LOCUS       NM_009257               2570 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade B,
            member 5 (Serpinb5), transcript variant 2, mRNA.
ACCESSION   NM_009257
VERSION     NM_009257.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Kim,S.H., Mitchel,J.A., McGill,M., Cremona,T.P., Baek,J.W.,
            Kasahara,D.I., Anathy,V., Israel,E. and Park,J.A.
  TITLE     Increased extracellular maspin levels after mechanical compression
            in vitro or allergen challenge in vivo
  JOURNAL   J Allergy Clin Immunol 144 (4), 1116-1118 (2019)
   PUBMED   31228474
  REMARK    GeneRIF: This is the first study to recognize the potential
            implication of maspin in the pathophysiology of asthma
REFERENCE   3  (bases 1 to 2570)
  AUTHORS   Mahajan,N., Hoover,B., Rajendram,M., Shi,H.Y., Kawasaki,K.,
            Weibel,D.B. and Zhang,M.
  TITLE     Maspin binds to cardiolipin in mitochondria and triggers apoptosis
  JOURNAL   FASEB J 33 (5), 6354-6364 (2019)
   PUBMED   30786218
  REMARK    GeneRIF: model of apoptosis in which maspin binds to cardiolipin,
            displaces cytochrome c from the membrane, and facilitates its
            release to the cytoplasm.
REFERENCE   4  (bases 1 to 2570)
  AUTHORS   Zhang,M., Hendrix,M.J.C., Pemberton,P.A., Sakr,W.A. and Sheng,S.
  TITLE     An Essential Role of Maspin in Embryogenesis and Tumor
            Suppression-Response
  JOURNAL   Cancer Res 77 (18), 5208-5210 (2017)
   PUBMED   28877938
REFERENCE   5  (bases 1 to 2570)
  AUTHORS   Dzinic,S.H., Bernardo,M.M., Li,X., Fernandez-Valdivia,R., Ho,Y.S.,
            Mi,Q.S., Bandyopadhyay,S., Lonardo,F., Vranic,S., Oliveira,D.S.,
            Bonfil,R.D., Dyson,G., Chen,K., Omerovic,A., Sheng,X., Han,X.,
            Wu,D., Bi,X., Cabaravdic,D., Jakupovic,U., Wahba,M., Pang,A.,
            Harajli,D., Sakr,W.A. and Sheng,S.
  TITLE     An Essential Role of Maspin in Embryogenesis and Tumor Suppression
  JOURNAL   Cancer Res 77 (4), 886-896 (2017)
   PUBMED   27923833
  REMARK    GeneRIF: maspin is a powerful context-dependent tumor suppressor.
REFERENCE   6  (bases 1 to 2570)
  AUTHORS   Kaiserman,D., Knaggs,S., Scarff,K.L., Gillard,A., Mirza,G.,
            Cadman,M., McKeone,R., Denny,P., Cooley,J., Benarafa,C.,
            Remold-O'Donnell,E., Ragoussis,J. and Bird,P.I.
  TITLE     Comparison of human chromosome 6p25 with mouse chromosome 13
            reveals a greatly expanded ov-serpin gene repertoire in the mouse
  JOURNAL   Genomics 79 (3), 349-362 (2002)
   PUBMED   11863365
REFERENCE   7  (bases 1 to 2570)
  AUTHORS   Ngamkitidechakul,C., Burke,J.M., O'Brien,W.J. and Twining,S.S.
  TITLE     Maspin: synthesis by human cornea and regulation of in vitro
            stromal cell adhesion to extracellular matrix
  JOURNAL   Invest Ophthalmol Vis Sci 42 (13), 3135-3141 (2001)
   PUBMED   11726614
REFERENCE   8  (bases 1 to 2570)
  AUTHORS   Zhang,M., Magit,D., Botteri,F., Shi,H.Y., He,K., Li,M., Furth,P.
            and Sager,R.
  TITLE     Maspin plays an important role in mammary gland development
  JOURNAL   Dev Biol 215 (2), 278-287 (1999)
   PUBMED   10545237
REFERENCE   9  (bases 1 to 2570)
  AUTHORS   Sheng,S., Truong,B., Fredrickson,D., Wu,R., Pardee,A.B. and
            Sager,R.
  TITLE     Tissue-type plasminogen activator is a target of the tumor
            suppressor gene maspin
  JOURNAL   Proc Natl Acad Sci U S A 95 (2), 499-504 (1998)
   PUBMED   9435220
REFERENCE   10 (bases 1 to 2570)
  AUTHORS   Zhang,M., Sheng,S., Maass,N. and Sager,R.
  TITLE     mMaspin: the mouse homolog of a human tumor suppressor gene
            inhibits mammary tumor invasion and motility
  JOURNAL   Mol Med 3 (1), 49-59 (1997)
   PUBMED   9132279
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK136532.1, AC025047.17 and AC163626.5.
            
            On Feb 15, 2018 this sequence version replaced NM_009257.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005434.1, AK136532.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1322              AK136532.1         2-1323
            1323-1986           AC025047.17        1-664               c
            1987-2570           AC163626.5         76359-76942
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 50.34 cM"
     gene            1..2570
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="serine (or cysteine) peptidase inhibitor, clade B,
                     member 5"
                     /db_xref="GeneID:20724"
                     /db_xref="MGI:MGI:109579"
     exon            1..86
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     exon            87..255
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1215
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="isoform a is encoded by transcript variant 2;
                     serine (or cysteine) proteinase inhibitor, clade B, member
                     5; serpin B5; protease inhibitor 5; peptidase inhibitor 5;
                     serine (or cysteine) proteinase inhibitor, clade B
                     (ovalbumin), member 5; serine protease inhibitor 7"
                     /codon_start=1
                     /product="serpin B5 isoform a"
                     /protein_id="NP_033283.1"
                     /db_xref="CCDS:CCDS35684.1"
                     /db_xref="GeneID:20724"
                     /db_xref="MGI:MGI:109579"
                     /translation="MDALRLANSAFAVDLFKQLCERDPAGNILFSPICLSTSLSLAQV
                     GTKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLVKRLYIDKSLNPST
                     EFISSTKRPYAKELETVDFKDKLEETKGQINSSIKELTDGHFEDILSENSISDQTKIL
                     VVNAAYFVGKWMKKFPESETKECPFRISKTDTKPVQMMNLEATFCLGNIDDISCKIIE
                     LPFQNKHLSMLIVLPKDVEDESTGLEKIEQQLNPETLLQWTNPSTMANAKVKLSLPKF
                     KVEKMIDPKASLESLGLKSLFNESTSDFSGMSETKGVSLSNVIHRVCLEITEDGGESI
                     EVPGSRILQHKDEFNADHPFIYIIRHNKTRNIIFFGKFCSP"
     exon            256..393
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     exon            394..511
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     exon            512..654
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     exon            655..822
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     exon            823..2570
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1302..1307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="hexamer: AATAAA"
     polyA_site      1331
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="major polyA site"
     regulatory      2551..2556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="hexamer: AATAAA"
     polyA_site      2570
                     /gene="Serpinb5"
                     /gene_synonym="1110036M19Rik; Maspin; ovalbumin; PI-5;
                     Spi7"
                     /note="major polyA site"
ORIGIN      
        1 agagagcaca gctttcactg cctctgagct ccttcggctt tttcccccct tttgcatctt
       61 gtttcccggg atacctgcaa ctcaaggatg gatgccctga gactggcaaa ttcagctttt
      121 gctgttgact tgttcaaaca actatgtgaa agggacccag caggaaacat tctcttctct
      181 ccaatatgcc tctctacttc tctgtccctt gcgcaagtgg gcaccaaagg cgacacagca
      241 aatgaaattg gacaggtcct tcattttgag aatgtcaaag atgtaccctt tgggtttcaa
      301 acagtcactt ctgatgttaa taagctcagt tctttttact ctttgaaact tgtcaagcga
      361 ctctacatag acaaatctct gaacccttct acagaattta tcagttctac caaaagacca
      421 tatgcaaaag aattggaaac tgttgacttc aaagacaaac tggaagaaac gaaaggtcaa
      481 attaacagct ccattaagga gctcacagat ggccactttg aggacatttt gtcagagaac
      541 agtataagtg accagaccaa aatccttgtg gttaatgctg cctactttgt tggaaagtgg
      601 atgaagaaat ttccggaatc agaaacaaaa gaatgtcctt tcagaatcag caagacagac
      661 accaaacccg tacaaatgat gaatcttgag gccactttct gcttgggtaa cattgatgac
      721 atcagctgta agatcataga acttcctttc cagaataagc atctgagtat gctcattgtg
      781 ctccccaagg acgtggagga tgagtccaca ggcctggaga agattgaaca gcaactcaac
      841 ccagaaacat tgttacagtg gaccaacccc agtaccatgg ccaatgccaa agtcaaactt
      901 tccctcccaa agtttaaggt agaaaagatg attgatccca aggctagtct ggaaagccta
      961 gggctgaaaa gtctcttcaa tgaaagtaca tcggatttct ctggaatgtc agagaccaag
     1021 ggagtgtccc tgtcaaatgt gattcataga gtatgcctag aaataaccga agatggtggt
     1081 gagtccatcg aggtgccagg gtcccggatc ttacagcaca aggatgaatt caatgctgac
     1141 catccattta tttatatcat tagacacaac aaaactcgaa acatcatttt ctttggcaaa
     1201 ttctgttctc cttagctggc agggccttgc caagtctcag ggaacttgtc tgtagtcgca
     1261 gagctctgta aactttgtat ccagacaatc actttctata caataaattg taaatgttgc
     1321 tgaatcagga agcagtccac atttgtcata tgtaaccttc acactaacag acaccccttt
     1381 tcctaattct tccttgtttt cctttgtcaa agaaaacagc gaatacttgt tgggaaaggg
     1441 aatcacttta gaaaatatgt gctcttcatt tgtcaaagca ttcagagcta tggataaaaa
     1501 tgcagtcttc aagggaggaa cttctatagg gatgatgtgg gggacattat tctcagggct
     1561 ctgcattact gagataaaga atgttctaga gatctccact tccttcaact cttaagaaca
     1621 agagtatagg gttcaccaag taccttttta aagtgaattt gtgtacatgt cacaatactg
     1681 taggtctaga agtctccatg ttaaacctta agaaacagtt ttcttcaata tcctaattgg
     1741 agattataga aagatgacat gttgtaaata ctatatatca tggaactcca caaatagcat
     1801 atggactatc accctatcaa ccaatacctt aatatttgtt ccataaatat agactattca
     1861 cacaaaacaa gtacataaag atagttcata agtgatcaat tttttccctg aaaaatgagt
     1921 atgttatcag ctcatggaaa cactttgaag aacattctag atcttggagt gttggataaa
     1981 gaattctggg cctattttag ctgaagacta agaccctaag ggaaagctca gcttttacaa
     2041 aagttggctt tcatttttcc tagatctccc atgtggtcat ttgggcaatg ttgttgctgg
     2101 caggcttcta accagatcac aaggtccctg acatctccag cgccagcaac ttaaaatcta
     2161 caggaagtct cgtgatgcta agagttttgt ggccattaaa ggaactttcc ttttttgtct
     2221 aatgcggcag tggagaaaga gaagggatct caaaccttca gaaaatacaa gcaacactat
     2281 ttaggtacac atagttcttt ggcatatatt ttctcagttt acaaacctaa cgacttcaga
     2341 taacagtgtg tagtctacat aaataattta taaaatggtt ctggtaagtc agttatgttg
     2401 caacctaatt ttaaagttgc caccaaaagg aagtatttgc tgcagttttt cttatgtgtc
     2461 tttccttacc caatttcttg ccatttattg taaatagggt ttttttttcc tccaaaggtt
     2521 caatggtgac ttcttttctg tgctattgat aataaaatat tattgaatta
//
