LOCUS       NM_001353612            1920 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus serine (or cysteine) peptidase inhibitor, clade A,
            member 3G (Serpina3g), transcript variant 2, mRNA.
ACCESSION   NM_001353612
VERSION     NM_001353612.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Nobs,E., Laschanzky,K., Munke,K., Movert,E., Valfridsson,C. and
            Carlsson,F.
  TITLE     Cytosolic serpins act in a cytoprotective feedback loop that limits
            ESX-1-dependent death of Mycobacterium marinum-infected macrophages
  JOURNAL   mBio 15 (9), e0038424 (2024)
   PUBMED   39087767
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Shamji,M.H., Temblay,J.N., Cheng,W., Byrne,S.M., Macfarlane,E.,
            Switzer,A.R., Francisco,N.D.C., Olexandra,F., Jacubczik,F.,
            Durham,S.R. and Ashton-Rickardt,P.G.
  TITLE     Antiapoptotic serine protease inhibitors contribute to survival of
            allergenic TH2 cells
  JOURNAL   J Allergy Clin Immunol 142 (2), 569-581 (2018)
   PUBMED   29106998
  REMARK    GeneRIF: this study shows that Spi2a contributes to survival of
            allergenic TH2 cells
REFERENCE   3  (bases 1 to 1920)
  AUTHORS   Salazar-Olivo,L.A., Mejia-Elizondo,R., Alonso-Castro,A.J.,
            Ponce-Noyola,P., Maldonado-Lagunas,V., Melendez-Zajgla,J. and
            Saavedra-Alanis,V.M.
  TITLE     SerpinA3g participates in the antiadipogenesis and
            insulin-resistance induced by tumor necrosis factor-alpha in
            3T3-F442A cells
  JOURNAL   Cytokine 69 (2), 180-188 (2014)
   PUBMED   24973688
  REMARK    GeneRIF: SerpinA3g plays a role in the TNF-alpha modulation of
            adipose tissue development and metabolism.
REFERENCE   4  (bases 1 to 1920)
  AUTHORS   Li,L., Byrne,S.M., Rainville,N., Su,S., Jachimowicz,E., Aucher,A.,
            Davis,D.M., Ashton-Rickardt,P.G. and Wojchowski,D.M.
  TITLE     Brief report: serpin Spi2A as a novel modulator of hematopoietic
            progenitor cell formation
  JOURNAL   Stem Cells 32 (9), 2550-2556 (2014)
   PUBMED   24964278
  REMARK    GeneRIF: Authors define roles for an HP-regulated Serpina3g/Spi2A
            intracellular serpin during granulomyelocytic, B-cell, and
            hematopoietic stem cell (HSC) formation.
REFERENCE   5  (bases 1 to 1920)
  AUTHORS   Heit,C., Jackson,B.C., McAndrews,M., Wright,M.W., Thompson,D.C.,
            Silverman,G.A., Nebert,D.W. and Vasiliou,V.
  TITLE     Update of the human and mouse SERPIN gene superfamily
  JOURNAL   Hum Genomics 7 (1), 22 (2013)
   PUBMED   24172014
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1920)
  AUTHORS   Tanaka,K., Tamura,H., Tanaka,H., Katoh,M., Futamata,Y., Seki,N.,
            Nishimune,Y. and Hara,T.
  TITLE     Spermatogonia-dependent expression of testicular genes in mice
  JOURNAL   Dev Biol 246 (2), 466-479 (2002)
   PUBMED   12051830
REFERENCE   7  (bases 1 to 1920)
  AUTHORS   Terskikh,A.V., Easterday,M.C., Li,L., Hood,L., Kornblum,H.I.,
            Geschwind,D.H. and Weissman,I.L.
  TITLE     From hematopoiesis to neuropoiesis: evidence of overlapping genetic
            programs
  JOURNAL   Proc Natl Acad Sci U S A 98 (14), 7934-7939 (2001)
   PUBMED   11438738
REFERENCE   8  (bases 1 to 1920)
  AUTHORS   Hampson,I.N., Hampson,L., Pinkoski,M., Cross,M., Heyworth,C.M.,
            Bleackley,R.C., Atkinson,E. and Dexter,T.M.
  TITLE     Identification of a serpin specifically expressed in multipotent
            and bipotent hematopoietic progenitor cells and in activated T
            cells
  JOURNAL   Blood 89 (1), 108-118 (1997)
   PUBMED   8978283
REFERENCE   9  (bases 1 to 1920)
  AUTHORS   Inglis,J.D., Lee,M., Davidson,D.R. and Hill,R.E.
  TITLE     Isolation of two cDNAs encoding novel alpha 1-antichymotrypsin-like
            proteins in a murine chondrocytic cell line
  JOURNAL   Gene 106 (2), 213-220 (1991)
   PUBMED   1718822
REFERENCE   10 (bases 1 to 1920)
  AUTHORS   Inglis,J.D. and Hill,R.E.
  TITLE     The murine Spi-2 proteinase inhibitor locus: a multigene family
            with a hypervariable reactive site domain
  JOURNAL   EMBO J 10 (2), 255-261 (1991)
   PUBMED   1991447
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AA190062.1, AA727578.1, AC142112.3, AA000746.1, AI153233.1,
            BE851987.1 and AI322743.1.
            
            Transcript Variant: This variant (2) represents an allele found in
            the reference strain. This variant (2) differs in the 5' UTR, lacks
            part of the 5' coding region, and uses a downstream translation
            start site compared to variant 1. The encoded isoform (2) has a
            shorter N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002065.1, SRR13861889.102750.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-142               AA190062.1         1-142
            143-354             AA727578.1         141-352
            355-453             AC142112.3         59247-59345         c
            454-868             AA000746.1         1-415
            869-1130            AI153233.1         204-465
            1131-1484           AC142112.3         55950-56303         c
            1485-1903           BE851987.1         11-429              c
            1904-1920           AI322743.1         343-359
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 53.71 cM"
     gene            1..1920
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /note="serine (or cysteine) peptidase inhibitor, clade A,
                     member 3G"
                     /db_xref="GeneID:20715"
                     /db_xref="MGI:MGI:105046"
     exon            1..172
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /inference="alignment:Splign:2.1.0"
     exon            173..579
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             549..1295
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine (or cysteine) proteinase inhibitor, clade A, member
                     3G; serine protease inhibitor A3G; serpin 2A; serpin A3G;
                     serine protease inhibitor 2A; serine protease inhibitor
                     2-1; alpha-1 antiproteinase"
                     /codon_start=1
                     /product="serine protease inhibitor A3G isoform 2"
                     /protein_id="NP_001340541.1"
                     /db_xref="CCDS:CCDS88397.1"
                     /db_xref="GeneID:20715"
                     /db_xref="MGI:MGI:105046"
                     /translation="MVLVNYIYFKGKWKNPFDPNDTFKSEFYLDEKRSVIVSMMKTGY
                     LTTPYFRDEELSCTVVELKYTGNASAMFILPDQGRMQQVEASLQPETLRKWKNSLKPR
                     MIHELRLPKFSISTDYSLEHILPELGIREVFSTQADLSAITGTKDLRVSQVVHKAVLD
                     VAETGTEAAAATGMAGVGCCAVFDFLEIFFNRPFLMIISDTKAHIALFMAKVTNPERS
                     MNFPNGEGASSQRLESKRLCFGDPLCLIGQ"
     exon            580..853
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1004
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1908
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1886..1891
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /note="hexamer: AATAAA"
     polyA_site      1908
                     /gene="Serpina3g"
                     /gene_synonym="2A2; spi2; Spi2-1; Spi2/eb.1; Spi2A"
                     /note="major polyA site"
ORIGIN      
        1 ttagagtcca gagcacagct tatctgcatc aacatttcct aagatgtggg aggagtttgg
       61 ttcggataaa taattcagca tgcagtggag agaacagcag accaggaagg cagcagccct
      121 gaccaccagg agccagatat cacagacact ggcagctggc tcagctctgc agcatctgca
      181 ctgccttggc cctcctgtcc ctgggagcaa agagcaacac cctgaaggaa atcctagaag
      241 gtctcaagtt caacctcaca gagacccctg aaccagacat ccaccagggc tttaggtact
      301 tgctagacct tctaagtcag ccagggaacc aggtacagat cagcacaggc agtgccctgt
      361 ttattgaaaa gcacctacag atcctggcag agttcaagga gaaggcaagg gctctgtacc
      421 aggctgaggc cttcacagca gatttccagc aacctctcaa ggccacaaag ctcatcaatg
      481 actatgtgag caatcacacc caggggaaga tcaaggaact catctcaggc ctgaaagaga
      541 gcacattgat ggtgctggtg aactacatct actttaaagg caaatggaag aacccctttg
      601 acccgaatga tacatttaag tccgagttct acttggatga gaagaggtct gtgattgtgt
      661 ccatgatgaa aactggttac ctgacaactc cctacttccg ggatgaggag ctgtcctgca
      721 ctgtggtgga gctgaaatac acaggaaatg ccagtgccat gttcatcctc cctgaccagg
      781 gcaggatgca gcaggtggaa gcaagcttac aaccagagac cctgaggaag tggaagaact
      841 ctctgaagcc caggatgata catgagctcc gcctgcccaa gttctccatc tccaccgact
      901 acagcctgga gcacatcctt cctgagttgg gcatcaggga agtcttctcc acacaggctg
      961 acctgtctgc aatcacagga accaaggatc tgagagtctc tcaggtggtc cacaaggctg
     1021 tgctggatgt ggctgagaca ggcacagagg ctgctgctgc cacaggaatg gcaggtgtcg
     1081 gatgttgtgc agtttttgac tttctggaaa tatttttcaa caggccattc ctgatgatta
     1141 tctctgacac aaaagctcac attgccctct ttatggcaaa agttacaaat ccagagagat
     1201 ctatgaactt cccaaatggt gagggtgctt cttcccaacg gctggaatct aagcgtttat
     1261 gttttggtga tccgctatgc ctgattggac agtgacactt accaactctg tcacgtcctc
     1321 atgcagagaa agaagcctat ggctggttat tgtcagactc tctgtcatga tggtatcacg
     1381 aaatcaggtt cctgacctga aatttgtgtt attccctttc cctgctgtct ccattgtatc
     1441 tgcctcaacc caaaagcctg ggcccagcag tgaggctctc tcctaactta ggctctgctt
     1501 atgtctgcct tcatcctttc agtcatgatg ggactgtgca agtttacagg ccaacccata
     1561 aggactaaga aaggaacctg gtgccctggg tctatctgca gcatctgaaa tgcttggtgc
     1621 ccagatctgc cttatgcttg ccttcctctg ggcagaatta tcctcagccc ctgcatagtc
     1681 tcctggcccc acccagatct ggcacaggtg gagatctcag ccctgcagct gcatggggcc
     1741 tgtgggtcag agcagctcct ccccctgtag cactcctact cagaacagag tagtctttct
     1801 caatgtctct tgttgaccaa ctacgtagga ctggtggaca cctctgcatt catcacacaa
     1861 attgaaaggg caggagctaa ggatcaataa acatgtaact gcattgaaaa aaaaaaaaaa
//
