LOCUS       NM_172490               5243 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA
            synthase (Sepsecs), transcript variant 1, mRNA.
ACCESSION   NM_172490
VERSION     NM_172490.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Fradejas-Villar,N., Zhao,W., Reuter,U., Doengi,M., Ingold,I.,
            Bohleber,S., Conrad,M. and Schweizer,U.
  TITLE     Missense mutation in selenocysteine synthase causes
            cardio-respiratory failure and perinatal death in mice which can be
            compensated by selenium-independent GPX4
  JOURNAL   Redox Biol 48, 102188 (2021)
   PUBMED   34794077
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 5243)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5243)
  AUTHORS   Mix,H., Weiler-Normann,C., Thimme,R., Ahlenstiel,G., Shin,E.C.,
            Herkel,J., David,C.S., Lohse,A.W. and Rehermann,B.
  TITLE     Identification of CD4 T-cell epitopes in soluble liver
            antigen/liver pancreas autoantigen in autoimmune hepatitis
  JOURNAL   Gastroenterology 135 (6), 2107-2118 (2008)
   PUBMED   18773898
REFERENCE   5  (bases 1 to 5243)
  AUTHORS   Ganichkin,O.M., Xu,X.M., Carlson,B.A., Mix,H., Hatfield,D.L.,
            Gladyshev,V.N. and Wahl,M.C.
  TITLE     Structure and catalytic mechanism of eukaryotic selenocysteine
            synthase
  JOURNAL   J Biol Chem 283 (9), 5849-5865 (2008)
   PUBMED   18093968
REFERENCE   6  (bases 1 to 5243)
  AUTHORS   Xu,X.M., Carlson,B.A., Mix,H., Zhang,Y., Saira,K., Glass,R.S.,
            Berry,M.J., Gladyshev,V.N. and Hatfield,D.L.
  TITLE     Biosynthesis of selenocysteine on its tRNA in eukaryotes
  JOURNAL   PLoS Biol 5 (1), e4 (2007)
   PUBMED   17194211
REFERENCE   7  (bases 1 to 5243)
  AUTHORS   Small-Howard,A., Morozova,N., Stoytcheva,Z., Forry,E.P.,
            Mansell,J.B., Harney,J.W., Carlson,B.A., Xu,X.M., Hatfield,D.L. and
            Berry,M.J.
  TITLE     Supramolecular complexes mediate selenocysteine incorporation in
            vivo
  JOURNAL   Mol Cell Biol 26 (6), 2337-2346 (2006)
   PUBMED   16508009
REFERENCE   8  (bases 1 to 5243)
  AUTHORS   Xu,X.M., Mix,H., Carlson,B.A., Grabowski,P.J., Gladyshev,V.N.,
            Berry,M.J. and Hatfield,D.L.
  TITLE     Evidence for direct roles of two additional factors, SECp43 and
            soluble liver antigen, in the selenoprotein synthesis machinery
  JOURNAL   J Biol Chem 280 (50), 41568-41575 (2005)
   PUBMED   16230358
  REMARK    GeneRIF: SECp43 and SLA have roles in selenoprotein synthesis
            [soluble liver antigen]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC120128.17.
            
            On May 19, 2018 this sequence version replaced NM_172490.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049338.1, SRR10223970.7447.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC120128.17        23133-23259
            128-282             AC120128.17        23781-23935
            283-401             AC120128.17        26728-26846
            402-560             AC120128.17        27440-27598
            561-714             AC120128.17        28513-28666
            715-817             AC120128.17        32102-32204
            818-947             AC120128.17        36479-36608
            948-1039            AC120128.17        36729-36820
            1040-1133           AC120128.17        45143-45236
            1134-1224           AC120128.17        45628-45718
            1225-5243           AC120128.17        48727-52745
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 28.44 cM"
     gene            1..5243
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /note="Sep (O-phosphoserine) tRNA:Sec (selenocysteine)
                     tRNA synthase"
                     /db_xref="GeneID:211006"
                     /db_xref="MGI:MGI:1098791"
     exon            1..127
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..1528
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /EC_number="2.9.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-phosphoseryl-tRNA(Sec) selenium transferase;
                     selenocysteinyl-tRNA(Sec) synthase; UGA suppressor
                     tRNA-associated protein"
                     /codon_start=1
                     /product="O-phosphoseryl-tRNA(Sec) selenium transferase
                     isoform 1"
                     /protein_id="NP_766078.1"
                     /db_xref="CCDS:CCDS19286.1"
                     /db_xref="GeneID:211006"
                     /db_xref="MGI:MGI:1098791"
                     /translation="MNPESFAAGERRVSPAYVRQGCEARRAHEHLIRLLLEQGKCPED
                     GWDESTLELFLHELAVMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDIS
                     AVQPKAAGSSLLNKITNSLVLNVIKLAGVHSVASCFVVPMATGMSLTLCFLTLRHKRP
                     KAKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLEGDELRTDLKAVEAKIQELGPEHIL
                     CLHSTTACFAPRVPDRLEELAVICANYDIPHVVNNAYGLQSSKCMHLIQQGARVGRID
                     AFVQSLDKNFMVPVGGAIIAGFNEPFIQDISKMYPGRASASPSLDVLITLLSLGCSGY
                     RKLLKERKEMFVYLSTQLKKLAEAHNERLLQTPHNPISLAMTLKTIDGHHDKAVTQLG
                     SMLFTRQVSGARAVPLGNVQTVSGHTFRGFMSHADNYPCAYLNAAAAIGMKMQDVDLF
                     IKRLDKCLNIVRKEQTRASVVSGADRNKAEDADIEEMALKLDDVLGDVGQGPAL"
     exon            128..282
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            283..401
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            402..560
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            561..714
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            715..817
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            818..947
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1039
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1133
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1224
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..5243
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4137..4142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /note="hexamer: AATAAA"
     polyA_site      4156
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /note="major polyA site"
     regulatory      5189..5194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
                     /note="hexamer: AATAAA"
     polyA_site      5217
                     /gene="Sepsecs"
                     /gene_synonym="D5Ertd135e; SecS; SLA"
ORIGIN      
        1 agtgggcgcc gtcatgaacc cggagagctt cgcggcgggc gagcggcggg tgtctccggc
       61 ttacgtgagg cagggctgcg aggctcggcg cgcccacgag cacctcatcc ggctgcttct
      121 agagcagggc aagtgtccag aggatggctg ggatgaaagt accctcgaac tctttctgca
      181 tgagcttgca gtcatggata gcaacaattt cttgggtaat tgtggtgtgg gagaaaggga
      241 agggagagtg gcctctgctt tggttgctcg acgacattac aggttcatcc acggaattgg
      301 acgatccggt gatatttctg ctgtgcaacc aaaagctgca ggctctagcc tcttgaacaa
      361 aattactaat tctttggtgc ttaatgtcat aaagcttgct ggtgttcatt cagtggccag
      421 ttgctttgta gttcctatgg caactgggat gagtctaacc ttgtgtttcc tgacactacg
      481 acacaaaaga ccaaaggcca agtatatcat atggccacgg atagaccaga agtcctgctt
      541 taagtccatg gtcactgcag gttttgaacc cgtggtaata gaaaacgtat tggaaggtga
      601 tgagctgcgc acagacctga aagccgtgga ggctaaaatc caggagctcg ggccggagca
      661 catcctgtgc cttcattcta ccacagcctg ctttgctcca agggtgcccg atagattaga
      721 agaactggct gtgatttgtg ctaattatga cattccacat gtagtcaaca acgcttatgg
      781 attacagtct tcaaagtgca tgcatctcat tcagcagggg gctcgggttg gtagaattga
      841 tgctttcgtt cagagcttgg acaaaaattt tatggttcca gtaggtggtg ctataattgc
      901 tggctttaat gagcccttca ttcaggacat cagcaagatg tatccaggaa gagcctcagc
      961 ctcgccgtcc ttagacgtcc tcatcacctt gctgtcactg ggctgcagtg gctacaggaa
     1021 gctgttgaag gagagaaagg aaatgtttgt ctatttatcc acccaactaa agaagttggc
     1081 agaggcccac aatgaaagac tactacagac acctcacaac cctatatcct tagctatgac
     1141 acttaagacg atagatggac accatgacaa agctgtcact cagcttggct caatgctttt
     1201 taccagacaa gtgtctggag ccagggctgt gcctcttggg aacgtgcaaa ctgtgagtgg
     1261 ccatactttt cgaggcttca tgtcccatgc agataattac ccctgtgctt acctcaatgc
     1321 ggcagcagcc atcgggatga agatgcagga tgtggacctg ttcataaaga gacttgacaa
     1381 gtgcttaaac atagtaagga aagaacagac tagagcaagt gttgtgtctg gagctgaccg
     1441 caacaaagct gaagatgcgg acattgagga aatggctttg aaactggatg acgtgcttgg
     1501 ggatgtgggc cagggccctg ctctatgaca cagggctctt tttgacagtt catacaatgt
     1561 atgtaaagtt tttgaatcaa gaattttata gagagtattc tgccaaggaa gagactggtc
     1621 tgagggtgat tgctttttat ccttagattg cctcagatga tgcttactaa tagaaaatgt
     1681 aaagctgcag gtgattctca ctcagataca gagagcttct gagccattcg tcagcattca
     1741 gaggttgttt gttggtttgt ttccaatcac acagaattga gaaagatctg tttctaatgc
     1801 tgcttaacac cagatgttta tgtagcagtc tctgtgccta ttgaaaggat gtttcccaag
     1861 gaaacagggc catgtcatag gcttaagagc tggcttcgtt atatataaac agcatggaaa
     1921 gactcacagt gggtgctagt ggcgttacct ggtagaaccc agcagttctg ctcagcacct
     1981 cacctactgc ccaacacata gtcctcagaa gaattctcca gtccaggaca tcagtagtga
     2041 tgtgttttag ggaagcgagc tccccagtga aagaggagtt accacatgtg ctgcctgatg
     2101 gatgacactg ctgtttgtgg tgggctgcat ggaaatcagg ggtcccatga gagcagtgcc
     2161 tatcgatgtg gtggatgtca cagtttgttc aggtgtgtgc catgctgttc atatacctat
     2221 aaaatcagct aattacatgt tttagaccat atctcagtga tatgtgcctg caaaaatttt
     2281 aaaaagttag gtctgtgaat gttactaggg ctacttggtc gagactgtta gtgacaataa
     2341 gagtcactag ttgcaaacaa gtttctacaa gttgttttct ataatacctg ctcatttttc
     2401 catttgggca actgcaaact ttattccaca tactgtctgg gctttaagca gcctttgtga
     2461 ctacatatat atgaatgact gaaattctag gccccatacc tctgtgtttt ttgttagtta
     2521 tacacaacaa aacaaacaaa aaaacaaaca aacagaagaa caaaacaaaa aaacaactag
     2581 actgggggct tagtgaagcc tcttgggaac agccatgcca aagctatgtt tcttaggcct
     2641 gctctgtgga gcgtcactct acccaaaaca tagccatggg gaaagggccc atggaggaat
     2701 cattcgggga aatgcctttg taaatttgct catggtgtat taaatgctac aagaggattt
     2761 ctttagtaaa attggcctgc ttttccaaag aggagccggt tctctgaaat gaaacactct
     2821 ttgtacatac tctcccaaca caatgggtca aatgtgttaa cttctggatt ctactcttgt
     2881 attggtatga cactgagctg tatatgaaaa gggagtcttc cttgcctacg atcttagaag
     2941 gtttgtacac atgagcttag ccagaagctg ttttgagtgc gtaagttggg ccatgtgagc
     3001 cactgatgca cctgcaagtc gaggctggat aaagaactca ctggtaaatg tattctacct
     3061 ttggggacca gacactaaaa tggctcttag gtagctgtag taactcgtta catgggagta
     3121 aacctggatt gaaaggctct gtgctgtttt ctgtgtggat tggggactgc accataaatg
     3181 agatgtagcc acaaaggccc cgtctctgtg gggcctgtga gggtcagatg ctgctgccga
     3241 tggaattgct ggcagtgcag cttcctacgc atcaggccaa aatgattttc tagaacaagt
     3301 gatctatatt gtgtgatcat cattttgctt aagaaaggcc agagtccaga gactggagat
     3361 gcttcctcat ctgccgagta tcagcagttc ttggcttcag ctgctgaata gataataatt
     3421 ctaattgaag ataaattaca tgaacccaca tgtgaatgat agatatggta caacatttta
     3481 ttgctggtgc ataaaatccg ctgcagtttt ggttattaac cctttccttt ttaagcactt
     3541 ttaaattcca gctctgttct tcctagctcc ggcttgagcc taaggtcagg tttttctccc
     3601 ccaaccctgt atgacttacc taagtctctc tcctgttcac atctgcatgt tagaaggact
     3661 gttcctatct ctgcgggaag cacggacagt ccttgggatg cttcctgatg ttcccgtgtt
     3721 gccctcaggc tgtggcagtg gagggtaccg ggcaggcatt gcctaaggcc ctactactgt
     3781 ctctgtggcc caactcctct tacagtaggg ccatgaagta gactgttgga gattggcttt
     3841 taaagtcact gggctggagt agtctgccta gagaagaggt ttggtcacta acacatctta
     3901 ctcacattgc agggagaggt gttttcagtt gagtcttagt ttgccctagg tccctgccga
     3961 tgtgttctca ctgtcaggga aagatatgac agggccttgt aagccagtgc taactcggaa
     4021 tcttcctact ggctcctggt aatagttggt ctcagttata caagatgagt tatgtaatac
     4081 agttctcttc caccaatagg ctttgatgtg caattggtaa atttaaatat catttaaata
     4141 aatatttttc taataacttg gcccagcact taattattca ctgattctat ttctttaccc
     4201 acaagacaag aagaactgtc actgaggccg tagagacaga caatgtctat gctctggctt
     4261 ccatagtaaa actttgacaa attctcatgt tttaaacagg tggtgatact gagtaagtgc
     4321 cctcatcctg acaagctcag ctttgagtct tttcactaaa gtaggcaatc aacaatcgaa
     4381 aataacagta atgaaaacca gtgctgagac tcattcatat tgttctcaga gaaaaatttg
     4441 aaatacacac acatttggtg cagataaaaa ttggatttac gttgcctccc aatctagggc
     4501 gcactagttt ttgccaagct ggaaaatggt tttgttttac gttgatgaga tcgactttat
     4561 atattgagtt ggaagaaacc cagttttaca tgcgcagaag ttggttaatt tcagatattt
     4621 ttataatctt tgaataattt ggtaaccaat tggccatgca agtagcagaa gtgcatgtat
     4681 gtatgcatgg gcatgcgtgt ctgcgtcgag gactcgtcgg acaggtgcta ggtgcctcct
     4741 tgtctgtttt tgttggttct gctctttggc caaccttagt ctgcaggtct gatttgatgc
     4801 cttctttatg ccttacattt tacttttatt ttggcttcat ggtctgtgtg acataaaatg
     4861 atgattttac agctaatgaa tattactttg taactaacca gctaatattc tgcagttcaa
     4921 gctaaggcca cagacaaagt cagattctgt ttataccata gttttgtttt gttttgtttt
     4981 aaatcaaata tgccaacagg agcctttggg gaagaggtga aggaaggcta agtctaaccg
     5041 tgtaagtggg tgtgggggtt aagctatgag actgtgatgt ggacatcaat ctcctgacct
     5101 gtatattacc aaagggagaa acaattccag aaatattttt ggtatagact gtttaaaata
     5161 aaaacaaatt gtataattta ttacatgaaa taaattttta ataaatcatt gactacagat
     5221 gaaatctgga agtgcaaata atc
//
