LOCUS       NM_009253               1660 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade A,
            member 3M (Serpina3m), transcript variant 1, mRNA.
ACCESSION   NM_009253
VERSION     NM_009253.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Heit,C., Jackson,B.C., McAndrews,M., Wright,M.W., Thompson,D.C.,
            Silverman,G.A., Nebert,D.W. and Vasiliou,V.
  TITLE     Update of the human and mouse SERPIN gene superfamily
  JOURNAL   Hum Genomics 7 (1), 22 (2013)
   PUBMED   24172014
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Ghesquiere,B., Van Damme,J., Martens,L., Vandekerckhove,J. and
            Gevaert,K.
  TITLE     Proteome-wide characterization of N-glycosylation events by
            diagonal chromatography
  JOURNAL   J Proteome Res 5 (9), 2438-2447 (2006)
   PUBMED   16944957
REFERENCE   3  (bases 1 to 1660)
  AUTHORS   Winkler,I.G., Hendy,J., Coughlin,P., Horvath,A. and Levesque,J.P.
  TITLE     Serine protease inhibitors serpina1 and serpina3 are down-regulated
            in bone marrow during hematopoietic progenitor mobilization
  JOURNAL   J Exp Med 201 (7), 1077-1088 (2005)
   PUBMED   15795238
REFERENCE   4  (bases 1 to 1660)
  AUTHORS   Horvath,A.J., Forsyth,S.L. and Coughlin,P.B.
  TITLE     Expression patterns of murine antichymotrypsin-like genes reflect
            evolutionary divergence at the Serpina3 locus
  JOURNAL   J Mol Evol 59 (4), 488-497 (2004)
   PUBMED   15638460
REFERENCE   5  (bases 1 to 1660)
  AUTHORS   Forsyth,S., Horvath,A. and Coughlin,P.
  TITLE     A review and comparison of the murine alpha1-antitrypsin and
            alpha1-antichymotrypsin multigene clusters with the human clade A
            serpins
  JOURNAL   Genomics 81 (3), 336-345 (2003)
   PUBMED   12659817
  REMARK    Review article
REFERENCE   6  (bases 1 to 1660)
  AUTHORS   Ohkubo,K., Ogata,S., Misumi,Y., Takami,N., Sinohara,H. and
            Ikehara,Y.
  TITLE     Cloning, structure and expression of cDNA for mouse contrapsin and
            a related protein
  JOURNAL   Biochem J 276 (Pt 2) (Pt 2), 337-342 (1991)
   PUBMED   2049065
REFERENCE   7  (bases 1 to 1660)
  AUTHORS   Inglis,J.D. and Hill,R.E.
  TITLE     The murine Spi-2 proteinase inhibitor locus: a multigene family
            with a hypervariable reactive site domain
  JOURNAL   EMBO J 10 (2), 255-261 (1991)
   PUBMED   1991447
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117194.3.
            
            On Jul 25, 2022 this sequence version replaced NM_009253.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X55148.1, BC011158.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AC117194.3         89307-89365         c
            60-708              AC117194.3         86818-87466         c
            709-982             AC117194.3         84801-85074         c
            983-1133            AC117194.3         83738-83888         c
            1134-1660           AC117194.3         82271-82797         c
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 54.12 cM"
     gene            1..1660
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /note="serine (or cysteine) peptidase inhibitor, clade A,
                     member 3M"
                     /db_xref="GeneID:20717"
                     /db_xref="MGI:MGI:98378"
     exon            1..59
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /inference="alignment:Splign:2.1.0"
     exon            60..708
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1328
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; serine (or cysteine) proteinase inhibitor,
                     clade A, member 3M; serine protease inhibitor A3M; serpin
                     A3M; serine protease inhibitor-2 related sequence 1;
                     antitrypsin; alpha-1 antiproteinase"
                     /codon_start=1
                     /product="serine protease inhibitor A3M isoform 1
                     precursor"
                     /protein_id="NP_033279.2"
                     /db_xref="CCDS:CCDS36538.1"
                     /db_xref="GeneID:20717"
                     /db_xref="MGI:MGI:98378"
                     /translation="MAFIAALGILMAGICPTVLCFSDDTWGIDILLHKNQESGTPDDS
                     LTLASINTDFAFSLYKKMALKNPDKNIVFSPLSISAALALVSLGAKGNTLEEILEGLK
                     FNLTETSEADIHQGFGHLLQRLSQPEDQDQINIGNAMFIEKDLQILAEFHEKTRALYQ
                     TEAFTADFQQPTEATKLINDYVSNQTQGMIKKLISELDDRTLMVLVNYIYFKGKWKIS
                     FDPQDTFESEFYLDEKRSVKVPMMKMKFLTTRHFRDEELSCSVLELKYTGNASALFIL
                     PDQGRMQQVEASLQPETLRKWWKSLKTRKIGELYLPKFSISTDYNLKDILPELGIKEI
                     FSKQADLSGITGTKDLSVSQVVHKAVLDVAETGTEAAAATGFIFGFRSRRLQTMTVQF
                     NRPFLMVISHTGVQTTLFMAKVTNPK"
     exon            709..982
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1133
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1660
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1627..1632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /note="hexamer: AATACA"
     polyA_site      1660
                     /gene="Serpina3m"
                     /gene_synonym="3e46; MMCM7; MMSPi2.4; Spi-2l; Spi-2rs1;
                     spi2; Spi2-rs1; Spi2.4"
                     /note="major polyA site"
ORIGIN      
        1 atgccaggca agccaacaac cctgaacatc aggagtcagc tatcacagag gctctgcaga
       61 ctgcacagaa gatggccttc attgcagctc tagggatctt aatggctggg atctgtccta
      121 ctgtcctctg cttctcagat gacacatggg gaatagatat tctattacat aaaaaccaag
      181 aaagtgggac accagatgac agtctcacat tggcgtccat caacactgac tttgccttca
      241 gcctgtacaa gaagatggct ttgaagaatc cagataaaaa tattgtcttc tccccactta
      301 gcatctcagc tgccttggcc ctcgtgtccc tgggagcaaa gggcaacacc ctggaagaga
      361 ttttagaagg tctcaagttc aatctcacag agacctctga agcagacatc caccagggct
      421 ttgggcacct cctacagagg ctcagccagc cagaagacca ggatcagata aacataggca
      481 atgccatgtt tattgaaaag gacctgcaga tcctggcaga attccatgag aagacaaggg
      541 ctctgtacca gactgaggcc ttcacagcag acttccagca gcctactgag gccacaaagc
      601 tcatcaatga ctatgtgagc aatcagaccc aggggatgat caagaaactg atctcagaac
      661 tggatgatag gacattgatg gtgctggtga attacatcta ctttaaaggc aaatggaaga
      721 tatcctttga cccccaggac acatttgagt ctgagttcta cttggatgag aagagatctg
      781 tgaaggttcc catgatgaaa atgaagtttc tgaccacacg ccacttccgt gatgaggagc
      841 tgtcctgctc tgtgttggag ctgaagtaca caggaaatgc cagcgccctg ttcatcctcc
      901 ctgaccaggg caggatgcag caggtggaag ccagcttaca accagagacc ctgaggaaat
      961 ggtggaaatc tttgaaaacc aggaaaatag gtgagctcta cctgcccaag ttctccatct
     1021 ctactgacta caacctgaag gacatccttc cagagctggg cattaaagaa atcttctcca
     1081 aacaagctga cctatctggg atcacaggaa ccaaggacct gagtgtgtct caggtggtcc
     1141 acaaggctgt gctggacgtg gctgagacag gcacagaagc agctgctgcc acagggttca
     1201 tttttggctt tcgttctaga agattacaaa ctatgactgt gcagttcaac aggccattcc
     1261 tgatggtcat ctctcacaca ggtgttcaga ctaccctctt tatggccaaa gtcactaacc
     1321 ccaagtgaac ctaaagctcc ccaagttctg aatattctgc ccaggaccct aggatggagt
     1381 ccgcatgtgg atctctgttt acatcctggc aatcatgctt tgattggctg atgcaaagat
     1441 ggcttagaca gtgacattga ctatctctgt ggctcccaca tgctcaggag cctttggact
     1501 gtcagtgtca ggcgctcagg cctcttggga gcatctgtgc atgtttctga acctggagtc
     1561 tgtctttatt cctcttccct ggtgactcct ttttatatat ctgtatccca aaactaggca
     1621 ttggcaaata cacagagttc caatacaggc tctgagcaca
//
