LOCUS       NM_009255               2071 bp    mRNA    linear   ROD 06-DEC-2024
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade E,
            member 2 (Serpine2), mRNA.
ACCESSION   NM_009255
VERSION     NM_009255.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Krawczyk,M.C., Godoy,M., Vander,P., Zhang,A.J. and Zhang,Y.
  TITLE     Loss of Serpin E2 alters antimicrobial gene expression by microglia
            but not astrocytes
  JOURNAL   Neurosci Lett 811, 137354 (2023)
   PUBMED   37348749
  REMARK    GeneRIF: Loss of Serpin E2 alters antimicrobial gene expression by
            microglia but not astrocytes.
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Kastriti,M.E., Faure,L., Von Ahsen,D., Bouderlique,T.G.,
            Bostrom,J., Solovieva,T., Jackson,C., Bronner,M., Meijer,D.,
            Hadjab,S., Lallemend,F., Erickson,A., Kaucka,M., Dyachuk,V.,
            Perlmann,T., Lahti,L., Krivanek,J., Brunet,J.F., Fried,K. and
            Adameyko,I.
  TITLE     Schwann cell precursors represent a neural crest-like state with
            biased multipotency
  JOURNAL   EMBO J 41 (17), e108780 (2022)
   PUBMED   35815410
REFERENCE   3  (bases 1 to 2071)
  AUTHORS   Ehrman,J.M., Merchan-Sala,P., Ehrman,L.A., Chen,B., Lim,H.W.,
            Waclaw,R.R. and Campbell,K.
  TITLE     Formation of the Mouse Internal Capsule and Cerebral Peduncle: A
            Pioneering Role for Striatonigral Axons as Revealed in Isl1
            Conditional Mutants
  JOURNAL   J Neurosci 42 (16), 3344-3364 (2022)
   PUBMED   35273083
REFERENCE   4  (bases 1 to 2071)
  AUTHORS   Xue,M., Lin,H., Zhao,R., Fryer,C., March,L. and Jackson,C.J.
  TITLE     Activated Protein C Protects against Murine Contact Dermatitis by
            Suppressing Protease-Activated Receptor 2
  JOURNAL   Int J Mol Sci 23 (1), 516 (2022)
   PUBMED   35008942
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2071)
  AUTHORS   Selbonne,S., Madjene,C., Salmon,B., Boulaftali,Y., Bouton,M.C. and
            Arocas,V.
  TITLE     Protease nexin-1 deficiency increases mouse hindlimb
            neovascularisation following ischemia and accelerates femoral
            artery perfusion
  JOURNAL   Sci Rep 11 (1), 13412 (2021)
   PUBMED   34183729
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2071)
  AUTHORS   Carter,R.E., Cerosaletti,K.M., Burkin,D.J., Fournier,R.E.,
            Jones,C., Greenberg,B.D., Citron,B.A. and Festoff,B.W.
  TITLE     The gene for the serpin thrombin inhibitor (PI7), protease nexin I,
            is located on human chromosome 2q33-q35 and on syntenic regions in
            the mouse and sheep genomes
  JOURNAL   Genomics 27 (1), 196-199 (1995)
   PUBMED   7665170
REFERENCE   7  (bases 1 to 2071)
  AUTHORS   Wells,J.M. and Strickland,S.
  TITLE     Aprotinin, a Kunitz-type protease inhibitor, stimulates skeletal
            muscle differentiation
  JOURNAL   Development 120 (12), 3639-3647 (1994)
   PUBMED   7529678
REFERENCE   8  (bases 1 to 2071)
  AUTHORS   Mansuy,I.M., van der Putten,H., Schmid,P., Meins,M., Botteri,F.M.
            and Monard,D.
  TITLE     Variable and multiple expression of Protease Nexin-1 during mouse
            organogenesis and nervous system development
  JOURNAL   Development 119 (4), 1119-1134 (1993)
   PUBMED   8306878
REFERENCE   9  (bases 1 to 2071)
  AUTHORS   Vassalli,J.D., Huarte,J., Bosco,D., Sappino,A.P., Sappino,N.,
            Velardi,A., Wohlwend,A., Erno,H., Monard,D. and Belin,D.
  TITLE     Protease-nexin I as an androgen-dependent secretory product of the
            murine seminal vesicle
  JOURNAL   EMBO J 12 (5), 1871-1878 (1993)
   PUBMED   8491179
REFERENCE   10 (bases 1 to 2071)
  AUTHORS   Festoff,B.W., Rao,J.S. and Hantai,D.
  TITLE     Plasminogen activators and inhibitors in the neuromuscular system:
            III. The serpin protease nexin I is synthesized by muscle and
            localized at neuromuscular synapses
  JOURNAL   J Cell Physiol 147 (1), 76-86 (1991)
   PUBMED   2037625
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY065983.1, AK051332.1 and BM899460.1.
            
            On May 1, 2009 this sequence version replaced NM_009255.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051332.1, AK047856.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                BY065983.1         2-30
            30-2052             AK051332.1         2-2024
            2053-2071           BM899460.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 40.97 cM"
     gene            1..2071
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /note="serine (or cysteine) peptidase inhibitor, clade E,
                     member 2"
                     /db_xref="GeneID:20720"
                     /db_xref="MGI:MGI:101780"
     exon            1..155
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            156..436
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..1371
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /note="serine (or cysteine) proteinase inhibitor, clade E,
                     member 2; glia-derived nexin; GDN; protease nexin I;
                     serine protease-inhibitor 4; serpin E2; peptidase
                     inhibitor 7; serine (or cysteine) proteinase inhibitor,
                     clade E (nexin, plasminogen activator inhibitor type 1),
                     member 2; protease nexin 1"
                     /codon_start=1
                     /product="glia-derived nexin precursor"
                     /protein_id="NP_033281.1"
                     /db_xref="CCDS:CCDS15092.1"
                     /db_xref="GeneID:20720"
                     /db_xref="MGI:MGI:101780"
                     /translation="MNWHFPFFILTTVTLYSVHSQFNSLSLEELGSNTGIQVFNQIIK
                     SRPHENVVVSPHGIASILGMLQLGADGKTKKQLSTVMRYNVNGVGKVLKKINKAIVSK
                     KNKDIVTVANAVFLRNGFKMEVPFAVRNKDVFQCEVQNVNFQDPASASESINFWVKNE
                     TRGMIDNLLSPNLIDGALTRLVLVNAVYFKGLWKSRFQPESTKKRTFVAGDGKSYQVP
                     MLAQLSVFRSGSTRTPNGLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHITTKT
                     IDSWMNTMVPKRMQLVLPKFTAVAQTDLKEPLKALGITEMFEPSKANFTKITRSESLH
                     VSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFSIRHNPTGAILFLG
                     QVNKP"
     exon            437..664
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            665..862
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            863..1061
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1162
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1249
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1333
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..2055
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2037..2042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /note="hexamer: AATAAA"
     polyA_site      2055
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /note="major polyA site"
ORIGIN      
        1 gataaagccc ccgcgcggcc cggccggcta gtgcagtggt tgcacgggag tgcgggctgc
       61 acgcgtcacc gtcaccgccg cctgtccccc accgccgcgc agcgccgatc tccctcccgg
      121 tttcggccgc cacctgggga tccaagcgag gacgggctgt ccttgttgga aggaaccatg
      181 aattggcatt ttcctttctt catcttgacc acagtgactt tatactctgt gcactcccag
      241 ttcaactctc tgtcactgga ggaactaggc tccaacacag ggatccaggt cttcaatcag
      301 atcatcaagt cacggcctca tgagaacgtt gttgtctccc cacatgggat cgcgtccatc
      361 ttgggcatgc tgcagctcgg ggctgacggc aagacaaaga agcagctctc cacggtgatg
      421 cgatataatg taaacggagt tggtaaagtg ctgaagaaga tcaacaaggc tattgtctcc
      481 aagaaaaata aagacattgt gaccgtggcc aatgctgtgt ttctcaggaa tggctttaaa
      541 atggaagtgc cttttgcagt aaggaacaaa gatgtgtttc agtgtgaagt gcagaatgtg
      601 aacttccagg acccagcctc tgcctctgag tccatcaatt tttgggtcaa aaatgagacc
      661 aggggcatga ttgataatct gctttcccca aatctgatcg atggtgccct taccaggctg
      721 gtcctcgtta atgcagtgta tttcaagggt ttgtggaagt ctcggtttca accagagagc
      781 acaaagaaac ggacattcgt ggcaggtgat gggaaatcct accaagtacc catgttggct
      841 cagctctctg tgttccgctc agggtctacc aggaccccga atggcttatg gtacaacttc
      901 attgagctgc cctaccatgg tgagagcatc agcatgctga tcgccctgcc aacagagagc
      961 tccaccccac tgtctgccat catccctcac atcactacca agaccattga tagctggatg
     1021 aacaccatgg tacccaagag gatgcagctg gtcctaccca agttcacagc tgtggcacaa
     1081 acagatctga aggagccact gaaagccctt ggcattactg agatgtttga gccatcaaag
     1141 gcaaatttta caaaaataac aaggtcagag agccttcatg tctctcacat cttgcaaaaa
     1201 gcaaaaattg aagtcagtga agatggaacc aaagcttcgg cagcaacaac tgcaatccta
     1261 attgcaaggt catcacctcc ctggtttata gtagacaggc ctttcctgtt ttccatccga
     1321 cacaatccca caggtgccat cttgttcctg ggccaggtga acaagccctg aaggacagac
     1381 aaaggaaagc cacgcaaagc caagacgact tggctctgaa gagagactcc ctccccacat
     1441 ctttcatagt tctgttaaat atttttatat actgctttct tttttgaaac tggttcatag
     1501 cagcagttaa gtgacgcaag tgtttctggt cggggctgtg tcagaagaaa gggctggatg
     1561 cctgggatgc tggatgcctg ggatgctgga tgcctgggat gctggatgcc tgggatgctg
     1621 gatgcctggg atgctggatg cctgggatgc tgtagtgaag gatgagcagg ccggtttcac
     1681 gatgtctaga agatttcttt aaactactga tcagttatct aggttaacaa ccctctcgag
     1741 tatttgctgt ctgtcaagtt cagcatcttt gtttcattcc tgttgatatg tgtgactttc
     1801 caggagagga ttaatcagtg tggcaggaga ggttaaaaaa aaaaaagaca ttttatagta
     1861 gtttttatgt ttttatggaa aacaatatca tttgcctttt taattctttt tcctctcact
     1921 tccacccaaa ggcttgaggg tggcaaggga tggagctagc aaaagccgta gcctcttcgt
     1981 gtgttgtttc tgttgctgtt gctcttgttg ttttatatac tgcatgtgtt cactaaaata
     2041 aagttggaaa actaaaaaaa aaaaaaaaaa a
//
