LOCUS       NP_033281                397 aa            linear   ROD 06-DEC-2024
DEFINITION  glia-derived nexin precursor [Mus musculus].
ACCESSION   NP_033281
VERSION     NP_033281.1
DBSOURCE    REFSEQ: accession NM_009255.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 397)
  AUTHORS   Krawczyk,M.C., Godoy,M., Vander,P., Zhang,A.J. and Zhang,Y.
  TITLE     Loss of Serpin E2 alters antimicrobial gene expression by microglia
            but not astrocytes
  JOURNAL   Neurosci Lett 811, 137354 (2023)
   PUBMED   37348749
  REMARK    GeneRIF: Loss of Serpin E2 alters antimicrobial gene expression by
            microglia but not astrocytes.
REFERENCE   2  (residues 1 to 397)
  AUTHORS   Kastriti,M.E., Faure,L., Von Ahsen,D., Bouderlique,T.G.,
            Bostrom,J., Solovieva,T., Jackson,C., Bronner,M., Meijer,D.,
            Hadjab,S., Lallemend,F., Erickson,A., Kaucka,M., Dyachuk,V.,
            Perlmann,T., Lahti,L., Krivanek,J., Brunet,J.F., Fried,K. and
            Adameyko,I.
  TITLE     Schwann cell precursors represent a neural crest-like state with
            biased multipotency
  JOURNAL   EMBO J 41 (17), e108780 (2022)
   PUBMED   35815410
REFERENCE   3  (residues 1 to 397)
  AUTHORS   Ehrman,J.M., Merchan-Sala,P., Ehrman,L.A., Chen,B., Lim,H.W.,
            Waclaw,R.R. and Campbell,K.
  TITLE     Formation of the Mouse Internal Capsule and Cerebral Peduncle: A
            Pioneering Role for Striatonigral Axons as Revealed in Isl1
            Conditional Mutants
  JOURNAL   J Neurosci 42 (16), 3344-3364 (2022)
   PUBMED   35273083
REFERENCE   4  (residues 1 to 397)
  AUTHORS   Xue,M., Lin,H., Zhao,R., Fryer,C., March,L. and Jackson,C.J.
  TITLE     Activated Protein C Protects against Murine Contact Dermatitis by
            Suppressing Protease-Activated Receptor 2
  JOURNAL   Int J Mol Sci 23 (1), 516 (2022)
   PUBMED   35008942
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 397)
  AUTHORS   Selbonne,S., Madjene,C., Salmon,B., Boulaftali,Y., Bouton,M.C. and
            Arocas,V.
  TITLE     Protease nexin-1 deficiency increases mouse hindlimb
            neovascularisation following ischemia and accelerates femoral
            artery perfusion
  JOURNAL   Sci Rep 11 (1), 13412 (2021)
   PUBMED   34183729
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 397)
  AUTHORS   Carter,R.E., Cerosaletti,K.M., Burkin,D.J., Fournier,R.E.,
            Jones,C., Greenberg,B.D., Citron,B.A. and Festoff,B.W.
  TITLE     The gene for the serpin thrombin inhibitor (PI7), protease nexin I,
            is located on human chromosome 2q33-q35 and on syntenic regions in
            the mouse and sheep genomes
  JOURNAL   Genomics 27 (1), 196-199 (1995)
   PUBMED   7665170
REFERENCE   7  (residues 1 to 397)
  AUTHORS   Wells,J.M. and Strickland,S.
  TITLE     Aprotinin, a Kunitz-type protease inhibitor, stimulates skeletal
            muscle differentiation
  JOURNAL   Development 120 (12), 3639-3647 (1994)
   PUBMED   7529678
REFERENCE   8  (residues 1 to 397)
  AUTHORS   Mansuy,I.M., van der Putten,H., Schmid,P., Meins,M., Botteri,F.M.
            and Monard,D.
  TITLE     Variable and multiple expression of Protease Nexin-1 during mouse
            organogenesis and nervous system development
  JOURNAL   Development 119 (4), 1119-1134 (1993)
   PUBMED   8306878
REFERENCE   9  (residues 1 to 397)
  AUTHORS   Vassalli,J.D., Huarte,J., Bosco,D., Sappino,A.P., Sappino,N.,
            Velardi,A., Wohlwend,A., Erno,H., Monard,D. and Belin,D.
  TITLE     Protease-nexin I as an androgen-dependent secretory product of the
            murine seminal vesicle
  JOURNAL   EMBO J 12 (5), 1871-1878 (1993)
   PUBMED   8491179
REFERENCE   10 (residues 1 to 397)
  AUTHORS   Festoff,B.W., Rao,J.S. and Hantai,D.
  TITLE     Plasminogen activators and inhibitors in the neuromuscular system:
            III. The serpin protease nexin I is synthesized by muscle and
            localized at neuromuscular synapses
  JOURNAL   J Cell Physiol 147 (1), 76-86 (1991)
   PUBMED   2037625
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY065983.1, AK051332.1 and BM899460.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK051332.1, AK047856.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 40.97 cM"
     Protein         1..397
                     /product="glia-derived nexin precursor"
                     /note="serine (or cysteine) proteinase inhibitor, clade E,
                     member 2; glia-derived nexin; GDN; protease nexin I;
                     serine protease-inhibitor 4; serpin E2; peptidase
                     inhibitor 7; serine (or cysteine) proteinase inhibitor,
                     clade E (nexin, plasminogen activator inhibitor type 1),
                     member 2; protease nexin 1"
                     /calculated_mol_wt=41872
     sig_peptide     1..19
                     /note="/evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (Q07235.2)"
                     /calculated_mol_wt=2354
     mat_peptide     20..397
                     /product="Glia-derived nexin. /id=PRO_0000032505"
                     /note="propagated from UniProtKB/Swiss-Prot (Q07235.2)"
                     /calculated_mol_wt=41872
     Region          21..395
                     /region_name="serpinE2_GDN"
                     /note="serpin family E member 2, glia derived nexin (GDN);
                     cd19573"
                     /db_xref="CDD:381039"
     Site            93..94
                     /site_type="other"
                     /note="heparin binding site [chemical binding]"
                     /db_xref="CDD:381039"
     Site            159
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q07235.2)"
     Site            order(225,288,354..355,357..359,361..362,364..366)
                     /site_type="other"
                     /note="thrombin binding site [polypeptide binding]"
                     /db_xref="CDD:381039"
     Site            order(350..370,372..375)
                     /site_type="other"
                     /note="reactive center loop (RCL)"
                     /db_xref="CDD:381039"
     Site            364..365
                     /site_type="other"
                     /note="Reactive bond. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (Q07235.2)"
     CDS             1..397
                     /gene="Serpine2"
                     /gene_synonym="B230326M24Rik; PAI-1; PI-7; PI7; PN-1;
                     Spi4"
                     /coded_by="NM_009255.4:178..1371"
                     /db_xref="CCDS:CCDS15092.1"
                     /db_xref="GeneID:20720"
                     /db_xref="MGI:MGI:101780"
ORIGIN      
        1 mnwhfpffil ttvtlysvhs qfnslsleel gsntgiqvfn qiiksrphen vvvsphgias
       61 ilgmlqlgad gktkkqlstv mrynvngvgk vlkkinkaiv skknkdivtv anavflrngf
      121 kmevpfavrn kdvfqcevqn vnfqdpasas esinfwvkne trgmidnlls pnlidgaltr
      181 lvlvnavyfk glwksrfqpe stkkrtfvag dgksyqvpml aqlsvfrsgs trtpnglwyn
      241 fielpyhges ismlialpte sstplsaiip hittktidsw mntmvpkrmq lvlpkftava
      301 qtdlkeplka lgitemfeps kanftkitrs eslhvshilq kakievsedg tkasaattai
      361 liarssppwf ivdrpflfsi rhnptgailf lgqvnkp
//
