LOCUS       NM_009254               1432 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade B,
            member 6a (Serpinb6a), transcript variant 2, mRNA.
ACCESSION   NM_009254
VERSION     NM_009254.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Cheng,C., Qi,J., Zhang,L., Li,H., Lu,J., Li,S., Zhang,Z., Qiu,Y.,
            Zhang,C., Jiang,L., Yu,C., Gao,X., Bird,P.I. and Chai,R.
  TITLE     Absence of Serpinb6a causes progressive hair cell apoptosis and
            hearing loss in mice
  JOURNAL   J Genet Genomics 50 (2), 122-125 (2023)
   PUBMED   36087923
  REMARK    GeneRIF: Absence of Serpinb6a causes progressive hair cell
            apoptosis and hearing loss in mice.
            Erratum:[J Genet Genomics. 2023 Jun;50(6):458. doi:
            10.1016/j.jgg.2023.04.011.. PMID: 37330250]
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Tan,J., Kaiserman,D., O'Leary,S.J. and Bird,P.I.
  TITLE     Mice heterozygous for the Serpinb6a null mutation show deficits in
            central auditory function after acoustic trauma
  JOURNAL   Neuroreport 32 (16), 1287-1292 (2021)
   PUBMED   34554936
  REMARK    GeneRIF: Mice heterozygous for the Serpinb6a null mutation show
            deficits in central auditory function after acoustic trauma.
REFERENCE   3  (bases 1 to 1432)
  AUTHORS   Burgener,S.S., Leborgne,N.G.F., Snipas,S.J., Salvesen,G.S.,
            Bird,P.I. and Benarafa,C.
  TITLE     Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed
            Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven
            Inflammation
  JOURNAL   Cell Rep 27 (12), 3646-3656 (2019)
   PUBMED   31216481
REFERENCE   4  (bases 1 to 1432)
  AUTHORS   Nakata,H., Yamamoto,M., Kumchantuek,T., Adhapanyawanich,K.,
            Nishiuchi,T. and Iseki,S.
  TITLE     Synthesis, localization and possible function of serine (or
            cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in
            mouse submandibular gland
  JOURNAL   Cell Tissue Res 369 (3), 513-526 (2017)
   PUBMED   28455723
  REMARK    GeneRIF: Serpinb6a mRNA expression was more abundant in the male
            than in the female submandibular gland.
REFERENCE   5  (bases 1 to 1432)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1432)
  AUTHORS   Dai,G., Chapman,B.M., Liu,B., Orwig,K.E., Wang,D., White,R.A.,
            Preuett,B. and Soares,M.J.
  TITLE     A new member of the mouse prolactin (PRL)-like protein-C subfamily,
            PRL-like protein-C alpha: structure and expression
  JOURNAL   Endocrinology 139 (12), 5157-5163 (1998)
   PUBMED   9832456
REFERENCE   7  (bases 1 to 1432)
  AUTHORS   Nakaya,N., Nishibori,M., Wang,Z., Sakiyama,J. and Saeki,K.
  TITLE     The expression and localization of serine proteinase inhibitor PI-6
            mRNA in developmental and ischemic mouse brain
  JOURNAL   Neurosci Res 32 (3), 221-230 (1998)
   PUBMED   9875564
REFERENCE   8  (bases 1 to 1432)
  AUTHORS   Sun,J., Ooms,L., Bird,C.H., Sutton,V.R., Trapani,J.A. and Bird,P.I.
  TITLE     A new family of 10 murine ovalbumin serpins includes two homologs
            of proteinase inhibitor 8 and two homologs of the granzyme B
            inhibitor (proteinase inhibitor 9)
  JOURNAL   J Biol Chem 272 (24), 15434-15441 (1997)
   PUBMED   9182575
REFERENCE   9  (bases 1 to 1432)
  AUTHORS   Safaei,R.
  TITLE     A target of the HoxB5 gene from the mouse nervous system
  JOURNAL   Brain Res Dev Brain Res 100 (1), 5-12 (1997)
   PUBMED   9174240
REFERENCE   10 (bases 1 to 1432)
  AUTHORS   Sun,J., Rose,J.B. and Bird,P.
  TITLE     Gene structure, chromosomal localization, and expression of the
            murine homologue of human proteinase inhibitor 6 (PI-6) suggests
            divergence of PI-6 from the ovalbumin serpins
  JOURNAL   J Biol Chem 270 (27), 16089-16096 (1995)
   PUBMED   7608171
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL450406.17.
            
            On Aug 12, 2009 this sequence version replaced NM_009254.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and uses
            a downstream, in-frame start site, compared to variant 1. The
            encoded isoform (b) has a shorter N-terminus, compared to isoform
            a. Variants 2, 3 and 4 encode the same isoform.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057950.1, U25844.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AL450406.17        209030-209191       c
            163-337             AL450406.17        204580-204754       c
            338-490             AL450406.17        203133-203285       c
            491-608             AL450406.17        198420-198537       c
            609-751             AL450406.17        196034-196176       c
            752-907             AL450406.17        191849-192004       c
            908-1432            AL450406.17        191026-191550       c
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 14.0 cM"
     gene            1..1432
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /note="serine (or cysteine) peptidase inhibitor, clade B,
                     member 6a"
                     /db_xref="GeneID:20719"
                     /db_xref="MGI:MGI:103123"
     exon            1..162
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     exon            163..337
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1309
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /note="isoform b is encoded by transcript variant 2;
                     serine (or cysteine) proteinase inhibitor, clade B, member
                     6a; serpin B6; placental thrombin inhibitor; serine (or
                     cysteine) proteinase inhibitor, clade B (ovalbumin),
                     member 6; serine protease inhibitor 3"
                     /codon_start=1
                     /product="serpin B6 isoform b"
                     /protein_id="NP_033280.1"
                     /db_xref="CCDS:CCDS26441.1"
                     /db_xref="GeneID:20719"
                     /db_xref="MGI:MGI:103123"
                     /translation="MDPLQEANGTFALNLLKILGEDSSKNVFLSPMSISSALAMVFMG
                     AKGTTASQMAQALALDKCSGNGGGDVHQGFQSLLTEVNKTGTQYLLRTANRLFGDKTC
                     DLLASFKDSCLKFYEAELEELDFQGATEESRQHINTWVAKKTEDKIKEVLSPGTVNSD
                     TSLVLVNAIYFKGNWEKQFNKEHTREMPFKVSKNEEKPVQMMFKKSTFKMTYIGEIFT
                     KILLLPYVSSELNMIIMLPDEHVELSTVEKEVTYEKFIEWTRLDKMDEEEVEVFLPKF
                     KLEENYNMNDALYKLGMTDAFGGRADFSGMSSKQGLFLSKVVHKAFVEVNEEGTEAAA
                     ATAGMMTVRCMRFTPRFCADHPFLFFIHHVKTNGILFCGRFSSP"
     exon            338..490
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     exon            491..608
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     exon            609..751
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     exon            752..907
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1432
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1400..1405
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /note="hexamer: AATAAA"
     polyA_site      1423
                     /gene="Serpinb6a"
                     /gene_synonym="4930482L21Rik; D330015H01Rik; ovalbumin;
                     PI-6; Serpinb6; Spi3"
                     /note="major polyA site"
ORIGIN      
        1 tctcttcctt ttcctactcg ggagcggccg caccctggcc ttcagactcc tcccgactct
       61 ccgcccccca taagtattta aggcctcggg cgcggttggg gcctcctcat ctcttctctg
      121 cttgctacca gtccggaaat cgagaatcta ggagaatcta gggctcacca tcatggatcc
      181 tctacaggaa gcaaatggca cctttgcctt aaaccttttg aaaatactgg gtgaagacag
      241 ctcaaaaaat gtatttttgt cacccatgag catatcctca gccctggcta tggtcttcat
      301 gggggcaaaa ggaaccactg ctagccagat ggctcaggca cttgctttgg ataaatgcag
      361 cggcaatgga ggtggagatg tccaccaggg cttccagtcc cttctcaccg aagtgaacaa
      421 aactggcaca cagtacttgc tcagaacagc caacaggctc ttcggggata agacttgtga
      481 tcttttagcg tcttttaaag attcctgcct caagttctat gaagcagagt tggaagagct
      541 ggactttcag ggtgctacag aggagtcccg acagcacatc aacacctggg tagccaaaaa
      601 gacagaagat aaaatcaaag aggtgctgtc tccaggtaca gtgaattctg atacatcgct
      661 agtccttgtg aatgccatct acttcaaagg aaactgggag aagcagttta acaaagagca
      721 taccagggag atgccattca aagtcagcaa gaatgaggag aaacctgtgc aaatgatgtt
      781 taagaagtct acctttaaga tgacctatat tggagagata ttcactaaga ttctgttgct
      841 tccctatgtc agcagtgagc tgaacatgat catcatgctt ccagatgagc acgttgaact
      901 gagtacagtg gaaaaggaag taacttacga gaaatttata gagtggacaa ggctggacaa
      961 gatggacgaa gaagaggtag aagtatttct cccaaagttt aagctggagg agaattacaa
     1021 catgaacgat gccctctaca agttgggcat gactgatgcc tttggcggca gggcagactt
     1081 ttctggaatg tcttccaagc aaggcttgtt tctgtctaag gttgtgcata aggcctttgt
     1141 ggaggttaat gaggagggca cagaggctgc agctgctaca gctggcatga tgacggtgag
     1201 gtgcatgaga ttcactcccc gcttctgtgc cgaccacccc ttccttttct tcattcacca
     1261 tgttaagacc aatggaattc tgttctgtgg ccggttctcc tctccctgag caaagggtat
     1321 tcctgtcagt ctttgaccct ctctccatgg tttgctgtaa cccaagtgac cttatccata
     1381 agtgcaatgg caattatgaa ataaagggct ttatggcacc ccaccttcag aa
//
