LOCUS       NM_009252               2085 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade A,
            member 3N (Serpina3n), mRNA.
ACCESSION   NM_009252 XM_011244046 XM_036157281
VERSION     NM_009252.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Sun,Q., Chen,W., Wu,R., Tao,B., Wang,P., Sun,B., Alvarez,J.F.,
            Ma,F., Galindo,D.C., Maroney,S.P., Saviola,A.J., Hansen,K.C., Li,S.
            and Deb,A.
  TITLE     Serine protease inhibitor, SerpinA3n, regulates cardiac remodelling
            after myocardial infarction
  JOURNAL   Cardiovasc Res 120 (8), 943-953 (2024)
   PUBMED   38666458
  REMARK    GeneRIF: Serine protease inhibitor, SerpinA3n, regulates cardiac
            remodelling after myocardial infarction.
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Han,X., Lei,Q., Liu,H., Zhang,T. and Gou,X.
  TITLE     SerpinA3N Regulates the Secretory Phenotype of Mouse Senescent
            Astrocytes Contributing to Neurodegeneration
  JOURNAL   J Gerontol A Biol Sci Med Sci 79 (4) (2024)
   PUBMED   38175667
  REMARK    GeneRIF: SerpinA3N Regulates the Secretory Phenotype of Mouse
            Senescent Astrocytes Contributing to Neurodegeneration.
REFERENCE   3  (bases 1 to 2085)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2085)
  AUTHORS   Liu,C., Zhao,X.M., Wang,Q., Du,T.T., Zhang,M.X., Wang,H.Z.,
            Li,R.P., Liang,K., Gao,Y., Zhou,S.Y., Xue,T., Zhang,J.G., Han,C.L.,
            Shi,L., Zhang,L.W. and Meng,F.G.
  TITLE     Astrocyte-derived SerpinA3N promotes neuroinflammation and
            epileptic seizures by activating the NF-kappaB signaling pathway in
            mice with temporal lobe epilepsy
  JOURNAL   J Neuroinflammation 20 (1), 161 (2023)
   PUBMED   37422673
  REMARK    GeneRIF: Astrocyte-derived SerpinA3N promotes neuroinflammation and
            epileptic seizures by activating the NF-kappaB signaling pathway in
            mice with temporal lobe epilepsy.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2085)
  AUTHORS   Tran,M., Mostofa,G., Picard,M., Wu,J., Wang,L. and Shin,D.J.
  TITLE     SerpinA3N deficiency attenuates steatosis and enhances insulin
            signaling in male mice
  JOURNAL   J Endocrinol 256 (3), e220073 (2023)
   PUBMED   36625462
  REMARK    GeneRIF: SerpinA3N deficiency attenuates steatosis and enhances
            insulin signaling in male mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2085)
  AUTHORS   Winkler,I.G., Hendy,J., Coughlin,P., Horvath,A. and Levesque,J.P.
  TITLE     Serine protease inhibitors serpina1 and serpina3 are down-regulated
            in bone marrow during hematopoietic progenitor mobilization
  JOURNAL   J Exp Med 201 (7), 1077-1088 (2005)
   PUBMED   15795238
REFERENCE   7  (bases 1 to 2085)
  AUTHORS   Horvath,A.J., Forsyth,S.L. and Coughlin,P.B.
  TITLE     Expression patterns of murine antichymotrypsin-like genes reflect
            evolutionary divergence at the Serpina3 locus
  JOURNAL   J Mol Evol 59 (4), 488-497 (2004)
   PUBMED   15638460
REFERENCE   8  (bases 1 to 2085)
  AUTHORS   Yoshida,S., Mears,A.J., Friedman,J.S., Carter,T., He,S., Oh,E.,
            Jing,Y., Farjo,R., Fleury,G., Barlow,C., Hero,A.O. and Swaroop,A.
  TITLE     Expression profiling of the developing and mature Nrl-/- mouse
            retina: identification of retinal disease candidates and
            transcriptional regulatory targets of Nrl
  JOURNAL   Hum Mol Genet 13 (14), 1487-1503 (2004)
   PUBMED   15163632
REFERENCE   9  (bases 1 to 2085)
  AUTHORS   Forsyth,S., Horvath,A. and Coughlin,P.
  TITLE     A review and comparison of the murine alpha1-antitrypsin and
            alpha1-antichymotrypsin multigene clusters with the human clade A
            serpins
  JOURNAL   Genomics 81 (3), 336-345 (2003)
   PUBMED   12659817
  REMARK    Review article
REFERENCE   10 (bases 1 to 2085)
  AUTHORS   Inglis,J.D., Lee,M., Davidson,D.R. and Hill,R.E.
  TITLE     Isolation of two cDNAs encoding novel alpha 1-antichymotrypsin-like
            proteins in a murine chondrocytic cell line
  JOURNAL   Gene 106 (2), 213-220 (1991)
   PUBMED   1718822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117194.3.
            
            On or before Jun 20, 2023 this sequence version replaced
            XM_011244046.4, XM_036157281.1, NM_009252.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013651.1, SRR7345562.1215495.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                AC117194.3         69735-69822         c
            89-742              AC117194.3         67223-67876         c
            743-1016            AC117194.3         65130-65403         c
            1017-1167           AC117194.3         64046-64196         c
            1168-2085           AC117194.3         62201-63118         c
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 54.17 cM"
     gene            1..2085
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /note="serine (or cysteine) peptidase inhibitor, clade A,
                     member 3N"
                     /db_xref="GeneID:20716"
                     /db_xref="MGI:MGI:105045"
     exon            1..88
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /inference="alignment:Splign:2.1.0"
     exon            89..742
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..1362
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /note="serine (or cysteine) proteinase inhibitor, clade A,
                     member 3N; serine protease inhibitor A3N; serpin A3N;
                     serine protease inhibitor 2-2; antitrypsin; alpha-1
                     antiproteinase"
                     /codon_start=1
                     /product="serine protease inhibitor A3N precursor"
                     /protein_id="NP_033278.2"
                     /db_xref="CCDS:CCDS26152.1"
                     /db_xref="GeneID:20716"
                     /db_xref="MGI:MGI:105045"
                     /translation="MAFIAALGLLMAGICPAVLCFPDGTLGMDAAVQEDHDNGTQLDS
                     LTLASINTDFAFSLYKELVLKNPDKNIVFSPLSISAALAVMSLGAKGNTLEEILEGLK
                     FNLTETSEADIHQGFGHLLQRLNQPKDQVQISTGSALFIEKRQQILTEFQEKARALYQ
                     AEAFTADFQQPRQAKKLINDYVRKQTQGMIKELVSDLDKRTLMVLVNYIYFKAKWKVP
                     FDPLDTFKSEFYAGKRRPVIVPMMSMEDLTTPYFRDEELFCTVVELKYTGNASAMFIL
                     PDQGKMQQVEASLQPETLRKWKNSLKPRMIDELHLPKFSISTDYSLEDVLSKLGIREV
                     FSTQADLSAITGTKDLRVSQVVHKAVLDVAETGTEAAAATGVKFVPMSAKLYPLTVYF
                     NRPFLIMIFDTETEIAPFIAKIANPK"
     exon            743..1016
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1167
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..2085
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2043..2048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /note="hexamer: AATAAA"
     polyA_site      2085
                     /gene="Serpina3n"
                     /gene_synonym="Spi2-2; Spi2.2; Spi2/eb.4"
                     /note="major polyA site"
ORIGIN      
        1 ggggacaaca tgccaggcaa gcggcaaccc tgaacatcgg gagtcagcta tcacagaggc
       61 tggcagctgg ctggtttcag ctctgtagac tgcagaacac agaagatggc cttcattgca
      121 gctctggggc tcttgatggc tgggatctgc cctgctgtcc tctgcttccc agatggcacg
      181 ttgggaatgg atgctgcagt ccaagaagac catgacaatg ggacacaact ggacagtctc
      241 acattggcct ccatcaacac tgactttgcc ttcagcctct acaaggagct ggttttgaag
      301 aatccagata aaaatattgt cttctcccca cttagcatct cagcggcctt ggccgtcatg
      361 tccctgggag caaagggcaa caccctggaa gagattctag aaggtctcaa gttcaatctt
      421 acagagacct ctgaggcaga catccaccag ggctttgggc acctcctaca gaggctcaac
      481 cagccaaagg accaggtaca gatcagcacg ggtagtgccc tgtttattga aaagcgccag
      541 cagatcctga cagaattcca ggagaaggca agggctctgt accaggctga ggccttcaca
      601 gcagacttcc agcagcctcg tcaggccaaa aagctcatca atgactatgt gaggaaacag
      661 acccagggga tgatcaagga actggtctca gacctggata aaaggacatt gatggtgctg
      721 gtgaattata tctactttaa agccaaatgg aaggtgccct ttgaccctct tgacacgttc
      781 aagtctgagt tctacgcggg caagaggagg cccgtgatag tgcccatgat gagcatggag
      841 gacctgacca caccctactt ccgagatgag gagcttttct gcactgtggt ggagctgaag
      901 tacacaggaa atgccagtgc catgttcatc ctccctgacc agggcaagat gcagcaggtg
      961 gaagccagct tgcaaccaga gaccctgagg aagtggaaga attctctgaa acccaggatg
     1021 atagatgagc tccacctgcc caagttctcc atctccaccg actacagcct ggaggatgtc
     1081 ctttcaaagc tgggcatcag ggaagtcttc tccacacagg ctgacctgtc tgcaatcaca
     1141 ggaaccaagg atctgagagt ctctcaggtg gtccacaagg ctgtgctgga cgtggctgag
     1201 acaggcacag aagcagctgc tgccactgga gtcaaatttg tcccaatgtc tgcgaaactg
     1261 taccctctga ctgtatattt caatcggcct ttcctgataa tgatctttga cacagaaact
     1321 gaaattgccc cctttatagc caagatagcc aaccccaaat gagactagaa ctccccaagt
     1381 gttgacgctt cttcccggga gccaggcatt gagcctgtct gtgggtctcc atgtgcattt
     1441 tggcttccat gctctgcttg gccttggcat gcctggatta gatagtgact aactgtgtta
     1501 taacctcatg cacagacatc cctgtgggaa gtcagtgcca tgctcccaga cttcttggta
     1561 gcactagccc atgttcctga gcctgaaatt tgtcttgtcc cctacccctg ctctctccct
     1621 gtatctgcct ccacccaaaa gcctgggccc catcaagtaa gctcagtccc agtttagact
     1681 ctggttatgt ctcccttcag ccttgctgtc ttgatgggac tgtgcaacct tacaggccaa
     1741 cccatatgga ccaagaggaa agcttgctgc cccgtgtcta tctgcagcat ctaggactac
     1801 ttggtgccca gtctgcctca ctctttcact tctccaggct ttgcctccag cactgccccc
     1861 ctgcaggatc tcctcacttt gtccacatct ggcacagagt tagagccctc acttctgcag
     1921 ctgcatgggg cctgtgggtc agagcagctc ctttcccctg gcactcctac ttagaacaaa
     1981 gtagcctttc ttttagttcc cagctgacca acctcacaca aaagaggaac accaccaacc
     2041 agaataaaga ggtaggagca aaagatcaat aaacatataa ctgca
//
