attgtaagga gggttaatca ttgtttgtga
     2821 tggtggatgc atgtatttta ggcaagaatt tcatatgtat gcatgtgtat ataataaaaa
     2881 acaagcatgt ttattgtgaa gaataattta gaccattaag aacataataa tagaatacta
     2941 ttttaaactt ttctcttgaa aaatggtgtc cagaatatta gctcccctga agacactttg
     3001 tctgtgggga aggagggcta aagatgaagg tatacatact caatgtaatc ttttatatca
     3061 cagcaatcac tctggaacta aagcagaaat tgttaataat atttaatatc tcatggtatt
     3121 agaggcaaaa aaatcatcct gaagtatagt cacaaaaact gtatagatct ttagaaataa
     3181 aatttgtcat aaaatggtaa tgcttttatg tttgtattaa gttataaaat gtatatactt
     3241 taacattgtg ttaattttaa ttttagaata tattttaagg tgaaaataac tgggcttatc
     3301 agtacaaaat tatgcaaaga atcgtatgca ggtttatttt tctggtctgt aaagacgatc
     3361 acgtaccttc ctatgttcaa gccagtttct ccagccttct tttttgccag ccttgatgaa
     3421 tttcatcatc ctcaccctca ccctgtgtca cctttcagtc agctttgcgc ctcactttac
     3481 aggaaacggc catagtcttc tgggtcccgc attgaatgtt tttgctctct gacacgtcac
     3541 cttcctttcg ctccgtttct agtctctccc taacagtgct ccatccctca gtggtgctct
     3601 ctcctgccat gtgagggcat ggtttaggtc tgttgcacat gcacactgtg ctgctgtcct
     3661 cagctcttgc tcattgggca tcccatccct agtcctgcat ttcacgtccg atcccaattg
     3721 catagctttc agctgactcg gtcctctccc ctaaatgaaa gcatatccta cttgcctttt
     3781 cttcctgagt agttttgatt aatggtgcca acaatgtcca gggcaaacct cagctgttcc
     3841 ccatgtctta ctctgcctct gagaccatcc agaagtcttt tctatacttg ataacccttc
     3901 ttcagggctc gcctcctgtg cataggagct cctgtgattc tttcttcagt cttcatgatg
     3961 gtgctaactt ttcctgttac atcataaagt cctcttgggg gtgactcttg tcccacatgt
     4021 cttgtgccta gacctttgct catgtgtgac atgctcagtc ctacagctgt gttcctttgt
     4081 cagcgttagt aataatctct ccactactgc agtctgaggt ctatttaaac agtgtttggc
     4141 ttttcctagc gttagaactg acttgtaaat ctgtgtccat ggaatgtgtc tgatgtttta
     4201 gatccttaga tgtggaactt ttagatattg tttatattca gtatcagtct ctttttactg
     4261 agtttcagca tcatcagtat ctttatttga cttggtaatt atgccatcag ctcatggatg
     4321 cataatttca atttaatttt catatatctt aagccctctt ccctgcaaaa agtggaataa
     4381 gacaaaccat ggaaatgcca tgtcggactt gaaagttaaa tggaacgaga tacacagaat
     4441 ggacatagtc gctcttcttt ggcaaatgtg gacccctttt ccatgggttt atctttatag
     4501 atacacaaga gttatgaata aaaaccgagc aatgcccaaa ctgactttcc tcatgtggtc
     4561 acagttcact tagaggacat atgaaggagg tgacttcggt ttgtgtgaaa tctcaggcct
     4621 ttgttatatt aatactgaca ttgacctaaa aatgtcatta aaaagaaata cttccttctt
     4681 actggttcaa tgccgcagcc ccctgcatta ttttaaccct tctggaggtt gactgacaca
     4741 ttagctagct aacttttgtg tctggctttt tttttttttt tcttttttgc ccgtgcctat
     4801 ctttgttcct gtgtaaactc agcagacaat ggcaatttga tgtcttctct tagtacgtat
     4861 ctgactcggc agagctgtga tgcaaagacg ataacttcct ttgagaccag tttggctgca
     4921 ttcccagttc tcatcgttct gaccttattt tacaggggag tcattagtga tcttggtgag
     4981 agctcatcct gttagcttcc attcagtaca ggagagtggg ctggatctcg tacttcattg
     5041 ggattgaaag tggcttgtgg atgttatcta ctcggaatgt gtccgtcact gcagtattct
     5101 cagttgatag gtggtatgat gtcagtattg tgacccattc tcagtgagaa catgacaggt
     5161 tgctaagcaa atcttatttt ctcttctaaa cccctttgca tgctgtggta tgattgcaag
     5221 gttgggtgaa tgcatcccac cgcacaggat actttggtag agaccatcag ccttctttta
     5281 tatgctagaa gtacttgtca gaagagattg tgtgttctta cacatgtctc ccatcctacc
     5341 caaagcacac tgagtcataa taaaagcagg atggctgcta agaactaaag atacagtagc
     5401 tgatgcaatg ctgtagctct tgagttgtag agaaagtata ggctaaaata aacatccata
     5461 aatagtctgc tatccacagc aagacttgtg cgatggtgct ctgattatac atccaaaaga
     5521 aaagctctcc agagttaaaa aagatggtat gaagaaagcc aaacagcacc agtgttaact
     5581 ttcaatgcta gacataaaca ttgttgcttg agagcagaaa gccttttctt atatgtagcc
     5641 agtaaatgtc ttggtggaac aaaagtgaat accatttgac actccttgat ttctcagagt
     5701 cactttatgt cagtgaatat cctgagactc taaaatatga aatgcacaag tcatcaattt
     5761 caataacaaa atgtaataat ttacagaagt attgaaaact aaaggggcat agtatcattg
     5821 ctataatctt tttcagagta gaaagtagtg ttctttaatc ttgctctgtc tcaagtgaca
     5881 ttcatggagt ggaagagagg acacgctgtt tgtttatccc ctctgctcca tacaccttct
     5941 tcacccctac cccttatcct tacccctact ccttttaccc ctaccccttc atccctgcac
     6001 tcttcacctc tgcccgcatc tcaccagtgg ataagggaat gcgaattcag atcacatccc
     6061 gagggtggag ctggtgccct catcagtgaa tgaagcttta acgctggcac actccctgct
     6121 gtgggaagat gatggaacag taagatagga gacaagacac aaatagcatc tgtgttgggt
     6181 ttgacagggc atgagtcgac tccatttact ccactagtta gcaagccaaa gaaaggcatg
     6241 gttggagtgt agcaagaaag gaacaagcta tagaacaaga gcatacatct gaggatattt
     6301 gtaggatctc ccaataccga ggtagacatg acttgagaca accattagtg ctggctttgg
     6361 ttagaaaaga ggcagtctga caaactcagg cagtgatggt gctggggaaa ggtcctgtaa
     6421 tagagcttag acgtggcagc actggtctgt gttggtgatg ttcagtatga cttaatgctg
     6481 caggggtagt taaaggctaa tgagaataaa caccagtctg tacacttcag gaagaggcag
     6541 agcctgtgct aggacatgga gatcttcaag agaacaagag agccagcact gagactaaat
     6601 atttttgaat gctggtgaat aaatgtttca tagacacaaa attatttttc tgagaactcc
     6661 taatgagaca agctgcaata tataattctt ctatgtcatg atacaggaat tccaaacatg
     6721 gctaaaaata gttactgtca agtattaaga gcagagttgt gagggtttga agggagacgt
     6781 gtggaacagc gtgtctgaca gggctttgca ctctggttcc tactgaatct tgaggggaca
     6841 tcttaacaag tgtttacaag tccaatgcag aaccctcaca gtgactgcat agatctccct
     6901 gtagaggttg aagacaatgc ctgtccactg aaggctacaa gatcacatct ttcatgtctt
     6961 attttcctgg ctgttttagc ttgtgagaca gagcactaaa ccattcttag ggttgctttc
     7021 tctgcagaaa gtgcctgcat ctgagctcag gtgttagtct tcacagccag tgaagttgct
     7081 cagggaactg catcagggta ggtgtagttc tgagagttgt gagaggatcc aaagatcact
     7141 tgggcaggtt tgagtagatt tgggagctgc attgctctct tcagactcgg aagaaaaact
     7201 taattttcta aattggcaca tagaagtatt tggtcattat catgttgaaa gagaatttgg
     7261 ggtgggggtg gggagtaaat gtcgaaattt ctgtttcctg aagtcttaat ataaaaattt
     7321 tcagtctata aaatttaaga ttatttcatc acgtccttac caccattagt tacccttgct
     7381 ttgtcacttg cccgaccgtc tgtatgtcca tccatccaga ttatttttca gtggatctga
     7441 agctgcagat ctctgcatgc ttccatctaa acactttagc atatttctca ccagtaaggg
     7501 ttccatattt gctttctctt ggcataagat tacattcatg gagtgaagat gcacagaacc
     7561 tttccatgct ctgtttcata gttatgtggc gtgctataga aaaggacatc taccactcta
     7621 gaaagttctc ccctctcact catcactgtc cccaaatgac aagccctgtt cttcctttct
     7681 attgtgagtt agattgttct atttcagaac ttattttaat tcatatcaca catcaaggct
     7741 tctttactgc catggtattt gggaggttta tttgtggact tggtgtatta ttaattcatc
     7801 cttatgttat tactaagtgg tgtccccatg attggatatt gccacttgtt ttctcagttt
     7861 cctattggct aactgggctg tttgcaggac tgtcaactat gaacaaagct gcagtggtca
     7921 ttattaggag catacatatg aatatagtta ttcacgtgct cgtgagtaaa tacttagaat
     7981 tgctggctcg caaggtggtt tttaaaagga aactatcact ctttttccca gtgtggttgt
     8041 tctgtgccct gatttttata ttttgggtgt gtaaaaagtt gtaaacaatt ttttctgatt
     8101 ctcatgttga aatagtaaga gaagttgggg gtagaaatta tatcgctcac atttgaatgt
     8161 ggtagttggt atttgagggg agggatgctg tctgtcacaa aacagctccc ccaagcggtt
     8221 ccccagacat aaaattttag aattcgccct accctgtgag aggttaggct ctaagcatgc
     8281 taatttatag ctagaaggaa cagcacaagc ctgattatgt tgcatttacc acccactttc
     8341 agtaagtaaa gtaagacaca tcactgagta gcaaagaaca ctttatgatg gaagtggaga
     8401 gggcagaaag aaaacccaaa gcatacgcag gagtcagttc ctggtaccgt agagtgagaa
     8461 agacacctgt gcagggaagg gcccactgtg gtcaggaaag cagacaacag gatgggaaga
     8521 actctacctc acagtttaca tagcagatag ttatgttttg tagacatttt agcctttaaa
     8581 tgtcacagtc ttcatctttt cagcccacct ttgttagttt catacttggg tgaatttttt
     8641 ctactgtgta ttatcctcat cttctgttct attaaacaat atccacctgt gaaattacgg
     8701 tcatatgttt ccaataccca tgttcagata ccagtgtgtg atttaccttt cctatgggat
     8761 ttctaagggc tgggaaattt ggcttctact gtatatcctt ataatgtgcc cacattgtaa
     8821 atatgcaata aaaccataaa aaatacaagg gcagcagatt ttcatatgta tttctttgcc
     8881 atgttgtttt ttgacaagtt tgatttctgt aaataaagat tattcataaa atctaaaaaa
     8941 aaaaaaaaaa a
//
