LOCUS       NM_011354               3072 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus small EDRK-rich factor 2 (Serf2), transcript variant
            2, mRNA.
ACCESSION   NM_011354
VERSION     NM_011354.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Stroo,E., Janssen,L., Sin,O., Hogewerf,W., Koster,M., Harkema,L.,
            Youssef,S.A., Beschorner,N., Wolters,A.H., Bakker,B., Becker,L.,
            Garrett,L., Marschall,S., Hoelter,S.M., Wurst,W., Fuchs,H.,
            Gailus-Durner,V., Hrabe de Angelis,M., Thathiah,A., Foijer,F., van
            de Sluis,B., van Deursen,J., Jucker,M., de Bruin,A. and Nollen,E.A.
  TITLE     Deletion of SERF2 in mice delays embryonic development and alters
            amyloid deposit structure in the brain
  JOURNAL   Life Sci Alliance 6 (7), e202201730 (2023)
   PUBMED   37130781
  REMARK    GeneRIF: Deletion of SERF2 in mice delays embryonic development and
            alters amyloid deposit structure in the brain.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Cleverley,K., Lee,W.C., Mumford,P., Collins,T., Rickman,M.,
            Cunningham,T.J., Cleak,J., Mianne,J., Szoke-Kovacs,Z., Stewart,M.,
            Teboul,L., Maduro,C., Wells,S., Wiseman,F.K. and Fisher,E.M.C.
  TITLE     A novel knockout mouse for the small EDRK-rich factor 2 (Serf2)
            showing developmental and other deficits
  JOURNAL   Mamm Genome 32 (2), 94-103 (2021)
   PUBMED   33713180
  REMARK    GeneRIF: A novel knockout mouse for the small EDRK-rich factor 2
            (Serf2) showing developmental and other deficits.
REFERENCE   3  (bases 1 to 3072)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 3072)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   5  (bases 1 to 3072)
  AUTHORS   Scharf,J.M., Endrizzi,M.G., Wetter,A., Huang,S., Thompson,T.G.,
            Zerres,K., Dietrich,W.F., Wirth,B. and Kunkel,L.M.
  TITLE     Identification of a candidate modifying gene for spinal muscular
            atrophy by comparative genomics
  JOURNAL   Nat Genet 20 (1), 83-86 (1998)
   PUBMED   9731538
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ073462.1, AK132746.1 and BP768998.1.
            
            On Mar 19, 2014 this sequence version replaced NM_011354.2.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' terminal exon, which results in a frameshift in the 3'
            coding region, compared to variant 1. The encoded isoform (b) has a
            distinct C-terminus and is shorter than isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.20720.1,
                                           SRR1660819.189527.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-410               CJ073462.1         7-416
            411-2638            AK132746.1         841-3068
            2639-3072           BP768998.1         30-463              c
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.39 cM"
     gene            1..3072
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /note="small EDRK-rich factor 2"
                     /db_xref="GeneID:378702"
                     /db_xref="MGI:MGI:1337041"
     exon            1..244
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..417
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /note="isoform b is encoded by transcript variant 2;
                     protein 4F5-related; 4F5rel (4F5 related)"
                     /codon_start=1
                     /product="small EDRK-rich factor 2 isoform b"
                     /protein_id="NP_035484.1"
                     /db_xref="CCDS:CCDS16645.1"
                     /db_xref="GeneID:378702"
                     /db_xref="MGI:MGI:1337041"
                     /translation="MTRGNQRELARQKNMKKQSDSVKGKRRDDGLSAAARKQRDSEIM
                     QQKQKKANEKKEEPK"
     exon            245..353
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /inference="alignment:Splign:2.1.0"
     exon            354..3053
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      669..674
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /note="hexamer: ATTAAA"
     polyA_site      695
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /note="major polyA site"
     regulatory      2619..2624
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
                     /note="hexamer: AATAAA"
     polyA_site      2659
                     /gene="Serf2"
                     /gene_synonym="m4F5rel; Msmac1l"
ORIGIN      
        1 gaagccccgc cccgtgccag cgctccgccc gccgagctcc gctcttcgcc ctccgggact
       61 ggcgcgtggc accgcactcc acgtgtttcc ccctgcccaa tgaaagcggg cgcccgggcg
      121 tgaaggggcg ggaggaagaa gacgacttgg tggcgtcgcg cgaagggggc ggggcctgca
      181 acgtcggccc gaacgagggg gacgcaacgg aggcaggttg gagccgctgc cgtcgccatg
      241 acccgcggta accagcgaga gctcgcccgc cagaagaaca tgaagaagca gagcgactcg
      301 gttaagggaa agcgccgaga tgatgggctt tctgctgccg cccgcaagca gagggactcg
      361 gagatcatgc agcagaagca gaaaaaggca aacgagaaga aggaggaacc caagtagcct
      421 tgtggcttcg tgtccaaccc tcttgccctc cgcctgtgtg cctggagcca gtcccaccat
      481 gctcgagttt cttcctgtag tgctcacagg tcccagcacc gatggcattc cctttgccct
      541 gagtctgcag cgggttcctt ttgtgcttcc ttcccctcag gtagcctctc tccctctggg
      601 ccactcctgg gggtgagggg gttacccctt cccagtgttt tttattcctg tggggcacac
      661 cccaaagtat taaaagtagc tttgtaattc cttgagcgcc tggtttgact ggggattggg
      721 ggcatgggga tggaggagtg actgcccttc cccactaaaa ggggggagtt ttgttaggca
      781 cctaagagat tatggatttg tagacttcat aaggaaaaca tcacagaaga agcctttatt
      841 ttaactattg caaccggaaa agcactccag ttatatggca gaggagtcct ctaagaacta
      901 tagagacttc ttttctgtga tttttatccc ccacaccgac cttggacacc atccccagga
      961 tggtattgag ggtcataaga gggaaggatc taagacccaa atttatgtct cttcattctt
     1021 tgtttgcttg aaagttggcc aaaaaaaaaa atcctgctgc tcaccaactt ctggtggtca
     1081 gccactctgg atgctcacat tctcttctgt tctgtcaatc ctcatggaat gaaggtggtt
     1141 tttgtcttcc atttcctttg acctcaagac caggattaaa acctatggta gcctctgctc
     1201 ccttcttccc ctgccctggt ttgttctgtg gtctgggctt cttccaccac tgcttagggc
     1261 tgagctactc atttcctttt gattgctaag agcagaccca aatcttcaat ctctgtaggt
     1321 ctttccctga ccttatctcc agccctggaa ccagaaaggg tcttgtgcca cactgctagg
     1381 atcaggtaag gctcctgggt ttacttcctg ggagttagac tcttgagctt taatgaacta
     1441 gtgagtttgt ctgcttcttc aaatttagat acttactgag cctatgcacc gataactgct
     1501 catttgtcct tgaggtattg agttcagctg ggtgttcctg gagctaagat taagagcata
     1561 atctaaaggg tctgtctccc agtttgttac tacagatcaa gagccagttt ttatgtggca
     1621 gtactgatgc catggcagtg ttgcctgaat atactagggt ggtggtgatt ctgagtgggg
     1681 agccagcagt ggtgccagga gcagccccag atagaggagg acacaggccc agcatgaggc
     1741 aactttaacc cagaaggtag cccagctacc cttagtgaag gtcttctcca gttctgcttc
     1801 ctcatagctg tgaaaccaaa ggctgattag agatgatgca gggccttagc ttttgaacct
     1861 ccctacttcc tcataaccag ggcagatcct tgtgtgtctc tcccaagaac ttttggaatg
     1921 gccttaaagt gtttccttaa aattgctaga ttttcaggag agtatcttat gtctgctttc
     1981 ttcttacctg aaccagttgg taagggtaac catgacatag agtgaagcaa ggaagaaggc
     2041 gaagtggaag ccagagtagc tgtaggaaag atgctggctc tgcacctgag cagctggagg
     2101 ggtctcttgg tcagctgacc tggccctgct cctttgccct agagagagaa gtgccgatgg
     2161 ggagcaatgt tgctgacact cccttccccc aactattgtg aaggctcttg agggctctgg
     2221 cccttcacag ggttcagctt ggctagattg cgcatgtgac tcaccatcct ctggctccac
     2281 tgtttggggg cagcagaaac agactgaggg cttctgtaaa acaaggcaac acgttttgaa
     2341 atggctttaa gacagtgtga gggtgctaat ttagaaaatc tcccctttga aaagattgac
     2401 ctggtgtaag aagtggaggc acacctgggt tcaaattctt gctccagcat ataactggct
     2461 atgcaaactt tggtgagatg tttaatcttt tgtgcctcaa ttcctccatt tgtgaaatgg
     2521 agaaaatacc tacctcacag ggttgttgtg aggatgaaat gaaatgagac tatgtatgta
     2581 aagtatctag cacagtgcct agcacattgt gggtactcaa taaaaggaaa cagcagctag
     2641 aatctgagca ttctgggtag aagttggtag gatgtgtgtc tctgtgtttg ggggagggtt
     2701 gttggggttt tgagctcttg atgctcacct ggaactcata cttgtaaacc ttgataatcc
     2761 acaagggtcc aaataactcg gccaggtagg aagcctcgtt gctgtgaaag aaagaggttt
     2821 ttaaaccttg ggccaaatgt gaaagattgg tagcatttac tgtcaacatc cttctggagg
     2881 gtattgtgca ctcagaggcc cctaaagtct gtgcatttta aacattcttt cctgcccccg
     2941 tcatctcatg cagatcatct gacaaagtgc tattcttaga aattgtccct gttcgtgggg
     3001 agcttttctg gttctagact tgctctacag tttaaataaa cctcagcact ggaaaaaaaa
     3061 aaaaaaaaaa aa
//
