LOCUS       NM_009256               3397 bp    mRNA    linear   ROD 04-JUN-2023
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade B,
            member 9 (Serpinb9), mRNA.
ACCESSION   NM_009256
VERSION     NM_009256.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Dervovic D, Malik AA, Chen ELY, Narimatsu M, Adler N, Afiuni-Zadeh
            S, Krenbek D, Martinez S, Tsai R, Boucher J, Berman JM, Teng K,
            Ayyaz A, Lu Y, Mbamalu G, Loganathan SK, Lee J, Zhang L, Guidos C,
            Wrana J, Valipour A, Roux PP, Reimand J, Jackson HW and Schramek D.
  TITLE     In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of
            immune therapy response in lung cancer
  JOURNAL   Nat Commun 14 (1), 3150 (2023)
   PUBMED   37258521
  REMARK    GeneRIF: In vivo CRISPR screens reveal Serpinb9 and Adam2 as
            regulators of immune therapy response in lung cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Jiang L, Wang YJ, Zhao J, Uehara M, Hou Q, Kasinath V, Ichimura T,
            Banouni N, Dai L, Li X, Greiner DL, Shultz LD, Zhang X, Sun ZJ,
            Curtin I, Vangos NE, Yeoh ZC, Geffken EA, Seo HS, Liu ZX, Heffron
            GJ, Shah K, Dhe-Paganon S and Abdi R.
  TITLE     Direct Tumor Killing and Immunotherapy through Anti-SerpinB9
            Therapy
  JOURNAL   Cell 183 (5), 1219-1233 (2020)
   PUBMED   33242418
REFERENCE   3  (bases 1 to 3397)
  AUTHORS   Mangan MS, Melo-Silva CR, Luu J, Bird CH, Koskinen A, Rizzitelli A,
            Prakash M, Scarff KL, Mullbacher A, Regner M and Bird PI.
  TITLE     A pro-survival role for the intracellular granzyme B inhibitor
            Serpinb9 in natural killer cells during poxvirus infection
  JOURNAL   Immunol Cell Biol 95 (10), 884-894 (2017)
   PUBMED   28722018
REFERENCE   4  (bases 1 to 3397)
  AUTHORS   Mangan MS, Vega-Ramos J, Joeckel LT, Mitchell AJ, Rizzitelli A,
            Roediger B, Kaiserman D, Weninger WW, Villadangos JA and Bird PI.
  TITLE     Serpinb9 is a marker of antigen cross-presenting dendritic cells
  JOURNAL   Mol Immunol 82, 50-56 (2017)
   PUBMED   28024184
  REMARK    GeneRIF: These findings add support for role for Sb9
            cross-presentation, and indicate that high Sb9 expression is a
            novel marker of cross-presentation capable dendritic cells
REFERENCE   5  (bases 1 to 3397)
  AUTHORS   Naba A, Clauser KR, Mani DR, Carr SA and Hynes RO.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3397)
  AUTHORS   Bots M, Kolfschoten IG, Bres SA, Rademaker MT, de Roo GM, Kruse M,
            Franken KL, Hahne M, Froelich CJ, Melief CJ, Offringa R and Medema
            JP.
  TITLE     SPI-CI and SPI-6 cooperate in the protection from effector
            cell-mediated cytotoxicity
  JOURNAL   Blood 105 (3), 1153-1161 (2005)
   PUBMED   15454490
  REMARK    GeneRIF: SPI-CI is a novel immune escape molecule that acts in
            concert with SPI-6 to prevent cytotoxic lymphocyte-mediated killing
            of tumor cells.
REFERENCE   7  (bases 1 to 3397)
  AUTHORS   Rutitzky LI, Hernandez HJ, Yim YS, Ricklan DE, Finger E, Mohan C,
            Peter I, Wakeland EK and Stadecker MJ.
  TITLE     Enhanced egg-induced immunopathology correlates with high IFN-gamma
            in murine schistosomiasis: identification of two epistatic genetic
            intervals
  JOURNAL   J Immunol 174 (1), 435-440 (2005)
   PUBMED   15611268
REFERENCE   8  (bases 1 to 3397)
  AUTHORS   Kaiserman D, Knaggs S, Scarff KL, Gillard A, Mirza G, Cadman M,
            McKeone R, Denny P, Cooley J, Benarafa C, Remold-O'Donnell E,
            Ragoussis J and Bird PI.
  TITLE     Comparison of human chromosome 6p25 with mouse chromosome 13
            reveals a greatly expanded ov-serpin gene repertoire in the mouse
  JOURNAL   Genomics 79 (3), 349-362 (2002)
   PUBMED   11863365
REFERENCE   9  (bases 1 to 3397)
  AUTHORS   Sun J, Ooms L, Bird CH, Sutton VR, Trapani JA and Bird PI.
  TITLE     A new family of 10 murine ovalbumin serpins includes two homologs
            of proteinase inhibitor 8 and two homologs of the granzyme B
            inhibitor (proteinase inhibitor 9)
  JOURNAL   J Biol Chem 272 (24), 15434-15441 (1997)
   PUBMED   9182575
REFERENCE   10 (bases 1 to 3397)
  AUTHORS   Sun J, Rose JB and Bird P.
  TITLE     Gene structure, chromosomal localization, and expression of the
            murine homologue of human proteinase inhibitor 6 (PI-6) suggests
            divergence of PI-6 from the ovalbumin serpins
  JOURNAL   J Biol Chem 270 (27), 16089-16096 (1995)
   PUBMED   7608171
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK161581.1 and AK035624.1.
            
            On Mar 10, 2006 this sequence version replaced NM_009256.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK161581.1, SRR1660821.173444.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2061              AK161581.1         2-2062
            2062-2320           AK035624.1         2001-2259
            2321-3397           AK161581.1         2322-3398
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 13.79 cM"
     gene            1..3397
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /note="serine (or cysteine) peptidase inhibitor, clade B,
                     member 9"
                     /db_xref="GeneID:20723"
                     /db_xref="MGI:MGI:106603"
     exon            1..155
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     exon            156..333
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1290
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /note="serine (or cysteine) proteinase inhibitor, clade B,
                     member 9; serine protease inhibitor 6"
                     /codon_start=1
                     /product="serpin B9"
                     /protein_id="NP_033282.1"
                     /db_xref="CCDS:CCDS26431.1"
                     /db_xref="GeneID:20723"
                     /db_xref="MGI:MGI:106603"
                     /translation="MNTLSEGNGTFAIHLLKMLCQSNPSKNVCYSPASISSALAMVLL
                     GAKGQTAVQISQALGLNKEEGIHQGFQLLLRKLNKPDRKYSLRVANRLFADKTCEVLQ
                     TFKESSLHFYDSEMEQLSFAEEAEVSRQHINTWVSKQTEGKIPELLSGGSVDSETRLV
                     LINALYFKGKWHQPFNKEYTMDMPFKINKDEKRPVQMMCREDTYNLAYVKEVQAQVLV
                     MPYEGMELSLVVLLPDEGVDLSKVENNLTFEKLTAWMEADFMKSTDVEVFLPKFKLQE
                     DYDMESLFQRLGVVDVFQEDKADLSGMSPERNLCVSKFVHQSVVEINEEGTEAAAASA
                     IIEFCCASSVPTFCADHPFLFFIRHNKANSILFCGRFSSP"
     exon            334..471
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     exon            472..589
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     exon            590..732
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     exon            733..888
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     exon            889..3397
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2293..2298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /note="putative"
     regulatory      3379..3384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /note="putative"
     polyA_site      3397
                     /gene="Serpinb9"
                     /gene_synonym="CAP-3; CAP3; ovalbumin; PI-9; PI9; Spi6"
                     /note="putative"
ORIGIN      
        1 ctcttcctgc tgtggtcgcc agagccagaa gcagcctgtc tccccgcccc tgagcaggag
       61 cacaaaacca gaagtccctg ccttgtacag gcagctctga aagcctactg ggagcatcaa
      121 ggctgcaaac gctggattga gaagccgcaa ctgtgactct gcatcatgaa tactctgtct
      181 gaaggaaatg gcacctttgc catccatctt ttgaagatgc tatgtcaaag caacccttcc
      241 aaaaatgtat gttattctcc tgcgagcatc tcctctgctc tagctatggt tctcttgggt
      301 gcaaagggac agacggcagt ccagatatct caggcacttg gtttgaataa agaggaaggc
      361 atccatcagg gtttccagtt gcttctcagg aagctgaaca agccagacag aaagtactct
      421 cttagagtgg ccaacaggct ctttgcagac aaaacttgtg aagtcctcca aacctttaag
      481 gagtcctctc ttcacttcta tgactcagag atggagcagc tctcctttgc tgaagaagca
      541 gaggtgtcca ggcaacacat aaacacatgg gtctccaaac aaactgaagg taaaattcca
      601 gagttgttgt caggtggctc cgtcgattca gaaaccaggc tggttctcat caatgcctta
      661 tattttaaag gaaagtggca tcaaccattt aacaaagagt acacaatgga catgcccttt
      721 aaaataaaca aggatgagaa aaggccagtg cagatgatgt gtcgtgaaga cacatataac
      781 ctcgcctatg tgaaggaggt gcaggcgcaa gtgctggtga tgccatatga aggaatggag
      841 ctgagcttgg tggttctgct cccagatgag ggtgtggacc tcagcaaggt ggaaaacaat
      901 ctcacttttg agaagttaac agcctggatg gaagcagatt ttatgaagag cactgatgtt
      961 gaggttttcc ttccaaaatt taaactccaa gaggattatg acatggagtc tctgtttcag
     1021 cgcttgggag tggtggatgt cttccaagag gacaaggctg acttatcagg aatgtctcca
     1081 gagagaaacc tgtgtgtgtc caagtttgtt caccagagtg tagtggagat caatgaggaa
     1141 ggcacagagg ctgcagcagc ctctgccatc atagaatttt gctgtgcctc ttctgtccca
     1201 acattctgtg ctgaccaccc cttccttttc ttcatcaggc acaacaaagc aaacagcatc
     1261 ctgttctgtg gcaggttctc atctccataa agacacatat actacacagg gagagttctc
     1321 tcttcagtat ccctaccact cctacagctc tgtcaagatg ggcaagtagg gggaagtcat
     1381 gttctaagat gaagacactt tcttcttgtc agcctgatct tataatgcct gcattcaact
     1441 ctccctgtct tgaatgcatc tatgcccttt accaggttat gtctaatgat gccaaatacc
     1501 ttctgctatg ctattgattg atagcctagc cagtaattta tagccagtta gaactgactt
     1561 gactgtgcaa gaatgctata atggagctag agagaaggca caaacactag gaaaggttgc
     1621 tgtttttgca gaggacacag ggacatttcc caccactcac atggctgctt acaacctctg
     1681 gaaattccag tttctgtcca tgacttgatt cctttctttg gcttctactg gctccagcat
     1741 cctgcacata catgtatcgt cattcagtta cacacaaaca agtaaaattt taaaaataaa
     1801 taaaaattta aagagagagt ctaaaatttt agtaatggtt agataatagc tgctattgtg
     1861 cctttttcag gttttaatgt cattattctt gtgtataaag tcaataattt ataggaaaac
     1921 atcagtgcca tgctgtgatc ttatcttcat cccctttcaa acacattgtt taaccatcaa
     1981 gcagtgactc tccattcttc ctccctttgg acatataacc accaatcttc tctctgtctc
     2041 tgtagcttta tctattttgg acattttata tagactgcct ctttgctatc ttgataatgt
     2101 cctttgatgc atacagattg ctttttttcc agtgatgttc tccttattta atactgttct
     2161 gatcatgagg tatgcatgct gcagctaaga atctcactac taaagtcaag tatagaaata
     2221 actgttctta tgttttcctc caaggccttc atggattata ccttatatat ctaggtcact
     2281 gttccagttt gaattaaatt ccatatgtgg tgtggtgttg gagcctgatg tgattccttt
     2341 atttgtgaac atccagttgt cgctgtactt gtaattgctt tgaatcatga ggtaatgacc
     2401 aatgaatagg aaaaataatg ttacaactag tggggtagga tagaagttcc tcagtacata
     2461 tattgaacat taacacaagt gtgaaataaa aatatcagaa atgagcagta aacaaagaag
     2521 gagagattaa acgttctgta atcagggtcc tcagaaatga ctcaggggct gaagatgctt
     2581 gctaccctgc ctgacaactt gagttctatt tctggaacag acatggtgga agaagaaaac
     2641 tgactctagc aagttgtcct ctgacctcac catgagcatg atgcacaaat aaatatccct
     2701 gttaatcaga tataattatg cagtaagtta ataatcagaa aaaatacaca tataaaaacc
     2761 aattccttat caacaagtta atcttgctag gaagagccag gaaaaagagt aataggggac
     2821 catgtgatcc taaaagggat aaagctaagc caggatatac tcttatacat caacatgtgt
     2881 cctggtttcc agaaggttca atttgaggac atatcccaat gatgtcatta ctgaagtcca
     2941 caaaaagaca gtgactatga tctctgtagc catatttata gcagcaaata atggatccag
     3001 gtgtcaatct catacagtaa acaaagaagt catgttgtac acttaatact gaatggaagt
     3061 tagaaagttg taccagatag tttttatatt aatgtgatac aaggtagagt cactggagag
     3121 gaaagagcct caattaagaa atgtctccat atgctctgga gcattaatgt gagaaggcta
     3181 ggcccattgt atgtgagaca atccctatgc aggtggtcct ctgattataa ggaagcaggc
     3241 tgaacaaacc aggaggagca agccagtaag cagcattcct ctgtggcctc tgcatcagct
     3301 cctgcctcca gtttcttgcc ccgttggcat tcttctgact tccttcattg atgaacagtg
     3361 atgtaaacgt ataagccaaa taaacctttt ctacccc
//
